DEPARTMENT OF BOTANY AND MICROBIOLOGY ... CBCS 2017-2018.pdfDEPARTMENT OF BOTANY AND MICROBIOLOGY...

DEPARTMENT OF BOTANY AND MICROBIOLOGY

Syllabus for Microbiology Approved in B.O.S. for the Academic Year 2017-2018

Dated 13-04-2017

PPPPITHAPUR RAJAHSITHAPUR RAJAHSITHAPUR RAJAHSITHAPUR RAJAHS GOVTGOVTGOVTGOVT.... COLLEGECOLLEGECOLLEGECOLLEGE

(Autonomous and NAA(Autonomous and NAA(Autonomous and NAA(Autonomous and NAAC ReC ReC ReC Re----accredited with Baccredited with Baccredited with Baccredited with B----2.78 CGPA)2.78 CGPA)2.78 CGPA)2.78 CGPA)

KAKINADA – 533 001, A.P., INDIA

PITHAPUR RAJAHS GOVERNMENT COLLEGE (AUTONOMOUS), KAKINADA, E.G.DT., ANDHRA PRADESH

DEPARTMENT OF BOTANY AND MICROBIOLOGYDEPARTMENT OF BOTANY AND MICROBIOLOGYDEPARTMENT OF BOTANY AND MICROBIOLOGYDEPARTMENT OF BOTANY AND MICROBIOLOGY The Board of Studies meeting for MICROBIOLOGYMICROBIOLOGYMICROBIOLOGYMICROBIOLOGY subject for the academic year 2017-18 is conducted at the Dept. of Botany and Microbiology on 13-04-2017 at 10;00 AM with Dr. A. Srinivasa Rao, Lecturer in-Charge in the chair along with the following members. ------------------------------------------------------------------------------------------------------------ ------------------- Name, designation and address Signature

------------------------------------------------------------------------------------------------------------------------------- 1. Chairperson :

Dr. A. Srinivasa Rao, Lecturer – In-Charge Dept. of Botany and Microbiology P.R.G.C.(A), Kakinada 2. A.K.N.University, Rajahmundry Nominee :

Dr. D.Kalyani, Assistant Professor Dept. of Zoologt, Adikavi Nannaya University Rajamahendravaram, East Godavari District, A.P. 3. Members nominated by Executive council of the College :

a. Member from Research Domain:

Dr. Ch.Usha Rani Principal Investigator DST/Disha (Woman Sicentist Project) Dept. of Botany and Microbiology P.R. Govt. College(A) Kakinada, E.G.Dt., A.P. b. Subject expert – Local nominee :

Dr. Smt. K. Aruna, Lecturer in Microbiology A.S.D. Govt. Degree College for Women (A),

Kakinada, E.G.Dt., A.P. c. Member from Industry :

Smt. P. Swathi, Assistant Director Biological Control Laboratory

Dept. of Agriculture, Near RTO office, Kakinada

4. Members from the College : a. Faculty members : Ms. Y. Anita, Guest faculty in Microbiology b. Student members :

C.B.C.S. pattern effective from 2014-2015 Academic Year Microbiology Model Blue Print for the Question paper and choice for I year (w.e.f.2017-18 Academic Year)

S.No.

Type of Questions

To be given in the Question paper

To be answered

No. of

Questions

Mark

s

allotted to

each

question

Total mark

s

No. of

Questions

Mark

s

allotted to

each

question

Total mark

s

1

SECTION - A Very Short Answer Questions

05 01 05 05 01 05

2 SECTION - B Short Answer Questions

08 03 24 05 03 15

3 SECTION - C Essay Questions (Either- or)

10 08 80 05 08 40

Total questions & Total marks =

23 - 109 15 - 60

109 - 60 49 Percentage of choice given = ------------ x 100 = ------ x 100 = 44.95%

109 109

I B.Sc., – Microbiology / I Semester End (W.E.F. 2017-2018)

Introduction to Microbiology and microbial diversity (Course : MB1209) Total Hrs. of Teaching-Learning: 60 @ 4 h / Week Total Credits : 03

Learning objectives : 1. To teach the historical events in Microbiology. 2. To provide knowledge on diversity of micro-organisms. 3. To instill students on laboratory techniques like microscopy, sterilization and culture of microbes. Learning out-comes : 1. Student will acquaint with history of Microbiology and diversity of microbes. 2. Leaner can carry out staining and sterilization techniques in a microbiology laboratory and can culture microbes.

UNIT-I History of Microbiology 10 Hrs.

a. History and mile stones in Microbiology. Contributions of Anton von Leeuwenhoek, Edward Jenner, Louis Pasteur, Robert Koch, Iwanowsky.

b. Importance and applications of Microbiology. c. Classification of microorganisms – Haeckel’s three Kingdom concept, Whittaker’s

five kingdom concept, three domain concept of Carl Woese. UNIT – II Bacteria and Viruses 14 Hrs.

a. Outline classification of bacteria as per the second edition of Bergey’s Manual of Systematic Bacteriology.

b. General characteristics of Bacteria, Archaea, Mycoplasmas and Cyanobacteria. c. Ultra structure of Prokaryotic cell- Variant components and invariant components. d. General characteristics of viruses. morphology, structure and replication of TMV and

HIV. UNIT-III Other microbes and microscopy 10 Hrs.

a. General characteristics and outline classification of Fungi, Algae and Protozoa. b. Principles of microscopy - Bright field and Electron microscopy (SEM and TEM). UNIT-IV Staining and sterilization techniques 14 Hrs.

a. Staining techniques –Simple and Differential (Gram Staining and Spore Staining). b. Sterilization and disinfection techniques - Physical methods – autoclave, hot- air

oven, pressure cooker, laminar air flow, filter sterilization, Radiation methods – UV rays, Gamma rays.

c. Chemical methods – alcohols, aldehydes, fumigants, phenols, halogens and hypochlorites.

UNIT –V Culture techniques 12 Hrs.

a. Isolation of Microorganisms from natural habitats. b. Pure culture techiques – dilution-plating, Streak-plate, Spread-plate, Pour-Plate and

micromanipulator. Enrichment culturing. c. Preservation of microbial cultures – sub-culturing, overlaying cultures with mineral

oils, lyophilization, sand cultures, storage at low temperature.

I B.Sc., – I Semester - Microbiology Pracical Syllabus (W.E.F. 2017-2018) Introduction to Microbiology and microbial diversity (Course : MB1209P)

Total Hrs. of Pracicals : 30 @ 2 h / Week Total Credits : 02

1. Microbiology Good Laboratory Practices and Biosafety.

2. Preparation of culture media for cultivation of bacteria

3. Preparation of culture media for cultivation of fungi

4. Sterilization of medium using Autoclave

5. Sterilization of glassware using Hot Air Oven

6. Light compound microscope and its handling

7. Microscopic observation of bacteria (Gram +ve bacilli and cocci, Gram -ve bacilli), Cyanobacteria, Algae and Fungi. 8. Simple staining

9. Gram’s staining

10. Hanging-drop method.

11. Isolation of pure cultures of bacteria by streaking method.

12. Preservation of bacterial cultures by various techniques.

13. Diagramatic or Electron photo-micrographic observation of TMV, HIV, T4 phage and Adenovirus. Suggested readings :

� Alexopoulos, C.J., Mims, C.W. and Blackwell, M. (1996). Introductory Mycology, Wiley, New York.

