DEPARTMENT OF HEALTH, ANIMAL SCIENCE AND ......Reproductive and Developmental Biology Laboratory Via...

Reproductive and Developmental Biology Laboratory Via Celoria, 10 - 20133 Milano, Italy Tel +39 02 50317969 - Fax +39 02 50317980 - email: [email protected] - www.redbiolab.unimi.it

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DEPARTMENT OF HEALTH, ANIMAL SCIENCE AND FOOD SAFETY

Alberto Maria Luciano, PhD Associate Professor

Biosketch - Eng 2017-01

Personal informations Date and place of birth: October 18, 1961, Napoli Married, father of Gabriele and Camilla Carlotta Current position and address: Associate Professor Reproductive and Developmental Biology Laboratory Department of Health, Animal Science and Food Safety University of Milan Via Celoria, 10 - 20133 Milano, Italy Studio (+39) 02 503 17969 Labs: (+39) 02 503 17978 -17982 - 17984 Fax: (+39) 02 503 17980 e-mail: [email protected] Team Leader of the Reproductive & Developmental Biology Laboratory: www.redbiolab.unimi.it Education 1991: Doctor in Biological Sciences, University of Milan, Italy 1993: PostDoctorate in Reproductive Physiology, University of Connecticut, School of Medicine, CT, USA. 1993: Professional habilitation (Biologist) 1998: PhD in Biotechnology applied to Veterinary Sciences, Faculty of Veterinary Medicine, University of Milan, Italy Professional activities 1987-1991 Trainee Fellowship for the “Association for the Advancement and Research in Nephrology and Dialysis”, in the Laboratory of Nephrology and Immunology, Division of Nephrology and Dialysis, S. Carlo Borromeo Hospital, Milan. 1992-1993 PostDoctorate Fellowship in Reproductive Physiology, University of Connecticut, School of Medicine, USA 1994-1995 Researcher for "Istituto Sperimentale Italiano Lazzaro Spallanzani", Milan, Italy 1998-2001 Research Assistant, Institute of Anatomy of Domestic Animals, Histology and Embryology* 1995-1998 PhD fellowship in Biotechnology applied to Veterinary Sciences*


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2001-2006 Researcher, Institute of Anatomy of Domestic Animals, Histology and Embryology* Since 2006 Associate Professor, Department of Animal Sciences, Section of Veterinary Anatomy and Histology* * Faculty of Veterinary Medicine, University of Milan. Courses, Faculty of Veterinary Medicine of Milan 2000 to 2005 Biology course, degree in “Animal Husbandry and Products Quality”. Since 2003 Teaching activity for the Doctorate School of “Sanità e Produzioni

animali: Scienze, tecnologie e biotecnologie” (Animal Production and Health: Science, technology and biotechnology)

Since 2006 Histology and Veterinary Embryology course, degree in Veterinary Medicine.

Since 2009 Anatomy of Domestic Animals course, degree in “Animal Husbandry and Welfare" (ABA)

Since 2014 Cell and tissue biology course, degree in "Animal Sciences" (SPA) Research fellowship and stages in other Institutions 1994 (September-November): Laboratorio di Tecnologie della Riproduzione - CIZ,

Cremona, Italy 1996(July): European Summer School in Embryotechnology, Royal Veterinary and

Agricultural University, Copenhagen (Denmark) 1997 (October-November): Universitè Laval, Departement des Sciences Animales,

Facultè des Sciences de l’Agriculture et de l’Alimentation, Ste-Foy, Quebec (Canada) (Europe-Canada Consortium Grant)

2001 (April-September): Unité de Physiologie de la Reproduction et des Comportements - Reproduction Equine, Institut National de la Recherche Agronomique (INRA) - Nouzilly (France) (OECD Grant Fellowship)

2002 (April): Deparment of Animal Reproduction and Artificial Insemination - National Research Center, Giza, (Egypt) (Nato-CNR Grant)

2002 (June-July): Unité de Physiologie de la Reproduction et des Comportements - Reproduction Equine, Institut National de la Recherche Agronomique (INRA) - Nouzilly (France) (INRA Grant)

