Cytology
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Transcript of Cytology
CytologyBY: MOSTAFA SAFIAN
نسألكم الدعاء
بسم الله الرحمن الرحيم
Mitochondria
-stain :
iron haematoxlin
-structure :black granules or filaments forming the basal striations in the striated duct -occurrence :in salivary glands- liver- kidney- muscle
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Mitochondria
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Golgi apparatus
-stain : silver impregnation
-structure :browen or black fibillar,
round or oval structures
-occurrence :in the apical cytoplasm
of the epithelium lining the epididymis
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Myofibrils
-stain : 1-Haematoxylin&Eosin
2-Silver impregnation
-structure :-in skeletal muscle fiber:
-in longitudinal section, the myofibrilsform light and dark bands
-in cross section, the myofibrils appearas fine red points in a polyhedral shaped muscle fiber
-occurrence :skeletal muscle fiber
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Myofibrils
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Myofibrils
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Myofibrils
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Proteins(zymogen granules)
-stain :-Haematoxylin&Eosin
-structure :Acidophilic(red) substance
filling the apical cytoplasm of the pancreatic acinar cells
representing the zymogen granules
-occurrence :In pancreatic acinar cells
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Melanin pigment
-structure :Melanin granules
( black to brown) scatter in the iris
or ciliary processes of the eye
and in the basal layer of skin
-occurrence :Ciliary process of eye or skin
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Melanin pigment
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Goblet cell(carbohydrate)
-stain :
Periodic acid Schiff(PAS)
-structure :goblet cell :
exocrine unicellular gland consist of two parts
1-apical expended portion appear emptydue to dissolved carbohydrates
2 -narrow slender distal portion contain compressed nucleus
-occurrence :Small intestine
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Goblet cell(carbohydrate)
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Goblet cell(carbohydrate)
Stain:(PAS)
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Goblet cell(carbohydrate)
Stain:(PAS)
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Goblet cell(carbohydrate)
Stain:(PAS)
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Goblet cell(carbohydrate)
1-Eosin & hematoxylin
2-Stain:(PAS)
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Lipid
-stain :
1-Hx & Eosin2-Osmic acid or Suddan black
-structure :1- By Hx & Eosin the fat cells appear
as clear empty space surrounded by thin film of red cytoplasm
containing flat basophilic nucleus
2 -By osmic acidor suddan black the flat cells appear as large sherical or round homogenous black spots
-occurrence :Perineural fat
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Lipid
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Lipid
Stain: Sudan black
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Lipid
Stain: Sudan black
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Lipid
Stain: Sudan black
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Simple squamousepithelium
-structure :Appear as single layer of flat cells.
their cytoplasm contains flat nucleus
located in the wide central part
-occurrence :Parietal layer of
bowman's capsule
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Simple squamousepithelium
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Simple squamousepithelium
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Simple squamousepithelium
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Simple cuboidal
-structure :Single layer of cubic shaped cells
(height and width nearly equal .)the cells appear as squares, each
cell contains round central nucleus
-occurrence :Thyroid follicle
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Simple cuboidal
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Simple cuboidal
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Simple columnarepithelium
-structure :single layer of epithelial cells.
the cells appear tall and narrow
( their height is greater than their width )
their nuclei are oval and located in the basal half of the cell
-occurrence :In large intestine
and stomach
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Pseudostratified columnar epithelium
-structure :it's so, called because
the nuclei are seen at different levels
but all the cells rest on the basement membrane
and not all the cells reachto the surfaceconsist of:
1-tall cell, with proximally located nucleus
2-short cell, with distally located nucleus
-occurrence :Epididymis
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Pseudostratified columnar epithelium
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Pseudostratified columnar epithelium
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Pseudostratified columnar epithelium
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Pseudostratified columnar epithelium
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transitional epithelium
-structure :1-basal cell layer:
one layer of cuboidal cells2-middle layer :
several layer of polyhedral cells toward the base
and bear shaped with their bases
toward the surface3-superfescial layer :
cuboidalcells with convex surface
(umbrella -dome shaped cells)with (1-2) nuclei
-occurrence :Urinary bladder,
ureter, urethra
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transitional epithelium
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transitional epithelium
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transitional epithelium
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transitional epithelium
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stratified squamous epithelium
-structure :1-basal layer :
one layer of columnar or cuboidal 2-middle layer:
several layers of irregular polyhedral cells with
rounded nuclei3-superfescial layer :
squamous
-occurrence :A-cornified: skinB- non-cornified: oesophagus
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non-cornified
stratified squamous epithelium cornified
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non-cornified
stratified squamous epithelium cornified
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non-cornified
stratified squamous epithelium cornified
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non-cornified
stratified squamous epithelium cornified
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non-cornified
stratified squamouscornified
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cornified
stratified squamouscornified
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cornified
Mucous gland
-occurrence:
palatine salivary glands oesophageal glands of dogs
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Mucous gland
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Serous gland
-occurrence:
pancreas, parotid salivary glands
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Mixed gland
-occurrence:
mandibular salivary glands
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Apocrine gland
-occurrence:
Mammary gland
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Apocrine gland
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Apocrine gland
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Apocrine gland
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Apocrine gland
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Apocrine gland
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Holocrine gland
-occurrence:
sabaceous gland
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Holocrine gland
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Holocrine gland
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Holocrine gland
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Holocrine gland
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