Diploma in Clinical Pathology - Ain Shams...

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Diploma in Clinical Pathology

الاثوولوجاث اكاللاياياةدبلوم يفدرجة ال

Program Code: CP900

Program Guide and

Logbook

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C a n d i d a t e C u r r i c u l u m v i t a e

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TABLE OF CONTENTS: Page

I – Welcome Statement

II - Mission Statement

III – Senior supervisor and affiliated departments and Hospitals

IV – Program specifications

1 - Basic Information

2 - Professional Information

3 – Academic standards

4 – Curriculum structure and contents

5 – Program courses:

6. Program admission requirements:

7. Regulation for progression and program completion

8. Appendix of course specifications

- Basic clinical chemistry1 course specifications

- Basic laboratory hematology1 course specifications

- Basic clinical immunology1 course specifications

- Basic clinical microbiology1 course specifications

- Clinical chemistry2 course specifications

- Laboratory hematology2 course specifications

- Clinical immunology2 course specifications

- Clinical microbiology2 course specifications

-Elective courses:

- Total quality management in clinical laboratory course specifications

- Molecular diagnostics course specifications.

- Clinical applied immunology course specifications.

- Medical research course specifications.

- Therapeutic drug monitoring and clinical toxicology course specifications.

- Infection control course specifications

- Blood transfusion course specifications

a-Basic information

b-Professional information

V-General information

VI-Your log book

1 – Introduction

2 – Supervisors

3 - Tables for training records

4 – Log book preview

VII - Head of department approval for the exam entry

Detachable forms:

VIII – Thesis follow up

IX - Evaluation forms

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I – WELCOME STATEMENT:

The Department of Clinical Pathology welcomes you to the Diploma degree in

Clinical Pathology. As a department we are committed to medical student

education and continuously strive to improve their educational experience.

This handbook presents an information guide and logbook activity of the Diploma

degree in Clinical Pathology administered by the Clinical Pathology Department,

Faculty of Medicine, Ain Shams University.

II - MISSION STATEMENT:

The mission of the Faculty of Medicine, Ain Shams University is “The

preparation of a competent graduate, who is able to compete on both national

and regional levels, capable of lifelong learning, training and tutoring, while

adhering to the codes of practice of medical health services and ethics. The

college as well, seeks continuous development of programs and courses. It also

enhances expansion of applied scientific research and health programs for

community services and environmental development. Moreover, through

providing distinguished academic and research cadres of teaching staff,

supporting the administrative system and sustainability of own resources, the

college is able to achieve goals and objectives”.

The mission of the clinical pathology department is to develop clinical

pathologists who are dedicated to the betterment of society. This will be achieved

by focusing on continuous improvement of knowledge, a commitment to

excellence in learning and gathering of information and enhancement of laboratory

skills implemented in a safe environment.

III – SENIOR SUPERVISOR AND AFFILIATED

DEPARTMENTS AND HOSPITALS

SENIOR SUPERVISOR

Prof.

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-------------------------------

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E-mail:

AFFILIATED DEPARTMENTS AND HOSPITALS

Hospital of Nasser Institute

8

Military Academy

Police hospital

Hospitals of other universities

Nasr city hospital of medical insurance

IV – PROGRAM SPECIFICATIONS

A- Basic Information

1. Program title:

Diploma in Clinical Pathology

2. Program type: Single

3. Faculty

Faculty of Medicine

4. Department

Clinical Pathology Department

5. Assistant co-ordinator

………………………………………………

6. Co-ordinator

………………………………………………

7. Last date of program approval:

……………………………………………………………

B- Professional Information:

1. Program aims:

By the end of the program of diploma degree, the student will be a trained

specialist in clinical pathology, equally qualified in clinical chemistry, hematology,

immunology and microbiology, with the aim of efficiently managing a clinical

laboratory

2. Intended learning outcomes (ILOs):

a. Knowledge and understanding:

By the end of the program the candidate will be able to:

a1- Recognize different techniques and instruments used in the laboratory.

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a2- Recognize clinical application and reference values of different tests performed

in the laboratory.

a3- Recall different methods of assay for routine laboratory tests.

a4- Discuss different aspects of automation.

a5- Recognize the patho-physiology of different diseases and organ dysfunctions

and the appropriate tests used for the diagnosis and follow up of each.

b. Intellectual capabilities:


b1- Interpret the results of different tests

b2- Apply quality control measures

b3 - Apply infection control and safety measures in the laboratory.

b4- Select tests appropriate for diagnosis, prognosis, follow up and monitoring of

treatment for common diseases and malignancies.

b5- Establish laboratories at different service levels.

b6- Design and construct scientific research work through data collection,

performance of new tests, statistical work to analyze and present data in the form

of thesis presentation.

c. Professional and practical skills:


c1- Use basic laboratory instruments to perform efficiently routine and some

research parameters.

c2- Manage with fair quality a clinical pathology laboratory with its different

specialties.

c3- Master the techniques of specimen collection, handling and processing.

d. General and transferable skills:


d1 - Think creatively and innovatively, with individual initiative and ability to

work in a team.

d2 - Undergo fruitful laboratory- physician interaction to ensure correct decision

making.

d3 - Undergo proper problem-based learning and IT application for maximum

knowledge collection and case presentation.

3. Academic standards: (Benchmarks)

The curriculum of the Royal College of Clinical Pathology

4. Curriculum structure and contents:

4a- Program duration:

Diploma: 2years (four semesters)

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4b- Program structure:

انمنبهح

انسبعبث انكود انمقرراث انذراسيت

انمعتمذة

ورة أسبسيبث انبحث انعهميد متطهببث انكهيت

CP9001 5.1 اسبسيبث انكيميبء االكهينيكيت اندزء األول

CP9002 5.1 اسبسيبث امراض انذو انمعمهيت

CP9003 5.1 اسبسيبث انمنبعت االكهينيكيت

CP9004 5.1 اسبسيبث انميكروبيونوخي االكهينيكيي

CP9005 1.1 انكيميبء االكهينيكيت اندزء انثبني

CP9006 1.1 امراض انذو انمعمهيت

CP9007 3 انمنبعت االكهينيكيت

CP9008 3 انميكروبيونوخي االكهينيكيي

يختبر انطبنب مبدة واحذة من : االختيبريت انمقرراث

E9118-ادارة اندودة في انمعبمم

-انتشخيص بأستخذاو انبيونوخيب اندزيئيت

E9002 تطبيقيت انمنبعت االكهينيكيت ان-

E9015 انبحث انعهمي-E9121 معبيرة

مكبفحت انعذوى - E9122-انعالج انذوائي

-E9059 نقم انذو انعالخي-E9124

E9118

E9002

E9015

E9121

E9122

E9059 E9124

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6 كراست األنشطت

33 انمدموع

5. Program courses:

a. Compulsory courses:

Code Course title No of hours (Actual)

L P F SDL CP9001 Basic clinical chemistry 1 course specifications 16 14

CP9002 Basic laboratory hematology 1 course specifications 16 14

CP9003 Basic clinical immunology 1 course specifications 16 14

CP9004 Basic clinical microbiology 1 course specifications 16 14

CP9005 Clinical chemistry 2 course specifications 60 45

CP9006 Laboratory hematology 2 course specifications 60 45

CP9007 Clinical immunology 2 course specifications 30 30

CP008 Clinical microbiology 2 course specifications 30 30

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L: Lecture, C: Clinical, F: field and SDL: Self directed learning

b. Elective courses

Code Course title

L C F SDL E9118 Total quality management in clinical laboratory course

specifications

15

E9002 Molecular diagnostics course specifications. 15 E9015 Clinical applied immunology course specifications. 15 E9121 Medical research course specifications. 15 E9122 Therapeutic drug monitoring and clinical toxicology course

specifications.

15

E9059 Infection control course specifications 15 E9124 Blood transfusion course specifications 15

L: Lecture, C: Clinical, F: field and SDL: Self directed learning

6. Program admission requirements:

-انمبخستير: ( : يشترط نقيذ انطبنب نهحصول عهي درخت6) انمبدة

ػ دزجت ابىازيض في اطب اجسادت إدد جاؼاث جزيت صس أ يى داصلا - 1

-04افمت جت اؼ -03أ يى لد أض ظت اخدزيب )االخياش( -2 0اؼسبيت أ ػ دزجت ؼادت

-05( 1اطبت في اذك )حعديد اسظ صازيف اخدزيب اظخلن األجصة اظخيفاء اعخداث

0اخفسؽ دزاظت دة فصي دزاظيي لب دخي اخذا اجصء اثاي

-ااجعخيس األزاق اخايت :ت يمد طاب اإلخذاق دزج ( :5مهحق )

شهادة اهيلد أ عهخ سس زظهي -4 0شهادة اإلخيهاش -3 0شادة ابىازيض - 2 0طب إخذاق -1

صههز 6ػههدد - 7 0افمههت جههت اؼهه ػهه اخعههجي اخفههسؽ اطهه - 6 0لههف هه اخجيههدا - 5 0

باعههبت افههدي يمههد اطاههب افمههت اعههفازة يذههدد جههت حذهه افمههاث وهها يمههد - 8 0فحغسافيههت جديههدة

0شادة صذيت

7. Regulation for progression and program completion

يخ اخعجي دب اؼا سة اددة في اعت حبدأ أي يي دخ أخس أغعطط ػه أ حبهدأ (: 8مبدة )

فهي افخهسة ه أي ؼيهدي اافهدي اها ايجهش لبهي حعهجي 0ادزاظت في شس أوخبس وه ػها

إمضههاء فخههسة ادزاظههت خذهها اجههصء األي إال بؼههد إههفبسدخ أخههس ديعههبس ػهه أال يعههخ هه بههدخي

خعهجي هدزجاث حبدأ ادزاظت ف إبسي غ ساػاة حظهي فصه دزاظه صهيفي ه بأها باعهبت اطبت

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سحي ف اؼها : األه ه أي ييه دخه غخهس أغعهطط اثايهت ه أي دباث فيخ ادوخزا بال ا

0فبس دخ غخس ديعبس

زاظت ف و ػا جاؼ ػ فصي دزاظيي دة وه ها خعهت ػاهس اظهبػاا ب يبهدا حشع اد(: 9مبدة )

االي ف أي أوخبس يبدا اثا ف صف فبسايسب غ حظي فص دزاظ صيف ىثف دة ظهخت اظهابيغ ب

امهسزاث ادزاظه لبه اظهبػي ه بدايخه ػه االله بؼهد إظهخيفاء ااهسط دعهب يهخ اخعهجي فصه

ظهاػاث ؼخهدةب يجهش طاهب حؼهدي 6اعجتب اليبغ أ يصيد اؼبء ادزاظ ف افص اادد ػه

امسزاث خلي اظبػي بدايت افص ادزاظه ) باذهرا ا االفهافت (ب وها يجهش ه االعهذا خهلي

ظخ اظابيغ ادد امسازث د ادخعاب زاظباا فيب

: دة ادزاظت ذصي ػ دزجت ادب أزبؼت ػاس شهساا )أزبؼهت فصهي دزاظهيت ( ( 55بدة )انم

ػهه جههصيي يجخههاش فيهها اطاههب بساجههاا حههدزيبياا خىههالا طبمهها عههاػاث اؼخههدة افههذت بابهها اسابههغ

