Développement de méthodes rapides pour l'authentification des produits biologiques

Sûreté alimentaireQuelles innovations pour

la maîtrise des contaminants et l'authentification des produits

agricoles et alimentaires ?

> 13 & 14 novembre 2013 > Montpellier SupAgro INRA

www.rencontres-qualimediterranee.fr

Kristian HOLST LAURSEN

Faculté des sciences, Section plantes & sol,

Université de Copenhague (Danemark)

Fast methods for authentication of organic basedfood

- presentation of the "AuthenticFood" project

Laursen, Kristian Holst, Dr., University of Copenhagen

Organic versus conventional plant production


Organic Conventional

Pesticides No Yes

Synthetic fertilizers No Yes

Organic fertilizers Yes Yes

Fertilizer amount Lower or similar Higher or similar

Crop rotations Longer Shorter

Yields Lower or similar Higher or similar

Product price Higher Lower

Organic agricultural land in Europe


Source: www.fibl.org

Credibility in the organic market- Certifiction does not protect consumers


Research objectives of AuthenticFoodTo develop and test a portfolio of analytical techniques forauthentication of organic plant foods

• Ionomics• Stable isotopes• Metabolomics• Pesticide screening

To evaluate the techniques in corporation with Europeaninspection and certification bodies

• Ecocert - France• BIOS - Italy• DVFA - Denmark


AuthenticFood partners


KU‐ScienceFOOD‐DTUDVFA

FERA

AIABFEM‐IASMABIOS

EurofinsEcocert

Bioforsk UHEL

RIKILT

ICT

GL Centre Unika

LIAEFP7 ERA‐NET project16 partners11 European countriesProject leader: Søren HustedPeriod: 2011‐2014

AuthenticFood workpackages

WP1: Project coordinationWP2: Sample production and distributionWP3: Multi-elemental analysis, 31P (NMR)WP4: Stable isotopes, δ2H, δ13C, δ15N, δ18O, δ34S, nitrate

and sulfate isotopes, δ15N in amino acids WP5: Metabolomics, phenolic content, volatile analysis, fatty

acids, sensory profilesWP6: Pesticide residuesWP7: ChemometricsWP8: Results dissemination


Biological cases


Experimental factors• Organic/conventional• Plant species• Plant variety• Growth season• Geography• Processing

AuthenticFood project status


Essential plant nutrients


The periodic table

H

He

Li

Be B

C

N

O

F

Ne

Na

Mg Al

Si

P

S

Cl

Ar

K

Ca

Sc

Ti

V

Cr

Mn

Fe

Co

Ni

Cu

Zn

Ga

Ge

As

Se

Br

Kr

Rb

Sr

Y

Zr

Nb

Mo

Tc

Ru

Rh

Pd

Ag

Cd

In

Sn

Sb

Te

I

Xe

Cs

Ba

La

Hf

Ta

W

Re

Os

Ir

Pt

Au

Hg

Tl

Pb

Bi

Po

At

Rn

Fr

Ra

Ac

Rf

Db

Sg

Bh

Hs

Mt

Uun

Uuu

Uub

Lanthanider Ce

Pr

Nd

Pm

Sm

Eu

Gd

Tb

Dy

Ho

Er

Tm

Yb

Lu

Actinider Th

Pa

U

Np

Pu

Am

Cm

Bk

Cf

Es

Fm

Md

No

Lr

Non‐mineral Macronutrient Micronutrient

Basically all trace elements are found in plants – The Plant Nano-nutrients


Mass (m/z)

0 50 100 150 200 250

Ion

inte

nsity

(cou

nts

s-1)

1e-1

1e+0

1e+1

1e+2

1e+3

1e+4

1e+5

1e+6

1e+7

1e+8

1e+9

1e+10

Ni reference line

B

Mg K

Mo

Mn

Essential Nutrients (11B - 95Mo)Background subtracted spectrum

Mass (m/z)

0 50 100 150 200 250

Ion

inte

nsity

(cou

nts

s-1)

1e-1

1e+0

1e+1

1e+2

1e+3

1e+4

1e+5

1e+6

1e+7

1e+8

1e+9

1e+10

Co

Cd

Se

Mass scan 7Li – 238U

Multi-elemental plant tissue analysis


Stable isotope analysis


99.63% 0.37%

”light” isotope ”heavy” isotope

14N 15N

10001

14

15

14

15

15

air

air

sample

sample

sample

NN

NN

N

Units o/oo

Multi-isotopic analysis can reveal the fertilization history of plants


δ15N (bulk tissue)

δ18O (nitrate)

Low δ15N • Synthetic• Legume

High δ15N • Slurry• Manure• Compost

Low δ18O• Legume

High δ18O• Synthetic

How was I fertilized?

Conclusions


Metabolites

Trace elements

Pesticide residues

Compund‐specificisotopes

Stable isotopes


Thank you for your attention!

www.coreorganic2.org/authenticfood

[email protected]

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Développement de méthodes rapides pour l'authentification des produits biologiques

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