2003 (June-August): Unité de Physiologie de la Reproduction et des Comportements - Reproduction Equine, Institut National de la Recherche Agronomique (INRA) - Nouzilly (France) (INRA/National Stud Grant)

2010 (April-May): University of Connecticut Health Center, Department of Cell Biology, USA (J1 Visiting Scientist)

2010 (October): Universitè Laval, Departement des Sciences Animales, Facultè des Sciences de l’Agriculture et de l’Alimentation, Ste-Foy, Quebec (Canada) (Visiting Scientist)


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2011 (December 2010-March 2011): University of Connecticut Health Center, Department of Cell Biology, USA (OECD grant Fellowship)

2012 (August): University of Kansas Health Center, Institute for Reproductive Health and Regenerative Medicine, Kansas City KS, USA (Visiting Scientist)

2014 (August): Universidade Estadual Paulista (UNESP) Campus de Botucatu - Instituto de Biociências (IBB), Sao Paulo, Brazil (Visiting Professor).

2014 (August): Empresa Brasileira de Pesquisa Agropecuária- Embrapa, Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia Brasilia, DF - Brasil (Visiting Professor).

2016 (October): Universitè Laval, Departement des Sciences Animales, Facultè des Sciences de l’Agriculture et de l’Alimentation, Ste-Foy, Quebec (Canada) (Visiting Professor)

2017 (February): Universidade Estadual Paulista (UNESP) Campus de Botucatu - Instituto de Biociências (IBB), Sao Paulo, Brazil (Visiting Professor).

Awards 2001 Co-Operative Research Programme Fellowships: Biological Resource

Management For Sustainable Agricultural Systems, awarded for: "Implication of oxidative stress during equine oocyte maturation, fertilization and early development" - Unité de Physiologie de la Reproduction et des Comportements - Reproduction Equine, Institut National de la Recherche Agronomique (INRA) - Nouzilly (France)

2010 Co-Operative Research Programme Fellowships: Biological Resource

Management For Sustainable Agricultural Systems, awarded for "Role of progesterone receptor membrane component-1 as a regulator of bovine oocyte viability and developmental competence" - Department of Cell Biology, University of Connecticul Health Center, CT, USA http://www.oecd.org/tad/crp/FELLOWSHIPSAWARDEDFOR2010WITHREPORTS_Website.pdf)

Associations Society for the Study of Reproduction (USA) since 1995. Society for Reproduction and Fertility (UK) since 1994. Endocrine Society (USA) 1996-2006. International Embryo Transfer Society (USA) 1998-2008. European Society for Domestic Animal Reproduction (UE) since 1999-2006. Associazione Italiana Morfologi Veterinari, (IT), 2003-2011. European Society of Human Reproduction and Embryology, since 2014 Offices held


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2002-2016: Member of Veterinary Medicine Library Scientific Board Since 2016: Scientific director of Veterinary Medicine Library of UNIMI 2007-2012: Coordinator of the Division of Veterinary Anatomy and Histology, Department of Veterinary Sciences, University of Milan 2008-2012 Vice Director of the Department of Animal Sciences 2008-2014 Member of the board of "Interdepartmental Centre for Studies on Mammary Gland" (CISMA) University of Milan Since 2011 Member of the board and Founder and from 2014 to 2016 Director of the"Interdepartmental Research Centre for the Study of Biological Effects of Nano-concentrations" (CREBION) University of Milan Since 2006 Member of the Board of the Doctorate School in Animal Production and Health: Science, technology and biotechnology and since 2013 member of the Directive Board of the Doctorate course in Veterinary Animal Sciences, University of Milan. Reviewer for Scientific projects for: Horizon 2020 Natural Sciences and Engineering Research Council of Canada (NSERC) Government of Canada University of Ghent (B) Irish Research Council MIUR Young investigator MIUR VQR Università dell’Insubria Università degli Studi di Sassari Università degli Studi di Teramo Banco di Sardegna Board of Referees Official reviewer for the following indexed Journals with IF: Aging and Disease Anatomical Record Anatomia Histologia Embryologia Animal Biotechnology