� Atlas, R.A. and Bartha, R. (2000). Microbial Ecology. Fundamentals and

Application, Benjamin Cummings, New York. � Dimmock, N.J., Easton, A.J. and Leppard, K.N. (2001). Introduction to

Modern Virology, Blackwell Science Ltd, U.K. � Dube, R.C. and Maheswari, D.K. (2000) General Microbiology. S Chand,

New Delhi.Edition), Himalaya Publishing House, Mumbai. � Frobisher, H., Hinsdil, R.D., Crabtree, K.T. and Goodhert, D.R. (2005).

Fundamentals of Microbiology, Saunder and Company, London. � Jaya Babu (2006). Practical Manual on Microbial Metabolisms and General

Microbiology. Kalyani Publishers, New Delhi. � Madigan, M.T., Martinkl, J.M. and Parker, J. (2010). Brock Biology of

Microorganisms, 9th Edition, MacMillan Press, England. � Pelczar, M.J., Chan, E.C.S. and Kreig, N.R. (1993). Microbiology. 5th Edition,

Tata Mc Graw Hill Publishing Co., Ltd., New Delhi. � Rao, A.S. (1997). Introduction to Microbiology. Prentice-Hall of India Pvt

Ltd., Nerw Delhi.

P.R.Government College (Autonomous), Kakinada I year B.Sc., Program I Semester End

Microbiology I A : Introduction to Microbiology and microbial diversity (Course Code MB1209 w.e.f. 2017-2018)

Time : 2½ hours Max. Marks:60

SECTION – A

Answer all the following questions 5 x 1 = 05 M 1. Iwanowsky 2. Mycoplsma 3. SEM 4. Spore staining 5. Streak plate

SECTION – B

Answer any five questions 5 x 3 = 15 M 6. Robert Koch 7. Gram staining 8. U.V. Rays 9. Spread plate method 10. Cell membrane 11. HIV 12. Spore staing 13. Lyophilization

SECTION – B Answer all the Questions. Draw labeled diagrams wherever necessary 5 x 8 = 40 M 14. a) Discuss the various contributions of Louis Pasteur and Edward Jenner?

(Or) b) Discuss the Five Kingdom concept?

15. a) Write in detail about the structure of prokaryotic cell (Or)

b) Discuss general characters of viruses?

16. a) Describe the principle and mechanism of Fluorescent microscopy? (Or)

b) General characters and classification of Algae?

17. a) Explain in detail about Physical methods of sterilization? (Or)

b) Explain different staining techniques?

18. a) Write in detail about isolation of microorganisms from natural habitat? (Or)

b) Write an essay on various methods of microbial preservation techniques?

I B.Sc., Microbiology - Practical Examinations Paper I model at the end of I Semester (w.e.f. 2017-18) Introduction to Microbiology and microbial diversity (MB1209P)

Time: 2 Hrs. Max. Marks: 50

1. Identify the given organism ‘A’ by Gram staining technique. 10 M

Scheme for valuation: Preparation of slide (05M) + Description (3M) + Result (2M)

2. Preparation of culture medium ‘B’. 10 M

Scheme for valuation: Principle (5M) + Procedure (5M)

3. Dilution and isolation technique ‘C’. 10 M Scheme for valuation: Practical work (5M) + Description (5M)

4. Identify and write notes on the following 5 x 2 = 10 M D. Virus/Protozoa E. Bacteria/Cyanobacteria F. Algae G. Fungi H. Apparatus Scheme for valuation: Identification (1M) + Reasons (2M)

5. Record + Viva voce 6 +4 = 10 M --------------------------------------------------------------------------------------------------------------------- Total = 50 M ---------------------------------------------------------------------------------------------------------------------

I B.Sc., – Microbiology / II Semester End (W.E.F. 2017-2018)

Introduction to Microbial Biochemistry and Metabolism (Course : MB2209) Total Hrs. of Teaching-Learning: 60 @ 4 h / Week Total Credits : 03

Learning objectives : 1. To give thorough knowledge on biomolecules and their characterization/quantification. 2. To endow with basics of Enzymology and nutrition and metabolism in microbes. Learning out-comes : 1. Student can characterize different biomolecules and and estimation them through instrumentation. 2. Leaner will be able to explain nutrition and metabolism in microbes.

UNIT-I Biomolecules 12 Hrs

a. Outline classification and general characteristics of carbohydrates (monosaccharides, disaccharides and polysaccharides).

b. General characteristics of amino acids and proteins. c. Structure of nitrogenous bases, nucleotides, nucleic acids. d. Fatty acids (saturated and unsaturated). Lipids (sphingolipds, sterols and

phospholipids). UNIT-II Instrumentation techniques 08 Hrs

a. Principle and applications of - Colorimerty b. Chromatography (paper, thin-layer and affinity chomatography ) c. Spectrophotometry (UV & visible). Centrifugation UNIT-III Basics of Enzymology 12 Hrs.

a. Properties and classification of Enzymes. Biocatalysis- induced fit and lock and key models.

b. Role of Coenzymes and Cofactors in enzyme activity. Factors affecting catalytic activity of enzymes.

c. Inhibition of enzyme activity- competitive, noncompetitive, uncompetitive and allosteric.

UNIT-IV Nutrition and growth of microbes 14 Hrs.

a. Microbial Nutrition –Nutritional requirements and uptake of nutrients by cells. Nutritional groups of microcroorganisms- autotrophs, heterotrophs, mixotrophs.

b. Outlines of oxygenic and anoxygenic photosynthesis in bacteria c. Growth media- synthetic, complex, selective, enrichment and differential media. d. Microbial Growth- different phases of growth in batch cultures, Synchronous,

continuous, biphasic growth. Factors influencing microbial growth. e. Methods for measuring microbial growth – Direct microscopy, viable count

estimates, turbiodometry and biomass. UNIT-V Metabolism 12 Hrs.

a. Aerobic respiration -Glycolysis, HMP path way, ED path way, TCA cycle, Electron transport, oxidative and substrate level phosphorylation. Anaerobic respiration (Nitrate).

b. Fermentation - Alcohol and lactic acid fermentations.

I B.Sc., – I Semester - Microbiology Pracical Syllabus (W.E.F. 2017-2018)

Introduction to Microbiology and microbial diversity (Course : MB2209P)

Total Hrs. of Pracicals : 30 @ 2 h / Week Total Credits : 02

1. Qualitative Analysis of Carbohydrates

2. Qualitative Analysis of Amino acids

3. Colorimetric estimation DNA by diphenylamine method

4. Colorimetric estimation of proteins by Biuret/Lowry method

5. Paper chromatographic separation of sugars / amino acids

6. Preparation of different media- Synthetic and Complex Media

7. Setting and observation of Winogradsky column.

8. Estimation of CFU count by spread plate method/pour plate method.

9. Bacterial growth curve.

10. Factors affecting bacterial growth – pH.

11. Factors affecting bacterial growth – Temperature.

12. Factors affecting bacterial growth –Salts

Suggested readings :

� Lehninger, A.L., Nelson, D.L. and Cox, M.M. (1993). Principles of Biochemistry, 2 nd Edition, CBS Publishers and Distributors, New Delhi.

� Prescott, M.J., Harley, J.P. and Klein, D.A. (2010). Microbiology. 5th Edition, WCB Mc GrawHill, NewYork.

� Reddy, S.R. and Reddy, S.M. (2004). Microbial Physiology, Scientific Publishers, Jodhpur, India.

� Sashidhara Rao, B. and Deshpande, V. (2007). Experimental Biochemistry: A student Companion. I.K.International Pvt. Ltd. India Pvt. Ltd., New Delhi.

