Cytokine assays portfolio - cisbio.com · Cisbio’s kits cover the full range of cytokines:...

Life Sciences

Cytokine assays

Cisbio’s kits cover the full range of cytokines: interleukins, interferons (IFN), chemokines, TNF and lymphokines.

All our assays are cell based and measure cytokine release in cell supernatant.

They have been validated in several cell lines, primary cells and PBMCs to ensure relevance, and their low sample requirement enables the quantification of multiple cytokines in parallel from the same supernatant.

The HTRF® protocol makes them a fast alternative to ELISA or other measurement methods for researchers in the fields of oncology, inflammatory diseases or any other inflammation process.

This leaflet presents the analytical performances of each assay, the associated standard curve* and the list of available references**.

* To learn more about data processing and 4PL 1/y² fitting, we invite you to visit our dedicated webpage at http://www.cisbio.com/4PL-regression

** Bulk size kits are available - please contact us

A full range of cytokines

STRONG ASSAYS RELY ON VALIDATION

Parallel measurementsFrom start to finish

Research

Screening

Supernatant from:

Lyophilized standards

Low sample volume: 16 µL

Same buffers

Single protocol

4PL 1/y² fitting

One technology

Fresh blood

PBMCs

B and T cells

Immortalized cell lines

Human G-CSF kit p.6

Human GM-CSF kit (CSF2) p.7

Human IFNa kit p.8

Human IFNb kit p.9

Human IFNγ kit p.10

Mouse IFNγ kit p.11

Human IL1a kit p.12

Mouse IL1a kit p.13

Human IL1b kit p.14

Mouse IL1b kit p.15

Human IL2 kit p.16

Mouse IL2 kit p.17

Human IL4 kit p.18

Human IL5 kit p.19

Human IL6 kit p.20

Mouse IL6 kit p.21

Human IL8 kit p.22

Human IL10 kit p.23

Human IL12 kit p.24

Human IL13 kit p.25

Human IL17 kit p.26

Mouse IL17 kit p.27

Human IL18 kit p.28

Human IL22 kit p.29

Human IL23 kit p.30

Human IL31 kit p.31

Human & Mouse TGFb1 kit p.32

Human TNFa kit p.33

Mouse TNFa kit p.34

Cynomolgus TNFa kit p.35

Human CCL2 kit (MCP1) p.36

Mouse CCL2 kit (MCP1) p.37

Human CCL3 kit (MIP1a) p.38

Human CCL4 kit (MIP1b) p.39

Human CCL5 kit (RANTES) p.40

Human CCL20 kit p.41

Human CXCL1 kit (GROa) p.42

Human CXCL10 kit (IP10) p.43

6

Human G-CSF kit

Ordering information:

0 1000 2000 3000 4000 50000

2000

4000

6000

8000

[hG-CSF] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI

Assay range 47 to 4,500 pg/mL

LOD 27 pg/mL 27 pg/mL 27 pg/mL

LOQ 47 pg/mL - -

Incubation time Overnight at room temperature

Conversion factor NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF value (pg/mL)

Product Size Part#

Human G-CSF kit 500 tests 62HGCSFPEG

Human G-CSF kit 10,000 tests 62HGCSFPEH

7

Human GM-CSF kit (CSF2)

Diluent DMEM RPMI



LOQ 54 pg/mL - -

Incubation time 3h at room temperature

Conversion factor NIBSC (88/646) value (IU/mL) = 0,01 x HTRF hGM-CSF value (pg/mL)


Product Size Part#

Human GM-CSF kit 500 tests 62HGMCSFPEG

Human GM-CSF kit 10,000 tests 62HGMCSFPEH

[hGM-CSF] (pg/mL)

Delta

Rat

io

0 2000 4000 6000 8000 100000

2000

4000

6000

8000

8

Human IFNa kit


Diluent DMEM RPMI

Assay range 36 to 4000 pg/mL


LOQ 36 pg/mL - -


Conversion factor NIBSC (94/784) value (IU/mL) = 0,9 x HTRF hIFNa value (pg/mL)

Product Size Part#

Human IFNa kit 500 tests 62HIFNAPEG

Human IFNa kit 10,000 tests 62HIFNAPEH

0 1000 2000 3000 4000 50000

5000

10000

15000

[hIFNα] (pg/ml)

Delta

Rat

io

9

Human IFNb kit


Diluent DMEM RPMI



LOQ 19 pg/mL - -


Conversion factor NIBSC (00/572) value (IU/mL) = 0,44 x HTRF hIFNb value (pg/mL)

Product Size Part#

Human IFNb kit 500 tests 62HIFNBPEG

Human IFNb kit 10,000 tests 62HIFNBPEH

Delta

Rat

io

0

2000

4000

6000

8000

10000

12000

0 1000 2000 3000 4000[hIFNβ] (pg/mL)