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Animal Reproduction Animal Reproduction Science Animal Science Journal Asian Pacific Journal of Reproduction (Editorial Board) BioFactors Biological Sciences BioMed Research International Biotechnology Letters BMC Genomics Cell Physiology and Biochemistry Cell Research Current Genomics Biology of Reproduction European Journal of Histochemistry European Journal of Obstetrics, Gynecology and Reproductive Biology FASEB Journal Frontiers in Neuroendocrine Science Human Reproduction International Journal of Developmental Biology International Journal of Fertility and Sterility International Journal of Radiation Biology Journal of Assisted Reproduction and Genetics Journal of Animal Science and Biotechnology (Editorial Board) Journal of Dairy, Veterinary & Animal Research (Editorial Board) Journal of Genetics Journal of Histochemistry & Cytochemistry Journal of Human Reproductive Sciences Journal of Ovarian Research Journal of Visualized Experiments Livestock Science Microscopy Research and Technique Molecular Human Reproduction Molecular Reproduction and Development Physiological Genomics PLoS ONE Reproduction Reproduction, Fertility and Development Reproduction in Domestic Animals Reproductive Biology and Endocrinology Reproductive Sciences Scientific Report Systems Biology in Reproductive Medicine Theriogenology Toxicology Reports Zygote


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Research interests Reproductive and developmental biology. Specific topics: Regulation of chromatin remodeling during oocyte differentiation Progesterone and mammalian infertility Epigenetic changes in assisted reproductive technologies Reproductive toxicology Active Collaborations Department of Cell Biology, University of Connecticut Health Center, Farmington, CT (USA) - Prof. John J. Peluso. Departement des Sciences Animales University of Laval, Ste-Foy, and Embryogene Network, Quebec, (Canada) - Prof. Marc-Andre Sirard and Prof. Claude Robert Unite de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique (INRA) Centre de Tours, Nouzilly (France) (MoU UniMI for International Cooperation 2013-2017) - Dr. Ghylene Goudet and Dr. Nadine Gerard. EUGIN Fertility Clinic, Barcelona, Spain (EUGIN-EUVITRO International Cooperation Agreement 2015-2020) - Dr. Rita Vassena AVANTEA - Laboratorio di Tecnologie della Riproduzione (LTR), Cremona (Italy) Prof. Cesare Galli, Dr. Giovanna Lazzari Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, Royal Veterinary and Agricultural University, Copenhagen (Denmark) - Prof. Poul Hyttel Department of Morphology, Biochemistry, Physiology and Animal Production, Faculty of Veterinary Medicine, University of Messina, Messina, (Italy) - Prof. Franco Abbate, Prof. Germana P. Germanà Dipartimento di Biologia Animale, Dipartimento di Scienze Fisiologiche, Biochimiche e Cellulari, Universita degli Studi di Sassari, Facolta di Medicina Veterinaria, Sassari (Italy) - Prof. Salvatore Naitana, Dr. Giovanni G. Leoni Department of Physiology, Institute of Biosciences, Sao Paulo State University (UNESP), Rubiao Junior, Botucatu, Sao Paulo, Brazil (International Cooperation Agreement 2015-2020) - Prof. José Buratini Jr.


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University of New South Wales, Discipline of Obstetrics & Gynaecology, School of Women’s & Children’s Health, Sydney, Australia - Prof. Robert Gilchrist The Center for Human Reproduction, NY (USA) - Prof. David Albertini


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Published articles More than 200 publications, over 70 peers reviewed articles indexed in the main databases (PubMed, WOK, Scopus, CAB). Main Ongoing and completed Research Grant (last 6 years)