� Tymoczko JL, Berg JM and Stryer L (2012) Biochemistry: A short course, 2nd ed., W.H.Freeman

� Voet,D. and Voet J.G (2004) Biochemistry 3rd edition, John Wiley and Sons � White, D. (1995). The Physiology and Biochemistry of Prokaryotes, Oxford

University Press, New York. � Willey MJ, Sherwood, LM & Woolverton C J (2013) Prescott, Harley and

Klein’s Microbiology by.9th Ed., McGrawHill

P.R.Government College (Autonomous), Kakinada I year B.Sc., Program II Semester End

Microbiology I B : Introduction to Microbial Biochemistry and Metabolism (Course Code MB 2209 w.e.f. 2014-2015)


SECTION – A Answer all the following questions 5 x 1 = 05 M 1. Sterols 2. Centrifugation 3. Coenzyme 4. Turbidometry 5. Streak plate

SECTION – B

Answer any five questions 5 x 3 = 15 M

6. Phospholipids 7. Paper chomatography 8. Cofactors 9. Factors influencing microbial growth 10. ED path way 11. Amino acids 12. Allosteric inhibition 13. Substrate level phosphorylation

SECTION – B Answer all the Questions. Draw labeled diagrams wherever necessary 5 x 8 = 40 M 14. a) Write an essay on general characteristics of carbohydrates.

(Or) b) Describe the structure of DNA.

15. a) Write in detail about the principle and applications of colorimerty. (Or)

b) Discuss about spectrophotometry.

16. a) Discuss the properties and classification of Enzymes. (Or)

b) Describe the process of biocatalysis.

17. a) Explain in detail about nutritional groups of microorganisms. (Or)

b) Explain different methods of measuring the microbial growth.

18. a) Write an essay on Glycolysis. (Or)

b) Discuss the fermentation processes.

I B.Sc., Microbiology - Practical Examinations Paper I model at the end of II Semester (w.e.f. 2017-18)

Introduction to Microbial Biochemistry and Metabolism (MB2209P)


1. Qualitative analysis of chemical ‘A’. 10 M

Scheme for valuation: Conduct of experiment (05M) + procedure (3M) + Result (2M)

2. Preparation of culture medium ‘B’. 10 M

Scheme for valuation: Principle (5M) + Procedure (5M)

3. Estimation of microbial growth ‘C’. 10 M

Scheme for valuation: Practical work (5M) + Description (5M)

4. Identify and write notes on the following 5 x 2 = 10 M D. Biochemical test E. Biochemical test F. Medium G. Isolation technique H. Instrument Scheme for valuation: Identification (1M) + Reasons (2M)

5. Record + Viva voce 6 +4 = 10 M --------------------------------------------------------------------------------------------------------------------- Total = 50 M ---------------------------------------------------------------------------------------------------------------------

II B.Sc. ,- Microbiology / III Semester End (W.E.F. 2015-2016)

Microbial Physiology and Genetics (Course code : MB 3209) Total Hrs. of Teaching: 52 @ 4 h / Week Total Credits : 03

Module – I Nutrition and growth 14 hours

a. Nutritional requirements and uptake of nutrients by cells.

b. Nutritional groups of microorganisms: Autotrophs, heterotrophs, mixotrophs,

methylotrophs.

c. Growth media : Synthetic, non-synthetic, selective, enrichment and differential

media.

d. Microbial growth : Different phases of growth in batch cultures; Factors influencing

microbial growth. Synchronous, continuous, biphasic growth.

e. Methods for measuring microbial growth : Direct microscopy, viable count

estimates, turbidometry, biomass.

Module – II Basics of Enzymes 12hours

a. Enzymes: Properties and classification, enzyme unit. Biocatalysis - induced fit, and

lock and key model, coenzymes, cofactors,

b. Factors affecting catalytic activity of enzymes.

c. Inhibition of enzyme activity - competitive, noncompetitive, uncompetitive

allosteric.

Module – III Principles of Genetics 14hours

a. Mendelian laws, alleles, crossing over and linkage.

b. DNA and RNA as genetic materials.

c. Watson and Crick model of DNA.

d. Replication of DNA : Semi conservative mechanism.

e. Outlines of DNA damage and repair mechanisms.

f. Extrachromosomal genetic elements : Plasmids and transposons.

Module – IV Mutations and recombination 11hours

a. Mutations: Spontaneous and induced, base pair changes, frame shifts, deletions,

inversions, tandem duplications, insertions.

b. Various physical and chemical mutagens.

c. Gene transfer among bacteria : Transformation, transduction and conjugation.

P.R.Government College (Autonomous), Kakinada II year B.Sc., Program III Semester End

Microbiology II A : Microbial Physiology and Genetics (Course Code MB 3209 w.e.f. 2015-2016)

Time : 3 hours Max. Marks:75

PART – A 5 x 2 = 10 M

Answer ALL of the following questions.

1. Acetogens

2. Co factor

3. Crossing over

4. Insertions

5. Chemostat

PART – B 5 x 4 = 20 M

Answer any FIVE of the following.

6. Growth curve

7. Synchronous culture

8. Lock and key hypothesis

9. Factors effecting catalytic activity of enzymes

10. Law of independent assortment

11. Transposons

12. Physical mutagens

13. Trasformation

PART – C 4 x 10 = 40 M

Answer any four of the following questions, Draw diagrams wherever necessary.

14 (a) Explain nutritional requirements of bacteria.

(OR)

(b) Write about growth media.

15. (a) Describe about Factors effecting catalytic activity of enzymes.

(OR)

(b) Give a brief account on Classification & nomenclature of Enzyme.

16. (a) Explain about Watson & Crick model of DNA.

(OR)

(b) Discuss in detail about different repair mechanism in DNA.

17. (a) What is mutation ? Describe various types of chemical mutations.

(OR)

(b) Give a brief account on Conjugation & Transduction

P.R.Government College (Autonomous), Kakinada II year B.Sc., Program IV Semester End

Microbiology II B : Microbial Physiology and Genetics (Course Code MB 4209 w.e.f. 2015-2016)

----------------------------------------------------------------------------------------------------------------

Module – I Metabolism -1 10hours

a. Common microbial fermentations : Alcohol and lactic acid fermentations. b. Photosynthetic apparatus in prokaryotes. c. Outlines of oxygenic and anoxygenic photosynthesis in bacteria.

Module – II Metabolism-2 15hours

a. Comparison of aerobic and anaerobic respirations b. Anaerobic respiration : Nitrogen and Sulphur c. Glycolysis, HMP pathway, ED pathway, TCA cycle, d. Electron transport, Oxidative and substrate-level phosphorylation. e. Anaplerotic reactions - Glyoxylate cycle f. β-oxidation of fatty acids.

Module – III Structure and control of gene 13 hour

a. Concept of gene : Muton, recon and cistron. One gene-one enzyme, one gene-one polypeptide, one gene-one product hypothesis.

b. Types of RNA and their functions. Outlines of RNA biosynthesis in prokaryotes. Genetic code; Structure of ribosomes and a brief account of protein synthesis.

c. Types of genes – structural, constitutive, regulatory.

d. Operon concept - Regulation of gene expression in bacteria – lac operon.

Module – IV r-DNA technology 14 hours

a. Restriction endonucleases, polymerases and ligases.

b. Different types of Vectors

c. Outlines of gene cloning methods.

d. Genomic and cDNA libraries; PCR technique

e. General account on application of genetic engineering in industry, agriculture and medicine.

