LXS RI PANEL:LARGEST EXTANT RI SERIES

78 STRAINS

BETH BENNETTT.E. JOHNSON

UNIV OF COLORADOBOULDER, CO

DERIVATION OF ILS & ISS

8 WAY CROSS: A, AKR, BALB, C3H, C57, DBA, ISBI, RIII

HETEROGENOUS STOCK (HS)

LS & SS

ILS & ISS

Select for differential ethanolsensitivity for 25 generations

Inbreed 20 generations

RI CONSTRUCTION

BODY WT IN FEMALES

-Female weight chosen as mapping character-Not selected in progenitor strains-Possible interaction with fertility, lifespan-Easily measured test phenotype for LXS RI

-Measured at 30 day intervals in generation 13-94% inbred, 89% loci fixed

-Heritability estimated-QTLs mapped using MapManagerQT

GenotypingR.Williams and L. Lu

-Begun at N18

-All Parents within Strain Genotyped-Expect 96% of Loci Fixed-361 Polymorphic Markers Identified-Approximate Marker Density of 4.5 cM

30 DAY WEIGHTS

WT DISTRIBUTION AT 60, 90, 120, 150 DAYS

Heritability (h2), Genetic correlation (rG), and Phenotypic correlation (rP)

  30 Day 60 Day 90 Day 120 Day 150 Day

30 Day .49 .64 .53 .57 .56

60 Day .57 .62 .79 .83 .80

90 Day .49 .88 .66 .86 .91

120 Day .53 .83 .90 .64 .95

150 Day .53 .84 .89 .92 .72

FEMALE BODY WEIGHT IN LXS RIs AT 30 DAY INTERVALS

0

5

10

15

20

25

30

35

9 11 13 15 17 19 21 23 25 27

306090120150

WT IN GRAMS

GROWTH RATE AT 30 DAY INTERVALS

3.75

3.50

3.25

3.00

2.75

2.50

2.25

2.00

1.75

1.50

1.25

1.00

.75

.50

.25

0.00

-.25

-.50

-.75

-1.00

-1.25

A. 30-60 Days120

100

80

60

40

20

03.75

3.50

3.25

3.00

2.75

2.50

2.25

2.00

1.75

1.50

1.25

1.00

.75

.50

.25

0.00

-.25

-.50

-.75

-1.00

-1.25

B. 60-90 Days200

180

160

140

120

100

80

60

40

20

0

Gms Gained per Week

3.75

3.50

3.25

3.00

2.75

2.50

2.25

2.00

1.75

1.50

1.25

1.00

.75

.50

.25

0.00

-.25

-.50

-.75

-1.00

-1.25

C. 90-120 Days

N o

f M

ice

120

100

80

60

40

20

0

Gms Gained per Week

3.75

3.50

3.25

3.00

2.75

2.50

2.25

2.00

1.75

1.50

1.25

1.00

.75

.50

.25

0.00

-.25

-.50

-.75

-1.00

-1.25

D. 120-150 Days

N o

f M

ice

100

80

60

40

20

0

h2 for Growth Rate

 

Interval (Days)

Mean Growth Rate + SE

N strains

h2 (95% CI)

       

30-60 1.20 + 0.004 97 .48 (.39-.56)

60-90 0.49 + 0.003 86 .42 (.32-.50)

90-120 0.37 + 0.003 75 .25 (.15-.35)

120-150 0.25 + 0.002 63 .22 (.09-.33)

QTL MAPPING FOR BODY WT AT 30 DAYS

-2-101234567

D2Mit3

59

D2Mit8

1

D2Mit3

67

D2Mit9

0

D2Mit9

4

D2Mit4

03

D2Mit2

55

D2Mit2

57

D2Mit1

45

Chromosome 2

LRSEffect

QTL MAPPING FOR BODY WT AT 60 DAYS

-202468

1012141618

D11

Mit

2

D11

Mit

151

D11

Mit

173

D11

Mit

318

D11

Mit

90

D11

Mit

179

D11

Mit

360

D11

Mit

333

D11

Mit

11

Chromosome 11

LRSEffect

LOD=3.4

QTL MAPPING FOR BODY WT AT 90, 120 & 150 DAYS

-202468

10121416

D5M

it34

6

D5M

it34

4

D5M

it29

4

D5M

it25

1

D5M

it38

7

D5M

it26

7

D5M

it55

D5M

it30

9

D5M

it40

3

D5M

it27

8

D5M

it13

9

D5M

it37

2

D5M

it37

2

Chromosome 5

LRS90Effect90LRS120Effect120LRS150Effect150

LOD=3.2

PHENOTYPES BEING ASSESSED IN LXS RI

-ETHANOL STUDY:-Ethanol sensitivity & blood ethanol at awakening-Ethanol tolerance-Low dose ethanol activationAGING STUDY:-Lifespan-Dietary restriction responses

-Torpor, Food processing efficiency, Hair growth rate