10

Human IFNγ kit


0 1000 2000 3000 4000 50000

2500

5000

7500

10000

[hIFNγ] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 21 pg/mL - -


Conversion factor NIBSC (82/587) value (IU/mL) = 0,019 x HTRF hIFNγ value (pg/mL)

Product Size Part#

Human IFNγ kit 1 x 96 tests 62HIFNGPET

Human IFNγ kit 500 tests 62HIFNGPEG

Human IFNγ kit 10,000 tests 62HIFNGPEH

11

Mouse IFNγ kit


Diluent DMEM RPMI



LOQ 34 pg/mL - -


Product Size Part#

Mouse IFNγ kit 500 tests 62MIFNGPEG

Mouse IFNγ kit 10,000 tests 62MIFNGPEH

0 1000 2000 3000 40000

2000

4000

6000

8000

[mIFNγ] (pg/ml)

Delta

Rat

io

12

Human IL1a kit


0 2000 4000 60000

5000

10000

15000

20000

25000

[hIL1α ] (pg/ml)

Delta

Rat

io

Diluent DMEM RPMI

Assay range 20.5 to 5,000 pg/mL


LOQ 20.5 pg/mL - -


Conversion factor NIBSC (86/632) value (IU/mL) = 10 x HTRF hIL1a value (pg/mL)

Product Size Part#

Human IL1a kit 500 tests 62HIL1APEG

Human IL1a kit 10,000 tests 62HIL1APEH

13

Mouse IL1a kit


Diluent DMEM RPMI



LOQ 62 pg/mL - -


Conversion factor NIBSC (93/672) value (IU/mL) = 1,2 x HTRF mIL1a value (pg/mL)

Product Size Part#

Mouse IL1a kit 500 tests 62MIL1APEG

Mouse IL1a kit 10,000 tests 62MIL1APEH

0 1000 2000 3000 4000 50000

2000

4000

6000

[mIL1α ] (pg/ml)

Delta

Rat

io

14

Human IL1b kit


0 2000 4000 6000 80000

10000

20000

30000

[hIL1β ] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 39 pg/mL - -


Conversion factor NIBSC (86/680) value (IU/mL) = 0,1 x HTRF hIL1b value (pg/mL)

Product Size Part#

Human IL1b kit 1 x 96 tests 62HIL1BPET

Human IL1b kit 500 tests 62HIL1BPEG

Human IL1b kit 10,000 tests 62HIL1BPEH

15

Mouse IL1b kit


Diluent DMEM RPMI



LOQ 46 pg/mL - -


Conversion factor NIBSC (93/668) value (IU/mL) = 0,1 x HTRF mIL1b value (pg/mL)

Product Size Part#

Mouse IL1b kit 500 tests 62MIL1BPEG

Mouse IL1b kit 10,000 tests 62MIL1BPEH

0 1000 2000 3000 4000 50000

1000

2000

3000

4000

5000

[mIL1β ] (pg/ml)

Delta

Rat

io

16

Human IL2 kit


0 2000 4000 6000 8000 100000

5000

10000

15000

[hIL2] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 37 pg/mL - -


Conversion factor NIBSC (86/500) value (IU/mL) = 0,01 x HTRF hIL2 value (pg/mL)

Product Size Part#

Human IL2 kit 1 x 96 tests 62HIL02PET

Human IL2 kit 500 tests 62HIL02PEG

Human IL2 kit 10,000 tests 62HIL02PEH

17

Mouse IL2 kit


Diluent DMEM RPMI



LOQ 35 pg/mL - -


Product Size Part#

Mouse IL2 kit 500 tests 62MIL02PEG

Mouse IL2 kit 10,000 tests 62MIL02PEH

0 1000 2000 30000

5000

10000

15000

20000

[mIL2] (pg/ml)

Delta

Rat

io

18

Human IL4 kit

0 1000 2000 3000 4000 50000

5000

10000

15000

[hIL4] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 29 pg/mL - -




Product Size Part#



19

Human IL5 kit


Product Size Part#



0 5000 10000 150000

5000

10000

15000

[hIL5] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 71 pg/mL - -



20

Human IL6 kit

0 2000 4000 6000 80000

5000

10000

15000

20000

[hIL6] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 34 pg/mL - -




Product Size Part#




21

Mouse IL6 kit


Product Size Part#



0 2000 4000 6000 80000

10000

20000

30000

40000

50000

[mIL6] (pg/ml)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 19 pg/mL - -


Conversion factor NIBSC (93/730) value (IU/mL) = 0,7 x HTRF mIL6 value (pg/mL)