ي اإلخذهها لبهه إظههخيفاء ل ههت ال يعههخ هه بههدخاألاههطت يعههخف خلهها اطهه ههت فهه وخيههب خابؼههت

0أزباع اط ت في و جصء ابساج

يجخههاش بؼههدا اطاههب إخذاههاا ال ادههد ههدة ادزاظههت فههي اههدب اجههصء األي فصهه دزاظههي ( :51انماابدة )

خهدزيبطاهب ياخسط اجاح في باىاه نخمهاي دزاظهت فهي اجهصء اثهايب اجهصء اثهاي يخطهب حفهسؽ ا

ببأدد اعخافياث أ اساوص اؼخدة اىيت دة فصي دزاظيي

يجش ذاصي ػ دزجت ادب اؼا إظخىاي دزجهت ااجعهخيس بخعهجي زظهات ااجعهخيس (: 51مبدة )

جاؼههت خههلي أزبههغ ظههاث هه اذصههي ػهه دزجههت اههدب يذصهه ػهه ادزجههت بؼههد إظههخيفاء خطبههاث ا

اىيت الات اسظات بجاحب

اعاػاث اؼخدة دزاظت ادب ااجعخيس ل ظاػت ؼخدة ػ األل ي صص ا ظهج (: 56مبدة )

حا ظج ظاػاث ػه األله جهصء األي هاي ػاهسة ظهاػت ػه األله خابؼت األاطت ظاػاث ىخيب

جصء اثايب

باميد دزجت ادب اؼا أزبغ ظاثدة (: 11مبدة )

فهغ إخذاهاث فهػيت حاه اللعها اؼيهت باألشهخسان هغ ألعها اهاد اسحبطهت بخص اح(: 13مبدة )

ظههاي اخميههي ا خفههت هه أظههوت طيههت لصههيسة خؼههددة اإلخخيههازاث ك إخخبههازاث إوييىيههت مههت حمههيط

حا وساظت ااج حفاصي ذه حؼخد جت ادزاظاث اؼيا باىيت ب اازاث ا خفت ػ أ

دزجههت جههصء األيب 300دزجههت هها 1200جههع دزجههاث االخذهها اههاي ههدب اؼهها (: 12ماابدة )

.دب اؼادزجت 300 اخساو با ياش صيضاا ايا اؼدي اف

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ادز األي في أوخبس فبس ه وه ػها يؼمهد إخذها اهدز اثهاي فه أبسيه يؼمد إخذا(: 11مبدة )

اي و ػاب

% ادزجت اىيت ى جها 60يى اجاح ف و ادة ادب اؼا بؼد اذصي ػ (: 16مبدة )

% 50 اال يم اخذسيس ػ ػباادة جخؼت

Assessment Schedule and Weighing of Assessments

Item Mark

Po

ints

GP

A

sco

re

بطا اتدا

Stu

den

t

sta

te

During

semester

End of

semester Total

First semester (If present) 100 300 400

Second semester 100 100

Third semester 100 100

Fourth

semester

Final exam

Written

900 900

Oral

Practical

/Clinical

Total 300 1200 1500

ػ اج اخا:ذظت: حؼادي دزجاث اطاب طبما ماط

نقبط 2 : % فأكثر 93 -5 A

نقبط 3.63 : % 93% حتي أقم من 81من -1 A-

نقبط 3.33 : %81% حتي أقم من 83من - 3 B+

نقبط 3.33 : %83% حتي أقم من 31من - 2 B

نقبط 1.63 : %31% حتي أقم من 33من - 1 B-

نقبط 1.33 : %33% حتي أقم من 61من - 6 C+

نقبط 1.33 : %61% حتي أقم من 61من -3 C

نقبط 5.63 : %61% حتي أقم من 63من - 8 C-

F صفر : %63أقم من - 9

دزجت جصء 300دزجت ا 1200جع دزجاث االخذا ااي دب اؼا ااجعخيس

دزجت جصء األي إ جدب 300دزجت ا 1500األي جع دزجاث االخذا ااي دوخزا

دزجت 400دزجت دب اؼا ااجعخيس 300اخساو با ياش صيضاا إيا اؼدي اف

.دوخزا

( ػ أظاض جع داص فس ماط و مسز ضسباا فه GPAيخ دعا اؼدي افص )

ػدد ظاػاح اؼخدة معاا ػ اعاػاث اؼخدة مسزاث اخ دزظا اطاهب فه افصه ادزاظهب وها

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( ػ أظاض جع داص فهس امهاط اخه دصه ػيها CGPAاؼدي اخساو طاب ) دعا يخ

اطاب ف و مسز ضسباا ف ػدد ظاػاح اؼخدة معاا ػ جع اعاػاث اؼخدة اىيتب

يخ اإلػهادة أ ادوخزا ااجعخيس ف ادب أمسزاث اف دات اسظ ف ادة أ جػت

يهخ دعها اخمهديس افؼه اهر يذصه ػيه فه أي إػهادة فمهظ أها إذا حىهسز ف اادة أ اجػهت فمهظب

ماط ا 67ب1% فمظ )ا60زظبت فيذعب ػد اجاح حمديس -

C).

Basic clinical chemistry1 course specification: University: Ain Shams Faculty of Medicine

Course specifications

Program on which the course is given: Diploma in Clinical Pathology

Major elements of program: Major

Department offering the program: Clinical Pathology Department

Department offering the course: Clinical Pathology Department

Academic year / Level Diploma – 1st semester

Date of specification approval ………………………………………..

Basic Information

Title: Basic Clinical Chemistry 1 Code: CP9001

Credit Hours: 1.5 hour Lecture: 1/week Total: 16

Practical: 1/week Total: 14

Coordinator

Head of Clinical Chemistry Unit (Prof. Hanzada I.A. Fattah)

B - Professional Information

1- Course Aims:

By the end of the course the candidate will have been introduced to the clinical

chemistry laboratory and will be able to:

a- Appreciate the basic concepts related to the clinical chemistry laboratory.

b- Recognize the principles of different techniques and procedures applicable to

the clinical laboratory.

2- Intended Learning Outcomes (ILOs) from the Course:

a- Knowledge and understanding

By the end of the course the candidate will be able to:

a1 - Recognize the basic concepts used in spectrophotometry.

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a2-Identify the different pre-analytical variables with sampling and sample

processing.

a3-Appreciate the principles of laboratory safety.

a4- Recognize the principles of laboratory analyses including the concept of

standards, reference materials, units of measurement, basic techniques and

procedures.

a5-Identify the basic concepts of medically applied statistics.

b- Intellectual skills


b1- Deal efficiently with spectrophotometers

b2 - Direct physicians to optimum sampling conditions and patient preparation.

b3 – Implement safety procedures.

b4 – Deal with appropriate reference materials and standards

b5- Apply basic statistical procedures

c- Professional skills


c1- Efficiently operate, evaluate and maintain spectrophotometers.

c2- Master the technique of sample collection, handling and processing.

c3-Apply laboratory safety procedures.

c4- Prepare standards and acids.

c5- Use statistical methods in the laboratory.

d- General and transferable skills

By the end of the course the candidate will be able to: d1-Become familiar with the work environment in the laboratory.

d2 -Undergo fruitful interaction with technicians and fellow doctors.

d3 –Become acquainted with IT applications for maximum knowledge collection.

d4 - Implement safe laboratory procedures.

e- Attitude


e1- Establish close rapport and understanding with laboratory staff, users of the

laboratory service and service managers.

e2- Appreciate integral importance of teamwork in running a diagnostic laboratory

service

3- Course content:

Topics No. of hours

L T C/P SDL

Instrumentation ( basic) 5

Introduction to laboratory analysis 2

Laboratory safety 2

Sampling and pre-analytical variables 2

Introduction to medical statistics 5

16

Spectrophotometer 2

Centrifuge, glassware, pipette 2

Balance and weights 2

Acid preparation 2

Calculations 4

Sampling 2

L: Lecture, T: Tutorial, C/P: Clinical or Practical and SDL: Self directed learning

4 - Student Assessment Methods

4.1 written exam to assess knowledge and intellectual skills

4.2 oral exam to assess knowledge, attitude and communication skills

4.3 during-semester evaluation to assess practical abilities.

Weighing of assessment

4.1 written exam 60

4.2 oral exam 15

4.3 during-semester 25

Total 100

5 - List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

1-Teitz Textbook of Clinical Chemistry and Molecular Diagnostics,4th edition,

Elsevier Saunders, Westline Industrial Drive St. Louis Missouri,2007

2- Clinical Chemistry From Principles to Practice. 2nd Edition.2008

5.3- Recommended Books:

Clinical Chemistry: Principles, procedures, correlations, 4th

edition. Michael

L.Bishop, Janet L.Duben-Engelkirk and Edward P. Fody

5.4- Periodicals, Web Sites, etc

medline.com, cpainshams.com

Journals: Journal of Clinical chemistry, (available in the library of Ain Shams

Specialized Hospital)

BASIC Laboratory HEMATOLOGY 1 COURSE SPECIFICATIONS University: Ain Shams Faculty of Medicine






Academic year/Level Diploma – 1st semester

Date of specification approval ……………………………………….

17


Title: Basic Hematology 1 Code: CP9002

Credit hours: 1.5 hours Lecture: 1/week Total: 16

Practical: 1/week Total: 14

Coordinator

Head of Laboratory Hematology Unit


1- Course Aims


a- Appreciate the basic concepts related to the lab hematology and transfusion

medicine


the hematology lab and transfusion medicine

2- Intended Learning Outcomes (ILOs) from course



a1- Understand the physiology of hematopoiesis, normal hemostasis and the

principles of transfusion medicine.

a2- Recognize the basic concepts of molecular biology and cytogenetics and flow

cytometry

a3- Identify the different pre-analytical variables with sampling and sample

processing.

a4- Recognize the principles, limitations and pitfalls of laboratory analyses

including hemoglobinometry, manual blood count, differential count, reticulocyte

count and screening coagulation tests.

a5- Demonstrate knowledge of the blood donation process, principles of

component preparation, appropriate use of blood, pre-transfusion testing and

complications of blood transfusions

a6- Appreciate the principles of laboratory safety



b1- Direct physicians to optimum sampling conditions and patient preparation

b2- interpret the hematological test results in relation to normal values

b3- Assess transfusion therapy risk/benefit balance, recommend appropriate blood

or component therapy and resolve logistic problems inherent in meeting demands

for blood and components under routine and emergency conditions.



