Digestomica delle proteine: piattaforme integrate ... - CNR · Digestomica delle proteine:...
Transcript of Digestomica delle proteine: piattaforme integrate ... - CNR · Digestomica delle proteine:...
“Digestomica delle proteine: piattaforme integrate per l’identificazione di peptidi
bioattivi e funzionali derivati dagli alimenti”
Dr. Gianluca PicarielloIstituto di Scienze dell’Alimentazione (ISA) - CNR, Avellino
Bologna, 21 ottobre 2016
food protein
food-derivedpeptides
inactive
active (or immunogenic??)
FermentationEnzymatic/acid hydrolysisRipening (proteolysis) Gastrointestinal digestion
MILK DERIVED BIOACTIVE PEPTIDES
Caseins (78-80%)(phosphoproteins)
αs1-caseinαs2-caseinκ-caseinβ-casein
Whey proteins (18-20%)
α-lactalbuminβ-lactoglobulinlactoferrin
Bioactive peptides
Milk-derived bioactive peptides
Digest from the entire gliadin fractionfrom hexaploid wheat
Digest from whole casein and whey protein fractions
Complexity of the food matrix
Including an intestinal (jejunal)step of simulated digestion villi BBM
Absorption of bioactive peptides (bioavailability)
A few peptides (potentially immunogenic ratherthan bioactive) at very low amount detected in the basolateral chamber
Gastro-duodenal digests of milk proteinsapplied to the apical chamber
Case studies: milk, wheat, ancient cereals, meat, tree nuts
Mature milk (2-4 weeks after term delivery) from 12 healthy donors:- Six mothers observing a strict milk (and dairy-products) free diet- Six mothers drinking a cup of bovine milk daily and eating dairy products
Peptide from BOVINE MILK Protein of origin (position)Uniprot
protein ID
Number of times
identified
Motherson milk-free diet
(N=6)
Milk-drinking mothers
(N=6)
YVEELKPTPEGDL β-lactoglobulin (42-54) P02754 2 - 2
YVEELKPTPEGDLEIL β-lactoglobulin (42-57) P02754 1 - 1
SDIPNPIGSENSEKTTM*PLW αs1-casein (180-197) P02662 1 - 1
Aug 2016
Concluding remarks
Many supposed food-derived peptides are not stable to gastrointestinal digestion
Stability to digestion should be assessed to confirm a possible (local or systemic)activity
The identification of digestion stable peptides should be performed on real foodsystems, using physiologically realistic digestion/absorption models
The characterization of the «digestome» requires specialized and specifically designedanalytical methodologies
Analysis of the protein "digestome" : needs of integrated approaches
bioinformatics
food
fractionation
immunoreactivity
cell biology
perspective: metagenomics