22

Human IL8 kit

0 1000 2000 3000 4000 50000

5000

10000

15000

[hIL8] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 32 pg/mL - -




Product Size Part#




23

Human IL10 kit


Product Size Part#



0 2000 4000 6000 80000

10000

20000

30000

40000

50000

[hIL10] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 22 pg/mL - -



24

Human IL12 kit

0 5000 10000 150000

10000

20000

30000

40000

[hIL12] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 81 pg/mL - -




Product Size Part#



25

Human IL13 kit

Diluent DMEM RPMI



LOQ 17 pg/mL 24 pg/mL 25 pg/mL


Conversion factor NIBSC (94/622) value (IU/mL) = 1.2.10-3 x HTRF hIL13 value (pg/mL)


Product Size Part#



[hIL13] (pg/mL)

Delta

Rat

io

0 1000 2000 3000 4000 50000

5000

10000

15000

20000

26

Human IL17 kit

0 5000 10000 150000

5000

10000

15000

[hIL17] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 33 pg/mL - -




Product Size Part#




27

Mouse IL17 kit

0 1000 2000 3000 4000 50000

10000

20000

30000

[mIL17] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 16 pg/mL - -



Product Size Part#



28

Human IL18 kit


Product Size Part#



0 2000 4000 6000 8000 100000

5000

10000

15000

20000

[hIL18] (pg/ml)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 43 pg/mL - -


Conversion factor NIBSC (03/200) value (IU/mL) = 5 x HTRF hIL18 value (pg/mL)

29

Human IL22 kit


Product Size Part#



Diluent DMEM RPMI




Incubation time Overnight at room temperature (reading possible from 3h)

Conversion factor No International standard available

[hIL22] (pg/mL)

Delta

Rat

io

0 1000 2000 3000 4000 50000

5000

10000

15000

20000

30

Human IL23 kit

0 10000 20000 30000 400000

10000

20000

30000

40000

[hIL23] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 92 pg/mL - -

Incubation time 3h at 4°C


Product Size Part#



31

Human IL31 kit


Product Size Part#



0 5000 10000 15000 20000 250000

5000

10000

15000

[hIL31] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 87 pg/mL - -


32

Human & Mouse TGFb1 kit


Product Size Part#

TGFb1 kit 500 tests 62HTGFBPEG

TGFb1 kit 10,000 tests 62HTGFBPEH

Diluent DMEM + 4% FCS

RPMI + 4% FCS





Conversion factor NIBSC (89/514) value (U/mL) = 0,017 x HTRF hTGFb1 value (pg/mL)

[hTGFβ1] (pg/mL)

Delta

Rat

io

0 500 1000 1500 20000

2000

4000

6000

33

Human TNFa kit

0 1000 2000 30000

2000

4000

6000

[hTNFα ] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 29 pg/mL - -


Conversion factor NIBSC (12/154) value (IU/mL) = 0,1 x HTRF hTNFa value (pg/mL)


Product Size Part#

Human TNFa kit 1 x 96 tests 62HTNFAPET

Human TNFa kit 500 tests 62HTNFAPEG

Human TNFa kit 10,000 tests 62HTNFAPEH

34

Mouse TNFa kit


Product Size Part#

Mouse TNFa kit 500 tests 62MTNFAPEG

Mouse TNFa kit 10,000 tests 62MTNFAPEH

0 2000 4000 6000 80000

5000

10000

15000

20000

[mTNFα ] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 20 pg/mL - -


35

Cynomolgus TNFa kit

0 1000 2000 3000 40000

5000

10000

15000

[cTNFα ] (pg/ml)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 27 pg/mL - -



Product Size Part#

Cynomolgus TNFa kit 500 tests 62CTNFAPEG

Cynomolgus TNFa kit 10,000 tests 62CTNFAPEH

36

Human CCL2 kit (MCP1)


Product Size Part#

Human CCL2 kit (MCP1) 500 tests 62HCCL2PEG

Human CCL2 kit (MCP1) 10,000 tests 62HCCL2PEH

0 1000 2000 3000 40000

1000

2000

3000

4000

5000

[hCCL2] (pg/ml)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 90 pg/mL - -


Conversion factor NIBSC (92/794) value (IU/mL) = 0,9 x HTRF hCCL2 value (pg/mL)

37

Mouse CCL2 kit (MCP1)


Product Size Part#

Mouse CCL2 kit 500 tests 62MCCL2PEG

Mouse CCL2 kit 10,000 tests 62MCCL2PEH

Diluent DMEM RPMI



LOQ 13 pg/mL - -


[mCCL2] (pg/mL)

Delta

Rat

io

0 500 1000 1500 20000

10000

20000

30000

38

Human CCL3 kit (MIP1a)