FP7-PEOPLE-2013-IOF Marie Curie Actions - International Outgoing Fellowships (IOF) Contract n. 624874 (March 2014 - February 2017) Principal Investigator: Federica Franciosi Scientific Coordinator: Alberto M. Luciano Outgoing phase: Marco Conti, University of California, San Francisco MateRNA - Somatic cells regulation of maternal mRNA translation in mammalian oocytes Regione Autonoma della Sardegna e Regione Lombardia, Accordo di collaborazione alla cooperazione scientifica e tecnologica nelle aree tematiche biotecnologiche e ICT, Prog. "Ex Ovo Omnia", Grant n. 26096200 (Dec 2011- Dec 2015) Role: Principal investigator Project: Sviluppo di sistemi fisiologici per la maturazione in vitro dell’ovocita nei mammiferi domestici ai fini del miglioramento dell’efficienza delle biotecnologie di riproduzione assistita. Aim: Research activities in the development of pre-maturation and in vitro maturation systems of pig and horse oocytes and effects on the process of chromatin remodeling. BIOGENESI Centro di Medicina Riproduttiva, Research contract (Dec 2011-Aug 2013) Role: Principal investigator Project: Development of a physiological culture system to improve mammalian oocyte in vitro developmental competence. Aim: Research activities in the development of pre-maturation culture system of bovine oocytes and effects on the process of chromatin remodeling and developmental competence. EmbryoGENE Network (Jun 2011 - Jun 2014) Departement des Sciences Animales, Université Laval, Quebec, CANADA Role: Scientific Coordinator Project: Development of a transcriptomic platform to identify markers that can predict mammalian oocyte competence Aim: Proposal drafting and research activities focused on studies on large scale chromatin remodelling and transcripomics in bovine oocyte. FP7-PEOPLE-2011-CIG, Research Executive Agency (REA) Contract n. 303640 (Jan 2012- Jan 2016)


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Principal investigator: Valentina LODDE Role: Scientific Coordinator Project: Role of Progesterone Receptor Membrane Component-1 in oogenesis and mammalian fertility (PRO-OVUM) Aim: Role of PGRMC1 in infertility and molecular mechanisms involved in oocyte developmental competence acquisition. CREBION (Research Contract Grant: Guna Spa) (Sept 2012 - Dec 2013) Role: Scientific coordinator Project: Study of low-dose hormone therapy in the policystic ovarian syndrome (PCOS) in the mouse Aim: Research activities focused on the assessment of the effect of omeopatic products on Polycistic Ovarian Syndrome (PCOS) induced in mouse as animal model. CONSORZIO MILANO RICERCHE (Research Contract Grant: Guna spa) (Apr 2011 - Dec 2011) Role: Scientific Coordinator Project: Study of low-dose hormone therapy in the policystic ovarian syndrome (PCOS) in the mouse Aim: Research activities focused on the assessment of the effect of omeopatic products on Polycistic Ovarian Syndrome (PCOS) induced in mouse as animal model. Fellowship Awards Programme 2010, Contract: JA00064361 (Dec 2010 - Mar 2011) OECD Co-operative Research Programme: Biological Resource Management for Sustainable Agricultural System Role: Principal investigator Project title: Role of Progesterone Receptor Membrane Component-1 as a Regulator of Bovine Oocyte Viability and Developmental Competence Aim: Interaction PCRMC1 and AURKB during mammalian oocyte meiosis. Ateneum Research Grant PUR 2009-ATE-0410 University of Milan (Jan 2010 - Dec 2010) Role: Co-Investigator Project: Characterization of microRNAs involved in the apoptotic process of bovine monocites. Aim: Identification of miRNAs involved in the regulation of the processes of apoptosis of bovine monocytes, assessed by caspase 7 activity. EU FP6 "Consortium REPROTECT" (Jun 2008 - Jan 2010) Role: Principal investigator, Team leader Member n. 38. Contract n° LSHB-CT-2004-503257 (http://www.reprotect.eu/) Project: Development of a novel approach in hazard and risk assessment of reproductive toxicity by a combination and application of in vitro, tissue and sensor technologies. ReProTect is an Integrated Project of the EU (IP) funded within the 6th Framework Programme of the EC (Coordinator: Prof. Michael Schwarz, (EKUT) University of


