Discover your full protein potential with great offers for your lab

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Dear Researcher,

We are pleased to share with you our annual end-of-year promotions* offering great bundle prices on more than 70 popular Thermo Scientific™ and Invitrogen™ protein biology products.

This is the perfect occasion for you to purchase high-quality products for your protein studies and get the most from your remaining budget.

The Thermo Scientific Protein Biology Team

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Table of contents

Sample preparation

1.1. Protease and phosphatase inhibitor cocktails; liquids and tablets 051.2. Dialysis devices 061.3. Concentration devices 081.4. Desalting devices 10

Protein electrophoresis – protein ladders

2.1 Unstained protein ladders 122.2 Prestained and PageRuler protein ladders 132.3 Multicolour protein ladders 142.4 Western blot chemiluminescent and near infrared ladders 152.5 Mini gel tank for protein electrophoresis 16

Western blotting apparatus and reagents

3.1 Dry transfer apparatus and reagents for western blot 183.2 Semi automated apparatus and reagents for western immunoblotting 193.3 Chemiluminescent reagents 203.4 X-ray films for chemiluminescence 213.5 Western blot stripping buffers 22

0411 Sample preparation

Sample preparation is an essential stage in the analysis process. Depending on the type of sample, there are various ways to prepare it and get it ready for measurement. On the next few pages, you will find some of our top sample preparation products.

1.1 Protease and phosphatase inhibitor cocktails; liquids and tablets

1.2 Dialysis devices1.3 Concentration devices1.4 Desalting devices

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Highlights:

Efficient: broad-spectrum protease and phosphatase inhibitor cocktails

Ready to use: liquid formulation can treat non-standard sample volumes; the refrigerator-stable tablets dissolve quickly when added to buffers and lysis reagents

Compatible: compatible with Thermo Scientific™ Pierce™ Protein Extraction Reagents and most other commercial or homemade cell lysis solutions

1.1 Protease and phosphatase inhibitor cocktails; liquids and tablets

Thermo Scientific™ Inhibitor Cocktails offer all-in-one solutions for both protease and phosphatase inhibitors. For more convenience, you can now choose between liquid and tablet formats.

A complete solution for your protein sample protection

Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

87785X4 15513237 4 x Halt™ Protease Inhibitor Cocktail, EDTA-free (100x), 1 mL 304 228

78437X4 15513227 4 x Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100x), 5 mL 880 660

78441X4 15523227 4 x Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100x), 1 mL 696 522

78445X4 15533227 4 x Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100x), 5 x 1 mL 1712 1284

88666X4 15593247 4 x Pierce™ Protease Inhibitor Mini Tablets, EDTA-free, 30 tablets 268 201

88667X4 15503257 4 x Pierce™ Phosphatase Inhibitor Mini Tablets, 20 tablets 392 294

88669X4 15513257 4 x Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free, 20 tablets 508 381

88266X4 15523237 4 x Pierce™ Protease Inhibitor Tablets, EDTA-free, 20 tablets 736 552

87735X4 15593227 4 x Slide-A-Lyzer™ G2 Dialysis Cassettes 20K MWCO, 3 mL, 10 cassettes 396 297

87736X4 15503237 4 x Slide-A-Lyzer G2 Dialysis Cassettes 20K MWCO, 15 mL, 8 cassettes 496 372

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1.2 Dialysis devices

Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes enable simple and effective dialysis without the hassle of conventional tubing.

Make dialysis even easier and use the Thermo Scientific™ Slide-A-Lyzer™ G2 Dialysis Cassettes with easy pipette loading and self-floating system.

A unique concept for fast and safe dialysis

Highlights:

Reliable: designed to maintain the highest possible sample integrity and protection

Fast and consistent: dialysis with maximum sample recovery

Convenient: Slide-A-Lyzer G2 Dialysis Cassettes are extra convenient to use: self floating chambers for buoyancy and vertical orientation during dialysis; a twist-off cap provides pipette-friendly, easy sample loading and retrieval

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For more information visit thermofisher.com/proteinoffer

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Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

66205X4 15553217 4 x Slide-A-Lyzer Dialysis Cassettes 2K MWCO, 0.2-0.5 mL, 10 cassettes 304 288

66203X4 15543217 4 x Slide-A-Lyzer Dialysis Cassettes 2K MWCO, 0.5-3 mL, 10 cassettes 304 288