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c1- Practice safe laboratory work and apply principles of universal precautions

c2- Master the technique of sample collection, handling and processing.

c3- Efficiently perform manual CBC (including identification and evaluation of

morphology of normal cellular elements), reticulocyte count, screening coagulation

tests.

c4- Skillfully perform ABO and Rh blood grouping and compatibility testing

recognizing technical limitations and pitfalls.



d1- Become familiar with the work environment in the laboratory

d2 -Undergo fruitful interaction with technicians and fellow doctors

d3 –Become acquainted with IT applications for maximum knowledge collection

d4 – Release results and communicate information in an understandable manner

e- Attitude





service

3- Course content:

Topics No. of hours

L T C/P SDL

Hematopoiesis 4

Lymphocyte development 1

Physiology and evaluation of hemostasis 4

Introduction to cytogenetics 1

Introduction to molecular biology 2

Principles of blood transfusion medicine 4

Sampling, anticoagulants, Concept of normal

values, Critical values in hematology

1

Hemoglobinometry 2

Hemocytometer, manual total WBC count,

manual platelet count

3

How to prepare and stain a blood film, normal

differential count, absolute values

3

ESR, Hct, RBC indices, reticulocyte count 1

Bleeding time, PT, PTT, TT 2

Blood grouping and cross matching 2



4.1 Written exam to assess knowledge and intellectual skills

19

4.2 Oral exam to assess knowledge, attitude and communication skills

4.3 During-semester evaluation to assess practical abilities


4.1 Written exam 60

4.2 Oral exam 15

4.3 During-semester 25

Total 100


5.1- Course Notes (paper and/or electronic)

5.2- Essential Books (Textbooks)

1- Essential Hematology, last edition

2- Dacie Practical Hematology, last edition

5.3- Recommended Books

1- Postgraduate Hematology, last edition

5.4- Websites and periodicals

1- medline.com,

2- cpainshams.com

3- Blood (journal available at Ain Shams University Hospital Library)

Basic Clinical Immunology1 Course specifications: University: Ain Shams Faculty of Medicine









Title: Basic Clinical Immunology1 Code: CP9003

Credit Hours: 1.5 hour Lecture: 2/every other week Total: 16

Practical: 2/ every other week Total: 14

Coordinator

Head of Clinical Immunology Unit (Prof. Mona Rafik)


i- Course Aims:

To acquire thorough knowledge of fundamental immunology.

20

ii- Intended Learning Outcomes (ILOs) from the Course:



a1 - Understand the fundamental mechanisms and the basic concepts required for

maintenance of the integrity of the immune response.

a2 - Acquire a core body of knowledge in fundamental immunology to underpin

clinical and laboratory practice



b1-Integrate knowledge of fundamental immunology to understand the patho-

physiology of immune mediated diseases.



c1- Acquire and be able to apply a solid foundation of knowledge of the various

immunological processes required to maintain a normal effective immune

response.


By the end of the course the candidate will be able to

d1-Be aware of relevant sources of information including computerized databases

and should have the skills to use information resources to keep up to date with the

latest developments in this rapidly developing field.

e- Attitude


e1- Recognize vital importance of fundamental immunology to practice as a

clinical immunologist

e2-Have the requisite skills to maintain their Continuing Professional

Development.

e3 - Adhere to professional and ethical conduct

3- Course content:

Topics No. of hours

L T C/P SDL

Principles of body defense

The acute phase response and

inflammation

Anatomy of the immune system

Complement

1

1

1

1

21

Phagocytosis,

Natural or innate immune response

1

1

The basis of specific immunity

Antigens and antigen processing and

presentation

Major Histocompatibility Complexes:

T Lymphocytes and cell mediated

immunity

B Lymphocytes and humoral immunity

Cytokines, chemokines and

immunomodulators

2

1

2

2

1

Immunoregulation

Mucosal compartments of the lymphoid

system

1

1

1.Sampling and preanalytical precautions for

immunological samples

2.Gel Immunoprecipitation, and

immunodiffusion simple and electrodiffusion,

single or double diffusion in one or two

dimensions / phases e.g. radial immunodiffusion

and Counter current Immunoelectrophoresis

Detection and quantification of

Cryoglobulins

Detection and quantification of other

relevant proteins and molecules

Quantification of Immunoglobulins

i.e. IgG, IgA, IgM, IgD, IgG subclasses ,lgE) in

serum and other body fluids e.g. urine & CSF

Methods for measuring specific IgG

and IgE antibodies i.e. functional antibodies

(tetanus, H influenzae and Pneumococcus),

precipitins (avian, aspergillus), anti-venom and

other allergen specific antibodies.

3.Fluid phase Immunoprecipitation

1

1

1

1

1

1

1

1

22

4.Agglutination techniques

5.Investigation of Complement activation

6.C3 nephritic factor

7.Assessment of Neutrophil function:

8.Protein Electrophoretic separation

2

1

2

1


4 -Student Assessment Methods

4.1 written exams to assess knowledge and intellectual skills

4.2 oral exams to assess knowledge, attitude and communication skills

4.3 During-semester evaluation to assess practical abilities


4.1 written exam 60

4.2 oral exam 15


Total 100


5.1- Course Notes

LECTURES


Immunology. Roitt , Ivan M. (ed), Jonathan Brostoff (ed), David K. Male (ed).

Mosby, Inc


a- http://www.med.sc.edu:85/book/immunol-sta.htm - (Teacher Information,

Student Help, Graphics) Immunology online textbook; 18 chapters cover entire

immunology spectrum. Graphics support text beautifully.

b- Kuby Immunology. Richard A. Goldsby, Thomas J. Kindt, Barbara A.

Osborne, Jani Kuby. W H Freeman


http://www.bio.davidson.edu/courses/immunology/Bio307.html#anchor24514174

- (Graphics) Descriptions of aspects of immunity with fantastic graphics (and

sound effects!) to accompany. Covers MHC antigen loading, second messengers, B

cell maturation/activation, B/T cell selection, etc.

http://www.biology.arizona.edu/immunology/tutorials/immunology/main.html -

(Student Help) Variety of immunology questions with multiple choice answers.

Correct answers are reinforced with a brief explanation. Incorrect answers are

linked to tutorials to help solve the problem.

http://gsbs.utmb.edu/microbook/ch001a.htm - Immunology overview; description

of immune system components and systems, evolution of immune system,

http://www.med.sc.edu:85/book/immunol-sta.htm

http://www.bio.davidson.edu/courses/immunology/Bio307.html#anchor24514174

http://www.biology.arizona.edu/immunology/tutorials/immunology/main.html

http://gsbs.utmb.edu/microbook/ch001a.htm

23

functions of components, mechanisms Pictures very helpful. Text somewhat more

advanced.

http://www-micro.msb.le.ac.uk/MBChB/ImmGloss.html - (Teacher Information,

Student Reference) Dictionary of common immunology terms with definitions

http://www.rheumatology.org.nz/nz16000.htm - (Teacher Information, Student

Reference) Immunology dictionary

Basic Clinical Microbiology 1 Course Specification University: Ain Shams Faculty of Medicine








A-Basic Information

Title: Basic Clinical Microbiology1 Code: CP9004



Co-ordinator

Head of Clinical Microbiology Unit


i- Course Aims:

By the end of the course the candidate will have been introduced to the clinical

microbiology laboratory and will be able to:

a- Appreciate the basic concepts related to the clinical microbiology laboratory.


the clinical laboratory.




a1- Recognize different types of microorganisms.

a2- Identify the different methods of sterilization and disinfection.

a3- Appreciate the principles of laboratory safety.

a4- Define different parasites of medical importance.

http://www-micro.msb.le.ac.uk/MBChB/ImmGloss.html

http://www.rheumatology.org.nz/nz16000.htm

24



b1- Understand different sterilization principles.

b2 - Implement safety procedures.

b3- Interpret different organisms on different culture media



c1- Master the handling and maintenance of microscopy.

c2- Perform efficiently preparation of wet and stained films.

c3-Apply laboratory safety procedures.


By the end of the course the candidate will be able to: d1- Become familiar with the work environment in the laboratory.

d2- Undergo fruitful interaction with technicians and fellow doctors.

d3- Implement safe laboratory procedures.

e- Attitude





service

C- Course content:

Topics No. of hours

L T C/P SDL

Gram positive cocci & bacilli 2

Gram negative cocci & bacilli 2

Acid fast bacilli 1

Spirochetes 1

Sterilization & disinfection 1

Obligate intracellular bacteria 1

Anaerobic organisms 2

General introduction of parasites 1

Protozoans 2

Helminthes 3

Microscopy 2

Slide preparation for wet and stained films 2

Culture media 2

Identification of cultured isolates 2

Anaerobic culture 2

25

Identification of protozoa in clinical specimens 2

Identification of helminthes in clinical specimens 2

L: Lecture, T: Tutorial, C/P: Clinical or Practical and SDL: Self directed

learning


4.1 Written exam to assess knowledge and intellectual skills


4.3 during-semester evaluation to assess practical abilities.


4.1 written exam 60

4.2 oral exam 15


Total 100

5 --List of References



Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C. and Yolken, R.H. Manual

of clinical microbiology.


Bill & Scott Koneman



Journals: Journal of Clinical microbiology (available in the library of Ain Shams


Clinical chemistry2 course specifications: University: Ain Shams Faculty of Medicine






Academic year / Level Diploma –2nd

, 3rd

and 4th

semester



Title: Clinical Chemistry2 Code: CP9005 (a, b, c)

Credit Hours: 5.5 hour Lecture: 2/ week Total: 60

Practical: 3/every other week Total: 45

Co-ordinator

26



1- Course Aims:

a- Develop a firm, thoughtful and analytically creative understanding of the theory

and practice of clinical chemistry.

b- Develop laboratory doctors who are capable of successfully managing a

laboratory offering routine clinical chemistry tests.


a- Knowledge and understanding:


a1- Discuss different diseases and various organ dysfunctions; their aetiology,

pathophysiology, and associated laboratory findings (normal and abnormal).

a2- Identify the different laboratory tests used for screening, diagnosis and follow

up of various disorders including: renal, hepatic, cardiac, metabolic and hormonal

disturbances and some malignant disorders.

a3- Recognize the different techniques used in chemical pathology with regards to

principle, components, types, advantages and disadvantages. These techniques

include spectrophotometry, fluorometry, chemiluminescence, nephelometry,

turbidimetry, electrophoresis and electrochemistry.

a4- List the different basic medical statistical methods used in data analysis, both

qualitative and normally distributed quantitative results, together with

establishment of reference values and evaluation of the diagnostic performance of

each laboratory test.

a5- Recognize the basic concepts of automation of laboratory techniques.



b1- Select the appropriate tests used for screening, diagnosis, and follow up of

various disease states taking into consideration the concept of cost effectiveness.

b2 -Integrate clinical and laboratory findings for proper interpretation of different

laboratory results for correct medical decision- making.

b3 - Upgrade laboratory performance in healthcare units and small laboratories.

b4 - Plan medical research targeting high quality community service.

c- Professional skills:


c1- Master the techniques of specimen collection, handling and processing.

c2- Efficiently perform routine laboratory tests.

c3-Apply infection control and safety measures in the laboratory.

c4- Implement internal quality control measures.

d- General and transferable skills:

27

By the end of the course the candidate will be able to: d1- Think creatively and innovatively, with individual initiative and ability to work

in a team.

d2 -Undergo fruitful laboratory- physician interaction to ensure correct decision

making.

d3 -Undergo proper problem-based learning and IT application for maximum

knowledge collection and case presentation

d4 - Implement safe laboratory procedures

e- Attitude





service

3- Course content:

2nd

semestre: Course code: CP9005a

Topics No. of hours (Actual)

L T C/P SDL

Instrumentation 3 2

Enzymes 4

Liver 5

Biological fluids 3

Quality control 6

Spectrophotometry, end-point 2

Total protein, albumin 2

Glucose, glycated Hb 2

Urea, uric acid 2

3rd

semester: Course code: CP9005b

Topics No. of hours (Actual)

L T C/P SDL

Lipids 4

Proteins 4

Kidney 5

Parathyroid 2

Ca, Phosphorus 2

Cholesterol, triglycerides 2

28

Bilirubin, creatinine 2

Kinetic measurements 2

ALT, AST 2

ALP, ACP 2

Amylase, lipase 1

CK, LDH 2

4th semester : course code CP9005c

Topics No. of hours

L T C/P SDL

Acid-base 5

Diabetes 5

Hypoglycaemia 1

Tumour markers 2

Thyroid 2

Pregnancy, fertility 3

Medical statistics 6 2

Cardiac biomarkers 2

Adrenal 3

Pituitary, GH 1

Urine analysis 5

Semen analysis 3

Electrophoresis 5




4.2 practical exam to assess professional and practical skills


4.4 during-semester evaluation to assess mastering of practical skills and abilities.


