David et al, 2001tishreen.edu.sy/sites/default/files/Tishreen_Magazine/6_17.pdf · Monodonta...

Tishreen University Journal. Bio. Sciences Series

٨١

Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (30) No. (5) 2008

��

Cd, Pb, Cr, Cu, Zn

Pinctada radiataBrachidonta

variabilisPatella caeruleaMonodonta turbinataEriphia verrucosa

Cd, Pb

.w.wPinctada radiata

.w.wBrachidonta variabilis

.w.wPatella caeruleaMonodonta turbinata

.w.w

Patella

caerulea

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٨٢

Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (30) No. (5) 2008

Trace Heavy Metals Accumulation in Some Zoo Benthic Species of Banias Littoral Thermal Station

Dr. Fayez Saker* Dr. Mohamad.S. AL- Masri **

Mohamad Saleh ***

(Received 28 / 7 / 2008. Accepted 17/11/ )

� ABSTRACT �

We have studied the accumulation of elements (Cd, Pb, Cr, Cu, Zn) in five different

Zoo benthic species collected from Banias Littoral Thermal station. These species are: Pinctada radiata, Brachidonta variabilis, Patella caerulea, Monodonta turbinate and Eriphia verrucosa. Statistic results indicate a heavy metals accumulation in all organisms, and the differences were due to species difference and time and place of sampling. We can consider all studied species as indicators of pollution (Cd, Pb) and maximum limits between (Pb:2.43-4.80, Cd:1.05-2.16 µg/g.w.w). We can consider Pinctada radiata as bio indicators for Zinc whose limits reach to (875 µg/g.w.w) and Brachidonta variabilis bio indicators of pollution in Cooper (maximum limits 23.15µg/g.w.w). The two species: Patella caerulea and Monodonta turbinata are bio indicators for pollution in Chromium, its maximum value (4.60µg/g, 7.46µg/g.w.w). This study showed different types of correlation between elements, and the highest was between Cu and Zn in species Patella caerulea.

Keywords: Zoo benthos, bio indicators, Trace heavy metals.

* professor , Department of Zoology, Faculty of Science, Tishreen University , Lattakia , Syria

** Corresponding, Department of protection and safety, Atomic Energy Commission of Syria. *** Postgraduate student, High Institute of Marine Research - Department of Zoology, Faculty of Science, Tishreen University- Lattakia - Syria



