J ALLERGY CLIN IMMUNOL

FEBRUARY 2012

AB40 AbstractsSATURDAY

151 Nutritional Status and Bone Metabolism in Children WithAtopic Dermatitis

V. R. A. Penterich, A. C. Yang, R. M. R. Pereira, J. Kalil, F. F. M. Castro;

School of Medicine - University of Sao Paulo, Sao Paulo, BRAZIL.

RATIONALE: Atopic dermatitis (AD) is an inflammatory allergic skin

disease that often requires topical glucocorticoid therapy (GC). To

determine the impact of AD on nutritional status and bone parameters in

children with moderate/severe AD compared to control group.

METHODS: Forty-nine children with AD (4-12 years) and 48 healthy

controls were evaluated regarding nutritional status (height/age z-score,

weight/age z-score and BMI z-score), disease activity and severity, topical

GC use, and bone parameters. Bone mineral content (BMC), bone mineral

density and z-score were measured at lumbar spine and total femur by

DXA. Laboratory parameters analyzed were calcium, alkaline phospha-

tase, CTX, 25OHD and cortisol.

RESULTS: Children with AD compared to control group presented with

lower height for age z-score (P50.007), lower BMC at lumbar spine

[16.5(6.4) vs. 19.8(8.3)g, P50.027] and total femur [12.2(4.0) vs. 14.2(5.0)

g, P50.029]. Bone resorption marker (CTX) was lower in children with

AD compared to healthy controls [1.36(0.59) vs. 1.67(0.79)ng/mL,

P50.026] and a tendency of lower bone formation marker (alkaline

phosphatase) was also observed [228(75.3) vs. 255(70.7)ng/mL,

P50.074]. Children with AD presented inferior levels of serum cortisol

in comparison to healthy group [9.06(4.8) vs. 10.57(4.9), P50.061].

CONCLUSIONS: Lower lumbar and total femur BMC and lower bone

turnover found in children with AD is probably due to a chronic GC use.

152 Dietary Intervention With Specific Non-digestibleOligosaccharide Mixtures Is Effective During Induction OfMurine Cow'S Milk Allergy But Not Suitable As Treatment InThe Same Allergic Disease Model

P. V. Jeurink1,2, J. Kerperien2, T. M. D. Wehkamp1,2, E. C. A. M. van

Esch1,2, A. Rijnierse1,2, G. A. Hofman2, L. E. M. Willemsen2, J. Gars-

sen1,2, L. M. J. Knippels1,2; 1Danone Research - Centre of Specialised Nu-

trition, Wageningen, NETHERLANDS, 2Utrecht Institute of

Pharmaceutical Sciences, Utrecht, NETHERLANDS.

RATIONALE: In this study, the effects of dietary supplementation with

specific non-digestible oligosaccharides (prebiotics) either as single com-

ponents or in mixtures on the outcome of the murine allergic response

when provided before and during oral sensitization with whey were

investigated. In addition, the feasibility to treat allergic mice with these

prebiotics was addressed.

METHODS: Mice were fed diets containing 1% single prebiotics,

combinations of scGOS (short chain galacto-oligosaccharides), lcFOS

(long chain fructo- oligosaccharides) and/or pAOS (pectin-derived acidic

oligosaccharides) or a control diet. The diets were provided two weeks

prior to and during oral whey-sensitization (prevention) or for four weeks

starting one week after the last sensitization (treatment). The acute allergic

skin response (ITH) and antigen-induced anaphylaxis (AiA) were deter-

mined one hour after intradermal whey-challenge. Whey-IgE/G1/G2a and

mouse mast cell protease-1 (mMCP-1) were determined in serum.

RESULTS: In the whey-sensitized preventive intervention groups, only

combination of scGOS/lcFOS (9:1) and scGOS/lcFOS/pAOS (9:1:2)

strongly reduced both ITH (p<0.001) and AiA compared to controls.

scGOS/lcFOS/pAOS was most effective, whereas single components were

not. mMCP-1 tended to be reduced in thewhey-sensitizedmice fed scGOS/

lcFOS(/pAOS), although the whey-IgE/G1/G2a remained high. In the

treatment groups, allergic disease was confirmed prior to the diet

intervention by presence of whey-IgE, ITH and AiA. Four weeks of

dietary intervention with scGOS/lcFOS(/pAOS) did not alter the allergenic

response compared to controls.