4.1 written exam 145

4.2 Practical exam 120

4.3 oral exam 15


Total 340




29



2- Clinical Chemistry From Principles to Practice. 2nd Edition.2008



edition. Michael


5.4- Periodicals, Web Sites,




Laboratory HEMATOLOGY 2 COURSE SPECIFICATIONS University: Ain Shams Faculty of Medicine







, 3rd

and 4th

semesters



Title: Laboratory Hematology 2 Code: CP9006 (a, b, c)

Credit Hours: 5.5 hours Lecture: 2/ week Total: 60

Practical: 3/ week Total: 45

Co-ordinator:

Head of Hematology Unit (Prof. Basima El Essawy)


1- Course Aims

a- To develop a thoughtful and analytically understanding of the theory and

practice of hematology

b- To build interpretative skills so that a clinically useful opinion can be derived

from the laboratory data

c- To train the student in laboratory methodology relating to clinical hematology

including procedures, principles, QC and normal values

d- Develop laboratory doctors capable of successfully managing a laboratory

offering routine hematology tests

e- To encourage the students to develop high standards in their professional

practice

30

2- Intended Learning Outcomes (ILOs) from Course



a1- Develop an understanding of different hematological diseases; their etiology,

pathophysiology including molecular basis, and associated laboratory findings

a2- Identify the different laboratory tests used for screening, diagnosis and follow

up of various disorders including: red cell disorders, white cells disorders, hemato-

oncology, bleeding and thrombotic disorders

a3- Recognize and critically evaluate the different techniques used in hematology

with regards to principle, methodology, applications, interpretation and

limitations. These techniques include : CBC, bone marrow aspiration and trephine

biopsies, peripheral blood and bone marrow examination, iron profile tests,

hemoglobin electrophoresis and other tests to diagnose and classify various

anemias, coagulation tests, cytochemical staining, flow cytometry, cytogenetic

analysis, molecular techniques and pretransfusion testing.

a4- Know the basic concepts of automation of hematology techniques and QC

b- Intellectual skills:


b1- Demonstrate an investigatory and analytic thinking approach to clinical and

pathological situations selecting the appropriate tests used for screening, diagnosis,

and follow up of various hematological diseases taking into consideration the

concept of cost effectiveness.

b2- Release concise, relevant and understandable reports in response to a request

for investigation

b3- Integrate clinical and laboratory findings for proper interpretation of different

laboratory results on the basis of reference values, critical values and method

limitations for correct medical decision- making.

b4- Up-grade laboratory performance in healthcare units and small labs.



c1- Perform efficiently routine laboratory hematology and transfusion tests.

c2- Apply infection control and safety measures in the Lab.

c3- Implement internal quality control measures.



d1- Think creatively and innovatively, with individual initiative and ability to work

as part of a multi-disciplinary team.

31

d2- Undergo fruitful laboratory- physician interaction to ensure correct decision

making.

d3- Undergo proper problem-based learning and IT application for maximum

knowledge collection and case presentation

d4- Have the necessary skills to maintain continuing professional development

d5- Adhere to professional and ethical conduct

e- Attitude


e1- Work as part of a multi-disciplinary team within the clinical sphere of their

activity, as well as in the delivery of their laboratory duties

e2-Obtain the skills to maintain their continuing professional development.

e3- Adhere to professional and ethical conduct

e4- Maintain interpersonal communication skills

3- Course content

2nd

semester (course code 9006a)

Topics No. of hours

L T C/P SDL

Iron metabolism and iron deficiency anemia 2

Disorders of heme synthesis and iron overload 1

Megaloblastic anemia 3

Hereditary diseases of red cell membrane 1

Disorders of red cell metabolism 2

Hemoglobin and inherited disorders of globin

synthesis

2

Acquired hemolytic anemias 4

Anemia of chronic disorders 1

Aplastic anemia and bone marrow failure 2

Benign leucocyte disorders 2

Diff. WBC Abnormalities, RBC abnormalities 4

Automated CBC 2

Quality control in hematology 2

Normocytic normochromic anemia, Some

diagnostic tests in hemolytic anemia

4

Microcytic hypochromic anemia, Macrocytic

anemia

2

3rd

semester (course code 9006b)

Topics No. of hours

L T C/P SDL

32

The spleen 1

Lysosomal storage disorders 1

Molecular basis of leukemia and lymphoma 1

Acute leukemias 2

Minimal residual disease in acute leukemia 1

Myelodysplastic syndromes 1

Chronic myeloproliferative disorders 3

Chronic lymphoproliferative disorders 3

Plasma cell neoplasms 2

Platelet disorders 2

Inherited bleeding disorders 4

Bone marrow biopsy, Normal bone marrow, How

to report on BM

2

Normal bone marrow 2

Hypersplenism, ITP 2

Acute leukemia 3

Chronic lymphoproliferative disorders 3

Chronic myeloproliferative disorders 4

4th

semester (course code 9006c)

Topics No. of hours

L T C/P SDL

Acquired coagulation disorders 2

Thrombophilia 1

Stem cell transplantation 1

Blood transfusion in special clinical

conditions

2

Automated cell counters 3

Flow cytometry: principle and applications 3

Molecular techniques and applications 2

Cytogenetic techniques and applications 2

Trephine biopsy 2

Aplastic anemia, Megaloblastic anemia,

Iron stain of the BM

2

Bleeding time, PT, PTT, TT (II) 2

Factor assay, fibrinogen, FDPs, lupus

anticoagulant mixing experiments

3

Blood grouping and cross matching (II) 2

Separation and preservation of blood 2

33

components

Sources of error in hematology testing 2

Interpretation of some practical cases 2








4.1 written exam 145


4.3 oral exam 15


Total 340



5.2- Essential Books (Textbooks)

1- Essential Hematology, last edition

2- Dacie Practical Hematology, last edition


1- Postgraduate Hematology, last edition

5.4- Websites and periodicals

1- medline.com,

2- cpainshams.com

3- Blood (journal available at Ain Shams University Hospital Library)

Clinical Immunology2 Course specifications: Program on which the course is given: Diploma in Clinical Pathology





, 3rd

and 4th

semester



Title: Clinical Immunology2 Code: CP9007 (a & b)

Credit Hours: 3 hour Lecture: 2/every other week Total: 30


Coordinator

Head of Clinical Immunology Unit (Prof. Mona Rafik)

34


i- Course Aims:

To integrate knowledge of fundamental and clinical laboratory immunology, and

utilize it for diagnostic and therapeutic approaches and the student will have:

a- Specialized knowledge of the immune-mediated diseases

b- Interpretative skills so that a clinically useful opinion can be derived from the

laboratory data

c- Technical knowledge of laboratory immunology including the implementation

of quality control and quality assurance procedures

d- Generic issues common in all laboratories



a1– Understand the patho-physiology, including the molecular basis of immune

mediated diseases and have a specialized knowledge of the immune-mediated

diseases.

a2- Acquire knowledge for the diagnosis and follow up of patients with diseases or

disease processes resulting from disordered immunological mechanisms, and

conditions in which immunological manipulations form an important part of

therapy and/or prevention.

a3- Understand the principles, and carry out the appropriate laboratory tests

required for the prevention, diagnosis and follow-up of organ based immune-

mediated diseases (Kidney, liver, endocrine, GIT CNS skin and reproductive

organs) primary and acquired immunodeficiency diseases ,autoimmune diseases

(systemic rheumatic diseases and organ based autoimmune diseases) allergy, graft

rejection and malignancy

a4- Acquire the core body of knowledge to understand the principles of

immunotherapy, immuno-modulation and vaccination.

a5- Acquire knowledge and skills required to direct a diagnostic immunology

laboratory where the principal subject areas are Immunochemistry (ELISA RID)

Autoimmunity (IIF for auto-antibodies) Cellular immunology (lymphocyte

separation, phagocytosis) and Allergy

a6- Understand the principles and methodologies of different molecular biological

assays (PCR Immuno-blotting), HLA typing and cross matching, and flow

cytometry



b1- Obtain knowledge of the laboratory assays and is able to provide interpretative

advice on the clinical significance of the results obtained.