Product Size Part#

Human CCL3 kit 500 tests 62HCCL3PEG

Human CCL3 kit 10,000 tests 62HCCL3PEH

Diluent DMEM RPMI






[hCCL3] (pg/mL)

Delta

Rat

io

0 880 1760 2640 3520 44000

2000

4000

6000

8000

10000

12000

39

Human CCL4 kit (MIP1b)

0 2000 4000 60000

10000

20000

30000

40000

[hCCL4] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 20 pg/mL - -



Product Size Part#

Human CCL4 kit (MIP1b) 500 tests 62HCCL4PEG

Human CCL4 kit (MIP1b) 10,000 tests 62HCCL4PEH

40

Human CCL5 kit (RANTES)


Product Size Part#

Human CCL5 kit 500 tests 62HCCL5PEG

Human CCL5 kit 10,000 tests 62HCCL5PEH

Diluent DMEM RPMI



LOQ 18 pg/mL - -



[hCCL5] (pg/mL)

Delta

Rat

io

0 500 1000 1500 2000 25000

2000

4000

6000

8000

10000

41

Human CCL20 kit


Product Size Part#

Human CCL20 kit 500 tests 62HCC20PEG

Human CCL20 kit 10,000 tests 62HCC20PEH

0 500 1000 1500 2000 25000

2000

4000

6000

[hCCL20] (pg/ml)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 47 pg/mL - -


42

Human CXCL1 kit (GROa)

0 1000 2000 3000 4000 50000

1250

2500

3750

5000

[hCXCL1] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI



LOQ 36 pg/mL - -

Incubation time 2h at 4°C


Product Size Part#

Human CXCL1 kit (GROa) 500 tests 62HCXC1PEG

Human CXCL1 kit (GROa) 10,000 tests 62HCXC1PEH

43

Human CXCL10 kit (IP10)


Product Size Part#

Human CXCL10 kit (IP10) 500 tests 62HCX10PEG

Human CXCL10 kit (IP10) 10,000 tests 62HCX10PEH

0 2000 4000 60000

5000

15000

20000

10000

25000

[hCXCL10] (pg/mL)

Delta

Rat

io

Diluent DMEM RPMI


LOD 1.8 pg/mL 3.8 pg/mL 4.2 pg/mL

LOQ 7 pg/mL - -


MORE THAN PRODUCTS, KNOWLEDGE!

www.cisbio.com/cytokines

Find helpful reference material, content, and expert advice by visiting our website at www.cisbio.com/cytokines

http://www.cisbio.com/gpcr

VIDEOS

APPLICATION NOTES

POSTER

How to quantify secreted cytokines using no-wash, add-and-read HTRF assays

Guidelines from PBMC isolation to cytokine assay optimisation

Cytokine secretion profile on PBMCs

HTRF® measurement of cytokine release from fresh blood samples

How to combine Cisbio assays to measure several cytokines from the same supernatant

Human G-CSF kit p.6

Human GM-CSF kit (CSF2) p.7

Human IFNa kit p.8

Human IFNb kit p.9

Human IFNγ kit p.10

Human IL1a kit p.12

Human IL1b kit p.14

Human IL2 kit p.16

Human IL4 kit p.18

Human IL5 kit p.19

Human IL6 kit p.20

Human IL8 kit p.22

Human IL10 kit p.23

Human IL12 kit p.24

Human IL13 kit p.25

Human IL17 kit p.26

Human IL18 kit p.28

Human IL22 kit p.29

Human IL23 kit p.30

Human IL31 kit p.31


Human TNFa kit p.33

Human CCL2 kit (MCP1) p.36

Human CCL3 kit (MIP1a) p.38

Human CCL4 kit (MIP1b) p.39

Human CCL5 kit (RANTES) p.40

Human CCL20 kit p.41

Human CXCL1 kit (GROa) p.42

Human CXCL10 kit (IP10) p.43

Mouse IFNγ kit p.11

Mouse IL1a kit p.13

Mouse IL1b kit p.15

Mouse IL2 kit p.17

Mouse IL6 kit p.21

Mouse IL17 kit p.27


Mouse TNFa kit p.34

Mouse CCL2 kit (MCP1) p.37

Cynomolgus TNFa kit p.35

Available assays listed by species

MDG010 - 04/2019 - Copyright Cisbio Bioassays - All trademarks are the property of Cisbio Bioassays.

Europe and other countries +33(0)466 796 705 | [email protected]

U.S. and Canada 1-888-963-4567 | [email protected]

China +86 21 5018 9880 | [email protected]

Japan +81-(0)43-306-8712 | [email protected]

www.cisbio.com

Cytokine assays portfolio - cisbio.com · Cisbio’s kits cover the full range of cytokines:...

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Transcript of Cytokine assays portfolio - cisbio.com · Cisbio’s kits cover the full range of cytokines:...