Disposable PD-10 Desalting Columns Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex G-25 Medium. Flexible protocols allow runs by gravity flow or centrifugation. Easy to use and equilibrate; apply sample; elute. Preparation of the samples in the range of 1.0 to 2.5 ml by gravity flow and 1.75 to 2.5 ml with centrifugation. Desalt multiple samples in parallel with a desalting capacity > 90%. Recovery in the range 70 to >95%. Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.

Disposable PD-10 Desalting ColumnsBed Dimensions 14.5 50 mm

Bed Volume 8.3 ml

Sample Volume 2.5 ml

Complete Packsize 30 Columns

Storage Conditions 4 to 30C, 0.15% Kathon CG/ICP Biocide

Guidance Disposable columns for clean-up of proteins/oligosaccharides.

The columns are prepacked with Sephadex G-25 medium and for sample volumes up to 2.5 ml by gravity flow and columns spin flow

1 x Column stand4 x PD-10 spin adaptor1 x Buffer tray30 x Bottom sleeveInstructions are supplied with the columns

The white boxes in the picture are to secure safe transport of the columns

PD- 10 Buffer reservoir has to be ordered separately

For smaller scale please use PD MidiTrap G-25 (1.0 ml), PD MiniTrap G-25 (0.5 ml), PD SpinTrap G-25 (130 l) and PD MultiTrap G-25 (130 l in parallel)

Wetted Part No

Media

BioProcess MediumYes

MatrixCross-linked dextran

Particle Size Wet38 m-235 m

Partical Size Dry>50 m

pH Stability Working Range2-13

pH Stability Cleaning2-13

Pressure/Flow Specification>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

Fractionation [Mr] Globular Proteins1 x 103 5 x 103

Fractionation [Mr] Dextrans1 x 102-1 x 102

Exclusion Limit [Mr] [Globular Proteins]> 5 x 103

Exclusion LimitMr 5000

Chemical StabilityAll commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Column

Column i.d.15 mm

Chemical StabilityStable in all commonly used buffer systems

Material [Column Hardware]Polypropylene (PP)

Material [Bed Support]Polyethylene (PE)