8/14/2019 Different Datura Species By Momer IonsClub.Com Team in SIUST University

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8/14/2019 Different Datura Species By Momer IonsClub.Com Team in SIUST University

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Hyoscyamine: a poisonous alkaloid that resembles atropine. Use: dilates blood

vessels, sedative , prevents or controls spasms, used as antidote to the toxicity of

cholinergic compounds and decrease the secretion ( Upper and lower respiratory

tract) before surgery.

The objective of this laboratories is to isolate these active constituents individually

using many techniques, identify them using IR and UV spectroscopy methods and

finally to determine their concentration in each species.

Procedure:

Discussion:

We use 90% Ethanol in the refluxing process to isolate free alkaloids ( in

ethanol) and their salts in water.

We use the reflux in the extraction process to the following:

1-To prevent the evaporation of ethanol from the bottle during its boiling ,i.e

the reflux condense the vapors of Ethanol to get back into the flask

2-The metal leaves constituents are heat-stable.

We evaporate the ethanol to 50 ml in order to concentrate the solution and use

the available glass waresWe add 2% HCl to convert the free alkaloids to their salt the use of 2% not more

to prevent the destruction of alkaloids.

The addition of ammonia hydroxide to convert the salt to their alkaloids in order

to extract them in Chloroform.

We add the ammonia hydroxide to the evaporated filtrate after cooling to

prevent the evaporation of the ammonia hydroxide.

We extract finally the filtrate in two halves with chloroform to enhance the

extraction which is proved mathematically according to coefficient factor

equation.

We use TLC to determine how many compounds we have by spraying the plates

with Dragendroff's reagent. The mobile phase used is ( actone: water: ammonia

(90:7:3)) and the colored is between red to orange.

KeyWord:

Datura Inoxia Stramonium metal quantitative qullitative Dragendroff Alkaloid Hyoscine

scopolamine HyosciamineSIUST IonsClub.Com