٨١

UNEP,1995Usero,

(Islam et al,

2004)et al,Watzin

Mussel Watch

Bivalvia

MytilidaeOstriedae

Beldi et al, 2006

David et al, 2001

UNEP,1997

Cd,Pb,Cr,Cu,Zn

Bivalvia GastropodaCrustasea

٨٣


٨٤

A

B

C



٨٥

D

pietro , 1974,1976Parenzan,1974,1976 Gaillard , 1978 Riedl

,1983

Leachi,1814Pinctada radiataKrauss,1962Brachidonta variabilis

Bivalaia

Linnaeus,1758Patella caeruleaBorn,1780Monodonta turbinate

Gastropoda

Forskale,1775Eriphia verrucosaCrustacea

Arthropoda

UNEP,1984

Wet –Digestion Method

Hanson,

Atomic Absorption SpectrometerVarianAnalytic Jena AA

novFlame-AASZn , CuETA – AAS

Cd, Pb, Cr

,Trace

elements and methylmercury in fish tissue ,2003 IAEA-407

REFERENCE MATERIAL

Cd, Pb, Cr, Cu, Zn

ACD

A

A


٨٦

A

w.wµg/gPinctada radiate

w.wµg/g

Brachidonta variabilisw.wµg/g

Eriphia verrucosaPatella caeruleaMonodonta Turbinata

µg/g w.w

0

100

200

300

400

500

600

700

800

900

(µg

/g.w

.wز(

ك��یتر

ال

Patella

verrucosa

Monodonta

Turbinata

Pinctada

radiata

Brachidonta

variabilus

Eriphia

verrucosa

األنواع المدروسة A في الموقع Zn تركیز

20/6/2006 3/8/20065/10/2006 25/11/200620/1/2007 15/3/200722/5/2007 2/7/2007

Znµg/g.w.wA

Pinctada radiata

w.wµg/gBrachidonta variabilis

w.wµg/g

Bivalvia

Monodonta Turbinata

w.wµg/gverrucosaEriphia

Patella caeruleaw.wµg/g



٨٧

0

2

4

6

8

10

12

14

16

18

20

(µg

/g.w

.wز (

ك�یتر

ال

Patella

verrucosa

Monodonta

Turbinata

Pinctada

radiata

Brachidonta

variabilus

Eriphia

verrucosa

تركیز Cu في الموقع A األنواع المدروسة

20/6/2006 3/8/2006 5/10/200625/11/2006 20/1/2007 15/3/200722/5/2007 2/7/2007

Cuµg/g.w. wA

MonodontaTurbinataw.wµg/g

w.wµg/gPinctada radiata

0

1

2

3

4

5

6

7

(µg

/g.w

.wز(

كیتر

ال

Patella

verrucosa

Monodonta

Turbinata

Pinctada

radiata

Brachidonta

variabilus

Eriphia

verrucosa

األنواع المدروسة A في الموقع Cr تركیز

20/6/2006 3/8/2006

5/10/2006 25/11/2006

20/1/2007 15/3/2007

22/5/2007 2/7/2007

µg/g.w. w)Cr A

w.wµg/gBrachidonta variabilis

Monodonta Turbinata5

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

5

(µg

/g.w

.wز(

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata

Brachidontavariabilus

Eriphia verrucosa

األنواع المدروسة A في الموقع Pb تركیز

20/6/20063/8/20065/10/2006

25/11/2006

20/1/200715/3/200722/5/2007

2/7/2007

5µg/g.w. w)PbA


٨٨

-A

w.wµg/g Monodonta Turbinata

µg/g w.wPinctada radiata

Patella caerulea Brachidonta variabilis Eriphia verrucosa

µg/g w.w

0

0.5

1

1.5

2

2.5

(µg

/g.w

.wز(

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

تركیز Cd في الموقع A األنواع المدروسة

20/6/2006 3/8/2006

5/10/2006 25/11/2006

20/1/2007 15/3/2007

22/5/2007 2/7/2007

µg/g.w. w)CdA

B

C

C

0

100

200

300

400

500

600

700

800

900

(µg

/g.w

.wز (

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروسة C في الموقع Zn تركیز

20/6/2006

3/8/2006

5/10/2006

25/11/2006

20/1/2007

15/3/2007

22/5/2007

2/7/2007

µg/g.w. w) ZnC

A

Pinctada radiataw.wµg/g

wwµg/g



٨٩

Monodonta Turbinata Patella caerulea Brachidonta variabilis

verrucosaEriphia

Pinctada radiata

w.wµg/g

Monodonta Turbinataw.wµg/gBrachidonta variabilis

w.wµg/gverrucosaEriphiaw.wµg/g

Patella caerulea

w.wµg/g

0

5

10

15

20

25

30

35

40

45

(µg

/g.w

.wز(

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروسة C في الموقع Cu تركیز

20/6/2006 3/8/20065/10/2006 25/11/200620/1/2007 15/3/200722/5/2007 2/7/2007

(µg/g.w. w) Cu C

C

Monodonta Turbinata

w.wµg/g Patella caerulea

w.wµg/gBrachidonta variabilisEriphia verrucosa

Pinctada radiata

µg/g. w.w

0

1

2

3

4

5

6

7

8

(µg

/g.w

.wز (

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروسة C في الموق�ع Cr تركیز

20/6/2006 3/8/2006

5/10/2006 25/11/2006

20/1/2007 15/3/2007

22/5/2007 2/7/2007

µg/g.w. w) Cr C


٩٠

0

0.5

1

1.5

2

2.5

3

3.5

4

(µg

/g.w

.wز(