CONCLUSIONS: Dietary supplementation with scGOS/lcFOS(/pAOS)

provided as prevention reduced the allergic effector response. However,

these diets were reluctant to treat allergy in the chosen intervention

protocol in the murine model of IgE-mediated hypersensitivity.

153 Different Timings of Prenatal or Postnatal Tobacco SmokeExposure have Different Effects on The Development ofAtopic Eczema/Dermatitis Syndrome (AEDS) during Infancy

M. Shinohara1,2, H. Saito3, K. Matsumoto4; 1National Shimosizu Hospi-

tal, Department of Pediatric Allergy and Clinical Research, 934-5 Shika-

watashi, Yotsukaido-City, Chiba, JAPAN, 2Kochi University, Department

of Pediatrics, Kochi, JAPAN, 3National Research Institute for Child

Health and Development, Department of Allergy & Immunology, 2-10-

1 Okura Setagaya-ku Tokyo, 157-8535 Japan, JAPAN, 4National Research

Institute for Child Health and Development, Department of Allergy & Im-

munology, -10-1 Okura Setagaya-ku Tokyo, 157-8535 Japan, JAPAN.

RATIONALE: Tobacco smoke is an important environmental factor for

allergic diseases. Prenatal tobacco smoke exposure affects both fetal lung

and immune function with increased subsequent risk of asthma and

respiratory infection in infancy. About the skin, tobacco smoke increases

the prevalence of wrinkling, skin cancer, psoriasis and systemic lupus

erythematosus. The influence of tobacco smoke for the development of

atopic eczema/dermatitis syndrome (AEDS) is controversial and when is

the critical period unclear. Therefore, we investigated whether the different

periods of maternal tobacco smoke exposure have different impacts on the

development of AEDS, or not.

METHOD: A total of 1,436 infants (age 2.0 -18.0months) was enrolled in

this cross-sectional questionnaire survey. Family history of allergic

diseases, number of older siblings, prenatal and postnatal maternal tobacco

smoke exposure, development of physician-diagnosed AEDS and so forth

were asked by a self-writing questionnaire. An adjusted logistic regression

model was analyzed by STATA software.

RESULTS: The recovery rate was 97.2% (1436/1476). The prevalence of

AEDSwassignificantly increased in the infantswith tobacco smokeexposure

during third trimester pregnancy (aOR, 6.146; 95% CI, 1.282 - 29.453) than

those without tobacco smoke exposure. The prevalence of AEDS was no

significant differences between the infants with tobacco smoke exposure

during first trimester pregnancy, during the first 6 months of life and after 6

months after birth than those without tobacco smoke exposure, respectively.

CONCLUSIONS: Maternal tobacco smoke exposure during third trimes-

ter pregnancy might have the strongest impact effect on increasing the

development of AEDS in their offspring.

154 Asthma and Physical Activity in Adolescents: A CrossSectional Analysis

A. L. Leyton, A. P. Baptist; University of Michigan, Ann Arbor, MI.

RATIONALE: The prevalence of asthma and obesity has increased during

the past decades. It is unclear if the increasing prevalence is causal as there

is conflicting evidence regarding factors that affect physical inactivity

among adolescents. This project assessed factors correlated with physical

activity levels in adolescents.

METHODS: An anonymous cross-sectional survey was distributed to

students attending two public high schools. The survey consisted of the

Physical Activity Questionnaire for Adolescents (PAQA), demographic

questions, and modified versions of the Juniper Asthma Control Test and

the Juniper Pediatric Asthma Quality of Life Questionnaire.

RESULTS: Among all students (n5181) higher average PAQA scores were

significantly correlatedwith male gender (p50.005), lower BMI (p50.008),

never smoking (p50.021), less TV hours per week (p50.001) and less

computer hours per week (p50.000). There was no significant correlation

between average PAQAscores and a previous or current diagnosis of asthma.

Among students with current asthma (n526), higher average PAQA scores

trended toward a significant correlation with later age of asthma diagnosis

(p50.078) and higher family income (p50.085) while lower family income

(p50.027), increased smoke exposure (p50.039), and higher BMI

(p50.048) were significantly correlated with worse asthma control.

CONCLUSIONS: Among adolescents, demographic characteristics such

as BMI, hours spent engaging in sedentary behavior, and smoking have a

greater impact on average physical activity score than the presence of

asthma. Among asthmatic adolescents, interventions targeting obesity and

smoke exposure appear to be important in order to improve control.