66333X4 15573217 4 x Slide-A-Lyzer Dialysis Cassettes 3.5K MWCO, 0.1-0.5 mL ,10 cassettes 412 309

66330X4 15563217 4 x Slide-A-Lyzer Dialysis Cassettes 3.5K MWCO, 0.5-3 mL, 10 cassettes 412 309

66110X4 15533217 4 x Slide-A-Lyzer Dialysis Cassettes 3.5K MWCO, 3-12 mL, 8 cassettes 484 363

66370X4 15583217 4 x Slide-A-Lyzer Dialysis Cassettes 7K MWCO, 0.5-3 mL, 10 cassettes 412 309

66383X4 15503227 4 x Slide-A-Lyzer Dialysis Cassettes 10K MWCO, 0.1-0.5 mL, 10 cassettes 412 309

66380X4 15593217 4 x Slide-A-Lyzer Dialysis Cassettes 10K MWCO, 0.5-3 mL, 10 cassettes 404 303

66003X4 15523217 4 x Slide-A-Lyzer Dialysis Cassettes 20K MWCO, 3 mL, 10 cassettes 304 288

87729X4 15553227 4 x Slide-A-Lyzer G2 Dialysis Cassettes 10K MWCO, 0.5 mL, 10 cassettes 420 315

87730X4 15563227 4 x Slide-A-Lyzer G2 Dialysis Cassettes 10K MWCO, 3 mL, 10 cassettes 396 297

87731X4 15573227 4 x Slide-A-Lyzer G2 Dialysis Cassettes 10K MWCO, 15 mL, 8 cassettes 496 372

87732X4 15583227 4 x Slide-A-Lyzer G2 Dialysis Cassettes 10K MWCO, 30 mL, 6 cassettes 468 351

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1.3 Concentration devices

Thermo Scientific™ Pierce™ Protein Concentrators PES effectively combine simplicity, speed, capacity and recovery to yield enhanced concentration, purification and separation of proteins – even with dilute protein samples.

Premier disposable ultrafiltration centrifugal devices

Highlights:

Rapid protein concentration: sample concentration of 10 to 30-fold can be achieved in 10 minutes or less

Excellent protein recovery: unique design minimises membrane fouling and provides high speed concentrations, even with particle laden solutions

Instrument-compatible: use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning

Versatile: easy way to perform sample concentration, diafiltration or buffer- exchange of biological samples

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For more information visit thermofisher.com/proteinoffer

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Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

88512X4 15553237 4 x Pierce Protein Concentrators PES, 3K MWCO, 0.5 mL, 25 concentrators 388 291

88514X4 15573237 4 x Pierce Protein Concentrators PES, 3K MWCO, 2-6 mL, 10 reactions 168 126

88525X4 15513247 4 x Pierce Protein Concentrators PES, 3K MWCO, 5-20 mL, 10 reactions 340 255

88534X4 15553247 4 x Pierce Protein Concentrators PES, 5K MWCO, 20-100 mL, 4 reactions 392 294

88513X4 15563237 4 x Pierce Protein Concentrators PES, 10K MWCO, 0.5 mL, 25 concentrators 388 291

88516X4 15583237 4 x Pierce Protein Concentrators PES, 10K MWCO, 2-6 mL, 10 reactions 168 126

88527X4 15523247 4 x Pierce Protein Concentrators PES, 10K MWCO, 5-20 mL, 10 reactions 340 255

88535X4 15563247 4 x Pierce Protein Concentrators PES, 10K MWCO, 20-100 mL, 4 reactions 392 294

88502X4 15533237 4 x Pierce Protein Concentrators PES, 30K MWCO, 0.5 mL, 25 concentrators 388 291

88521X4 15593237 4 x Pierce Protein Concentrators PES, 30K MWCO, 2-6 mL, 10 reactions 168 126

88529X4 15533247 4 x Pierce Protein Concentrators PES, 30K MWCO, 5-20 mL, 10 reactions 340 255

88536X4 15573247 4 x Pierce Protein Concentrators PES, 30K MWCO, 20-100 mL, 4 reactions 392 294

88503X4 15543237 4 x Pierce Protein Concentrators PES, 100K MWCO, 0.5 mL, 25 concentrators 388 291

88523X4 15503247 4 x Pierce Protein Concentrators PES, 100K MWCO, 2-6 mL, 10 reactions 168 126

88532X4 15543247 4 x Pierce Protein Concentrators PES, 100K MWCO, 5-20 mL, 10 reactions 340 255

88537X4 15583247 4 x Pierce Protein Concentrators PES, 100K MWCO, 20-100 mL, 4 reactions 392 294

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1.4 Desalting devices

Available in five different spin column sizes, Thermo Scientific™ Zeba™ Desalting Devices provide trouble-free desalting, buffer exchange or unincorporated tag removal for sample volumes ranging from 2 mL to 4 mL.