35

b2 - Select, interpret and provide clinical advice based on laboratory investigations

b3- Construct succinct, relevant and understandable reports in response to a request

for investigation.

b4- Familiarize with the role of the diagnostic laboratory in supporting hospital and

general practice services, including the interpretation of tests, provision of clinical

advice regarding test selection, and clinical liaison

b5-Understand, putative mechanisms of action of various immunological therapies

including immunoprophylaxis



c1- Give consultative advice on the appropriate use of laboratory tests for the

prevention, diagnosis and follow-up of organ based immune- mediated diseases

(Kidney, liver, endocrine, GIT CNS skin and reproductive organs) primary and

acquired immunodeficiency diseases ,autoimmune diseases (systemic rheumatic

diseases and organ based autoimmune diseases) allergy, graft rejection and

malignancy

c2-Perform procedures and investigations which are in routine use in the

immunology laboratory

c3- Direct a diagnostic immunology laboratory



d1-Be aware of relevant sources of information including computerized databases



e- Attitude


e1- work as part of a multi-disciplinary team within the clinical sphere of their

activity, as well as in the delivery of their laboratory duties

e2-obtain the skills to maintain their continuing professional development.

e3- Adhere to professional and ethical conduct

e4-. Maintain interpersonal communication skills

3- Course content:

Second semester: CP9007 a

Topics No. of hours

L T C/P SDL

Pathogeneis of immune mediated diseases

Hypersensitivity mechanisms

2

36

autoimmunity

Immunology of infections

Infections of the immune system

Laboratory Clincal Immunology

Immunodeficiency disorders

Scientific basis of therapy of primary

immunodeficiency and New developments in therapy of

immunodeficiency

Diagnosis and management of allergic diseases in

adults and children

Scientific basis of allergen immunotherapy and New

developments in therapy of allergic disease

Tumour immunology

Laboratory Diagnosis of Lymphproliferative disease

and myelomatosis

Scientific basis of immunoprophylaxis

Molecular Biological techniques

1

1

1

2

1

2

1

1

1

1

1

Detection and quantification of Paraproteins Enzyme immunoassay &Radioimmunoassay (RIA)

Isolation of mononuciear cells

Knowledge and interpretation of HLA typing

2

6

6

1

Third semester: CP9007 b

Topics No. of hours

L T C/P SDL

Autoimune Diseases:

Immunopathogenesis of diseases affecting body

systems

Liver

Diagnosis of autoimmune liver diseases

Diagnosis of Viral hepatitis

2

37

Endocrine

Diagnosis of autoimmune endocrine

disease

GIT

Diagnosis of immune mediated

gastrointestinal diseases

Kidney

Diagnosis of immune mediated kidney

diseases

CNS

Diagnosis of immune mediaed

neuromuscular diseases

Skin

Diagnosis of autoimmune skin diseases

Reproductive organs

Diagnosis of immune mediated

infertility

Scientific basis of immunosuppressive and

immunomodulatory therapy

Drugs

Antibodies

Laboratory Immunology:

Antigen antibody reactions

Interpretation of lab results

Case reports

Prolem solving

1

1

1

1

1

1

1

1

1

1

1

1

1

Indirect immunofluorescence for autoantibodies

Extractable nuclear antigens

Anti RBCs

o Direct Coomb’s

o Indirect Coomb’s

Knowledge of RNA and DNA extraction

Knowledge and interpretation of PCR techniques

Knowledge of Assessment of lymphocyte function

Knowledge of Flow cytometry

8

1

1

1

1

1

1

1


4.1 written exams to assess knowledge and intellectual skills

4.2 practical exams to assess professional and practical skills

4.3 oral exams to assess knowledge, attitude and communication skills

38

4.4 During-semester evaluation to assess mastering of practical skills and abilities.


4.1 written exam 70


4.3 oral exam 15

4.4 During-semester 40

Total 185


5.1- Course Notes: LECTURES


Immunobiology: The Immune System in Health and Disease by Charles Janeway (Editor), Paul Travers, J. Donald Capra, Mark J. Walport


Concise Clinical Immunology for Healthcare Professionals Mary T. Keogan,

Eleanor M. Wallace and Paula O’Leary Routledge2006


http://12.17.12.70/aai/educating/ - (Lab Exercises) Exercises to make

immunology clearer

http://www.biology.arizona.edu/immunology/immunology.html - (Lab Exercises

Immunology problem sets and tutorials

http://www.allergy-drug.com/ - (Teacher Information, Student Help) Good

explanation of allergies and allergic reactions

http://www.cat.cc.md.us/courses/bio141/lecguide/unit3/index.html - (Teacher

Information Student Help) Immunity lecture notes; covers the basics, humoral and

cell-mediated immunity, immunodeficiencies, hypersensitivity, etc.

http://www.fbr.org/swksweb/swklab.html - (Lab Exercises) Immunology cases

and simulations

http://www.niaid.nih.gov/publications/autoimmune/autoimmune.htm - (Student

Help) Easy-to-understand explanation of autoimmunity

Clinical microbiology 2 course specification University: Ain Shams Faculty of Medicine







, 3rd

and 4th

semester

Date of specification approval ……………………………………….

http://12.17.12.70/aai/educating/

http://www.biology.arizona.edu/immunology/immunology.html

http://www.allergy-drug.com/

http://www.cat.cc.md.us/courses/bio141/lecguide/unit3/index.html

http://www.fbr.org/swksweb/swklab.html

http://www.niaid.nih.gov/publications/autoimmune/autoimmune.htm

39


Title: Clinical microbiology 2 Code: CP9008 (a & b)

Credit Hours: 3 hours Lecture: 2/every other week Total: 30


Co-ordinator

Head of Clinical microbiology Unit


i- Course Aims:


a- Interpret and analyze lab findings and recognize its limitations (as overlap in

reference range).

b- Recognize appropriate diagnostic strategies for common diseases and

recommend further testing for diagnosis and follow up.

c- Understand the necessary sample specifications and precautions including

patient preparation, type of sample, sample collection, sample transport, handling,

and turn-around time.

d- Integrate clinical findings with lab results into meaningful diagnostic

formulation (cases).




a1- Identify criteria for sample rejection and acceptance for microbiological

specimens.

a2- List different etiological causes of infection in different body systems.

a3-Recognize pathogenesis and different methods of laboratory diagnosis of

bacterial, viral, mycotic, parasitological infections.

a4- Define nosocomial infection and identify mode of transmission.

a5-Recognize different groups of antibiotics, mode of action, spectrum, and choice

in different clinical situations and mechanisms of resistance.

Identify safety measures applied in microbiological laboratory.

a6-Identify the role of clinical microbiologist in implementation of different

policies and procedures of infection control in hospitals.

a7- Recognize steps for quality management in clinical microbiological laboratory.

a8- Review advanced automated methods in microbiology.



b1- Interpret different serological techniques.

b2- Analyze different antibiogram results.

b3- Differentiate different G + ve & G –ve microorganisms.

40



c1- Inoculate different culture media.

c2- Deal with different microbiological specimens.

c3- Prepare perfectly antibiogram techniques.



d1- Write a lab request with the necessary relevant information.

d2- Use and deal with new technology tools.

d3- Become familiar with the work environment in the laboratory.

d4- Undergo fruitful interaction with technicians and fellow doctors.

d5- Become acquainted with IT applications for maximum knowledge collection.

d6- Implement safe laboratory procedures.

e- Attitude





service

C- Course content:

2nd

semester: Course code: CP9008a

Topics No. of hours

L T C/P SDL

Pyrexia of unknown origin 1

Neonatal infections 1

Anaerobic infections 1

C.N.S. infections 1

Upper & lower respiratory infections 1

Mycobacterial infection T.B/ lebrosy/ atypical 1

G.I.T. infections 1

Cardiac infections 1

Bone & joint infection 1

Eye infection 1

Urinary tract infection 1

Genital tract infection 1

Sexually transmitted disease 1

Antimicrobial chemotherapy 2

Staining Techniques 2

Identification of organisms on culture media 2

Biochemical Reactions 2

41

Antibiotic Sensitivity 2

Widal & Brucella agglutination tests: 1.5

Haemagglutination,Haemagglutination – inhibition,

ASOT & ELISA

1.5

Urine culture 2

Genital specimen culture 2

3rd

semester: Course code: CP9008b

Topics No. of hours

L T C/P SDL

Laboratory safety in microbiology lab. 1

Quality in microbiology lab. 1

Hospital infection and control 1

General virology 1

Orthomyxo & paramyxoviruses 1

Exanthemas 1

Herpes virus infection 2

Entero viruses infection 2

Basic mycology 1

Isolation and identification of pathogenic fungi 1

Superficial & cutaneous mycotic infections 1

Subcutaneous mycotic infections 1

Systemic mycotic infections 1

Stool culture 2

Pus and wound culture 2

Blood culture 2

Body fluid culture 2

Upper respiratory tract specimen cultures 2

Lower respiratory tract specimen cultures 2

Anaerobic packs and jars 1

Mycology specimens examination and culture 2








4.1 written exam 70


42

4.3 oral exam 15


Total 185




Murra, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C. and Yolken, R.H. Manual of

clinical microbiology.




Journals: Journal of Clinical microbiology, (available in the library of Ain Shams


Elective Courses: 1-Total Quality Management in clinical laboratory course specifications: University: Ain Shams Faculty of Medicine



Major/Minor elements of program: Minor



Academic year / Level: Diploma-elective course, 4th

semester.


A-Basic Information

Title: Total quality management in clinical laboratory Code: E9118

Credit Hours: 1 hour Lecture: 1 hr/ week Total: 15

Coordinator

Head of Clinical Chemistry Unit


1- Course Aims:

a- Develop a good laboratory manager, who is motivated and dedicated to the

implementation of the concept of quality and the enhancement of the services

given by the laboratory.



43


a1 - Identify different processes in the laboratory system.

a2-Recognize the cycle of quality improvement.

a3- Recognize the importance of laboratory design and lay-out.

a4- Appreciate the financial aspects involved in the laboratory system.

a5-Recognize the basic concepts of safety in the laboratory.



b1- Upgrade laboratory performance through quality improvement system.

b2 -Appropriately select and evaluate commercial kits and instruments present in

the market

b3 - Integrate financial and technical knowledge to reach high managerial levels

with the aim of efficiently managing the laboratory with maximum profit.



c1- Properly select and evaluate kits and instruments.

c2- Design laboratory lay-out.

c3-Apply infection control and safety measures in the laboratory.

c4- Implement internal and external quality control measures.


By the end of the course the candidate will be able to: d1- Think creatively and innovatively, with individual initiative and ability to lead

a team.

d2 -Undergo fruitful laboratory- physician interaction to ensure correct decision

making.

d3 -Undergo proper problem-based learning and IT application.

d4 - Implement safe laboratory procedures

e- Attitude





service

2- Course content:

Topics No. of hours

L T C/P SDL

Introduction 1

Laboratory lay-out, design 1

Control of pre-analytical phase 1

Control of analytical phase 1

Control of post analytical phase 1

44

Internal and external quality control 2

Reference values 2

Evaluation, selection of methods 2

Laboratory safety 2

Cost effectiveness, inventories 2


learning



Assessment Schedule and Weighing of Assessments:

1-Written exam 50

Total 50






2- Clinical Chemistry.From Principles to Practice. 2nd Edition.2008



edition. Michael






2-Molecular Diagnostics Course specifications: University: Ain Shams Faculty of Medicine







semester.

