DAIRY TESTING · 2021. 1. 12. · Oxacillin (30) 4-8 Cefoperazone (50) 4-6 Dicloxacillin (30) 4-8...
Transcript of DAIRY TESTING · 2021. 1. 12. · Oxacillin (30) 4-8 Cefoperazone (50) 4-6 Dicloxacillin (30) 4-8...
DAIRY TESTING
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PerkinElmer’s AuroFlow™ Strip Tests detect the most commonly used antibiotics found in dairy cow’s milk. These tests are fast and easy-to-use in a laboratory or in the field and do not require any additional equipment. The AuroFlow™ Strip Tests are manufactured to test for European Union and CODEX maximum residue limits (MRL).
The AuroFlow™ PR1ME™ Strip Tests are the newest products released by PerkinElmer, in which a mixing step has been eliminated from the test procedure. You can now add milk directly to a sample tube, then insert the test strip immediately for results within minutes.
PerkinElmer also offers products for the detection of a multitude of drug residues, pasteurization verification, and Aflatoxin M1.
AM DISTRIBUTORS LOCATED IN AM EMEA DISTRIBUTORS LOCATED IN EMEA APAC DISTRIBUTORS LOCATED IN APAC
AUSTIN, TXBioo Scientific location
BRAZILIn Brazil, a rapidly increasing demand for dairy products is driven by a fast-growing middle class of more than 100 million people who are also demanding improvements in the quality of their food products.
CANADAIn Canada, it is illegal to give a cow artificial hormones to increase its milk production. This means no milk, cheese, yogurt, or other dairy products produced in Canada contain added hormones.
FINLANDThe top milk drinking country in the world is Finland, drinking about 95 gallons of milk per person per year.
GREECECenturies before bodybuilders & milk protein powder, ancient Greek Olympians used dairy products to fuel their performance. According to Greek historian Diogenes Laertius, the first Olympians ate a diet consisting of figs, cheese and wheat. The majority of the athletes’ protein came from cheese.
CHINAThe Chinese dairy industry has imported a quarter million dairy cows since 2009, sourcing cows from Australia and New Zealand, which produce more milk per cow than original Chinese breeds.
AUSTRALIAThe dairy industry first started in Australia with only two bulls and seven cows, which arrived with the First Fleet from Englandto New South Wales, in 1788.
RUSSIAAncient Russians used to put frogs in milk to prevent the milk from going sour. Russian Brown Frogs secrete antimicrobial peptides, which would prevent bacteria from growing and spoiling the milk.
DISTRIBUTORS CAN BE FOUND ALL OVER THE WORLD
Remove strip & visually interpret result using diagram or QuickSTARTM reader.
Incubate strip in the AuroBLOCKTM.03
05
04
AUROFLOWTM PR1METM
S T R I P T E S T P R O C E D U R E
02
01 Transfer 200 µL of raw milk to an empty sample tube in the AuroBLOCKTM.
Place test strip into sample tube.
Negative Positive
CBE
ORIENTATION
NegativeCeftiofurPositive
Beta-lactamPositive
TetracyclinePositive
CCEBE
TC = ControlCE = CeftiofurBE = Beta-lactamT = Tetracycline
BETA-LACTAM BT COMBO
The latest technology used in the AuroFlow™ PR1ME™ products removes the upfront mixing step, enabling the user to go from sample to results in less than 10 minutes with no work in between. The new PR1ME™ products have the capability of detecting 17 beta-lactams (including select metabolites) and 3 tetracyclines below EU MRL requirements. The faster, simplified protocol can be seen on the right.
AuroFlow™ PR1ME™
CATALOG # PRODUCT QTY
FOOD-1133-02 AuroFlow™ PR1ME™ Beta-lactam MRL Assay 100 strips
FOOD-1134-02 AuroFlow™ PR1ME™ BT Combo MRL Assay 100 strips
FOOD-6006-01 QuickSTAR™ Horizon™ Strip Reader 1 each
FOOD-6005 AuroBLOCK™ Incubator 1 each
New AuroFlow™ PR1ME™ Beta-lactam and BT Combo Kits
QuickSTAR™ Horizon™ Strip Reader
The QuickSTAR™ Horizon™ Strip Test Reader is a patented ruggedized handheld lateral flow testing system that provides rapid quantitative assay results and increases testing throughput and efficiency. The unit features a rechargeable lithium battery, a touchscreen menu driven interface, and provides test results in seconds. The unit is compatible with a wide array of pre-programmed test types and is software upgradeable to accommodate additional kits.