The easy-to-use spin column format dramatically improves results over standard drip-column methodologies.

The perfect balance of speed and recovery

Highlights:

High recovery: low-binding resin maximises protein recovery

Fast: no fraction screening or waiting for protein to emerge by gravity flow

Easy to use: no cumbersome column preparation or equilibration

Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

89877X3 15523257 3 x Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75mL, 25 columns 303 202

89882X3 15533257 3 x Zeba™ Spin Desalting Columns, 7K MWCO, 0.5mL, 25 columns 267 178

89890X3 15543257 3 x Zeba Spin Desalting Columns, 7K MWCO, 2mL, 5 columns 495 330

89892X3 15553257 3 x Zeba Spin Desalting Columns, 7K MWCO, 5mL, 25 columns 618 412

89894X3 15563257 3 x Zeba Spin Desalting Columns, 7K MWCO, 10mL, 25 columns 750 500

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112We offer mixtures of proteins of known molecular weight (MW) or molecular mass (kDa) to assess the relative molecular sizes of proteins that have been separated by electrophoresis. The unstained, prestained and labelled molecular weight marker and ladder mixes exist for colourimetric, fluorescent and chemiluminescent methods.

2.1 Unstained protein ladders2.2 Prestained and PageRuler Protein Ladders2.3 Multicolour protein ladders2.4 Western blot chemiluminescent

and near infrared ladders2.5 Mini gel tank for protein

electrophoresis

2 Protein electrophoresis – protein ladders

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2.1 Thermo Scientific unstained protein ladders

Thermo Scientific™ Unstained Protein Ladders are a mixture of 8 to 14 proteins and peptides for use size standards that resolve into clearly identifiable sharp bands when analysed by SDS-PAGE.

Some proteins of the mix contain an integral Strep-tag™ II sequence and may be detected on western blots using Strep-Tactin™ conjugates.

For accurate protein molecular weight determination

Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

26632X4 15573197 4 x PageRuler™ Unstained Low Range Protein Ladder, 2 x 250 µL 236 177

26614X4 15523197 4 x PageRuler™ Unstained Protein Ladder, 2 x 250 µL 240 180

26630X4 15563197 4 x PageRuler™ Broad Range Unstained Protein Ladder, 2 x 250 µL 236 177

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For more information visit thermofisher.com/proteinoffer

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2.2 Thermo Scientific prestained and PageRuler protein ladders

Thermo Scientific™ Prestained Protein Ladders are recommended for monitoring the progress of the electrophoresis run and the efficiency of protein transfer to membranes during western blot procedures.

Thermo Scientific™ PageRuler™ Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins for use as size standards in SDS-PAGE and western blotting.

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder is a mixture of 9 blue-, orange-, and green-stained proteins for use as size standards in SDS-PAGE and western blotting.

The mobility of prestained proteins can vary in different SDS-PAGE buffer systems; however, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system.

For easy molecular weight determination and transfer efficiency

Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

26612X4 15513197 4 x Prestained Protein Molecular Weight Marker, 500 µL 176 132

26616X4 15533197 4 x PageRuler Prestained Protein Ladder, 10 to 180 kDa, 2 x 250 µL 404 303

26619X4 15543197 4 x PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa, 2 x 250 µL 416 312

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2.3 Thermo Scientific Spectra multicolour protein ladders

Thermo Scientific™ Spectra™ Multicolour Protein Ladders are three-to-four-colour protein standards containing prestained recombinant prokaryotic proteins from 10 to 300 kDa for use as size standards in gel electrophoresis and western blotting. The different chromophores (blue, orange, green and pink) are bound to the different component proteins, producing a brightly coloured ladder with an easy-to remember pattern.