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Tuebingen, Institute of Pharmacology and Toxicology, Department of Toxicology, Tuebingen, Germany). Aim: To explore the most complicate and delicate field of toxicology, the reproductive toxicity. The main objective of this grant is to validate the bovine in vitro maturation, fertilization and embryonic development test for risk assessment of reproductive toxicity. Ateneum research grant PUR 2008-5284002-27, University of Milan (Jan 2009 - Dec 2009) Role: Principal investigator Project: Role of Progesterone Receptor Membrane Component-1 (PGRMC-1) in oocyte vitality and competence. Aim: Proposal drafting and research activities focused on the localization and involvement of PGRMC1 in meiotic division and its colocalization with chromosome passenger complex protein(s). Ateneum research grant PUR 2007 University of Milan (Jan 2008-Dic 2008) Role: Principal investigator Project: Nuclear Transplantation in the bovine as a model of ooplasmic transplantation for the treatment of ageing related errors in the oocyte. Aim: Research activities focused on the assessment of mitochondrial activity and localization after ooplasm transplantation. PRIN 2005 - PROT. 2005073071-003 (20/01/2006 - 08/03/2008) Role: Principal investigator Project: Nuclear and cytoplasmic events accompanying bovine oocyte developmental competence acquisition. Aim: To identify parameters to study molecular events involved in oocyte developmental capacity acquisition, starting from the characterization of chromatin remodeling process, in order to explore the event and the mechanisms involved in the female gamete differentiation. Projects applied and scored


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Recent and relevant peer-reviewed pubblications (last 6 years)

1. Lodde V, Luciano AM, Franciosi F, Labrecque R, Sirard MA. Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes. Results Probl Cell Differ 2017; 63:223-255.

2. Soares ACS, Lodde V, Barros RG, Price CA, Luciano AM, Buratini J (2017)

Steroid hormones interact with natriuretic peptide type C to delay nuclear maturation, to maintain oocyte-cumulus communication and to improve quality of in vitro produced embryos in cattle. Reproduction, fertility, and development RD16320 2017; doi:10.1071/RD16320.

3. Tessaro I, Modina SC, Lodde V, Sivelli G, Franciosi F, Terzaghi L, Luchini P,

Rumio C, Luciano AM. Ultra-low Doses of Follicle Stimulating Hormone and Progesterone Attenuate the Severity of Polycystic Ovary Syndrome Features in a Hyperandrogenized Mouse Model. J Reprod Infertil 2017; 18:288-298.

4. Franciosi F, Tessaro I, Dalbies-Tran R, Douet C, Reigner F, Deleuze S, Papillier

P, Miclea I, Lodde V, Luciano AM, Goudet G. Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes. J Vis Exp 2017:(123)1-8.

5. Terzaghi L, Tessaro I, Raucci F, Merico V, Mazzini G, Garagna S, Zuccotti M,

Franciosi F, Lodde V (2016) PGRMC1 participates in late events of bovine granulosa cells mitosis and oocyte meiosis. Cell Cycle 15 (15):2019-2032. doi:10.1080/15384101.2016.1192731.

6. Dieci C, Lodde V, Labreque R, Dufort I, Tessaro I, Sirard MA, Luciano AM.

Differences in cumulus cell gene expression indicate the benefit of a pre-maturation step to improve in-vitro bovine embryo production. Mol Hum Reprod 2016; 10.1093/molehr/gaw055.

7. Luciano AM, Peluso JJ. PGRMC1 and the faithful progression through mitosis

and meiosis. Cell Cycle 2016; 15:2239-2240.

8. Gilchrist RB, Luciano AM, Richani D, Zeng H, Wang X, De Vos M, Sugimura S, Smitz J, Richard FJ, Thompson JG. Oocyte maturation and quality: role of cyclic nucleotides. Reproduction 2016;152: R143-157.

9. Tessaro I, Modina SC, Franciosi F, Sivelli G, Terzaghi L, Lodde V and Luciano

AM. Effect of oral administration of low-dose follicle stimulating hormone on hyperandrogenized mice as a model of polycystic ovary syndrome. J Ovarian Res 2015; 8:64.