BETA-LACTAM ANTIBIOTICS
PENICILLINS CEPHALOSPORINS
Antibiotic (MRL)
Detection Limit (ppb)
Antibiotic (MRL)
Detection Limit (ppb)
Penicillin G (4) 1-2 Ceftiofur (100)* 10-30
Penethamate (4) 1-2 Desfuroylceftiofur (100)* 20-40
Ampicillin (4) 2-4 Cephapirin (60)* 4-7
Amoxicillin (4) 3-4 Desactyl cephapirin (60)* 12-24
Cloxacillin (30) 2-4 Cefazolin (50) 40-60
Oxacillin (30) 2-4 Cefoperazone (50) 3-5
Dicloxacillin (30) 1.5-3 Cephquinome (20) 8-12
Nafcillin (30) 4-8 Cephalonium (20) 1-2
Cefacetrile (125) 20-30
* MRL expressed as sum of parent and metabolite.
TETRACYCLINE ANTIBIOTICS
Antibiotic (MRL)
Detection Limit (ppb)
Tetracycline (100) 15-25
Chlortetracycline (100) 40-70
Oxytetracycline (100) 40-70
Doxycycline (NR) 35-55
BETA-LACTAM ANTIBIOTICS
PENICILLINS CEPHALOSPORINS
Antibiotic (MRL)
Detection Limit (ppb)
Antibiotic (MRL)
Detection Limit (ppb)
Penicillin G (4) 2-3 Ceftiofur (100) 85-95
Ampicillin (4) 3-4 Cephapirin (60) 4-8
Amoxicillin (4) 3-4 Desacetyl cephapirin (60) 20-30
Cloxacillin (30) 5-8 Cefazolin (50) 40-60
Oxacillin (30) 4-8 Cefoperazone (50) 4-6
Dicloxacillin (30) 4-8 Cephquinome (20) 8-10
Nafcillin (30) 10-15 Cephalonium (20) 2-4
Cefacetrile (125) 25-35
AuroFlow™ PR1ME™ Beta-lactam MRL Assay
• Rapid 3 minute, one-step method• Detects 14 beta-lactams below EU MRL requirements
AuroFlow™ PR1ME™ BT Combo MRL Assay
• Rapid 7 minute, one-step method• Detects 17 beta-lactams (including select metabolites) and 3 tetracyclines below
EU MRL requirements
AuroBLOCK™ Incubator
The AuroBLOCK™ Incubator is used to ensure all AuroFlow™ PR1ME™ kits receive a constant incubation temperature. A fixed temperature combined with optimized tubes from PerkinElmer enhances the flow of sample along the strip tests, allowing for proper visual interpretation. Each incubator arrives ready-to-use for all AuroFlow™ PR1ME™ kits with no programming needed, providing a user-friendly experience.
AuroFlow™
BETA-LACTAM ANTIBIOTICS
Substance (MRL)Detection Range (ppb)
1067-01 1087-01 1116-01
Penicillin G (4) 2-3 2-3 1-2
Ampicillin (4) 3-4 3-4 3-4
Amoxicillin (4) 3-4 3-4 3-4
Oxacillin (30) 4-8 4-8 2-5
Cloxacillin (30) 4-8 4-8 2-5
Dicloxacillin (30) 5-8 5-8 10-18
Nafcillin (30) 15-30 15-30 8-11
Cephquinome (20) 10-20 10-20 3-7
Ceftiofur (100) 75-100 75-100 36-75
Cefazolin (50) 35-50 35-50 20-45
Cephapirin (60) 6-15 6-15 5-8
Cephalonium (20) 4-8 4-8 4-10
Cefaperazone (50) 5-20 5-20 9-18
Cefacetrile (125) 30-50 30-50 21-28
TETRACYCLINE ANTIBIOTICS
Substance (MRL)Detection Range (ppb)
1067-01 1087-01 1116-01
Tetracycline (100) 50-100 50-100
Oxytetracycline (100) 50-70 21-32
Chlorotetracycline (100) 15-50 15-50
Doxycycline (0) 5-20 5-20
SULFONAMIDE ANTIBIOTICS
Substance (MRL)Detection Range (ppb)
1067-01 1087-01 1116-01
Sulfamethazine (100) 23-90
Sulfamethoxazole (100) 1-2
Sulfadiazine (100) 10-20
Sulfathiazole (100) 0.5-1
Sulfadimethoxine (100) 1-8
Sulfaquinoxaline (100) 18-32
Sulfapyridine (100) 5-10
Sulfamethoxypyridazine (100) 3-6
Sulfamerazine (100) 13-25
Sulfachloropyridazine (100) 0.5-2.5
Sulfamonomethoxine (100) 1-6
AUROFLOWTM
S T R I P T E S T P R O C E D U R E
4 min
Add dipstick to reaction well.