Date of specification approval………………………………


Title: Molecular diagnostics Code: E9002

Credit Hours: 1 hour Lecture: 2/every other week Total: 15

Coordinator

45

Heads of Clinical Chemistry and Immunology Units


i- Course Aims:

To cover the basic concepts of molecular pathology & clinical diagnostics

including nucleic acids, chromosomes, DNA replication, transcription, proteins,

mutations & chromosome changes that underlie inherited & acquired/infectious

disease, inheritance patterns & genetics as applied to oncology, cardiac disease &

organ transplants. Covers emerging molecular/cytologic methods (amplification,

hybridization & microarrays) to detect disease markers, monitor therapy & assess

identity; pharmacogenomics & legal/ethical issues of genetic testing




a1- Define terms related to molecular diagnostics.

a2-Identify appropriate specimen collection and handling measures for molecular

diagnostics.

a3-Classify the various molecular methodologies.

a4-Outline examples of procedures for each molecular diagnostic classification.

a5-Describe the principle of each procedure.

a6-Discuss clinical applications of molecular diagnostics

a7- Know the principles behind molecular diagnostics, giving a strong foundation

for future exploration and study in molecular diagnostics



b1- Apply molecular biology techniques for diagnosing inherited (genetic) and

acquired disorders.

b2-Understand assays performed such as DNA purification methods, Southern blot

analysis, polymerase chain reaction and other specialized techniques and their

limitations, which is essential to the interpretive process

b3- Understand the basic concepts of molecular pathology

b4- Interpret clinical diagnostics including nucleic acids, chromosomes, DNA

replication, transcription, proteins, mutations & chromosome changes that underlie

inherited & acquired/infectious disease, inheritance patterns & genetics

b5- Advise clinical colleagues on relevant tests for a given clinical situation.

b6- Differentiate between performance of tests to screen for disease versus

diagnosis


46


c1-Apply molecular biology techniques for diagnosing inherited (genetic) and

acquired disorders

c2-. Determine pre-analytical variables that affect molecular methodologies.

c3-Explain the principles and background governing the performance of each test

c4-Identify the criteria for rejection of unacceptable specimens.

c5-State the method of performing each of the listed procedures.

c6-Identify quality assurance procedures that are required.

c7-Identify possible sources of error and conditions under which each of the tests

might yield false results.

c8- Interpret results of each of the tests

c9- Give appropriate laboratory data, evaluate the results of a patient's testing.

(clinical correlations)



d1- Be aware of relevant sources of information including computerized databases



e- Attitude




e2-Appreciate integral importance of teamwork in running a diagnostic laboratory

service

3- Course content:

Topics No. of hours

L T C/P SDL

1- Human genome and chromosomes

2- Nucleic acids

3- DNA replication

4- transcription, translation

5- mutations & chromosome,

6- genetics & inheritance patterns changes that

underlie inherited & acquired/infectious disease

7- clinical applications of molecular diagnostic

(disease markers)

8- legal/ethical issues of genetic testing

9- Specimen Collection & Handling

1

1

1

1

1

1

1

1

1

47

10- molecular methodologies

11- Procedure Principles

12- Clinical Applications

2

3

1


4 - 4 - Student Assessment Methods



1-Written exam 50

Total 50


5.1- Course Notes

LECTURES


Manual of immunological methods by Pauline Brousseau, M. Beaudet

-Teitz Textbook of Clinical Chemistry and Molecular Diagnostics,4th edition,





Immunobiology: The Immune System in Health and Disease by Charles Janeway (Editor), Paul Travers, J. donald Capra, Mark J. Walport



and simulations

http://www.biology.arizona.edu/immunology/immunology.html - Lab Exercises

Immunology problem sets and tutorials.

3- Applied Laboratory Immunology Course specifications: University: Ain Shams Faculty of Medicine







semester.

Date of specification approval…………………………………


Title: Applied Laboratory Immunology Code: E9015




48

Coordinator

Head of Clinical Immunology Unit


i- Course Aims:

To acquire the core body of knowledge necessary to understand the principles and

limitations of immunological tests and techniques and to give consultative advice

on the appropriate use of laboratory tests for prevention, diagnosis and treatment of

the immune-mediated diseases:




a1-Select and conduct appropriate analysis to contribute to the diagnosis of and

protection against ailment, the treatment, verification of and linking analysis results

to the case in hand as well as to the improvement of the examinations and analysis

performed by the laboratory

a2 -Know of the principles of laboratory testing in diagnostic immunology with the

diagnostic utility and limitations of laboratory tests e.g. sensitivity, specificity,

predictive values in different immunological diseases

a3 - Explain the concept of Quality Assurance and Quality Control and apply these

in practice

a4- Understand the statistical methods used for interpreting laboratory data.

a5- Know of different immunological techniques with their limitations and how to

trouble shoot



b1- Clarify articulate an understanding of issues relating to standardization and

quality control/assurance for each of the diagnostic technologies, for example

immunofluorescence, enzyme linked immunoassay, radioimmunoassay,

agglutination and so on.

b2-Understand assays performed and their limitations, which is essential to the

interpretive process

b3- Construct patient reports are generated and when additional comments or

telephone communication may be required.

b4- Advise clinical colleagues on relevant tests for a given clinical situation.

b5- Differentiate between performance of tests to screen for disease versus

diagnosis

b6- Communicate results and concepts clearly, both verbally and in writing.

49



c1- Develop an understanding of how changes in the immune system responds can

be used in the laboratory for monitoring and diagnosis.

c2- Broaden the experience and understanding of applied clinical and diagnostic

laboratory immunology.

c3- Explain the principles and background governing the performance of each test

c4- Identify the criteria for rejection of unacceptable specimens.

c5- State the method of performing each of the listed procedures.

c6- Identify quality assurance procedures that are required.

c7- Identify possible sources of error and conditions under which each of the tests

might yield false results.

c8- Identify reference or normal ranges.

c9-Interpret results of each of the tests.

c10-Give appropriate laboratory data, evaluate the results of a patient's testing.

(clinical correlations)



d1- Be aware of relevant sources of information including computerized databases



e- Attitude





service

3- Course content:

Topics No. of hours

L T C/P SDL

1-STATISTICAL METHODS USED FOR

INTERPRETING LABORATORY DATA

2- QUALITY ASSURANCE AND QUALITY

CONTROL in the IMMUNOLOGY LAB

3- SELECTION OF LABORATORY TECHNIQUES

4- SELECTION AND INTERPRETATION OF

LABORATORY TESTS: Clinical Relevance.

2

2

6

2

1

1

1

50





1-Written exam 50

Total 50


5.1- Course Notes

LECTURES


Manual of immunological methods by Pauline Brousseau, M. Beaudet




Immunobiology: The Immune System in Health and Disease by Charles Janeway (Editor), Paul Travers, J. Donald Capra, Mark J. Walport



and simulations

http://www.biology.arizona.edu/immunology/immunology.html - Lab Exercises

Immunology problem sets and tutorials

4- Medical Research course specifications: University: Ain Shams Faculty of Medicine







semester


A-Basic Information

Title: Medical research Code: E9121

Credit Hours: 1 hour Lecture: 1/ week Total: 15

Co-ordinator





51

1- Course Aims:

Develop an efficient researcher who can properly design and implement a medical

research, taking in account the required ethical considerations, and who is capable

of proficient selection of the study sample, statistical analysis of data and skillful

presentation of the results.




a1- Identify the types of research methods and study designs.

a2- Recognize the criteria of sample selection and sample size calculation.

a3- Understand the different statistical tests used for data analysis and their

application.

a4- Know the ethical requirements of medical research.



b1- Acquire the skills of designing different research methods either observational

or interventional to deliver generalizable knowledge.

b2 –Formulate the research question and develop the research protocol.

b3 – Critically read and appraise the literature.

b4 – Specify the inclusion and exclusion criteria for selection of study subjects and

estimate the proper sample size.

b5- Select the appropriate statistical methods for data analysis.

b6- Present the results in the appropriate way.



c1- Design a medical research.

c2- Write a research protocol.

c3- Prepare an informed consent form.

c4- Apply the appropriate statistical tests for data analysis.

c5- Construct a scientific paper.


By the end of the course the candidate will be able to: d1- Think creatively and innovatively, with individual initiative and ability to lead

a team.

d2 – Respect the ethical requirements of the research and ensure confidentiality of

the subjects’ information.


e- Attitude




52


service

3- Course content:

Topics No. of hours

L T C/P SDL

Research methodology & study designs 4

Critical review of literature 2

Selection of study subjects & sample size 2

Data management & statistical methods 3

Research ethics 2

Presentation skills 2





1-Written exam 50

Total 50






2- Clinical Chemistry From Principles to Pracice. 2nd Edition.2008



edition. Michael






5- Therapeutic Drug Monitoring (TDM) and Clinical Toxicology course specifications: University: Ain Shams Faculty of Medicine







semester

53


A-Basic Information

Title: Therapeutic drug monitoring and clinical toxicology Code: E9122

Credit Hours: 1 hour Lecture: 1/ week Total: 15

Coordinator

Head of Clinical Chemistry Unit


1- Course Aims:

Develop a laboratory doctor who understands the principles and procedures of

therapeutic drug monitoring and clinical toxicology, with the aim of:

a- giving consultations to colleagues concerning therapeutic drug monitoring and

clinical toxicology.

b- introducing these special techniques in the clinical chemistry laboratory.




a1 –Recognize the basic concepts of therapeutic drug monitoring and clinical

toxicology.

a2-Identify the methods used for analysis of different drugs, toxins, and metals.

a3- Discuss the factors that affect the drug pharmacokinetics.

a4- Understand the concept of pharmacogenetics.



b1- Select the appropriate sample specifications according to the drug or toxin

type.

b2- Select the appropriate tests for drug monitoring and detection of toxicity.

b3 – Relate the concentration of the circulating drug to its pharmacokinetic

parameters.

b4 - Interpret the results of the different tests used and provide recommendations

for further testing.



c1- Efficiently apply the tests used for monitoring of drugs and detection of

toxicity.

c2- Implement quality control measures for drug assays.


54

By the end of the course the candidate will be able to: d1 -Undergo fruitful laboratory- physician interaction to ensure correct decision

making.


3- Course content :

Topics No. of hours

L T C/P SDL

TDM: Basic concepts 2

Specific drug groups 2

Pharmaco-genetics 4

Clinical Toxicology: Basic concepts 2

Screening and on-site tests 2

Toxicology of specific drugs and toxic agents 2

Toxic metals 1





1-Written exam 50

Total 50






2- Clinical Chemistry.From Principles to Pracice. 2nd Edition.2008



edition. Michael






6-Infection Control Course specifications: University: Ain Shams Faculty of Medicine



Major or minor element of programs: Minor


55


Academic Level: Diploma –elective course– 4th semester

Date of specification approval: …………………………..


Title: Infection Control Code: CP E9059


Coordinator

Prof. Hadia Bassim


i- Course Aims:


a- Define nosocomial infections and diagnose nosocomial blood stream

infections(BSI).

b- Diagnose surgical site infections (SSI) and list risk factors for SSI.

c- Diagnose nosocomial pneumonia (NP) and list risk factors for nosocomial

pneumonia.

d- Identify types of isolation measures.

e- Identify the potential risk and impact of nosocomial infections on expected

patient outcome as well as hospital stay.

f- Identify the difference between sterilization and disinfection.

g- Identify chemical agents used for disinfection, and the effectiveness of each

chemical and its uses.




a1-Define nosocomial infections, ventilator associated pneumonia, and catheter

related infections.

a2 - Recognize appropriate personal protective equipments.

a3 -Identify different groups of antibiotics, mode of action, spectrum, choice in

different clinical situations and mechanisms of resistance.

a4 -Define different types of isolation precautions in health care facilities

a5-Identify the important pandemics.



b1- Investigate and an outbreak.

