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DDominiqueominiqueDDelteilelteilCConsultant onsultant


CoinoculationCoinoculation and good practices for managing MLF, and good practices for managing MLF, fruit, longevity and prevention of microbial spoilage (such fruit, longevity and prevention of microbial spoilage (such

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Documento Dominique Delteil Consultant.Impiego vietato per uso professionale (formazione, riproduzione, articoli, documentazione commerciale, ecc.) senza l’accordo scritto di Dominique Delteil

fruit, longevity and prevention of microbial spoilage (such fruit, longevity and prevention of microbial spoilage (such as as BrettanomycesBrettanomyces) in red wines) in red wines

赤ワインにおけるコ・イノキュレーションと赤ワインにおけるコ・イノキュレーションとMLFMLF管理手順管理手順果実香、長期品質維持、変敗予防（ブレタノマイセス）果実香、長期品質維持、変敗予防（ブレタノマイセス）



Testimonial of my R&D and consulting Testimonial of my R&D and consulting

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Testimonial of my R&D and consulting Testimonial of my R&D and consulting experience with experience with coinoculationcoinoculation yeastyeast--bacteriabacteria私の研究開発とコンサルティング業務の経験から私の研究開発とコンサルティング業務の経験からコ・イノキュレーションをお勧めしますコ・イノキュレーションをお勧めします



1.1.1.1.What is yeast bacteria What is yeast bacteria

coinoculation?coinoculation?酵母乳酸菌の酵母乳酸菌の

コ・イノキュレーションとは何かコ・イノキュレーションとは何か


コ・イノキュレーションとは何かコ・イノキュレーションとは何か



The winemaker visionThe winemaker visionワインメーカーの視点ワインメーカーの視点




Brix and malic curves during a coBrix and malic curves during a co--inoculated yeast inoculated yeast -- bacteria fermentationbacteria fermentationコ・イノキュレーション中のコ・イノキュレーション中のBirxBirxとリンゴ酸の推移とリンゴ酸の推移

Yeast inoculationYeast inoculation 酵母接種酵母接種Yeast inoculationYeast inoculation 酵母接種酵母接種

As soon as the pomace cap is formed, As soon as the pomace cap is formed, coinoculationcoinoculationwith selected lactic acid bacteriawith selected lactic acid bacteria果帽形成直後に選抜乳酸菌をコ・イノキュレーション果帽形成直後に選抜乳酸菌をコ・イノキュレーション

As soon as the pomace cap is formed, As soon as the pomace cap is formed, coinoculationcoinoculationwith selected lactic acid bacteriawith selected lactic acid bacteria果帽形成直後に選抜乳酸菌をコ・イノキュレーション果帽形成直後に選抜乳酸菌をコ・イノキュレーション


1 week1 week1 week1 week BrixBrixBrixBrixMalicMalicacidacidMalicMalicacidacid



Two key winemaking actionsTwo key winemaking actions22つのキーアクションつのキーアクション


As soon as the pomace cap is formed, As soon as the pomace cap is formed, coinoculation coinoculation with selected LABwith selected LAB果帽形成直後に選抜乳酸菌をコ・イノキュレーション果帽形成直後に選抜乳酸菌をコ・イノキュレーション

As soon as the pomace cap is formed, As soon as the pomace cap is formed, coinoculation coinoculation with selected LABwith selected LAB果帽形成直後に選抜乳酸菌をコ・イノキュレーション果帽形成直後に選抜乳酸菌をコ・イノキュレーション

亜硫酸添加

SO2additionRacking

滓引き


BrixBrixBrixBrixMalicMalicacidacidMalicMalicacidacid

滓引き

1 week1 week1 week1 week



Sensory goals of coinoculation during Sensory goals of coinoculation during fermentationfermentationコ・イノキュレーション発酵中に目指す香味質コ・イノキュレーション発酵中に目指す香味質

Yeast inoculationYeast inoculationYeast inoculationYeast inoculation

As soon as the pomace cap is formed, As soon as the pomace cap is formed, coinoculation with selected LABcoinoculation with selected LABAs soon as the pomace cap is formed, As soon as the pomace cap is formed, coinoculation with selected LABcoinoculation with selected LAB

SO2addition


BrixBrixBrixBrixMalicMalicacidacidMalicMalicacidacid

additionRacking




Sensory goals of coinoculation during agingSensory goals of coinoculation during agingコ・イノキュレーション後、熟成中に目指す香味質コ・イノキュレーション後、熟成中に目指す香味質