P.R.Government College (Autonomous), Kakinada II year B.Sc., Program IV Semester End

Microbiology II B : Microbial physiology and genitics (Course Code MB 4209 w.e.f. 2015-2016)


PART – A 5 x 2 = 10 M

Answer ALL of the following questions.

1. Bacterial chlorophylls

2. Substrate level phosphorylation

3. Cistron

4. DNA ligase

5. Anoxygenic bacteria

PART – B 5 x 4 = 20 M

Answer any FIVE of the following

6. Lactic acid fermentation 7. Bacterial photosynthetic apparatus 8. ED pathway 9. β-Oxidation of fatty acids 10. Genetic code 11. Split genes 12. PCR technique 13. Restriction endonucleases

PART – C 4 x 10 = 40 M


14. (a) Write about lactic acid fermentation.

(OR)

(b) Write about the oxygenic photosynthesis in Bacteria.

15. (a) Write about HMP pathway.

(OR)

(b) Explain about the Electron Transport Chain.

16. (a) Write about different types and functions of RNA.

(OR)

(b) Describe the Lac-Operon concept.

17. (a) Write the different steps involved in gene cloning.

(OR)

(b) Give an account on application of genetic engineering in Agriculture

and Medicine.

II B.Sc., - Practical –II : MICROBIAL PHYSIOLOGY AND GENETICS

(Total hours of Laboratory exercises : 90 @ 3 hrs / week)

Minor experiments :

1. Preparation of media for culturing autotrophic and heterotrophic microorganisms –

algal medium, mineral salts medium, nutrient agar medium macconkey agar and

blood agar medium. (12 h)

2. Enrichment culturing and isolation of phototrophs and chemo-autotrophs (6 h)

3. Setting and observation of winogradsky column. (3 h)

4. Determination of viable count of bacteria. (3 h)

5. Turbidometric measurements of bacterial growth. (3 h)

6. Bacterial growth curve. (6 h)

7. Factors affecting bacterial growth – pH, temperature, salts. (9 h)

8. Starch hydrolysis, catalase test and sugar fermentation test. (9 h)

9. Qualitative analysis of sugars and amino acids. (21 h)

Major experiments :

10. Colorimetric estimation of DNA by Diphenyl amine method. (3 h)

11. Colorimetric estimation of protein by biuret/ Lowry’s method. (6 h)

12. Paper chromatographic separation of sugar and amino acid. (6 h)

13. Estimation of glucose by DNS method (3h)

Slides and spotters :

Apparatus, reagents, photos, medium, paper clippings (6 h)

II B.Sc., Microbiology Practical Examinations Paper II model at the end of IV Semester

Microbial Physiology and Genetics (MB 4209p)


1. Estimation of protein by Biuret method ‘A’. 20 M

Scheme for valuation : Practical work (10M) + Description (5M)

+ Result and discussion (5M)

2. Bacterial growth curve ‘B’. 10 M

Scheme for valuation : Principle (5M) + Procedure (5M)

3. Qualitative analysis of sugar ‘C’. 10 M Scheme for valuation : Biochemical tests (4M) + Osozone (2M)

+ Description (4M)

4. Identify and write notes on the following 5 x 3 = 15 M D. Medium

E. Equipment

F. Equipment

G. Reagent

H. Any reaction test

Scheme for valuation : Identification (1M) + Reasons (2M)

5. Viva voce 5 M

6. Record 10 M

---------------------------------------------------------------------------------------------------------------- Total = 70 M

CIA = 30 M

- --------------------------------------------------------------------------------------------------------------

Prescribed Text and Reference BooksPrescribed Text and Reference BooksPrescribed Text and Reference BooksPrescribed Text and Reference Books

II B.Sc., - Microbiology – PAPER-II

� Gottschalk, G. (1986). Bacterial Metabolism, Springer-Verlag, New-York.

� Caldwell, D.R. (1995). Microbial Physiology and Metabolism, W.C. Brown

Publications, Iowa, USA.

� Moat, A.G. and Foster, J.W. (1995). Microbial Physiology, John-Wiley, New

York.

� White, D. (1995). The Physiology and Biochemistry of Prokaryotes, Oxford

University Press, New York.

� Reddy, S.R. and Reddy, S.M. (2004). Microbial Physiology, Scientific Publishers,

Jodhpur, India.

� Reddy, S.M. and Reddy, S.R. (2005). A Text Book of Microbiology Vol-II.

Microbial Metabolism and Molecular Biology. Himalaya Publishing House,

Mumbai.

� Lehninger, A.L., Nelson, D.L. and Cox, M.M. (1993). Principles of Biochemistry,

2nd

Edition, CBS Publishers and Distributors, New Delhi.

� Elliot, W.H. and Elliot, D.C. (2001). Biochemistry and Molecular Biology, 2nd

Edition,

� Oxford University Press, U.S.A.

� Verma, P.S. and Agarwal, V.K. (2004). Cell Biology, Genetics, Molecular

Biology, Evolution and Ecology. S. Chand & Co. Ltd., New Delhi.

� Freifelder, D. (1997). Essentials of Molecular Biology. Narosa Publishing House,

New Delhi.

� Crueger, W. and Crueger, A. (2000). Biotechnology: A Text Book of Industrial

Microbiology, Prentice-Hall of India Pvt. Ltd., New Delhi.

III B.Sc. ,- Microbiology / V Semester End (W.E.F. 2016-2017)

Advanced Core : Immunology (Course Code : MB5217) Total Hrs. of Teaching: 45 @ 3 h / Week Total Credits : 03

Module - 1 Organs of Immunity 10hours

a. Historical events in development of immunology.

b. Immunity – innate and acquired; active and passive, humoral and cell-mediated.

c. Primary and secondary organs of immune system – thymus, bursa fabricus, bone marrow, spleen and lymph nodes; MALT and GALT

Module – II Cell mediated Immunity 10 hours

a. Limphoid cells : Identification and function of B and T lymphocytes, null cells.

b. Mononuclear phagocytes : Identification and functions of monocytes and macrophages

c. Granulocytic cells : Identification and functions of neutrophils, basophils, eosinophils, mast cells and dendritic cells .

Module – III Antigens & Antibodies 11 hours

a. Antigens : Types, chemical nature, antigenic determinants, haptens.

b. Factors affecting antigenicity.

c. Antibodies : Basic structure, types, properties and functions of immunoglobulins.

d. Components of complement and activation of complement.

Module – IV Interations of Antibodies & Antigens 14 hours

a. Types of antigen-antibody reactions : Agglutination, blood groups, precipitation, neutralization, complement fixation.

b. Labeled antibody based techniques: ELISA, RIA and Immunofluroscence.

c. Polyclonal and monoclonal antibodies : Production and applications.

d. Types of hypersensitivity : Immediate and delayed.

e. Autoimmunity and its significance.

P.R.Government College (Autonomous), Kakinada III year B.Sc., Program V Semester End

Advanced Core : IMMUNOLOGY (Course Code MB 5217 w.e.f. 2016-2017)


PART – A 5 x 2 = 10 M

Answer ALL of the following questions. 1. Spleen

2. Phagocytes

3. Hapten

4. Monoclonal antibodies

5. Physical barriers

PART – B 5 x 4 = 20 M


6. Thymus 7. Humoral immunity 8. Monocytes 9. Macrophages 10. Factors effecting antigenicity 11. Activation of component 12. Autoimmunity 13. Polyclonal antibodies

PART – C 4 x 10 = 40 M


14. (a) Write an essay on Acquired Immunity?

