December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen...
Transcript of December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen...
![Page 1: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/1.jpg)
December 9, 2014
Guido Breuer
Bioprocess Engineering & AlgaePARC, Wageningen University, The
Netherlands
Microalgae for a bio-based economy
![Page 2: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/2.jpg)
Bioprocess Engineering & AlgaePARC
Wageningen University Research Centre
Food and
Biobased
Research
(FBR)
Bioprocess
Engineering
(Prof. René
Wijffels)
• ± 20 Staff members• ± 30 PhD students• MSc & BSc students• Different research topics of which algae are an important theme
![Page 3: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/3.jpg)
Our algae research
Pilot-scale (outdoor)
Lab-scale
Various products
In close collaboration with industry
Lab-scale (indoor) and pilot-scale (outdoor)
Fundamental research and applied research
Multidisciplinary research
Whole algae production chain (from strains to product)
![Page 4: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/4.jpg)
Multidisciplinary algae production chain
Strain selection and improvement
Physiology Cultivation Harvesting Bio-refinery
• Strain
selection
(screening)
• Genetic
engineering
• Photosynthetic
efficiency
• Product
accumulation
• Day-night
cycles
• Flocculation • Cell disruption
• Fractionation
• Cell wall
characterization
Life cycle and techno-economic analysis, product characterization
• Photobioreactor
design
• Process design
• Cultivation
strategies
• Scale-up
• Optimization
![Page 5: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/5.jpg)
AlgaePARC pilot facilities
4 reactor designs
25 m2
Different cultivation modes
Natural seawater
![Page 6: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/6.jpg)
Production systems at AlgaePARC (1)
Raceway pond Horizontal tubular reactor
Advantage- Cheap to build and to operate- Established technology
Disadvantages- Prone to contamination- Water evaporation- Controllability- Low biomass concentrations
Advantage- High controllability- Closed system- High biomass concentrations
Disadvantages- Photo inhibition- Oxygen built up
![Page 7: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/7.jpg)
Production systems at AlgaePARC (2)
Flat panels Vertical stacked tubular reactor
Advantage- Low material costs- No need for external cooling- High biomass concentrations
Disadvantages- Prone to damage- Energy costs of air sparging
Advantage- High controllability- Closed system- Light dilution
Disadvantages- Oxygen built up- Costs/energy to built
![Page 8: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/8.jpg)
Aims of AlgaePARC
Comparison of photobioreactor designs
Improve photosynthetic efficiency outdoors (>5%)
Develop process & cultivation strategies
Obtain knowledge and experience to scale-up
Growth model development and validation
Productivity projections
LCA and techno-economic analysis
Bosma et. al. 2014, Algal. Res.
![Page 9: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/9.jpg)
Biorefinery
Postma et. al. 2014, Biores. Technol.
Salim et al. 2011 J. appl. Phycol.
![Page 10: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/10.jpg)
Strains selection and improvement
Model species and industrially relevant species
Photosynthesis and product formation
● Antennae mutants
● TAG production (starchless mutants)
● Hydrocarbon production
Mutagenesis and direct gene targetingapproaches
(Transcript)omics to obtain insight in regulation
![Page 11: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/11.jpg)
Photobioreactor design
Foam
Biofilms
![Page 12: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/12.jpg)
Thank you!
![Page 14: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/14.jpg)
Biomass Production costs: Model
NetherlandsSaudi ArabiaCanary IslandsTurkish RivieraSouth SpainCuracao
Culture temperatureDaily Dilution
Mixing day/night)Operation days per year
...
Light IntensityElectricity costs
TaxesLabor
€ / Kg biomass
CAPEX & OPEX
NER
SensitivityAnalysis
Areas to focus
Output
Location
CultivationSystem
Empirical data
Specificparameters
Input
![Page 15: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/15.jpg)
Selection of oil producting species
Breuer et al. 2012, Biores. Technol.
Nitrogen starvation to induce oil production
Many oil producing species exist
More than 40% of DW as oils
![Page 16: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/16.jpg)
Strain improvement: starchless mutants
TAP -N
Random UV-mutagenesis
Screening using iodine staining
De Jaeger et. al. 2014, Biotech. For Biofuels
![Page 17: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/17.jpg)
Starchless mutants: biomass composition
Breuer & de Jaeger et. al. 2014, Biotech. For Biofuels
![Page 18: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/18.jpg)
Day:night cycles
Light intensity is variable during day:night cycle
Use reactor design for light dilution
Wijffels & Barbosa 2010, Science
Cuaresma et al. 2011, Biores. Technol.
Different dilution rates
Not just light intensity changes during day:night cycles
![Page 19: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/19.jpg)
Yield and biomass composition change during cell cycle
De Winter et. al. 2013, Algal Res.
De Winter et. al. 2014, J. of Biotech.
Constant light intensity but cell-cycle-synchronized culture
![Page 20: December 9, 2014 Guido Breuer breuer.pdf · Bioprocess Engineering & AlgaePARC, Wageningen University, The Netherlands Microalgae for a bio-based economy. ... •Flocculation Cell](https://reader030.fdocuments.in/reader030/viewer/2022040905/5e7a4261925f91708914ff1c/html5/thumbnails/20.jpg)
Starchless mutants: performance
50% higher yield
Breuer & de Jaeger et. al. 2014, Biotech. For Biofuels