56

b2- Select suitable strategy for antibiotic susceptibility for different

microorganisms.

b3- Interpret lab safety.

b4- Formulate an isolation plan for common diseases.

b5-Understand the proper types for waste management, cleaning, disinfection, and

sterilization



c1- Apply different plans for isolation.

c2- Perform the proper ways for waste management.

c3- Properly manage an outbreak.



d1 –Gather material from various sources including electronic database and online

resources.

d2 – Diagnose nosocomial infections and ventilator associated pneumonia.

d3- Use and deal with new technology tools.

d4- Recognize the proper ways for hospital building and construction.

3- Course content:

4th

semester: Course code: CP E6059

Topics No. of hours

L T C/P SDL

Introduction on the importance of infection control in

health care facilities

1

Epidemiology of nosocomial infections 1

Hand Hygiene 1

Personal Protective Equipment (PPE) 0.5

Aseptic Techniques 1.5

Isolation Precautions in Health Care Facilities (HCF) 1.5

Waste Management 1

Cleaning, Disinfection, &Sterilization of medical

instruments

1

Clinical microbiology & Infection control 1.5

Antibiotic control policy 1.5

Dealing with outbreaks 1

Organization for efficient surveillance 1

Preventing infection of health care workers 1.5

57


learning




1-Written exam 50

Total 50

5 -List of References

5.1- Course Notes

LECTURES


Hospital Infection Control by MARY CASTLE,R.N.,M.P.H. & ELIZABETH

AJEMIAN,B.S.


5.4- Periodicals, Web Sites: www.CDC.com , www.AJIC.com www.WHO.

com , www.MOH.com

Medical journals: American journal of infectious diseases, International journal of

infectious diseases

7-Blood Transfusion Course Specifications University: Ain Shams Faculty of Medicine







semester.


A-Basic Information

Title: Blood transfusion Code: E9124

Credit hours: 1 hour Lecture: 1 hr/ week Total: 15

Coordinator:

Head of Laboratory Hematology Unit

Prof. Basima El Essawy


1- Course Aims

To provide the student with sufficient knowledge and experience to function as a

blood bank specialist in a community or academic hospital.

58

2- Intended Learning Outcomes (ILOs) from Course



a1- Demonstrate knowledge of blood donation, current eligibility criteria for blood

donors

a2- Discuss the principles of patient/unit identification and pre-transfusion testing,

including ABO/Rh testing, RBC antibody screen, and antibody identification

a3- Outline the assay principles (e.g. NAT, ELISA) of required donor blood tests

and the associated confirmatory testing

a4- Recognize the indications for component therapy and principles of preparation

of blood components

a5- Appreciate transfusion requirements of special patient populations

a6- Identify the major complications of blood transfusions, the current risk of these

complications, and explain how these infections can be prevented

a7- Recognize the symptoms and signs of hemolytic and non-hemolytic transfusion

reactions, the pathophysiology, treatment, and prevention of these complications

a8- Summarize the principles of apharesis technology and indications for

therapeutic pharesis

a9- Recognize the requirements of applicable regulatory and accrediting agencies



b1-Choose appropriate cross-matching methods for various patients

b2- Choose appropriate blood components and derivatives

b3- Manage blood inventory and apply triage and screen requests for blood

components appropriately during inventory shortages

b5- Apply the concept of appropriate use of blood and blood utilization reviews



c1- Implement a blood donation program

c2- Manage a red cell serology and compatibility lab

c3- Evaluate and recommend blood transfusion plan in special clinical situations

c4- Implement quality assurance plan in blood transfusion



d1- Think creatively and innovatively, with individual initiative and ability to lead

a team.

d2- Undergo fruitful laboratory- physician interaction to ensure correct decision

making.

d3- Undergo proper problem-based learning and IT application.

59

d4-Communicate efficiently results to clinicians both verbally and written in a

timely manner

3- Course content

Topics No. of hours

L T C/P SDL

Blood donation 1

Blood Components 2

Complications of blood transfusion 2

Pre-transfusion testing 2

Apheresis 2

Transfusion medicine in specific clinical settings 3

Blood transfusion in bone marrow

transplantation

1

Documentation, regulatory principles and quality

assurance

2





1-Written exam 50

Total 50




1-Poatgraduate hematology, last edition

5.3- Clinical Recommended Books

1. Hillyer CD, Silberstein LE, Ness PM, Anderson KC, Roback JD: Blood

Banking and Transfusion Medicine: Basic Principles and Practice (2nd

Ed.),

Philadelphia: Churchill Livingstone, 2007.

2. Mintz PD: Transfusion Therapy: Clinical Principles and Practice (2nd

Ed.), Bethesda, MD: AABB Press, 2005.



Journals: Transfusion, Vox Sanguinis

V- GENERAL INFORMATION

1 - MONITORING OF TRAINING AND SUBMISSION OF TRAINING

REPORTS

You must keep proper and updated records in your logbook to reflect the activities

encountered in your training. Your logbook must be duly endorsed by an

authorized signatory at the end of each semester.

60

You will be continuously assessed by your supervisors, in consultation with head

of department. An assessment will be submitted within 2 weeks of completion of

each semester.

2 - MISCELLANEOUS INFORMATION:

INJURY AND/OR BLOOD OR BODY FLUID EXPOSURE:

During regular working hours, you should immediately report an exposure

incident to infection control unit. If exposure occurs after regular working hours or

during a weekend or holiday; please call the emergency department .For injury,

please report to the Emergency Department.

Please also be sure to inform the supervisors of an exposure incident and/or

injury.

3 - ACTION ON COMPLETION OF CLINICAL TRAINING

Once all training sessions are completed the log book should be signed by the

senior supervisor and the head of the department and then should be submitted to

post graduate Secretariat.

4- REFERENCE

The Training Guide is available at the post graduate Secretariat and could be

downloaded from the following website is

---------------------------------------------------------------

VI – YOUR LOG BOOK

1- Introduction

The aim of this book is to give you a guide to the expectations for each item. It will

be a guide for both you and your teachers to what you should be seeing and doing.

It will give you a list of the important topics that you should think about and should

be covered in:

1. Practical sessions

or

2. Assignments

or

3. Self-directed learning (SDL)

For each item there is also a list of

1. Practical sessions to be seen or attended

2. Assignments to be completed

Remember

This document is only a guide. It is not an exhaustive list. It is not just a checklist

to score points. It is a guide to encourage you to read and learn more. This book is

for your benefit. It will form a record of your clinical training and experience.

61

2 - Supervisors

Head and staff members of clinical chemistry unit.

Head and staff members of laboratory hematology unit.

Head and staff members of clinical immunology unit.

Head and staff members of clinical microbiology unit.

3–Tables for Training Records

Candidates are required to fulfill 75% of the listed activities in order to be eligible

for the exam entry. The minimum number required for each activity = 75%. You

are free to attend more and record your extra attendance.

WEEKLY department PLAN of Clinical Chemistry

Saturday Sunday Monday Tuesday Wednesday Thursday

Scientific

Meeting

9am-11am

Lecture

basic 1

clinical

chem. 2

elective

9-10 am

9-11am

10-11am

Practical

basic 1

clinical

chem. 2

10-11am

11am-14pm

WEEKLY department PLAN of Laboratory Hematology


Scientific meeting 9am-11am

Lecture

Basic Hemat. 1

Lab Hemat. 2

Elective

9-10 am

9-11 am

10-11 am

Practical

Basic Hemat. 1

Lab Hemat. 2

10-11 am

1am-2 pm

WEEKLY department PLAN of Clinical Immunology

Saturday Sunday Monday Tuesday Wednesday Thurs.

Scientific Meeting 9am-11am

Lecture

Basic Imm.1

Clinc. Imm.2

9-11am

62

Elective 11-1 pm

Practical

Basic Imm.1

Clinc. Imm.2

11-1 pm

WEEKLY department PLAN of Clinical Microbiology


Scientific Meeting 9am-11am

Lecture

Basic Clin. Micro.1

Clin.

Micro.2

Elective

9-11

11-1

Practical

Basic Clin. Micro.1

Clin.

Micro.2

11-1

Requirements

Candidates are required to fulfill 75% of the listed activities in order to be eligible

for the exam entry. The minimum number required for each activity = 75%. You

are free to attend more and record your extra attendance.

Conference attendance

(NB. Minimum number required is 4)

No

Da

te

Pla

ce

Topics Supervisor

signature

1

2

3

4

Thesis attendance


No Date Place Name of the thesis Supervisor

signature

1

2

3

4

5

6

7

63

8

9

10

Case presentations


No Date Place Diagnosis Supervisor

signature

1

2

3

4

5

6

7

8

9

10

11

12

Scientific meetings


No Date Place Topic Supervisor

signature

1

2

3

4

5

6

7

8

9

10

11

12

Practical skills of Clinical Chemistry2

Weighing and preparing standards

64


No Date Place Performance Supervisor

signature

1

2

Acid preparation



signature

1

2

Specimen Collection and processing



signature

1

2

3

4

5

6

7

8

Spectrophotometers



signature

1

2

3

4

Endpoint assays


65

No Date Place Analyte Supervisor

signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

Kinetic assays

66


No Date Place Analyte Supervisor

signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

67

Urine Analysis



signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

68

Semen Analysis



signature

1

2

3

4

5

6

7

8

AP=ASSISTED PERFORMANCE

IP=INDEPENDENT PERFORMANCE

O=OBSERVATION

P=POOR S= Satisfactory E= Excellent GOOD

Assignments of Clinical Chemistry


No Date Score Topic Supervisor

signature

1 Centrifuge (1)

2 Acid preparation (1)

3 Pre-analytical problems (1)

4 Spectrophotometry (1)

5 Calculations (5)

6 SOP (end point) (1)

7 Urine cases (5)

8 Chemistry cases (5)

9 Quality control charts (5)

10 Statistics (5)

11 SOP (kinetic) (1)

12 Semen reports(5)

13 Chemistry cases (5)

14 Quality control charts (5)

69

15 Electrophoresis comment (5)

PRACTICAL SKILLS OF LABORATORY HEMATOLOGY2

Hemoglobin Curve


No Date Place performance Supervisor

signature

1

2

3

4

5

Hemoglobin Measurement

(Cyanmethemoglobin Method)



signature

1

2

3

4

5

6

7

8

9

10

Manual WBC Count



signature

1

2

3

4

5

6

7

8

70

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Manual Platelet Count

( Minimum number required is 30)


signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

71

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Blood Film Preparation & Leishman Staining



signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

72

20

21

22

23

24

25

26

27

28

29

30

Manual Differential Count

((Normal)



signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

73

24

25

26

27

28

29

30


(Abnormal WBCs/Benign)



signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

74

29

30


(Abnormal WBCs/RBCs Malignant Disorders)



signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

75


(RBCs Morphology)



signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Automated CBC

(Interpretation& Filtration)