(OR)

(b) Write about the Secondary organs of Immune system?

15. (a) Give a brief account on lymphoid lineage.

(OR)

(b) Write an essay on Haematopoiesis with illustrations

16. a) Basic structure of Antibody and different types of antibodies?

(OR)

(b) What is Antigen? Explain the factors effecting Antigenicity

17. (a) Explain Antigen-Antibody reactions in vivo?

(OR)

(b) What is Hypersensitivity? Describe various types of Hypersensitivity

Advanced Core : Immunology (Course Code : MB5217p) Practical 1.Estimation of haemoglobin by Sahli’s method.

2. Estimation of haemoglobin by Cyname haemoglobin mehod.

3. Total count of W.B.C.

4. Total count of R.B.C.

5. Differential W.B.C. count.

6. Blood clotting factor

7. Erythrocyte Sedimentation Rate (ESR)

8. Flocculation reaction- VDRL

9. Agglutination reaction- Widal test, Blood Grouping.

10. Immuno-diffusion techniques- ODD.

11. Replica plating technique.

12. Estimation of skin microflora.

III B.Sc., Microbiology Practical Examinations Advanced Core : Immunology (Course Code : MB5217p)

at the end of V Semester

Time: 2 Hrs. Max. Marks: 50 M

1. Demonstrate the major experiment ‘A’ and write procedure and report results

12 M

Scheme for valuation : Practical work (4M) + Description (4M) + Result and discussion (4M)

2. Principle, procedure and inference of the given minor experiment ‘B’. 06 M Scheme for valuation : Principle (2M) + Procedure (2M) + inference (2M)

3. Identify and write notes on the following 3 x 4 = 12 M C. Organ of Immune system

D. Cell of Immunity

E. Antigen-antibody interaction

4. Record 05 M

---------------------------------------------------------------------------------------------------------------- Total = 35 M

CIA = 15 M

-----------------------------------------------------------------------------------------------------------

III B.SC. ,- MICROBIOLOGY / V SEMESTER END (W.E.F. 2016-2017)

Advanced Elective - 1 : Medical Microbiology

(Course Code : MB/MM5218A) Total Hrs. of Teaching: 30 @ 2 h / Week Total Credits : 02

Module - 1 Clinical Microbiology -1 6 hours

a. History of Medical Microbiology b. Normal micro flora of human body. c. Antagonism of indigenous flora. a. Anti-bacterial substances – lysozyme, complement, properdin. Antiviral

agents – interferon and base analogues.

Module – 2 Clinical Microbiology -2 6 hours

b. General principles of diagnostic microbiology. Collection, transport and processing of clinical samples.

c. General methods of laboratory diagnosis – cultural, biochemical, serological and molecular methods.

d. Tests for antimicrobial susceptibility. e. Bacterial toxins, virulence and attenuation.

Module - 3 Microorganisms & Diseases 13 hours

General account of the following diseases : Causal organisms, pathogenesis, epidemiology, diagnosis, prevention and control of:

a. Air-borne diseases - Tuberculosis, Influenza b. Food and water-borne diseases - Typhoid, Hepatitis-A, Amoebiasis c. Insect-borne diseases - Malaria, Dengue fever d. Contact diseases - Syphilis e. Zoonotic diseases - Rabies, Anthrax, f. Blood-borne diseases - AIDS

Module - 4 Control of Diseases 5 hours

a. Elements of chemotherapy ; Therapeutic drugs. Drug resistance. b. Mode of action of penicillin and sulpha drugs, and their clinical use.

c. Vaccines – natural and recombinant.


Advanced Elective-1 : MEDICAL MICROBIOLOGY (Course Code MB/MM 5218A w.e.f. 2016-2017)


PART – A 3 x 1 = 3 M

Answer any Three of the following. 1. Complement

2. Virulence

3. AIDS

4. PABA

5. Properdin

6. Recombinant vaccine

PART – B 4 x 4 = 16 M

Answer any Four of the following. Draw labeled diagrams wherever necessary. 7. Antibacterial substances

8. Bacterial toxins

9. Influenza

10. Drug resistance

11. Dengue fever

12. Antiviral agents

PART – C 2 x 8 = 16 M

Answer any Two of the following. Draw labeled diagrams wherever necessary. 13. Write about normal flora of Human body.

14. Write about general principles of Diagnostic Microbiology.

15. Give an account on food and water born diseases.

16. Explain mode of action of penicillin and sulpha drugs and their clinical uses.

Advanced Elective - I : Medical Microbiology

(Course Code : MB/MM5218P) Practical

1. Isolation and identification of Staphylococcus aureus

2. Isolation and identification of Escherichia coli

3. Isolation and identification of Streptococcus pyogenes

4. Isolation and identification of Pseudomonas

5. Isolation and identification of Klebsiella

6. Antibiotic sensitivity test by disc diffusion method

6. Phenol coefficient test for disinfectant

III B.Sc., Microbiology Practical Examinations Advanced Elective -1 : Medical Microbiology (MB/MM5218P)


Time: 2 Hrs. Max. Marks: 50 M 1. Demonstrate the major experiment ‘A’ and write procedure and report results

12 M Scheme for valuation : Practical work (4M) + Description (4M)

+ Result and discussion (4M) 2. Principle, procedure and inference of the given minor experiment ‘B’.

06 M

Scheme for valuation : Principle (2M) + Procedure (2M) + inference (2M)

3. Identify and write notes on the following 3 x 4 = 12 M C. Virus photograph/slide

D. Protozoa photograph/slide

E. Fungus photograph/slide

4. Record 05 M

---------------------------------------------------------------------------------------------------------------- Total = 35 M

CIA = 15 M

III B.SC. ,- MICROBIOLOGY / V SEMESTER END (W.E.F. 2016-2017)

Advanced Elective -2 : Food Microbiology

(Course Code : MB/FMB 5218B) Total Hrs. of Teaching: 30 @ 2 h / Week Total Credits : 02

Module – 1 Food Microbiology-1 6 hours

a. History and development of Food Microbiology.

b. Important micro organisms in food – Molds, yeast, viruses and Bacteria.

c. Sources of micro organisms in food – Plants, animals, water, sewage, air, soil, humans, food ingredients, equipment and miscellaneous.

Module -2 Food Microbiology-2 8 hours

a. Factors influencing microbial growth in foods.

b. Characteristics, mode of action and applications of LAB in foods.

d. Microbial food-borne diseases : Food intoxication (botulism and staph poisioning) food infections (salmonellosis and shigellosis) and their detection.


a. Spoilage of different food materials - fruits, vegetables, meat, fish, Canned foods.

b. Intrinsic and extrinsic factors in microbial food spoilage.

c. Food and enzymes produced by microbes.

d. Methods for the microbiological examination of foods.


a. Control of micro organisms in foods : Control by heat, low temperature and irradiation.

b. Food preservation using organic acids, diacetyl, Hydrogen peroxide and reuterine.

c. Fermented foods – cheese, yogurt


Advanced Elective-2 : FOOD MICROBIOLOGY

(Course Code MB/FMB 5218B w.e.f. 2016-2017) Time : 3 hours Max. Marks:70

PART – A 5 x 2 = 10 M

Answer Three of the following.