For more information visit thermofisher.com/proteinoffer

A prestained, multicolour, SDS-PAGE molecular weight marker

Cat. no. Fisher Cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

26634X4 15583197 4 x Spectra™ Multicolor Broad Range Protein Ladder, 2 x 250 µL 508 381

26625X4 15553197 4 x Spectra™ Multicolor High Range Protein Ladder, 2 x 250 µL 612 459

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Cat. no. Fisher Cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

84786X4 15543227 4 x SuperSignal Enhanced Molecular Weight Protein Ladder, 250 µL 880 660

LC5602X4 15573267 4x MagicMark XP Western Protein Standard, 250 µL 784 588

26635X4 15593197 4 x PageRuler Prestained NIR Protein Ladder, 2 x 250 µL 752 564

2.4 Thermo Scientific molecular weight protein ladders for western blotting

The Thermo Scientific™ SuperSignal™ Molecular Weight Protein Ladder (20 to 150 kDa) and the Thermo Scientific™ MagicMark™ XP Western Protein Standard (20 to 22kDa) are a ready-to-use mixture of eight and nine recombinant proteins with IgG-binding sites that provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent, fluorescent, chromogenic or other detection systems.

The Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250 kDa) that are blue-stained and fluor-labeled for near-infrared (NIR) fluorescent visualisation and protein sizing following electrophoresis.

Estimate protein molecular weight alongside your western blot results

Requires fluorescent imager capable of NIR fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR™ Odyssey™ Infrared Imaging System.

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2.5 Mini gel tank for protein electrophoresis

The Mini Gel Tank is designed for more intuitive use and greater convenience compared to traditional electrophoresis tanks. The unique, side-by-side tank design allows you to perform electrophoresis of 1 or 2 mini gels.

One tank, more than 200 gels

FREEGELTANK

Highlights:

Versatile: compatible with all of our mini gels, including InvitrogenTM NuPAGETM, NovexTM, BoltTM, and specialty gels

Easy sample loading: forward-facing well configuration

Simultaneous visualisation of both gels: streamlined, side-by-side tank configuration

Simple monitoring of gels: white tank stand provides easy visualisation of prestained markers

Less running buffer required: gel chambers are separated, so you only need to load sufficient buffer for each gel to the specified fill line

Need a new gel tank?

Get one for free with only five boxes of precast gels

For details of qualifying products and to claim your free gel tank, please visit: thermofisher.com/proteinoffer

product number A25977

Please quote promo code R5GEL1 when ordering from Thermo Fisher Scientific

Please quote promo code R5GEL4 when ordering from Fisher Scientific

1733. Western blotting apparatus and reagents

Western blotting can be easier and faster when you use our full line of products during your experiment. You can rely on our Thermo Scientific™ protein biology products, especially during the last few critical steps.

3.1 Dry transfer apparatus and reagents for western blot

3.2 Semi automated apparatus and reagents for western immunoblotting

3.3 Chemiluminescent reagents3.4 X-ray films for chemiluminescence3.5 Western blot stripping buffers

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3.1 Dry transfer apparatus and reagents for western blot

The Invitrogen™ iBlot™ 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers. The iBlot 2 Gel Transfer Device is an integral part of the Invitrogen™ iBlot™ 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

Highlights:

Fast: transfer proteins in 7 minutes

Optimised reagents: high-quality premade transfer stacks for reproducible results

Reliable transfer efficiency: transfer low, medium and high MW proteins (10 to 300 kDa) equivalent to or better than conventional semi-dry or wet transfer methods

Easy-touch programming: access pre-programmed transfer methods or easily create, run and save custom transfer methods

Cat. no. Fisher Cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

IB23002X3 15543267 3x iBlot™ 2 Transfer Stacks, nitrocellulose, mini, 10 stacks 306 204

IB23001X3 15533267 3x iBlot 2 Transfer Stacks, nitrocellulose, regular size, 10 stacks 372 248

IB24002X3 15563267 3x iBlot 2 Transfer Stacks, PVDF, mini, 10 stacks 345 230

IB24001X3 15553267 3x iBlot 2 Transfer Stacks, PVDF, regular size, 10 stacks 417 278

Cat. no. Fisher Cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

IB21001PR 15502897 iBlot 2 Gel Transfer Device - 30% off list price 1308 915.60

Rapid and efficient protein transfer at your fingertips

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3.2 Semi automated apparatus and reagents for western immunoblotting

The Invitrogen™ iBind™ Flex Western System offers flexible blot processing to optimise antibody use, easily change formats, and reduce hands-on time. Simply load primary and secondary antibody solutions and wash solutions into the device, then walk away. In less than 3 hours, the blot is ready for final detection.

