Defining osteochondrosis and its genetic background · 2010-11-26 · Defining osteochondrosis and...
Transcript of Defining osteochondrosis and its genetic background · 2010-11-26 · Defining osteochondrosis and...
Defining osteochondrosis and its
genetic background
Ilse van GrevenhofAnimal Breeding and Genomics Centre
Bart Ducro
Piter Bijma
René van Weeren
Hans van Tartwijk
KWPN
What is OC?
Health problem in horses
Disturbance of ossification
In joints of young growing animals
Previous studies show prevalences vary 7% – 64%
Definition OC
Number of joints, radiographs, locations
Preselected data sample
Aims
Assess prevalences Joint level
Animal level
Stallion level
Study relationship Left and right joint
Flattenings and fragments
Genetic parameters Heritabilities
Genetic correlations
Materials
Dutch Warmblood population
2005/ 2006
OC was scored in 811 yearlings
After ‘point of no return’ (~12 months)
Randomly selected sample
Yearlings descended from 32 stallions
22 - 28 yearlings per stallion
Young and old stallions
Dressage and show jumping stallions
Methods
Scored on categorical scale A to E (Dik et al., 1999)
A = Ideal
B = Smooth flattened bone structure
C = Irregular flattened bone structure
D = Small bony fragment
E = Large bony fragment
Material and methods
OC scored on: 8 joints
14 radiographs
28 locations
Stifle Left 5 locations
Right 5 locations
Hock Left 7 locations
Right 7 locations
Fetlock 1 location
Methods: transformation to continuous scale
Aims: Estimate heritabilities and relationships
Problem: Observations are categorical
Needed: Transformation of categorical to continuous scale
Method: Based on prevalences
Result: 3 continuous traits FLAT
FRAG
ALL (FLAT + FRAG)
For each joint
For the entire animal (all joints combined)
Results
Animal level
Worst animal had 11 out of 28 ‘ideal’ locations
Best animal had 28 out of 28 ‘ideal’ locations
Joint Percentage of animals with fully ‘ideal’ joints
Stifle 61%
Hock 69%
Fetlock 65%
Total 30%
Results
Sire level
Mean: 30% of yearlings show fully ‘ideal’ joints
Worst stallion had 12% of yearlings with fully ‘ideal’ joints
Best stallion had 58% of yearlings with fully ‘ideal’ joints
No difference between show jumping/ dressage stallions
Are there prospects for breeding?
Sires (n=32)
Phe
noty
pic
SD
Standardized EBV of sires (All joints combined)
-0.470.33
~58%
~12%
Cluster analysis
Relationships
Reduction of variables
Based on correlation matrix
ALLHock
Front
Hind
Fetlock
Flat
Flat
Stifle
Frag
Front
Hind
Flat
FragR
L
R
L
R
Frag
L
R
L
R
L
L
L
R
L
R
R
1.0 0.20.6Proportion of variance explained
Cluster analysis
Clustering order:
Left and right joints most similar
FLAT and FRAG less similar
Joints least similar
Reduction from 16 to 6 variables
~0.63
Conclusions
Prevalences (% not ideal)
Varies between joints
Cluster analyses indicated:
Left and right joints can be combined in analyses
FLAT and FRAG should be separated in analyses
Large differences between stallions
Results hard to compare with literature
Definition OC
Number of joints, radiographs, locations
Statistical model
OC = µ + sex + vet + age + year + animal + e
OC is the continuous FLAT, FRAG or ALL for a joint or the entire animal
REsidual Maximum Likelihood (REML)
Pedigree N = 7799
Heritabilities
Joint h2 ALL (SE)
h2 FLAT (SE)
h2 FRAG (SE)
Animal 0.23 (0.09) 0.08 (0.06) 0.22 (0.09)
Stifle joint 0.05 (0.05) 0.07 (0.06) 0.02 (0.04)
Hock joint 0.36 (0.11) 0.15 (0.08) 0.26 (0.09)
Fetlock
joints
0.14 (0.08) 0.08 (0.10) 0.06 (0.07)
Heritabilities
Do we need to score left and right joints?
Heritabilities of left and right joints were highly similar
Combined h2 is higher (increase with 16%)
Do we need to score from A-E or will binary (0/1) do?
h2 entire animal: 0.23 0.15
Genetic correlations
Genetic correlation in entire animal
FLAT and FRAG was 0.80 (± 0.25)
Genetic correlations between joints
Stifle and hock joints: 0.59
Fetlock and other joints: 0.09 - 0.26
Which joints are relevant for breeding?
Stifle joint
High prevalence
High clinical relevance (low prognosis surgical treatment)
High SE for genetic correlation with hock joint (0.59 0.37)
Hock joint
High prevalence of fragments
High heritable
Fetlock joint
Intermediate heritable
Low genetic correlations (no correlated response)
Are both FLAT and FRAG relevant for breeding?
FLAT and FRAG
Although rg between FLAT and FRAG is high (0.80)
SE is large (0.25)
Heritabilities are substantially different
What can be concluded?
For breeding, OC should be recorded in:
All 4 joints
Flattenings and fragments
Left and right joints
Scored in >2 categories
Linear OC value
0
0,1
0,2
0,3
0,4
-3 -2 -1 0 1 2 3
y-axis: probability
x-axis: standard deviation
part belonging to
percentage of
category A
A
B
CD E
-0.23
Locations Stifle
1. Lateral femoral trochlea
2. Medial femoral trochlea
3. Sulcus distal femur
4. Patella
5. Other locations
Hock 1. Sagittal ridge of distal tibia
2. Lateral trochlea of talus
3. Medial trochlea of talus
4. Lateral malleolus of tibia
5. Medial malleolus of tibia
6. Base of talus
7. Other locations
Fetlock Proximodorsal part of the sagittal ridge of 3rd metacarpal/metatarsal bone
Radiographs
Stifle
Lateromedial
Anterior-posterior lateral oblique
Hock
Anterior-posterior
Lateromedial
Anterior-posterior lateral oblique
Fetlock
Lateromedial