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10. Franciosi F, Goudet G, Tessaro I, Papillier P, Dalbies-Tran R, Reigner F, Deleuze S, Douet C, Miclea I, Lodde V and Luciano AM. Oocyte in vitro maturation affects chromosome segregation, spindle morphology and H4K16 acetylation in the horse. Reprod Fertil Dev 2015; (doi: 10.1071/RD15350).

11. Labrecque R, Lodde V, Dieci C, Tessaro I and Luciano AM and Sirard MA.

Chromatin remodeling and histone mRNA accumulation in bovine germinal vesicle oocytes. Mol Reprod Dev 2015; 10.1002/mrd.22494.

12. Tessaro I, Modina SC, Crotti G, Franciosi F, Colleoni S, Lodde V, Galli C, Lazzari

G and Luciano AM. Transferability and inter-laboratory variability assessment of the in vitro bovine oocyte fertilization test. Reprod Toxicol 2015; 25;51C:106-113. 10.1016/j.reprotox.2015.01.001.

13. Franciosi F, Coticchio G, Lodde V, Tessaro I, Modina SC, Fadini R, Dal Canto M,

Mignini Renzini M, Albertini DF and Luciano AM. Natriuretic Peptide Precursor C Delays Meiotic Resumption and Sustains Gap Junction Mediated Communication in Bovine Cumulus Enclosed Oocytes. Biol Reprod 2014; 91:61. doi.10.1095/biolreprod.1114.118869.

14. Scantland S, Tessaro I, Macabelli CH, Macaulay AD, Cagnone G, Fournier E,

Luciano AM and Robert C. The Adenosine Salvage Pathway as an Alternative to Mitochondrial Production of ATP in Maturing Mammalian Oocytes. Biol Reprod 2014:10.1095/biolreprod.1114.120931.

15. Luciano AM, Franciosi F, Dieci C, Tessaro I, Terzaghi L, Modina SC and Lodde

V. Large-scale chromatin structure and function changes during oogenesis: the interplay between oocyte and companion cumulus cells. Anim Reprod 2014; 11:141-149.

16. Modina SC, Tessaro I, Lodde V, Franciosi F, Corbani D and Luciano AM Luciano

AM. Reductions in the number of mid-sized antral follicles is associated with premature ovarian senescence in dairy cows. Reprod Fertil Dev 2014; 26(2): 235-244.

17. Galli C, Colleoni S, Turini P, Crotti E, Dieci C, Lodde V, Luciano AM and

Lazzari G. Holding equine oocytes at room temperature for 18 hours prior to in vitro maturation maintains their developmental competence. J Equine Vet Sci 2014; 34:174-175.

18. Luciano AM, Franciosi F, Dieci C, Lodde V. Changes in large-scale chromatin

structure and function during oogenesis: A journey in company with follicular cells. Anim Reprod Sci 2014:149, 3-10. doi.1016/j.anireprosci.2014.06.026.

19. Dieci C*, Lodde V*, Franciosi F, Lagutina I, Tessaro I, Modina SC, Albertini DF,

Lazzari G, Galli C and Luciano AM. The effect of cilostamide on gap junction


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communication dynamics, chromatin remodeling, and competence acquisition in pig oocytes following parthenogenetic activation and nuclear transfer. Biol Reprod 2013; 89(3):68, 1-11 (*Contributed equally).

20. Lodde V, Franciosi F, Tessaro I, Modina SC and Luciano AM. Role of gap

junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte. J Assist Reprod Genet 2013. doi:10.1007/s10815-013-0061-7.

21. Luciano AM, Franciosi F, Lodde V, Tessaro I, Corbani D, Modina SC and Peluso

JJ. Oocytes Isolated from Dairy Cows with Reduced Ovarian Reserve Have a High Frequency of Aneuploidy and Alterations in the Localization of Progesterone Receptor Membrane Component 1 and Aurora Kinase B. Biol Reprod 2013; 88(3):58, 1-7.

22. Luciano AM and Lodde V. Changes of Large-Scale Chromatin Configuration

During Mammalian Oocyte Differentiation. In: Coticchio G, Albertini DF, De Santis L (eds.), Oogenesis. London: Springer-Verlag London; 2013: 93-108.