1. Robert Koch

2. Botulism

3. Rancidity

4. Chilling

5. Pectinases

PART – B 5 x 4 = 20 M

Answer any Four of the following. Draw labeled diagrams wherever necessary

6. Sources of food spoilage

7. Important molds in food

8. Salmonellosis

9. Applications of LAB in foods

10. Microbial spoilage of vegetables

11. Economically useful microbial enzymes

12. Yogurt

13. Control of microorganisms by heat

PART – C 4 x 10 = 40 M

Answer any Two of the following. Draw labeled diagrams wherever necessary

14 (a). Write an essay on important microbial organisms in foods. or (b) Explain different sources of food contamination.

15 (a).Discuss the factors influencing microbial growth in foods. or (b) Write an essay on food poisoning 16 (a).Write an essay different types of spoiled foods.

or (b) Briefly describe the microbiological observation of the foods.

17 (a).Discuss about anti microbial chemicals used in perseveration of foods.

or

(b) Explain the steps involved in production of different cheeses.

Advanced Elective - II : Food Microbiology

(Course Code : MB/FMB 5218B) Practical

1. Study of food spoilage microbes from sweets and bakery products.

2. Study of food spoilage microbes from sweets and bakery products.

3. Isolation of microbes from spoiled foods.

4. Determination of quality of milk by MBRT.

5. Study of the effect of food preservatives on the growth of microbes.

6. Quantitative analysis of milk by SPC

III B.Sc., Microbiology Practical Examinations Advanced Elective - 2 : Food Microbiology (MB/FMB 5218B)


Time: 2 Hrs. Max. Marks: 50 M 1. Demonstrate the major experiment ‘A’ and write procedure and report results


+ Result and discussion (4M) 2. Principle, procedure and inference of the given minor experiment ‘B’.

06 M

Scheme for valuation : Principle (2M) + Procedure (2M) + inference (2M)

3. Identify and write notes on the following 3 x 4 = 12 M C. Instrument for preservation

D. Industrial product

E. Food processing technique

4. Record 05 M

---------------------------------------------------------------------------------------------------------------- Total = 35 M

CIA = 15 M

SUGGESTED TEXT AND REFSUGGESTED TEXT AND REFSUGGESTED TEXT AND REFSUGGESTED TEXT AND REFERENCE BOOKSERENCE BOOKSERENCE BOOKSERENCE BOOKS

FOR III B.Sc., - MICROBIOLOGY – PAPER-III

Reddy, S.R. and Reddy, K.R. (2006) A Text Book of Microbiology - Immunology and Medical

Microbiology, Himalaya Publishing House, Mumbai.

Tizard, I.R. (1995) Immunology : An Introduction, WB Saunders, Philadelphia, USA.

Riott, I.M. (1998) Essentials of Immunology, ELBS and Black Well Scientific

Publishers, England.

Goldsby, Kindt, T.J. and Osborne, B.A. (2004) Kuby Immunology, 6th

Edition, W.H.Freeman and

Company, New York.

Lydyard, P.M., Whelan, A. and Fanger, M.W. (2000) Instant Notes in Immunology, Viva Books

Pvt. Ltd., New Delhi.

Chakraborty, B. (1998) A Text Book of Microbiology, New Central Book Agency (P) Ltd,

Calcutta, India.

Ananthanarayana, R. and Panicker, C.K.S. (2000) Text Book of Microbiology, 6th

Edition, Oriental

Longman Publications, USA.

Gupte, S. (1995) Short Text Book of Medical Microbiology, 8th

Edition, Jaypee Brothers Medical

Publishers (P) Ltd, New Delhi.

Annadurai, B. (2008) A Textbook of Immunology and Immunotechnology. S. Chand & Co. Ltd.,

New Delhi.

III B.SC. ,- MICROBIOLOGY / VI SEMESTER END (W.E.F. 2016-2017)

Skill based Core : Agricultural and Environmental Microbiology

(Courese Code : MB/AEM 6217) Total Hrs. of Teaching: 45 @ 3 h / Week Total Credits : 03

Module – 1 Microbes & Soil fertility 10 hours

a. Soil – a dynamic habitat of Microbes. b. Microbes in Rhizosphere and Phyllosphere. c. Plant growth - promoting microorganisms- Mycorrhizae, Rhizobia, Azospirillum,

Azotobacter, Cyanobacteria, Frankia.

d. Phosphate Solubilizing Microorganisms (PSM)

e. Outlines of biological nitrogen fixation (Symbiotic, Non-symbiotic).

Module -2 Microbes & Plant diseases 15 hours

a. A general account of different plant pathogens : Virus, Bacteria, Fungi, PPLO.

b. Symptoms, causal organism, disease cycle - environmental relations, management and control of following plant diseases:

ii. Viral : Bunchy top of Banana; Tungro of Rice

iii. Bacterial : Citrus canker; Bacterial blight of Rice

iv. Mycoplasma like organisms : Grassy shoot of Sugarcane; Little leaf of Brinjal

v. Fungi : Tikka disese of Ground nut; Blast of Rice; Red rot of Sugarcane.

c. Biological control of plant diseases. Biopesticides – Nuclear polyhedrosis virus (NPV), Bacillus thuringiensis, Pseudomonas fluorescens and Trichoderma virede.

Module – 3 Microbes in Environment 10 hours

a. Microorganisms of environment (soil, water and air). b. Role of microorganisms in nutrient cycling (Carbon, Nitrogen, Phophorus Sulphur).

c. Microbial interactions – mutualism, commensalism, antagonism, competition, parasitism, predation.

Module – 4 Microbes in Pollution Control 10 hours

a. Microbes in potable and polluted waters. E. coli and Streptococcus faecalis as indicators of water pollution.

b. Sanitation of potable water. Sewage treatment (primary, secondary and tertiary). Outlines of biodegradation of environmental pollutants – pesticides.

c. Solid waste disposal – sanitary land fills, composting.

d. Microbiology of air and air sampling methods.

P.R.Government College (Autonomous), Kakinada III year B.Sc., Program VI Semester End

Skill based Core : AGRICULTURAL AND ENVIRONMENTAL MICROBIOLOGY (Course Code MB/AEM 6217 w.e.f. 2016-2017)


PART – A 5 x 2 = 10 M

Answer Answer Answer Answer ALLALLALLALL of the folof the folof the folof the following questions.lowing questions.lowing questions.lowing questions.

1. PSM

2. NPV

3. Mutualism

4. Solid waste

5. Sanitory land fills

PART – B 5 x 4= 20 M


6. Rhizosphere 7. Azatobacter

8. Bacillus thuringiensis

9. PPLO

10. Microbes in water

11. Sulphur cycle

12. Primary sewage treatment

13. Biodegradation of Pesticides

PART – C 4 x 10 = 40 M

Answer any Answer any Answer any Answer any FOURFOURFOURFOUR of the following questions, Draw diagrams whereverof the following questions, Draw diagrams whereverof the following questions, Draw diagrams whereverof the following questions, Draw diagrams wherever necessary.necessary.necessary.necessary.

14. (a) Write an essay on Phosphate Solubilizing Microorganisms.

(OR)

(b) Discuss about symbiotic Nitrogen fixation.

15. (a) Write about the principles of plant disease control.

(OR)

(b) Write an essay on Blast of Rice.

16. (a) Write an essay on the role of microorganisms in carbon cycle.

(OR)

(b) Write an essay on the microorganisms of air and soil.

17. (a) Write an account of sewage treatment.

