Developmental stage-specific biphasic roles of Developmental stage-specific biphasic roles of WntWnt signaling signaling

in cardiomyogenesis and hematopoiesisin cardiomyogenesis and hematopoiesis

(Atsuhiko T. Naito et al, 2006, Development)

Wnt signalings in embryonic development

Members of the Wnt and Tgfβ/Bmp families play critical roles in anterior-posterior patterning

(Yamaguchi TP, 2001, Curr Biol)

Zygotically expressed wnt8 may be involved in specifying or patterning ventral mesoderm.

(Gregory M Kelly, 1995, Development)

Interactions between Wnt and Vg1 signalling pathways initiate primitivestreak formation in the chick embryo

(Isaac skromne, 2001, Development)

Wnt signaling AP Axis patterning, primitive streak formation, mesoderm induction

Wnt signalings that related in development of mouse embryo

(Kimelman D, 2006, nature)

Development of Mouse embryo

(Yamaguchi TP, 2001, Curr Biol)

Many controversies about Wnt signaling in heart formation

In chick embryo, Wnt signals from the neural tube block ectopic cardiogenesis

(Eldad Tzahor, 2001, Genes & Dev)

Dkk-1 can induce heart-specific gene expression in noncardiogenic mesoderm of Xenopus laevis

(Valerie A., 2000, Genes & Dev)

Overexpression of Wg using heat shock promoter increases the amount of heart formation in Drosophila

(Maiyon Park, 1996, Dev Biol)

Heart development in Drosophila requires the segment polarity gene wingless(Wg).

(Wu X, 1995, Dev Biol)

(Eldad Tzahor, 2001, Genes & Dev)

Overexpression of Wnt-1, 3 causes on reduction of cardiomyocyte

In chick embryo

Overexpression of Wg causes on abundance of cardiomyocyte

In gastrula of Drosophila

about a fourfold increase of heart precusor cell occurs in hswg embryos upon heat-shock for 30min at 37.5℃

(Park M, 1996, Dev Biol)

Hanging drop method

Wnt Signaling promotes Cardiomyogenesis During Early Stage of

ES Cell Differentiation.

Wnt Signaling promotes Cardiomyogenesis During Early Stage of

ES Cell Differentiation.

Wnt Signaling inhibits Cardiomyogenesis During Late Stage of

ES Cell Differentiation.

Wnt inhibits cardiomyocyte differentiation in the late phase by

suppressing BMP signaling.

Modulation of Wnt Signaling Enables Efficient Induction of Cardiomyocytes from ES Cells.

Wnt signals exhibit developmental stage-specific, biphasic, andantagonistic effects on cardiogenesis and hematopoiesis.

(Yamaguchi TP, 2001, Curr Biol)