Design of a microfeatured PGSM scaffolds for corneal ...
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Design of a microfeatured PGSM scaffolds for corneal regeneration Iris Cristina Becerril Rodriguez, Illida Ortega Asencio, Sheila MacNeil, Frederik Claeyssens [email protected] Introduction Results Methods Aim POLYMER SYNTHESIS SCAFFOLD DESIGN SCAFFOLD SYNTHESIS 1 2 3 Problem PGS PGSM PGSM crosslinking Conclusion PGSM shows tuneable degradation, mechanical and physical surface properties, making it an ideal candidate for soft-tissue applications. Previous results obtained from our research group suggest that PSGM-based scaffolds are not toxic. Detailed in vitro study with LEC’s and LF’s currently in progress STEREOLITHOGRAPHY/MOULDING • Biocompatible • Biodegradable • Elastomer • Transparent • Young´s modulus 0.0250 – 1.2 MPa) Cornea 0.29 MPa Glycerol Sebacic acid 20% 30% 40% 50% Deshpande,et.al (2009). Development of a Surface modified contact lens for the transfer of cultured limbal epitelial cells to the cornea for ocular Surface diseases. Tissue engineering. 15. 2889 – 2902 Li, Y., et.al.(2012). Synthesis, characterization and properties of biocompatible poly (glicerol sebacate) pre-polymer and gel. Polym Int. 62. 534 – 547. Loh, X., et.al. (2015). Poly (glicerol sebacate) biomaterial: synthesis and biomedical applications. Journal of Materials Chemistry. 3. 7641 – 7652. May et.al. (2011). Chapter 49 Regenerative Medicine in the Cornea. Principles of Regenerative Medicine. Elsevier. 911- 924. Ortega, Iet.al. (2012). Combined microfabrication and electrospinning to produce 3-D architectures for corneal repair. Acta biomaterialia. 9. 5511 – 5520. Acknowledgements Dr. Frederik Claeyssens Dr. Ilida Asencio Prof. Sheila MacNeiland The University of Sheffield National Science and Technology Council in Mexico (CONACyT) scholarship Armourers and Brasiers companys grant Learning societys funding Characterization • Chemical • FTIR • GPC (Mw 23,000 g/mol) Scaffold design Scaffold synthesis Visual impairment of the cornea is the 4th global cause of blindness 7 million of people go blind every year 180 million of people are blind or visually disabled worldwide Corneal vascularization cause blindness in 8 million people (10% of total blindness) worldwide each year Causes of LSCD: • Hereditary: Aniridia Stevens Johnson´ s syndrome • Acquired: Thermal/ chemical burns UV radiation Poly (glycerol sebacate) methacrylate Poly (glycerol sebacate) Developed a safe and efficient corneal cell carrier. This carrier should mimic the anatomical structure of the limbus to provide physical protection to the cells and ensure their survival 4 . PGS-M can be structured into complex designs models that mimic the curvature of the native cornea. PGS-M is a biocompatible material suitable for corneal regeneration Take home message • The cornea has limbal stem cells (LSCs) that are in niches in the limbus • LSCs are responsible for the corneal integrity and healthy balance • LSCs are a proliferative barrier between the corneal epithelia and the conjunctiva and its blood vessels • Limbal stem cells deficiency (LSCD) causes that this barrier breaks down, allowing conjunctival cells to move onto the cornea causing vascularization. • LSCD produce scar tissue, causing pain, blushing and blindness
Transcript of Design of a microfeatured PGSM scaffolds for corneal ...
Design of a microfeatured PGSM scaffolds for corneal regeneration
Iris Cristina Becerril Rodriguez, Illida Ortega Asencio, Sheila MacNeil, Frederik Claeyssens
Introduction
Results
Methods
Aim
POLYMER SYNTHESIS SCAFFOLD DESIGN
SCAFFOLD SYNTHESIS
1 2
3
Problem
PGSPGSM
PGSM crosslinking
Conclusion
PGSM shows tuneable degradation, mechanical and physical surface properties, making it an ideal candidate for soft-tissue applications.
Previous results obtained from our research group suggest that PSGM-based scaffolds are not toxic.
Detailed in vitro study with LEC’s and LF’s currently in progress
STEREOLITHOGRAPHY/MOULDING
• Biocompatible• Biodegradable• Elastomer• Transparent• Young´s modulus 0.0250 –
1.2 MPa) Cornea 0.29 MPa
Glycerol
Sebacic acid
20% 30%
40% 50%
Deshpande,et.al (2009). Development of a Surface modified contact lens for the transfer of cultured limbal epitelial cells to the cornea for ocular Surface diseases. Tissue engineering. 15. 2889 – 2902 Li, Y., et.al.(2012). Synthesis, characterization and properties of biocompatible poly (glicerol sebacate) pre-polymer and gel. Polym Int. 62. 534 – 547.
Loh, X., et.al. (2015). Poly (glicerol sebacate) biomaterial: synthesis and biomedical applications. Journal of Materials Chemistry. 3. 7641 – 7652. May et.al. (2011). Chapter 49 Regenerative Medicine in the Cornea. Principles of Regenerative Medicine. Elsevier. 911- 924.
Ortega, Iet.al. (2012). Combined microfabrication and electrospinning to produce 3-D architectures for corneal repair. Acta biomaterialia. 9. 5511 – 5520.
AcknowledgementsDr. Frederik Claeyssens
Dr. Ilida Asencio
Prof. Sheila MacNeiland
The University of Sheffield
National Science and Technology Council in Mexico (CONACyT) scholarship
Armourers and Brasiers companys grant
Learning societys funding
Characterization• Chemical
• FTIR• GPC (Mw 23,000 g/mol)
Scaffold design
Scaffold synthesis
Visual impairment of
the cornea is the 4th
global cause of
blindness
7 million of people go
blind every year
180 million of people
are blind or visually
disabled worldwide
Corneal
vascularization cause
blindness in 8 million
people (10% of total
blindness) worldwide
each year
Causes of LSCD:
•Hereditary:
Aniridia
Stevens Johnson´ s syndrome
• Acquired:
Thermal/ chemical burns
UV radiation
Poly (glycerol sebacate) methacrylate
Poly (glycerol sebacate)
Developed a safe and efficient corneal cell carrier.
This carrier should mimic the anatomical structure of the limbus to provide physical protection to the cells and
ensure their survival
4
.
PGS-M can be structured into complex designs models that mimic the curvature of the native cornea.
PGS-M is a biocompatible material suitable for corneal regeneration
Take home message
• The cornea has limbal stem cells (LSCs) that are in niches in the limbus
• LSCs are responsible for the corneal integrity and healthy balance
• LSCs are a proliferative barrier between the corneal epithelia and the conjunctiva and its blood vessels
• Limbal stem cells deficiency (LSCD) causes that this barrier breaks down, allowing conjunctival cells to move onto the cornea causing vascularization.
• LSCD produce scar tissue, causing pain, blushing and blindness