(OR)

(b) Write a note on the microbiology of potable water and describe the

process for sanitation of potable water

Skill based Core : Agricultural and Environmental Microbiology

(Course Code : MB/AEM 6217p)Practical

1. Isolation and enumeration of Rhizosphere microflora 2. Isolation and enumeration of Phyllosphere microflora 3. Isolation of Rhizobium from legume root nodules. 4. Isolation of Azospirillum and Azatobacter. 5. Staining and observation of VAM fungi. 6. Microbial examination of water by coli form test (Multiple tube fermentation method). 7. Determination of BOD of potable and polluted water samples. 8. Determination of COD of potable and polluted water samples 9. Observation of microbial diseases of locally important agricultural crops. 10. Production technology of biopesticides 11. Azolla cultivation 12. Isolation of Phosphate Solubilizing Microbes

III B.Sc., Microbiology Practical Examinations Skill based Core : Agriculture and Environmental Microbiology (AEM 6217p)

at the end of VI Semester



12 M

Scheme for valuation : Practical work (4M) + Description (4M) + Result and discussion (4M)


3. Identify and write notes on the following 3 x 4 = 12 M C. Medium

D. Disease

E. Biopesticide

4. Record 05 M

---------------------------------------------------------------------------------------------------------------- Total = 35 M

CIA = 15 M

---------------------------------------------------------------------------------------------------------------


Skill based Elective - 1 : Industrial Microbiology

(Course Code : MB/IM 6218A) Total Hrs. of Teaching: 30 @ 2 h / Week Total Credits : 02

Module - 1 Microbes in Industry 6 hours

a. Microorganisms of industrial importance : Yeasts, molds, bacteria, actinomycetes and micro algae.

b. Application of microbes in dairy and dairy products (cheese, yogurt, bread). c. Biofertilizers and Biopesticides

Module - 2 Microbes in Pharmacy 6 hours

a. Screening and isolation of industrially important microorganisms.

b. Outlines of strain improvement.

c. Role of transformed micro organisms in pharmaceuticals.

Module– 3 Industrial Microbiology -1 8 hours

a. Types of fermentation processes : Aerobic, anaerobic, batch, continuous, submerged and

surface.

b. Different types of fermentors

c. Design of a stirred tank fermentor

d. Fermentation media.

e. Control of agitation, aeration, pH, temperature and Dissolved Oxygen.

Module – 4 Indusrtial Microbiology -2 10 hours

a. Production of alcohols (ethyl alcohol), enzymes (amylase), antibiotics (penicillin), amino acids (glutamic acid),organic acids (citric acid), vitamins (B12), biofuels (biogas - methane).

b. Microorganisms as food : SCP, White button mushroom

c. Concept of probiotics.

P.R.Government College (Autonomous), Kakinada

III year B.Sc., Program VI Semester End

Skill based Elective-1 : INDUSTRIAL MICROBIOLOGY (Course Code MB / IM 6218A w.e.f. 2016-2017)

Time : 1½ hours Max. Marks : 35

PART – A 5 x 2 = 10 M

Answer All the following questions.

1. Yogurt

2. Crowded plate technique

3. Sparger

4. Probiotics

5. Impellor

PART – B 5 x 4 = 20 M

Answer any FIVE of the following. Draw labeled diagram wherever necessary 6. Microbes in cheese production

7. Strain improvement

8. Fermentation media

9. Biofuels

10. White button mushroom

11. Design of a stirred tank fermentor

12. Single Cell Proteins

13. Biogas

PART – C 4 x 10 = 40 M

Answer any Two of the following. Draw labeled diagram wherever necessary

14. Write an account on industrial importance of bacteria and actinomycetes.

(OR)

Discuss about bacterial biofertilizaers.

15. Describe briefly about screening technique.

(OR)

Write about the role of transformed micro organisms in pharmaceuticals.

16. Discuss about different fermentation techniques.

(OR)

Discuss about different types of fermentation media.

17. Write an essay on industrial production of citric acid using Aspergillus niger.

(OR)

Write an essay on production of Glutamic acid

Skill based Elective - 1 : Industrial Microbiology

(Course Code : MB/IM 6218A)

1. Quantitative estimation of alcohol by colorimetry

2. Alcohol fermentation by yeast

3. Isolation of Amylase producing organisms.

4. Estimation of citric acid by colorimetry.

5. Citric acid production by fermentation.

6. Immobilization of enzymes using Sodium alginate.

III B.Sc., Microbiology Practical Examinations Skill based Elective – 1 :Industrial Microbiology (IM 6218p)

at the end of VI Semester




+ Result and discussion (4M)


3. Identify and write notes on the following 3 x 4 = 12 M C. Single Cell Protein

D. Mold

E. Biofertilizer

4. Record 05 M

---------------------------------------------------------------------------------------------------------------- Total = 35 M

CIA = 15 M

- --------------------------------------------------------------------------------------------------------------


Skill based Elective-2 : Principles of Bioinstrumentation

(Course Code : MB/PB 6218B) Total Hrs. of Teaching: 30 @ 2 h / Week Total Credits : 02

Module – 1 Principles of instruments 7 Hours

i. pH meter ii. Conductivity meter

iii. Turbidometry iv. Flame photometer –

V. Spectrophotometer – UV & visible VI. Thermo cycler

Module – 2 Centrifugation 8 Hours

a. Basic principles of centrifugation ; types of rotors

b. Analytical and preparative centrifugation

c. Types of centrifuges- small bench, density gradient centrifugation, large capacity

refrigerated, high speed refrigerated, Ultracentrifuge.

Module – 3 Chromatography 8 Hours

i. Paper Chromatography ii. Thin Layer Chromatography

iv. Column Chromatography iv. Adsortption Chromatography

v. Ion exchange Chromatography VI.HPLC

Module –4Electrophoresis 7 Hours

a. Electrophoresis- Principles and applications.

b. SDS- PAGE, Agarose gel electrophoresis

c. Immuno electrophoresis.

P.R.Government College (Autonomous), Kakinada

III year B.Sc., Program VI Semester End

Skill based Elective-2 : PRINCIPLES OF BIOINSTRUMENTATION (Course Code MB/PB 6218B w.e.f. 2016-2017)


PART – A 3 x 1 = 3 M

Answer Answer Answer Answer any Threeany Threeany Threeany Three of the followingof the followingof the followingof the following. 1. pH

2. Centrifugation

3. Agarose

4. Stationary phase

5. TLC

6. Solvent

PART – B 4 x 4 = 16 M

Answer Answer Answer Answer any any any any FourFourFourFour of the following.of the following.of the following.of the following. Draw labeled diagrams wherever Draw labeled diagrams wherever Draw labeled diagrams wherever Draw labeled diagrams wherever necessary.necessary.necessary.necessary.

7. Flame photometer 8. Types of rotors

9. Adsorption chromatography

10. Applications of electrophoresis

11. Agarose gel electrophoresis

12. Ion exchange Chromatography

PART – C 2 x 8 = 16 M

Answer Answer Answer Answer any Twoany Twoany Twoany Two of the following.of the following.of the following.of the following. Draw labeled diagrams wherever Draw labeled diagrams wherever Draw labeled diagrams wherever Draw labeled diagrams wherever

necessary.necessary.necessary.necessary. 13. Write the principle and instrumentation of UV spectrophotometer.