As soon as the pomace As soon as the pomace cap is formed, cap is formed, As soon as the pomace As soon as the pomace cap is formed, cap is formed, cap is formed, cap is formed, coinoculation with coinoculation with selected LABselected LAB果帽形成直後に培養乳酸菌を果帽形成直後に培養乳酸菌をコ・イノキュレーションコ・イノキュレーション

cap is formed, cap is formed, coinoculation with coinoculation with selected LABselected LAB果帽形成直後に培養乳酸菌を果帽形成直後に培養乳酸菌をコ・イノキュレーションコ・イノキュレーション


1 month1 month1 month1 month

BrixBrixBrixBrix

Malic acidMalic acidMalic acidMalic acid

Aging 熟成



The microbiologist visionThe microbiologist vision微生物学者の視点微生物学者の視点




Population curves during a coPopulation curves during a co--inoculated inoculated yeast yeast -- bacteria fermentationbacteria fermentationコ・イノキュレーション中の酵母・乳酸菌コ・イノキュレーション中の酵母・乳酸菌菌数推移菌数推移


Lactic acid bacteria (LAB) coinoculationLactic acid bacteria (LAB) coinoculation乳酸菌コ・イノキュレーション乳酸菌コ・イノキュレーションLactic acid bacteria (LAB) coinoculationLactic acid bacteria (LAB) coinoculation乳酸菌コ・イノキュレーション乳酸菌コ・イノキュレーション

亜硫酸添加

SO2addition

乳酸菌コ・イノキュレーション乳酸菌コ・イノキュレーション乳酸菌コ・イノキュレーション乳酸菌コ・イノキュレーション

Yeast living populationYeast living populationYeast living populationYeast living population

LAB living populationLAB living population 乳酸菌生菌数乳酸菌生菌数LAB living populationLAB living population 乳酸菌生菌数乳酸菌生菌数



BrixBrixBrixBrix

MalicMalicacidacidMalicMalicacidacid

Yeast living populationYeast living population酵母生菌数酵母生菌数Yeast living populationYeast living population酵母生菌数酵母生菌数



Population curves during a coPopulation curves during a co--inoculated inoculated yeast yeast -- bacteria fermentationbacteria fermentationコ・イノキュレーション中の菌数推移コ・イノキュレーション中の菌数推移亜硫酸添加

SO2addition

LAB living populationLAB living population乳酸菌生菌数乳酸菌生菌数LAB living populationLAB living population乳酸菌生菌数乳酸菌生菌数


Yeast inoculationYeast inoculation酵母接種酵母接種Yeast inoculationYeast inoculation酵母接種酵母接種


乳酸菌生菌数乳酸菌生菌数乳酸菌生菌数乳酸菌生菌数

LAB coinoculationLAB coinoculation乳酸菌コ・イノキュレーション乳酸菌コ・イノキュレーション




Indigenous Lactic Acid Indigenous Lactic Acid Bacteria living populationBacteria living population野性乳酸菌生菌数野性乳酸菌生菌数

Indigenous Lactic Acid Indigenous Lactic Acid Bacteria living populationBacteria living population野性乳酸菌生菌数野性乳酸菌生菌数



Post alcoholic fermentation LAB inoculation Post alcoholic fermentation LAB inoculation : indigenous LAB: indigenous LABアルコール発酵後の乳酸菌接種：野性乳酸菌アルコール発酵後の乳酸菌接種：野性乳酸菌

LAB inoculation, LAB inoculation, LAB inoculation, LAB inoculation,

SO2

addition

Selected LAB living populationSelected LAB living population 選抜乳酸菌数選抜乳酸菌数Selected LAB living populationSelected LAB living population 選抜乳酸菌数選抜乳酸菌数



LAB inoculation, LAB inoculation, post AFpost AFアルコール発酵後の乳アルコール発酵後の乳酸菌接種酸菌接種

LAB inoculation, LAB inoculation, post AFpost AFアルコール発酵後の乳アルコール発酵後の乳酸菌接種酸菌接種



1 week1 week1 week1 weekIndigenous Lactic Acid Indigenous Lactic Acid Bacteria living populationBacteria living population野性乳酸菌数野性乳酸菌数

Indigenous Lactic Acid Indigenous Lactic Acid Bacteria living populationBacteria living population野性乳酸菌数野性乳酸菌数

Reminder: Indigenous Lactic Acid Bacteria living population with yeast Reminder: Indigenous Lactic Acid Bacteria living population with yeast --bacteria bacteria coinoculationcoinoculationReminder: Indigenous Lactic Acid Bacteria living population with yeast Reminder: Indigenous Lactic Acid Bacteria living population with yeast --bacteria bacteria coinoculationcoinoculation

