Daria Bedulina Laboratory of biosystem adaptation Irkutsk State University
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Transcript of Daria Bedulina Laboratory of biosystem adaptation Irkutsk State University
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Daria Bedulina
Laboratory of biosystem adaptation Irkutsk State University
Adviser: Dr M. Timofeev
Modification of MXR-activity in freshwater amphipods Eulimnogammarus cyaneus
and E. verrucosus by organic and inorganic contaminants.
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Organism
STRESSADAPTATION
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The organisms stress adaptation levels:
1. Behavioral2. Physiological3. Biochemical and molecular
Multixenobiotic resistance system (MXR)
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P-glycoprotein(P-gp)
Multixenobiotic resistance system (MXR)
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Method of MXR-activity definition:
Rhodamine B efflux method
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LAKE BAIKAL
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Lake Baikal
Amphipods (Crustacea, Amphipoda) More than 250 species and subspecies
Fauna >> 2500 species (60-80% endemics)
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Contaminants, used in research
Organic contaminant Natural organic matter (NOM)
Inorganic contaminant CdCl2
Humic substances
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The objects of research:
Eulimnogammarus verrucosus (Gerstf.)
E.cyaneus (Dyb.)
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P-gp
Rhodamine B-efflux method
Accumulation
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P-gp
Rhodamine B-efflux method
Efflux
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MXR-activity in amphipodsMXR-activity in amphipods
without contaminants (control)
(Rhodamine B efflux ))
Eulimnogammarus cyaneus Eulimnogammarus verrucosus
Results
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MXR-activity in amphipods, MXR-activity in amphipods,
NOM solutions (60 mg/ml)
(Rhodamine B efflux ))
Eulimnogammarus cyaneus Eulimnogammarus verrucosus
control
NOM
Results
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Eulimnogammarus cyaneus Eulimnogammarus verrucosus
control
CdCl2
Results
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Discussion
1. Baikalian amphipods use a MXR system for xenobiotics efflux.
2. Activity of this system is inhibited by some organic and inorganic contaminants.
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Conclusion
The multixenobiotic resistance mechanism in baikalian organisms is sensitive to organic and inorganic contaminants, such as heavy metal and NOM.
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This research was partially supported by grants of Russian Foundation for Basic Research
No. 05-04-97239-Baikal, 05-04-97263-Baikal
and 06-04-48099
The presented results was published
Timofeyev M., Shatilina Z.M., Bedulina D.S., Menzel, R., Steinberg C.E.W. Natural organic matter (NOM) has the potential to modify the multixenobiotic resistance (MXR) activity in freshwater amphipods Eulimnogammarus cyaneus (Dyb) and Eulimnogammarus verrucosus (Gerst.)// Comparative biochemistry and physiology, Part B 146 (2007) 496–503
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Thank you!