Visually interpret result using the diagram, or use reader.
Incubate mixture for 4 minutes at room temperature.
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3 min
Incubate mixture for 3 minutes at room temperature. 02
Add 200 µL cold raw milk to reaction well and mix by pipe�ing up and down 10 times.01
The AuroFlow™ lateral flow test strips are designed for the detection of a broad range of antibiotics in raw, commingled cow’s milk. These rapid tests detect 14 beta-lactam antibiotics, major tetracyclines, and 11 major sulfonamides in milk at or below EU and CODEX Maximum Residue Limits (MRL). The AuroFlow™ Combo kits allow simultaneous detection of different classes of antibiotic residues. Validation reports are available upon request showing the efficacy of the tests in both room temperature and cold milk. The fast, simplified protocol can be seen below.
CATALOG # PRODUCT QTY
FOOD-1067-01 AuroFlow™ Beta-Lactam Strip Test Kit 96 strips
FOOD-1087-01 AuroFlow™ BT Combo Strip Test Kit 96 strips
FOOD-1116-01 AuroFlow™ BTS Combo Strip Test Kit 96 strips
Easy-to-Use Strip Tests for the Rapid Screening of Antibiotic Residues in Milk
• Easy-to-use strip test with no required heating step or incubator• Works with room temperature and cold milk• Results in only 7 minutes• Results easy to interpret visually or by use of QuickSTAR™ Horizon™ Strip Reader
OTHER
CATALOG#FOOD-6003-01
MaxSignal® 4302 Microplate Reader
The MaxSignal® 4302 Microplate Reader is a versatile microplate spectrophotometer for 96-well plates providing all the functionality needed for numerous laboratory applications while offering great performance and value.
The MaxSignal® 4302 Microplate Reader is a compact, PC-controlled, multi-purpose instrument designed to read and calculate the results of microplate-based assays. Plates containing microstrips can be read as whole plates or as partial strips. The compact space saving design makes it ideal for stand-alone use.
• Sample extraction protocols for both milk and yogurt
• Milk protocol does not require sample prep• High recovery rates• Ready to use standards
• Rapid and simple method• Minimal sample preparation• Highly accurate and reproducible • Visual results; no reader necessary
CATALOG # PRODUCT QTY
FOOD-1060-05 MaxSignal® Aflatoxin M1 ELISA Kit 96 tests
CATALOG # PRODUCT QTY
FOOD-1041 MaxSignal® Dairy Product Pasteurization Verification Kit30
samples
Test for Aflatoxin M1 Residues in Milk and Yogurt
Rapid Assay for Pasteurization Confirmation of Milk, Cheese, and Other Dairy Products
ANTIBIOTIC
MaxSignal® Amoxicillin ELISA KitMaxSignal® Beta-Lactam ELISA KitMaxSignal® Chloramphenicol ELISA KitMaxSignal® Chlortetracycline ELISA KitMaxSignal® Ciprofloxacin ELISA KitMaxSignal® Doxycycline ELISA KitMaxSignal® Fluoroquinolone ELISA KitMaxSignal® Lincomycin ELISA KitMaxSignal® Oxytetracycline ELISA KitMaxSignal® Sarafloxacin ELISA KitMaxSignal® Sulfamethazine ELISA KitMaxSignal® Sulfamethoxazole ELISA KitMaxSignal® Sulfonamide ELISA KitMaxSignal® Tetracycline ELISA Kit
OTHER RESIDUES & TESTS
MaxSignal® Diethylstilbestrol ELISA KitMaxSignal® Melamine ELISA Kit
OTHER DAIRY TEST KITS
PerkinElmer, Inc.7050 Burleson RdAustin, TX 78744 USAP: (888) 208-2246 or(+1) 512-707-8993www.biooscientific.com
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BR50-1000-01 • Rev. C