Cystic Fibrosis From Gene to Protein

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Cystic Fibrosis From Gene to Protein Class instructions

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Cystic Fibrosis From Gene to Protein. Class instructions. In this lesson. Look at the normal and mutated CFTR gene Make messenger RNA (mRNA) from DNA- transcription Make protein from mRNA- translation. The devil is in the detail!. - PowerPoint PPT Presentation

Transcript of Cystic Fibrosis From Gene to Protein

Page 1: Cystic Fibrosis From Gene to Protein

Cystic FibrosisFrom Gene to

ProteinClass instructions

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In this lesson

• Look at the normal and mutated CFTR gene

• Make messenger RNA (mRNA) from DNA- transcription

• Make protein from mRNA- translation

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The devil is in the detail!

• The 5’ prime and 3’ prime ends of the bases must be round the right way!

IMPORTANT: Do not take

bases apart!!!

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Correct base pairing is critical!

• Green (Guanine) pairs with yellow (Cytosine)

• Blue (Adenine) pairs with orange (Thymine)

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Lesson – part 1Normal CFTR Gene ΔF508 CF Mutation

Sense

Antisense

Sense

Antisense

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Transcription – Step 1

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Base pairing in mRNA- use brown sugars

• Green (Guanine) pairs with yellow (Cytosine)

• Blue (Adenine) pairs with light blue (Uracil)

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Transcription – Step 2• To make an mRNA, you must use the

antisense (red sugars) strand as as the template as base pairing is complimentary.

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Resulting mRNA

Normal CFTR mRNA ΔF508 CFTR mRNA

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Translation

• Turns mRNA into protein• Use transfer RNA (tRNA) which are

attached to amino acids as intermediate

• Amino acids have a direction too!

N-terminal C-terminal

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Transfer RNA

UAAIsoleucine

CAAValine

CCAGlycine

AAAPhenylalanine

UAAIsoleucine

tRNA – holds an anticodon at one end, and an amino acid at the other

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Using mRNA in 5’-3’ orientation, translate into

protein.

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Separate tRNA & protein from mRNA

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Resulting protein

Isoleucine – Phenylalanine - Glycine

Isoleucine – Glycine - Valine

Normal CFTR protein

ΔF508 CFTR protein

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Polymerase Chain Reaction

• Using the original chromosomes, separate strands

• Find where primers stick to each strand

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Genetic Test • Using the original chromosomes, see what a PCR

product will look like.• Remember: Each individual will have 2 chromosomes

- one inherited from mother and one from father.

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DNA gel genetic test result


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