Determine new application spaces in the sequencing platform using visions systems
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Determine new application spaces in the sequencing platform using visions systems Jessica Perez Summer Research Program in Genomics Massachusetts Institute of Technology Summer 2007
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Determine new application spaces in the sequencing platform using visions systems. Jessica Perez Summer Research Program in Genomics Massachusetts Institute of Technology Summer 2007. Outline. Approach and Goals Vision Systems Potential Application Spaces Demonstrated Application Spaces. - PowerPoint PPT Presentation
Transcript of Determine new application spaces in the sequencing platform using visions systems
Determine new application spaces in the sequencing platform using visions systems
Jessica PerezSummer Research Program in GenomicsMassachusetts Institute of TechnologySummer 2007
Outline
Approach and Goals Vision Systems Potential Application Spaces Demonstrated Application Spaces
Project Overview
Approach: use Cognex Smart Sensor and Intellect 1.4.0 software as a model to demonstrate the capabilities of vision systems
Goals: Explore capabilities of software Look for application spaces Take application and prove principal
Vision Systems
Software Capabilities
Lighting
Post Processing
Software Capabilities
Blob Identification
1D Bar Scanning
Flexible Flaw Detection
User Interface
Result Table
Tool Box Viewing Screen
Blob Identification
Darker Objects Meet Parameters
Lighter Objects FAILED
IDENTIFICATION CRITERIA
AreaAverage Intensity
1D Code Reader
Numerical Code Read
PASS
Flaw Detection
Model
PARAMETERS
Blob Color
Area
Perimeter
Additional Tests
Counting Filtering
Measurement
2D String Reader
Optical Character Recognition Reader
TOOLS
Lighting
Answer.com
Filtering Intensity
Geometry
Common Lighting Techniques
Bright Field Dark Field Back Lighting
From Vision Light Tech
Post Processing
MatLab Histogram Filter
Before After
Potential Application Spaces
Process: Solexa ScreeningConsumer: Glass Tubes
Process: Solexa Screening
Quality Control Check in the Solexa Sequencing Process
ClustersFlowcells
Proof of Principle
Blobs represent clusters of DNA
Consumer : Test Tube
DNA
Sonic Waves
Probe
Sonication
Backlighting
BEFORE
AFTER
Detection of Dimensions and Shape of Transparent Objects
Images by Keyence-Engineering Knowledge
Setup Components
LED Strobe Light
Magnification Lens
Diffuser
Setup
Mask
Tubes
Camera
Strobe Light
Finish Product
Distance to be measured
Tube under Backlighting
Conclusion
Vision systems have unlimited potential Implementation of vision systems will improve
the efficiency at various stages of the sequencing process.
Acknowledgements
Technology Development Group Eva Markiewicz Shailendra Yadav Filip Rege Andrew Barry Todd Sparrow Tracey Honan Pat ___
Directors of Education Shawna Young Dedric Carter
Eric Lander
Broad Institute