コ・イノキュレーション時のコ・イノキュレーション時の野性乳酸菌数野性乳酸菌数



Brettanomyces sp.Brettanomyces sp. population curves during a copopulation curves during a co--inoculated inoculated yeast yeast -- bacteria fermentationbacteria fermentationコ・イノキュレーション中のコ・イノキュレーション中のブレタノマイセス菌数推移ブレタノマイセス菌数推移

亜硫酸添加

SO2addition

LAB living populationLAB living population乳酸菌生菌数乳酸菌生菌数LAB living populationLAB living population乳酸菌生菌数乳酸菌生菌数


Yeast inoculationYeast inoculation酵母接種酵母接種Yeast inoculationYeast inoculation酵母接種酵母接種


乳酸菌生菌数乳酸菌生菌数乳酸菌生菌数乳酸菌生菌数





Indigenous Indigenous Brettanomyces sp.Brettanomyces sp.living populationliving populationブレタノマイセス生菌数ブレタノマイセス生菌数

Indigenous Indigenous Brettanomyces sp.Brettanomyces sp.living populationliving populationブレタノマイセス生菌数ブレタノマイセス生菌数



Post alcoholic fermentation LAB Post alcoholic fermentation LAB inoculation: Brett !inoculation: Brett !アルコール発酵後の乳酸菌接種；ブレット！アルコール発酵後の乳酸菌接種；ブレット！


SO2

addition




LAB inoculation, LAB inoculation, post AFpost AFアルコール発酵後の乳アルコール発酵後の乳酸菌添加酸菌添加



Brettanomyces sp.Brettanomyces sp. LivingLivingBrettanomyces sp.Brettanomyces sp. LivingLiving


1 week1 week1 week1 week Brettanomyces sp.Brettanomyces sp. LivingLivingpopulationpopulationブレタノマイセス属生菌数ブレタノマイセス属生菌数

Brettanomyces sp.Brettanomyces sp. LivingLivingpopulationpopulationブレタノマイセス属生菌数ブレタノマイセス属生菌数

Reminder: Reminder: BrettanomycesBrettanomyces living population living population with yeast with yeast -- bacteria bacteria coinoculationcoinoculationReminder: Reminder: BrettanomycesBrettanomyces living population living population with yeast with yeast -- bacteria bacteria coinoculationcoinoculation

コ・イノキュレーション中のコ・イノキュレーション中のブレタノマイセス属生菌数ブレタノマイセス属生菌数



Post alcoholic fermentation LAB Post alcoholic fermentation LAB inoculation: Brett !inoculation: Brett !アルコール発酵後の乳酸菌接種；ブレット！アルコール発酵後の乳酸菌接種；ブレット！


SO2

addition







Brettanomyces sp.Brettanomyces sp.Brettanomyces sp.Brettanomyces sp.


1 week1 week1 week1 week Brettanomyces sp.Brettanomyces sp.Living populationLiving populationブレタノマイセス属生菌数ブレタノマイセス属生菌数

Brettanomyces sp.Brettanomyces sp.Living populationLiving populationブレタノマイセス属生菌数ブレタノマイセス属生菌数

Reminder: Reminder: BrettanomycesBrettanomyces living population living population with yeast with yeast -- bacteria bacteria coinoculationcoinoculationReminder: Reminder: BrettanomycesBrettanomyces living population living population with yeast with yeast -- bacteria bacteria coinoculationcoinoculation

コ・イノキュレーション中のコ・イノキュレーション中のブレタノマイセス属生菌数ブレタノマイセス属生菌数



As a sensory consequence, with As a sensory consequence, with coinoculationcoinoculationコ・イノキュレーション後の香味コ・イノキュレーション後の香味 SO2

addition

LAB living populationLAB living populationLAB living populationLAB living population



LAB coinoculationLAB coinoculationLAB coinoculationLAB coinoculation


LAB living populationLAB living populationLAB living populationLAB living population





As a sensory consequence, with postAs a sensory consequence, with post--AF AF inoculationinoculationアルコール発酵後に乳酸菌接種の場合、香味は？アルコール発酵後に乳酸菌接種の場合、香味は？


SO2

addition

Selected LAB living Selected LAB living populationpopulationSelected LAB living Selected LAB living populationpopulation



LAB inoculation, LAB inoculation, post AFpost AFLAB inoculation, LAB inoculation, post AFpost AF




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