76

signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Reticulocyte Preparation & Count (NB. Minimum number required is 20)


signature

1

2

3

4

77

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

PCV by Microhematocrit



signature

1

2

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ESR By Westergren Method



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Coombs Test (Direct)



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Coombs Test (Indirect)



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PT Determination



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PTT Determination



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Blood Grouping (Slide/Tube Method)



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Cross Matching (Manual)



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Bone Marrow Aspirate Interpretation



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Bone Marrow Iron Staining



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Peroxidase Staining



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BM Aspiration (Observation)



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Flow Cytometric Immunophenotyping

(Observation)



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Cytogenetic Analysis (Observation)


No Date Place performanve Supervisor

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Coagulation Factor Assay (Observation)



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Platelet Aggregation (Observation)



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PFA 100 (observation)



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G6PD Assay (Observation)



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Hemoglobin Electrophoresis (Observation)



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Blood Grouping by Column Agglutination



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Cross Matching by Column Agglutination



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Blood Component Separation & Preservation (Observation)

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O=OBSERVATION

P=POOR S= Satisfactory E= Excellent G=GOOD

ASSIGNMENTS OF LABORATORY HEMATOLOGY



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1 Blood collection techniques

2 Hemocytometer

3 Hb curves interpretation

4 ESR, Hct, RBC indices, film

preparation & staining

5 PT & PTT

6 Blood grouping and cross matching

7 Automation in hematology

8 Quality control

9 Abnormal differential count

10 Pretransfusion testing, problems and

pitfalls

11 Factor assay, Lupus anticoagulant

12 Platelet function testing

13 Acute leukemia

14 Myelo & lymphoproliferative

disorders

15 Anemias

16 Sources of error in hematology tests

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Practical skills of Clinical Immunology2 ELISA (Qualitative test)



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ELISA (Quantitative test)



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AUTOANTIBODIES (ANA ,ASM,AMA,APA.& LKM)



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AUTOANTIBODIES (ANTI-DNA)



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AUTOANTIBODIES (ANTITHYROID)



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LYMPHOCYTE SEPARATION


Phagocytosis ASSAY



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Nephelometry & RID



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IMMUNOELECTROPHORESIS AND IMMUNOFIXATION


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PCR


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HLA (MICROCYTOTOXICITY)



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HLA (PCR)



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O=OBSERVATION

P=POOR S= Satisfactory E= Excellent

Assignments of Clinical Immunology



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1 Neutrophil function

2 Protein electrophoresis

3 Complement assays

4 Fluid phase Immunoprecipitation

5 Gel phase Immunoprecipitation

6 Elisa

7 Autoantibody analysis

8 Lymphocyte separation

9 PCR

10 HLA

12 Troubleshooting

13 Immunofixation

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14 Lab requests

15 Lab reports

Practical skills of Clinical microbiology 2

Handling microscopy

(NB. Minimum number required is …2……….)


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Wet film preparation& examination

(NB. Minimum number required …….4 ….)


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Preparation & fixation of stained films

(NB. Minimum number required …2…)


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Preparation of Gram stained films &differentiation

between G+ve & G-ve organisms



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Preparation of ZN stained films & identification of AFB



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Identification of different culture media & its important

Constituents



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Inoculation of different culture media



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Identification of isolates on different culture media



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Identification of anaerobic culture & transport media, gas generating packs &

gas pack jar



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Identification& streaking of different biochemical reactionmedia

(NB. Minimum number required…4…)


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Interpretation of different organisms by different biochemical reactions



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Preparation & interpretation of antibiotic sensitivity


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Interpretation of Widal & Brucella agglutination tests



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Interpretation of haemagglutination, haemagglutination inhibition,

antistreptolysin O titre ELISA tests



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Dealing with urine specimens



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Dealing with genital specimens



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Dealing with stool specimens



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Dealing with pus &wound specimens



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Dealing with blood culture specimens



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Dealing with CSF& body fluids specimens



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Dealing with upper respiratory tract specimens



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Dealing with lower respiratory tract specimens



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Preparation of KOH wet films for mycological specimens



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Making scotch tap preparation



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Preparation of germ tube test



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Preparation of slide culture technique



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Identification of anaerobic culture & transport media, gas generating packs &

gas pack jar



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AP= ASSISTED PERFORMANCE

IP = INDEPENDENT PERFORMANCE

O = OBSERVATION

P=POOR S= Satisfactory E= Excellent GOOD

Assignments of Clinical Microbiology



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1 Case interpretation (2)

2 SOP (2)

Assignments of Total Quality Management in clinical laboratory



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1 Evaluation

2 Inventory

3 Pre and post analytical

4 QC charts (10)

5 EQAS (10)

Assignments of Medical Research


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1 Designing research method

2 Preparation of informed consent

3 Statistical analysis of results

Assignments of Therapeutic Drug Monitoring



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1 SOP (TDM)

2 SOP(clinical toxicology)

3 SOP(toxic metals)

Assignments of Infection control



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1 Audit of hand washing in the

hospital(2)

2 Audit of personal protective

equipments(2)

Assignments of Blood Transfusion



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5 - Log book preview

The candidate logbook will be reviewed and skills performed summarized by

diagnosis groups during the semester evaluation and at the end of the course in the

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table below. This reflects the number of activity done by category. The results of

this review will be totaled in the summary chart below.

Summary

Semester 1st 2

nd 3

rd 4

th Total

Activity No No No No

Conferences attendance

Thesis attendance

Case presentation

Scientific meetings

Practical skills ( clinical chemistry):

Weighing and preparing standards

Acid preparation

Specimen Collection and processing

Spectrophotometers

End point assays

Kinetic assays

Urine analysis

Semen analysis

Assignments ( clinical chemistry):

Practical skills ( lab. hematology):

Hemoglobin curve

Hb measurement

Manual WBC count

Manual platelet count

Blood film preparation & Leishman

staining

Manual differential count

Automated CBC

Reticulocyte preparation & count

PCV by microhematocrit

ESR by Westergren method

Coombs test (direct)

Coombs test (indirect)

PT determination

PTT determination

Blood grouping (slide /tube methods)

Cross matching (slide /tube methods)

Bone marrow aspirate interpretation

Bone marrow iron staining

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Peroxidase staining

BM aspiration

Flow cytometric immunophenotyping

Cytogenetic analysis

Coagulation factor assay

Platelet aggregation

PFA 100

G6PD assay

Hb electrophoresis

Blood grouping (column

agglutination)

Cross matching (column

agglutination)

Blood component separation

/preservation

Assignments(Lab.hematology)

Practical skills (clinical imm.)

Elisa qualitative

Elisa quantitative

Autoantibodies (ANA)

Autoantibodies (DNA)

Autoantibodies (ATA)

Lymphocyte separation

Phagocytosis assay

PCR

HLA (microcytotoxicity)

HLA (PCR)

Immunofixation

Assignments ( Clinical Imm.)

Practical skills (Cinical Micro.):

Handling microscopy

Wet film preparation& examination

Preparation & fixation of stained

films

Preparation of Gram stained films

&differentiation between G+ve &

G-ve organisms

Preparation of ZN stained films &

identification of AFB

Identification of different culture

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media & its important constituents

Streaking of solid agar plates

Streaking of solid agar plates for

semiquantitative colony count

Streaking of a tube of broth media

&slant agar

Identification of isolates on different

culture media

Identification of anaerobic culture &

transport media, gas generating packs

& gas pack jar

Preparation of Gram stained films

&differentiation between

G+ve & G-ve organisms

Preparation of ZN stained films &

identification of AFB

Identification& streaking of different

culture media

Identification& streaking of different

biochemical reaction media

Interpretation of different organisms

by different biochemical reactions

Preparation & interpretation of

antibiotic sensitivity

Interpretation of Widal & Brucella

agglutination tests

Interpretation of haemagglutination,

haemagglutination inhibition,

antistreptolysin O titre ELISA tests

Dealing with urine specimens

Dealing with genital specimens

Dealing with stool specimens

Dealing with pus &wound specimens

Dealing with blood culture specimens

Dealing with CSF& body fluids

specimens

Dealing with upper respiratory tract

specimens

Dealing with lower respiratory tract

specimens

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Preparation of KOH wet films for

mycological specimens

Preparation of germ tube test

Preparation of slide culture technique

Identification of anaerobic culture &

transport media, gas generating packs

& gas pack jar

Assignments (Clinical Micro.)

Assignments of elective courses:

-Total quality management in

clinical lab.

-Medical research

- Therapeutic Drug Monitoring

(TDM) and Clinical Toxicology

-Infection control

-Blood transfusion

Supervisor signature

VII - HEAD OF DEPARTMENT APPROVAL FOR THE EXAM ENTRY

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VIII – EVALUATION FORMS

Evaluation Form (at the end of each semester) To be completed at -------------------------------------

Candidate

Supervisor

Location

Aim of training

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Agreed educational objectives and timescale in which objectives should be

achieved.

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Comments by Candidate

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Comments by Supervisor

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Date of next meeting

Signed by candidate

Signed by Supervisor

Date

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Candidate

Supervisor

Location

Aim of training

__________________________________________________________________

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__________________________________________________________________


achieved.

__________________________________________________________________

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Signed by candidate


Date

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Candidate

Supervisor

Location

Aim of training

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__________________________________________________________________


achieved.

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________


__________________________________________________________________

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__________________________________________________________________

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Signed by candidate


Date

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Candidate

Supervisor

Location

Aim of training

__________________________________________________________________

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__________________________________________________________________


achieved.

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Signed by candidate


Date

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Degree Program Evaluation Form By The Candidate

To be completed at the end of your degree.

Please consider each pair of statements and decide which most clearly reflects your

view and tick one box or answer the question.

I. Individual Information

1. Are you a graduate of ASU?

yes no to some degree

2. Year and semester when studies began:

II. General Questions

1. What are the advantages/disadvantages of the general study environment at the

University ASU?

2. What were your expectations when you applied to the degree?

3. Do you feel that the degree program prepares you well for your future studies or

employment according to the demands and expectations of those institutions?


4. Has the time limit of the program (two or three academic years) caused you any

difficulties or inconveniences?


III. Structure of Degree Program

1. Did you receive enough guidance in planning your study schedule in the

beginning of the program?


2. What were the main difficulties in the planning of your study schedule?

3. What is your general opinion on the structure of the degree program?

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4. In your opinion, does the degree program offer a good balance of lectures,

seminars, conferences, and book exams?


a) General Studies

i) Do you feel that you have received enough guidance on academic writing?


ii) Do you feel that you have acquired sufficient knowledge on research skills (eg.

quantitative and qualitative research methods)?


b) Courses

i) Have you had some special difficulties in completing some of the courses?

Please specify.

ii) Has there been a sufficient variety of courses offered for your optional studies?


iii) Have you received enough guidance for the preparation of your thesis?


IV. Concluding Points

1. Did the degree program meet your expectations?


2. What aspects of the degree program do you particularly like?

3. What aspects of the degree program do you particularly dislike?

4. What are your suggestions on how to improve the program?

Thank you!

Diploma in Clinical Pathology - Ain Shams...

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