14. Discuss about different types of centrifuges.

15. Write an essay on paper chromatography technique.

16. Give a brief account on Immuno electrophoresis.

Skill based Elective - 2 : Principles of bioinstrumentation

(Course Code : MB/PB 6218B)

1. Working principles and operation of pH meter and Conductivity meter

2. Working principles and operation of Turbidometer and Flame photometer

3. Working principles and operation of Spectrophotometer – UV & visible

4. Working principles and operation of Thermo cycler

5. Working principles and operation of different types of centrifuges.

6. Demonstration and operation of paper Chromatography

7. Demonstration and operation of Thin Layer Chromatography

8. Demonstration of SDS-PAGE Gel electrophoresis

III B.Sc., Microbiology Practical Examinations Skill based Elective – 2 : Principles of Bioinstrumentation

(MB/PB 6218B) at the end of VI Semester Time: 2 Hrs. Max. Marks: 50 M


12 M

Scheme for valuation : Practical work (4M) + Description (4M) + Result and discussion (4M


3. Identify and write notes on the following 3 x 4 = 12 M C. Instrument

D. Technique

E. Material

4. Record 05 M

---------------------------------------------------------------------------------------------------------------- Total = 35 M

CIA = 15 M

- ----------------------------------------------------------------------------------------------------------

List of paper setters and examiners For Microbiology

1 Sri. Trinadh, Lec. In-Charge, Dept. of Microbiology, Govt. Arts College(A), Rajahmundry, E.G.Dt.

2 Smt. T.Sony, Lec. In-Charge, Dept. of Microbiology S.C.I.M. Govt. Degree College, Tanuku, W.G.Dt.

3 Smt.DR.K.Aruna, Lecturer in Microbiology A.S.D. Govt. Degree College(W), Kakinada, E.G.Dt. 4 DR. Durga Prasad, Lecturer in Microbiology DR.V.S.Krishna Govt. Degree College (A), Visakhapatnam, VSP Dt.

5 Smt. Padmavathi, Lecturer in Microbiology St. Theresa’s Autonomous College (Women), Eluru, W.G.Dt.

6 Smt. Neeraja, Lecturer in Microbiology St. Theresa’s Autonomous College (Women), Eluru, W.G.Dt.

7 Sri K.Balachandra, Lecturer in Microbiology Andhra Loyola College (Autonomous), Vijayawada, Krishna Dist.

8 Smt. R.Shakunthala, Lecturer in Microbiology D.N.R.College (Autonomous), Bhimavaram, W.G.Dt.

9. Dr. K. Aruna, Lecturer in Microbiology A.S.D. Govt. Degree College for Women (Autonomous) Kakinada, E.G.Dt.

Suggested Student Project works

1. Influence of different sugars on the production of ethanol by yeast fermentation.

2. Influence of heavy metals on the growth of microorganisms.

3. Effect of medicinal plants on the bacterial growth.

4. Production of enzymes and influence of different media constituents on the enzyme

production.

5. Diversity of microorganisms in water samples of different areas.

6. Conventional methods to decrease the microbial loads.

7. Collection and identification of air born microorganisms in the college campus.

8. Efficacy of seaweed extracts on microbes.

9. Effect of Mangrove plant extracts on microbes.

10. Determination of milk quality by MBRT.

11. Antibiotic sensitivity test against mastitis in cattle.

12. Screening of animals and human beings against Brucellosis by RBPT and STAT.

13. Isolation and identification of Rhizobia from root nodules of some Fabaceae

members.

14. Collection and identification of cyanobacteria from Kakinada.

15. Isolation and identification of soil microbes in college campus.

16. A case study on phylloplane microflora of some plants in the college campus.

17. Blood grouping and its analysis in the students of PRGC (A), Kakinada.

P.R.GOVERMENT COLLEGE (AUTONOMOUS), KAKINADA – 533 001, A.P.

The Board of Studies in Microbiology for the Academic Year 2017–‘18 is conducted on 13-04-2017 at 10:00 A.M. in Dept. of Botany and Microbiology, P.R.G.C.(A), Kakinada.

Agenda

1. Modification of syllabus compared to previous year. 2. Department Action Plan for 2017-18. 3. New restructured course combinations as suggested by CCE. 4. Model question papers and Blue prints. 5. Panel of question paper setters and Examiners 6. Pedagogy implementation w.e.f. admitted batch 2017-18. 7. Additional inputs to curriculum. 8. Internal assessment weightage (40%). 9. Cluster electives/Subject electives.

10. End sem examination for 2½ Hrs. and 60 Marks. Mid semester examination for 1½ Hrs. and 40 Marks – w.e.f. 2017-18 admitted batch. 11. Conduct of semester wise practical examinations for 1st Year (2017-18 admitted batch). 12. Any other proposal with the permission of the Chair

The members of B.O.S. in Microbiology discussed all the issues kept in the agenda at length and taken following resolutions. RESOLUTIONS : 1. Resolved to approve the implementation of CCA for 40 marks and Semester end

evaluation for 60 marks in theory examinations w.e.f 2017-18 admitted batch. As per the direction of APSCHE and norms of ANUR, Rajamahendravaram University nominee differed and suggested to go for 75 and 25 respectively in respect of external and internal evaluation. As such the weightage of marks for I and II semester papers has to be changed.

2. It is resolved to approve 40% pass minimum in both theory and practicals. 3. Resolved to continue the implementation of CCA for 30 marks and Semester end

evaluation for 70 marks in theory examinations for II and III year (2016-17 & 2015-16 admitted batches).

4. Resolved to approve the syllabi of Advanced Core (Immunology) and Skill based core (Agricultural and Environmental Microbiology) courses offered respectively during V and VI Semesters.

5. Resolved to approve the syllabi of proposed Subject (discipline centric) Electives i.e., Advanced Electives (Medical Microbiology and Food Microbiology) during V semester and Skill based Electives (Industrial Microbiology and Principles of

Bioinstrumentation) during VI semester offered by the department w.e.f. 2016-2017 under CBCS pattern. Student has to chose one Advanced Elective (either of the 2 offered) in V semester and one Skill based Elective (either of the 2 offered) in VI semester.

6. Resolved to approve the syllabus and model question papers and blue prints for all the 6 semesters.

7. It is resolved to add 2 more practicals – (1) Immobilization of enzymes (2) Effect of pH and temperature on amylase activity.

8. It is resolved to shift the topic Concept of gene’ from Module – III in Semester IV to Module – III in Semester – III.

9. Resolved to conduct Semester wise practical exams for I and III year students. There is no internal assessment for I year students and practical exam is for 50 marks. For III year students CCA is 15 marks and semester end for 35 marks.

10. Resolved to conduct Year end practical exams for II year students. 11. Resolved to approve the panel of Paper setters and Examiners for Microbiology. 12. Resolved to approve the Action plan of the Department for the academic year 2017-

2018.

(DR.D.KALYANI) (DR.SMT.K.ARUNA) Assistant Professor Lec. in Microbiology & Subject Expert & ANUR Nominee A.S.D.G.D.C.(Autonomous) Dept. of Zoology Kakinada, East Godavari Dist. Adikavi Nannaya University Rajamahendravaram

(SMT.P.SWATHI) (DR.CH. USHA RANI) Assistant Director Principal Investigator Biological Control Laboratory DST-Disha (Women Scientist Project) Dept. of Agriculture Dept. of Botany and Microbiology Near R.T.O. Office, Kakinada Kakinada, E.G.Dt.

(DR. A. SRINIVASA RAO) Chairman, BOS in Microbiology Lec. In-Charge Dept. of Botany & Microbiology P.R.Govt. College (A), Kakinada

DEPARTMENT OF BOTANY AND MICROBIOLOGY ... CBCS 2017-2018.pdfDEPARTMENT OF BOTANY AND MICROBIOLOGY...

Documents

Transcript of DEPARTMENT OF BOTANY AND MICROBIOLOGY ... CBCS 2017-2018.pdfDEPARTMENT OF BOTANY AND MICROBIOLOGY...