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروس�ة C في الموقع Pb تركیز

20/6/2006 3/8/20065/10/2006 25/11/200620/1/2007 15/3/200722/5/2007 2/7/2007

µg/g.w. w)Pb C


w.wµg/g verrucosaEriphia

Pinctada radiata Patella caeruleaw.wµg/g

Monodonta Turbinataw.wµg/g

0

0.2

0.4

0.6

0.8

1

1.2

(µg

/g.w

.wز(

كیتر

ال

Patella

verrucosa

Monodonta

Turbinata

Pinctada

radiata

Brachidonta

variabilus

Eriphia

verrucosa

األنواع المدروسة C في الموقع Cd تركیز

20/6/2006 3/8/20065/10/2006 25/11/200620/1/2007 15/3/200722/5/2007 2/7/2007

µg/g.w. w)Cd C

w.wµg/g

Monodonta Turbinata Pinctada radiata

Brachidonta variabilis Patella caerulea verrucosaEriphia

D

m



٩١

m

m

D

0

100

200

300

400

500

600

700

(µg

/g.w

.wز(

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروسة D في الموقع Zn تركیز

20/6/2006 3/8/20065/10/2006 25/11/200620/1/2007 15/3/200722/5/2007 2/7/2007

µg/g.w. w) ZnD

Pinctada radiata

w.wµg/gPatella caeruleaBrachidonta variabilis

Monodonta TurbinataverrucosaEriphia

Pinctada radiata

0

2

4

6

8

10

12

(µg

/g.w

.wز (

ك�یتر

ال

Patella

verrucosa

Monodonta

Turbinata

Pinctada

radiata

Brachidonta

variabilus

Eriphia

verrucosa

األنواع المدروسة D في الموقع Cu تركیز

20/6/2006 3/8/2006 5/10/200625/11/2006 20/1/2007 15/3/200722/5/2007 2/7/2007

µg/g.w. w)Cu D

D

w.wµg/g


٩٢


verrucosa Eriphia

EriphiaBrachidonta variabilis Monodonta Turbinata Patella caerulea

w.wµg/g

0

0.5

1

1.5

2

2.5

(µg

/g.w

.wز(

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروسة D في الموقع Cr تركیز

20/6/2006 3/8/2006 5/10/2006 25/11/200620/1/2007 15/3/2007 22/5/2007 2/7/2007

µg/g.w.w) Cr D

Brachidonta variabilis

w.wµg/g

w.wµg/g0.12

Patella caerulea verrucosaMonodonta Turbinata, EriphiaPinctada radiata

0

0.5

1

1.5

2

2.5

3(µ

g/g

.w.w

ز(كی

ترال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروسة D في الموقع Pb تركیز

20/6/2006 3/8/20065/10/2006 25/11/200620/1/2007 15/3/200722/5/2007 2/7/2007

µg/g.w. w)Pb D

Pinctada radiata

w.wµg/gPatella caerulea

w.wµg/g TurbinataMonodonta

µg/gBrachidonta variabilis

verrucosaEriphiaw.wµg/g



٩٣

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

(µg

/g.w

.wز(

كیتر

ال

Patellaverrucosa

MonodontaTurbinata

Pinctadaradiata


Eriphia verrucosa

األنواع المدروسة D في الموقع Cd تركیز

20/6/2006 3/8/20065/10/2006 25/11/200620/1/2007 15/3/200722/5/2007 2/7/2007

µg/g.w. w)Cd D

D



Patella caerulea Monodonta Turbinata verrucosaEriphia

µg/g0.87

Cd ,Pb ,Cr ,Cu ,Zn

Dw.wµg/gZn:Cu:4.92Cr: 1.07Pb: 1.30

Cw.wµg/gCu: 10.8, Zn:

132.55Cr: 2.21A

C

DA

0

20

40

60

80

100

120

140

(µg

/g.w

.wز(

كیتر

ال

ACD

مواقع الدراسة

Zn متوسط تركیز

Zn

Znµg/g.w. w)


٩٤

0

2

4

6

8

10

12

(µg/

g.w

.wز(

كیر ت

ال

ACD

مواقع الدراس�ة

Cd, Pb, Cr, Cu متوسط تراكیز العناصر

Cu

Cr

Pb

Cd

CuCrPbCd (µg/g.w. w)

ACD



٩٥

D, C, A

R= Patella caeruleaA

verrucosaEriphia

R=0.83ACD

Cd,Pb,Cr,Cu,Zn

Zn µg/g

Cu µg/g

Cr µg/g

Pb µg/g

Cd µg/g

- Augusta

Di Geronimo,1990

De Leon,1984

(Fishelson et al,1999)

Donax trunculus

Linnaeus ,1758

Usero et al, 2005)

Chamelea gallina

(Linnaeus,1758)

,1758

De Leon,1984)

- Augusta

Di Geronimo,1990


)Krauss,1962

Pinctada radiata

Leachi,1814


٩٦

Monodonta turbenata

Born,1780

Patella caerulea

Linnaeus ,1758

Eriphia verrucosa

Forskale,1775

Cd ,Pb ,Cr ,Cu ,Zn

ZnCuCr

PbCd

ZnCuCr

PbCd

pinctada radiate

ZnCuCrPbCd

pinctada radiata


Patella caeruleaTurbinata

MonodontaGastropoda



٩٧

C, Aw.wµg/g132.55

C

w.wµg/gCu: 10.8Cr: 2.21w.wµg/g

Dw.wµg/g

Patella caerulea A

R=0.94, n=8 D C

pinctada radiata

µg/g.w.w

µg/g.w.w

1. BELDI, H.GIMBERT, F.MAAS, S.SCHEIFLER, R.SOLTANI, N. "Seasonal variations Of Cd, Cu, Pb and Zn in the edible mollusc Donax trunculus (Mollusca, Bivalvia) From the gulf of Annaba" Algeria African. Journal of Agricultural Research. Vol. 1 (4), 2006.085-090.

