Development of Local and Scanning Probe Techniques Heinrich Hoerber NanoBioPhysics University...
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![Page 1: Development of Local and Scanning Probe Techniques Heinrich Hoerber NanoBioPhysics University Bristol.](https://reader035.fdocuments.in/reader035/viewer/2022062500/56649d385503460f94a11909/html5/thumbnails/1.jpg)
Development of Development of Local and Scanning Local and Scanning Probe TechniquesProbe Techniques
Heinrich HoerberNanoBioPhysicsUniversity Bristol
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200 nm
Haberle, W., J. K. H. Horber, and G. Binnig, 1991, Journal of Vacuum Science & Technology B 9, 1210
Combining AFM and Combining AFM and optical microscopyoptical microscopyCombining AFM and Combining AFM and optical microscopyoptical microscopy
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AFM image sequence of a pox virus release at the end of a microvillus
EM image by Stokes 1976
Scanning fast - making Scanning fast - making moviesmoviesScanning fast - making Scanning fast - making moviesmovies
Horber, J.K.H., et al., 1992, Scanning Microscopy 6, 919
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Horber, J. K. H., W. Haberle and
B. Sakmann, 1995 Biophysical Journal 68, 1687
Combination with Combination with electro-physiological techniqueselectro-physiological techniquesCombination with Combination with electro-physiological techniqueselectro-physiological techniques
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Koitschev, A., S. Fink, U. Rexhausen, K. Loffler, J. K. H. Horber, H. P. Zenner, J. R. Ruppersberg, and M. G. Langer, 2002, Hno 50, 464-469
AFM in hearing AFM in hearing researchresearchAFM in hearing AFM in hearing researchresearch
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Cantilever Cantilever developmentdevelopmentCantilever Cantilever developmentdevelopment
IBM Research Laboratory RueschlikonM. Despont, G. Binnig, P. Vettiger and C. Gerber
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Heat flow between Heat flow between cantilever and substratecantilever and substrate
Heat flow between Heat flow between cantilever and substratecantilever and substrate
Heat transfer throughcantilever arms (~4 mW)
Cantilever with heater
through tip in contact 50-500 nW
Through air to substrate (10-20 μW)
50-5000C
ΔR/R: 10-6–10-5/nm
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2 μm
Lipid vesicles with reconstituted membrane proteins (SNAP25, B.Jena)
2 μm1 μm
SiC GaN
Metal connection on a storage chip structure underneath a SiO2 layer(Zarlink)
Cut through a transistor structure(M. Kubal)
Heat conductivityHeat conductivityHeat conductivityHeat conductivity
Haeberle, W, Pantea, M & Hoerber, JKH. 2006, Ultramicroscopy, 106 (8-9), 678
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100 m 1 m
length 260 m, width 1 m, thickness 200 nm, spring constant 0.03 pN/nm
Smaller cantileversSmaller cantileversSmaller cantileversSmaller cantilevers
James Vicary
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AFM with the cantilever AFM with the cantilever verticalverticalAFM with the cantilever AFM with the cantilever verticalvertical
Massimo Antognozzi, Arturas Ulcinas
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Maximal cantilever bending
Molecular Motor Molecular Motor movementmovementMolecular Motor Molecular Motor movementmovement
Massimo Antognozzi, Tim Scholz
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0.5 nm
200 nm
200 μm
0.02 N/m
Florin, E. L., A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, 1998, Applied Physics A-Materials Science & Processing 66, S75
Spatial resolution Spatial resolution ~ 1nm~ 1nmTime resolution ~ 1Time resolution ~ 1μμsecsec
PPhotonic hotonic FForce orce MMicroscopyicroscopyPPhotonic hotonic FForce orce MMicroscopyicroscopy
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Agarose polymer networkAgarose polymer network
Tischer, C. et al., 2001, Appl. Phys. Lett. 79, 3878
Thermal fluctuation Thermal fluctuation imagingimagingThermal fluctuation Thermal fluctuation imagingimaging
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Membrane diffusion 2D / Membrane diffusion 2D / 3D3DMembrane diffusion 2D / Membrane diffusion 2D / 3D3D
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Confining potential map
Viscosity map
Interaction map of an LDL Interaction map of an LDL receptorreceptorInteraction map of an LDL Interaction map of an LDL receptorreceptor
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Parallelisation of Parallelisation of PFMPFMParallelisation of Parallelisation of PFMPFM
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Producing many Producing many independent independent focused laser beamsfocused laser beams
Producing many Producing many independent independent focused laser beamsfocused laser beams
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Nano-particles crossing Nano-particles crossing the cell membranethe cell membrane
Nano-particles crossing Nano-particles crossing the cell membranethe cell membrane
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Types of nano-Types of nano-particlesparticlesTypes of nano-Types of nano-particlesparticles
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Gold nano-particles Gold nano-particles
interacting with interacting with lightlight
Gold nano-particles Gold nano-particles
interacting with interacting with lightlight
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Nearfield fluorescence Nearfield fluorescence excitationexcitationNearfield fluorescence Nearfield fluorescence excitationexcitation
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Raman Spectroscopy
Chemical sensingChemical sensingChemical sensingChemical sensing
Surface EnhancedRaman Spectroscopy
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Nano-Sensors (Scanning Probe Microscopies)
Nano-toxicology
Nano-medicine (markers, drug delivery)
Nano-particles - areas of interestNano-particles - areas of interest
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