Dengue Virus Virus, Epidemiology (Aedes aegypti vector) Dengue Fever (DF) versus Dengue Hemorrhagic...

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• Dengue Virus Virus, Epidemiology (Aedes aegypti vector) • Dengue Fever (DF) versus Dengue Hemorrhagic Fever (DHF) • Hypothesis Plasma biomarkers exist that can distinguish between DF and DHF early in disease progression • In search of…. Biomarkers = Prognostic Tools = Understanding Disease Pathogenesis In search of Dengue Biomarkers Student: Maike-Svenja Milkereit - Supervisor: Dr. Brian Ward, Montréal General Hospital

Transcript of Dengue Virus Virus, Epidemiology (Aedes aegypti vector) Dengue Fever (DF) versus Dengue Hemorrhagic...

Page 1: Dengue Virus Virus, Epidemiology (Aedes aegypti vector) Dengue Fever (DF) versus Dengue Hemorrhagic Fever (DHF) Hypothesis Plasma biomarkers exist that.

• Dengue VirusVirus, Epidemiology (Aedes aegypti vector)

• Dengue Fever (DF) versusDengue Hemorrhagic Fever (DHF)

• HypothesisPlasma biomarkers exist that can distinguish

between DF and DHF early in disease progression

• In search of….Biomarkers = Prognostic Tools= Understanding Disease Pathogenesis

In search of Dengue BiomarkersStudent: Maike-Svenja Milkereit - Supervisor: Dr. Brian Ward, Montréal General Hospital

Page 2: Dengue Virus Virus, Epidemiology (Aedes aegypti vector) Dengue Fever (DF) versus Dengue Hemorrhagic Fever (DHF) Hypothesis Plasma biomarkers exist that.

• Samples:– Disease (1o/2o, DF/DHF, time point collected, viral serotype)– Control

• Tools:– SELDI-TOF MS– CE & BPS Analysis– Biochemistry (Fractionations & Gels)

• Protein ID

• Biomarker Validation• Experiments Investigating Biomarker Role in Pathogenesis

Candidate

Biomarkers

In search of Dengue Biomarkers

?

Page 3: Dengue Virus Virus, Epidemiology (Aedes aegypti vector) Dengue Fever (DF) versus Dengue Hemorrhagic Fever (DHF) Hypothesis Plasma biomarkers exist that.

• Expected Results:– Candidate Biomarkers distinguishing DF/DHF/Control– Biomarker Identity: Host protein?

Immunoregulatory?

Affecting Vascular Endothelia?

M/Z

Signal Intensity

SELDI-TOF MS Spectra of Dengue Patient Plasma Sample (#35, 1DHFt1)

In search of Dengue Biomarkers

Thank-you!


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