2. DAVIS, N; BELL, D; GOLD, J. "Field Sampling Manual for the Regional Monitoring Program for Trace Substances" SanFrancisco Estuary Institute, Oakland, CA. 2001.60.

3. De LEON, A; R, MAS. "monitoring of heavy metals in super ficial Sediment and marine organisms Form the western Mediterranean coasi". VIes, journées étude pollution LucCrne, C.I.E.S.M. 1984.321-326.

4. DI GERONIMO, I. "Relation entre Bioco enoses et pollution dans la Baie D’AUGUSTA (SICILE ORIENTAIE)". UNEP. Athens No. 40. 1990.

5. DITTRITCH.K,MANDRY.R,WENNRICH.R. "Fortschritte in der Atomspektro- metrischen Spurenanalytik,Band 2 .VCH Verlagsgesellschaft mbH .Weinheim.Germany. 1986.

6. ISLAM MD, TANAKA M. " Impact of pollution on coastal and marine Ecosystems including coastal and marine fisheries and approach for Management: a review andsynthesis ". Mar. Pollut. Bull. 48 , 2004, 624-649.

7. FISHELSON, L; BRESLER, V; MANELISA, R; ZUK-RIMON, Z., DOTAN, A; HORNUNG H; YAWETZ, A. "Toxicological aspects associated with the ecology of Donax trunculus (Bivalvia, Mollusca) in a polluted environment". Sci. Total Environ. 226: 1999.121-131.

8. GAILLARD, J.M. " Mediterrian et Mer - Zone de pêche 37. Vol. 1. Fiches FAO D’Identification des espèces pour les besoins de la pêche". FAO & ECCE, Rome. 1978.

9. HANSON.N.W. "Official standardized and recommended methods of analysis 2nd, the Society for analysis chemistry, London. 1973.


٩٨

10. PARENZAN,P. " carta d’identita delle conehigilie del mediterraneo", vol .(II. Bivalvi Prima part ). Ed.Bios. taranto1974, 50.

11. PARENZAN,P. " carta d’identita delle conehigilie del mediterraneo". VOL .(II. Bivalvi P second ), Parte. 1976 ,1-225.

12. PIETRO, P. " Carta d’identita delle corchigile del Mediterraneo" . Vol. II .Bivalvi prima Parte . Ed . Bios – Taranto. 1974.

13. RIEDL , R . "Fauna and Flora des Mittelmeeres" . verlag paul parey Hamburg Berlin . 1983.1-832

14. UNEP (OCA) MED/G. "A regional site specific temporal Trend Monitoring Programme ".1997,. 9.

15. UNEP" Manual for the geochemical analysis of marine sediments and suspends Particulate matter ". Reference Methods for Marine Pollution Studies, No.63. 1995.

16. UNEP. " Sampling of selected marine organisms for sample preparation for Trace Metal analysis "Reference Methods for Marine Pollution Studies, UNEP/FAO/IOC/IAEA. N.7,Rev.2. 1984.15.

17. USERO J, MORILLO J, GRACIA I. " Heavy metal concentrations in Mollusks from the Atlantic coast of southern Spain". Chemosphere 59. 2005,1175-1181.

18. WATZIN, M.C; ROSCIGNO, P.R. " The effects of zinc contamination on the Recruitment and early survival of benthic invertebrates in an estuary "Marine Pollution Bulletin. Vol. 34, No.6, 1997.443-455.

19. WYSE, E.J; S.AZEMARD and S.J.de MORA, " Report on the world-wide Intercomparison exercise for the determination of trace elements and methyl mercury in fish homogenate IAEA-407 .IAEA/AL/144,IAEA/MEL/72,IAEA, 2003.94.

PbCdZn Cu

ZnCuCr PbCd Pinctada radiata Brachidonta variabilis

Cd,Pb,Cr,Cu,Zn


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