23. Luciano AM, Lodde V, Franciosi F, Tessaro I, Corbani D, Modina S. Large-scale

chromatin morpho-functional changes during mammalian oocyte growth and differentiation. Eur J Histochem 2012; 56(3):e37. doi: 10.4081/ejh.2012.e37

24. Franciosi F, Lodde V, Goudet G, Duchamp G, Deleuze S, Douet C, Tessaro I and

Luciano AM. Changes in histone H4 acetylation during in vivo versus in vitro maturation of equine oocytes. Mol Hum Reprod 2012;18(5):243-52.

25. Luciano AM, Franciosi F, Modina SC, Lodde V. Gap Junction-Mediated

Communications Regulate Chromatin Remodeling During Bovine Oocyte Growth and Differentiation Through cAMP-Dependent Mechanism(s). Biol Reprod 2011; 85:1252-1259.

26. Luvoni GC, Tessaro I, Apparicio M, Ruggeri E, Luciano AM, Modina S. Effect of

vitrification of feline ovarian cortex on follicular and oocyte quality and competence. Reprod Domest Anim 2012; 47(3):385-91.

27. Luciano AM, Corbani D, Lodde V, Tessaro I, Franciosi F, Peluso JJ, Modina S.

Expression of Progesterone Receptor Membrane Component-1 in bovine reproductive system during estrous cycle. Eur J Histochem 2011; doi: 10.4081/ejh.2011; 55(3):e27.

28. Tessaro I, Luciano AM, Franciosi F, Lodde V, Corbani D, Modina SC. The

endothelial nitric oxide synthase/nitric oxide system is involved in the defective quality of bovine oocytes from low mid-antral follicle count ovaries. J Anim Sci 2011; 89:2389-2396.


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29. Sassera D, Lo N, Epis S, D'Auria G, Montagna M, Comandatore F, Horner D,

Pereto J, Luciano AM, Franciosi F, Ferri E, Crotti E, et al. Phylogenomic Evidence for the Presence of a Flagellum and cbb3 Oxidase in the Free-Living Mitochondrial Ancestor. Mol Biol Evol 2011; 28(12):3285-96.

30. Lorenzetti S, Altieri I, Arabi S, Balduzzi D, Bechi N, Cordelli E, Galli C, Ietta F,

Modina SC, Narciso L, Pacchierotti F, Villani P, Lazzari G, Luciano AM, Paulesu L, Spano M and Mantovani A. Innovative non-animal testing strategies for reproductive toxicology: the contribution of Italian partners within the EU project ReProTect. Ann Ist Super Sanita 2011; 47:4 429-444.

31. Modina S, Leoni GG, Lodde V, Naitana S, Pirani S, Succu S, Berlinguer F and

Luciano AM. Involvement of E-cadherin in early in vitro development of adult and juvenile sheep embryos. Reprod Fertil Dev 2010; 22:468-477.

32. Luciano AM, Lodde V, Franciosi F, Ceciliani F and Peluso JJ. Progesterone

receptor membrane component-1 expression and putative function in bovine oocyte maturation, fertilization and early embryonic development. Reproduction 2010; 140(5):663-72.

33. Luciano AM, Franciosi F, Lodde V, Corbani D, Lazzari G, Crotti G, Galli C,

Pellizzer C, Bremer S, Weimer M and Modina SC. Transferability and inter-laboratory variability assessment of the in vitro bovine oocyte maturation (IVM) test within ReProTect. Reprod Toxicol 2010; 30(1):81-8.

34. Franciosi F, Perazzoli F, Lodde V, Modina SC and Luciano AM. Developmental

competence of gametes reconstructed by germinal vesicle transplantation from fresh and cryopreserved bovine oocytes. Fertil Steril 2010; 93:229-38.

35. Epis S, Luciano AM, Franciosi F, Bazzocchi C, Crotti E, Pistone D, Bandi C,

Sassera D. A novel method for the isolation of DNA from intracellular bacteria, suitable for genomic studies. Annals of Microbiology 2010; 60:455-60.

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