Flexible formats – less antibody – reproducible results

Highlights:

Flexibility — process up to one midi blot, two mini blots or six vertically cut strips using the same or different conditions

Compatibility — use nitrocellulose or PVDF membranes, directly labelled primary or secondary antibody detection (AP, HRP or fluorescent labelled)

Cost savings — use up to 80% less primary antibody than with traditional tray-based incubation steps for western blotting

Cat. no. Fisher cat. no. DescriptionEveryday low price

GBP (£)

SLF2000S 15307238iBind™ Flex Western Device Starter Kit - 40% savings when purchased as a bundleIncluding: 1 iBind Flex Western Device, 10 iBind Flex Cards, 1 iBind Flex Solution Kit

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Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

SLF2010X3 15583267 3x iBind™ Flex Cards, 10 cards 501 334

SLF2020X3 15522897 3x iBind™ Flex Solution Kit, 1 kit 234 156

SLF2019X3 15512897 3x iBind™ Flex Fluorescent Detection (FD) Solution Kit, 1 kit 281.25 187.50

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3.3 Chemiluminescent reagents

Thermo Scientific™ SuperSignal™ Chemiluminescent HRP Substrates and Thermo Scientific™ Pierce™ ECL Chemiluminescent HRP Substrates offer a broad range of sensitivity and signal duration for your western blotting applications.Thermo Scientific™ Pierce™ ECL Western Blotting Substrate is an entry-level western blotting substrate. SuperSignal Chemiluminescent HRP Substrates offer excellent performance.

For louder and clearer signals

Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

32209X4 15513207 4 x Pierce™ ECL Western Blotting Substrate, 250 mL 508 381

32106X4 15503207 4 x Pierce ECL Western Blotting Substrate, 500 mL 668 501

34580X4 15503217 4 x SuperSignal™ West PICO Plus Chemiluminescent Substrate, 500 mL 540 405

34578X4 15593207 4 x SuperSignal West PICO Plus Chemiluminescent Substrate, 1 L 1000 750

34075X4 15523207 4 x SuperSignal™ West DURA Extended Duration Substrate, 100 mL 1292 969

34076X4 15533207 4 x SuperSignal West DURA Extended Duration Substrate, 200 mL 2072 1554

34095X4 15573207 4 x SuperSignal™ West FEMTO Max. Sensitivity Substrate, 100 mL 1652 1239

34096X4 15583207 4 x SuperSignal West FEMTO Max. Sensitivity Substrate, 200 mL 2672 2004

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Highlights:

Perfect match: a broad range from regular to the most sensitive substrate matching any requirements

Affordable: costs up to 50% less than other brands of comparable chemiluminescent substrates

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3.4 X-ray films for chemiluminescence

Thermo Scientific™ CL-XPosure™ X-ray Films are high-performance, single-emulsion, clear-blue films. This film works with chemiluminescent reagents for either alkaline phosphatase (AP) or horseradish peroxidise (HRP) and can also detect radioactivity, making it ideal for western, Southern and northern blots, and electrophoretic mobility shift assays (EMSA).

For razor-sharp bands

Highlights:

High performance: just as sensitive as other commercially available autoradiography films

Convenient: available in different sheet sizes

Perfect match: validated for use with SuperSignal Chemiluminescent Substrates and Pierce ECL Chemiluminescent Substrate

Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

34089X3 15543207 3 x CL-XPosure Film (18 x 24 cm), 100 sheets 465 310

34090X3 15553207 3 x CL-XPosure Film (13 x 18 cm), 100 sheets 357 238

34091X3 15563207 3 x CL-XPosure Film (20 x 25 cm), 100 sheets 480 320

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3.5 Western blot stripping buffers

Thermo Scientific™ Restore™ Western Blot Stripping Buffers are easy and ready to use, making them ideal for ‘everyday’ western blots.

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer is a unique formulation that efficiently removes high affinity antibodies and reduces incubation times at room temperature.

Strip time off your research safely and effectively

Highlights:

Saves time: no need to re-run gels and blots

Saves costly sample: reprobe the membrane using the same target sample

Gentle: does not damage the target antigen during stripping allowing efficient reprobing

Odour-free: no mercaptans means no acrid odours

Cat. no. Fisher cat. no. DescriptionList priceGBP (£)

Promo priceGBP (£)

21059X4 15503197 4 x Restore Western Blot Stripping Reagent, 500 mL 504 378

46430X4 15513217 4 x Restore Plus Western Blot Stripping Buffer, 500 mL 540 405

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