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Cyclooxygenase-2 OverexpressionIs Related to Polypoid Growth andK-ras Gene Mutation in T1Colorectal CarcinomasTakuya Okawa, M.D.,1 Keigo Yoshinaga, M.D.,2 Hiroyuki Uetake, M.D.,1

Takanobu Sato, M.D.,1 Tetsuro Higuchi, M.D.,1 Tetsuo Nemoto, M.D.,3

Kenichi Sugihara, M.D.1

1 Department of Digestive Surgery, Tokyo Medical and Dental University Graduate School, Tokyo, Japan2Department of Surgery, National Printing Bureau Tokyo Hospital, Tokyo, Japan3 Department of Human Pathology, Tokyo Medical and Dental University Graduate School, Tokyo, Japan

PURPOSE: Cyclooxygenase-2 is thought to play a role in thedevelopment of intestinal tumors and levels are elevated inapproximately 80 to 90 percent of human colorectal carci-nomas. To clarify the role that cyclooxygenase-2 plays in thedevelopment of colorectal carcinoma, we studied the rela-tionship between cyclooxygenase-2 expression and tumormorphology and that between cyclooxygenase-2 expres-

sion and K-ras mutation. METHODS: We classified 48 T1colorectal carcinomas as polypoid or nonpolypoid and ana-lyzed the clinicopathologic features. The expression of cy-clooxygenase-2 was determined immunohistochemically,and nested polymerase chain reaction-restriction fragmentlength polymorphism detected a K-rascodon 12 mutation.RESULTS: Cyclooxygenase-2 expression was higher in pol-

ypoid carcinomas than in nonpolypoid carcinomas (P 0.999Adenomatous component 19 (63.3%) 0 (0%)

11 (36.7%) 18 (100%)

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Medicine, for advice regarding the molecular biology

techniques.

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