CV's_BCIL
14
CURRICULUM VITAE NAME V.Venkata Suryanarayana DATE AND PLACE OF BIRTH 31 st August 1975 Visakhapatnam, Andhra pradesh India NATIONALITY Indian SEX Male MARITAL STATUS Single PERMANENT ADDRESS 39-23-28/1, Vamsi nagar Madhavadhara, Visakhapatnam Andhrapradesh 530007 India Ph: 00-91-9440481554 ADDRESS FOR CORRESPONDENCE Plot No-64, H.No- 1-19-164/5, Venkata Sai Nagar Colony West Venkatapuram Trimulghery post Secunderabad-500015 India Email: [email protected] ,
description
cv
Transcript of CV's_BCIL
CURRICULUM VITAE
CURRICULUM VITAE
NAME
V.Venkata Suryanarayana
DATE AND PLACE OF BIRTH31st August 1975
Visakhapatnam, Andhra pradesh
India
NATIONALITYIndian
SEXMale
MARITAL STATUSSingle
PERMANENT ADDRESS
39-23-28/1, Vamsi nagar
Madhavadhara, Visakhapatnam
Andhrapradesh 530007
India
Ph: 00-91-9440481554
ADDRESS FOR CORRESPONDENCEPlot No-64, H.No- 1-19-164/5,
Venkata Sai Nagar Colony
West Venkatapuram
Trimulghery post
Secunderabad-500015
India
Email: [email protected],
Mobile: +91-9492925781
ACADEMIC QUALIFICATIONS
DegreeYear Subjects studiedUniversity/CollegeDivision/Percentage
Ph.D2001-2008Centre for Cellular and Molecular Biology, Hyderabad
M.Tech (Biotechnology)1997-99Biochemical Engg
Bioprocess Modelling & simulation
Enzyme Engineering
Rec. DNA Tech
Bioinformatics
Biochemistry
Immunology
Anna University, ChennaiFirst/ 9.05
(CGPA out of 10)
M.Sc
(Biochemistry)
1995-97Bioorganic Chemistry
Metabolism
Enzymology
Molecular Biology
Genetics
Clinical Biochemistry
Immunology
Cell BiologyAndhra University, Visakha PatnamFirst/ 70
B.Sc1992-95Mathematics
Biochemistry
ChemistryAndhra University, Visakha PatnamFirst/ 75
RESEARCH EXPERIENCE1.Ph.D. CCMB, India. Doctoral thesis Molecular status of Detoxification genes and functional proteomics in recurrent early pregnancy loss (REPL)
Supervisor: Dr. Lalji Singh, Director, Centre For Cellular and Molecular Biology, Hyderabad.
Description: REPL is a heterogeneous condition experienced by about 20% couples and in half of the cases the reasons are unknown. Present study is aimed at dissecting out the molecular genetic markers responsible for REPL in South Indian women. Mutational screening of genes involved in detoxification, estrogen metabolism, blood homeostasis, vascular activity etc provided us with exciting results with a strong association between the risk of recurrent miscarriages and the polymorphic markers studied was found. In addition, a number of novel polymorphisms that were not reported earlier had been discovered. This data is valuable for appropriate model development to assess the risk associated with a particular or combination of alleles.
Another part of the study is targeted at the analysis of blood platelet proteome and a comparison between normal and REPL females to fish out the candidate proteins that can be used as diagnostic markers. Platelet proteome showed consistent differences between cases & controls and the identification of the proteins by MALDI-TOF and peptide mass fingerprinting was done.
Techniques Used: DNA isolation from blood, PCR primer design, synthesis and amplification of DNA, RFLP, DNA Sequencing using ABI-3730 automated sequencer, Sequence analysis using Gene tools, Mutant phenotype prediction using Transcription factor binding site search, phylogenetic footprinting analysis, comparative modelling. Statistical analyses include Significance tests, Correlation and regression, power calculations. Protein analytical techniques included Blood cell separation in a dextran gradient, platelet protein preparation, quantitation, Isoelectric Focussing in pre-cast as well as manually developed pH gradient gels, SDS-PAGE, staining to visualize the protein spots, image analysis using PDquest software from BIORAD, preparation of peptides from gel spots for MALDI-TOF MS analysis, analysis of MALDI spectra, database searching to find out the peptide mass fingerprints.
2.M.Tech Dissertation: carried out for a period of six months at Centre for Biotechnology, Anna University, ChennaiTitle:Multivariable optimization of poly--hydroxy butyrate production by Azotobacter beizerinckii by the use of response surface methods.Supervisor: Prof. Kuntala jayaraman and Dr. R.Sachidanandham
Description: Azotobacter beijerinckii is known to grow and produce the biodegradable plastic, poly--hydroxy butyrate (PHB) using defined medium. In the present study, we developed and optimised a process for the production of PHB from molasses and Corn steep liquor (CSL) as the carbon and nitrogen sources. The growth profile of A.beijerinckii was monitored and the product harvesting time was optimised. Experiments were designed using the full factorial design and the results were analysed by the 2D and 3D response surfaces. A mathematical model was developed in terms of total Carbohydrate and Nitrogen content to predict the yield of PHB. This was used to calculate the optimum molasses and CSL concentrations that maximize the yield.
Techniques Used: Basic microbiological methods such as culture maintenance, revival, media preparation, sterilisation, staining, microscopic examination; Bioreactor operation (1lit, 2lit), shake flask batch experiments, PHB extraction using a solvent extraction method, using STATGRAPHICS plus for WINDOWS for experimental design, regression analysis, Design Expert software for studying the response surfaces.
WORK EXPERIENCE
As a Scientist at Dr.Surapanenis Genomic Solutions Pvt Ltd since September 2007 till date. Job responsibilities include gene isolation, cloning, and expression of protein in prokaryotic system, protein purification and modification (fluorescent tagging, enzyme coupling).
TEACHING EXPERIENCE
An experience of two years in Biotechnology and Biochemistry for postgraduate and under graduate students at V.S.Lakshmi Womens College, Kakinada, an affiliated centre to Andhra University. Subjects taught were Intermediary Metabolism, Enzymology, Clinical Biochemistry, Molecular Biology, Industrial biotechnology, Immunology.
RESEARCH EXPERTISE
Biochemistry: Protein purification utilising the affinity chromatography technique, protein estimations, Western Blotting, ELISA, Polyacrylamide Gel Electrophoresis, 2-D gel electrophoresis, image analysis using soft wares like PDQUEST, Proteomic analysis using techniques like MALDI-TOF. Qualitative and quantitative analysis of various classes of bio molecules, enzyme assays and kinetic studies. Evaluation of clinical parameters with special emphasis on biological fluids.
Molecular biology: Isolation of DNA and RNA from cells and tissues, PCR, RT-PCR, agarose and polyacrylamide gel electrophoresis, DNA sequencing and Gene scan.
Microbiology: Culturing of bacterial cells, Staining, basic Bioreactor operations (up to 20L level). Downstream operations involved in product purification.
Computational Skills: Working knowledge of computational tools such as SPSS, Epistat, Statgraphics, MATLAB, Arlequin, GOLD, Swiss PDB modeller, Clustal, Sequence assembling using Auto assembler, Gene tool. Programming knowledge in computer languages such as C and PERL with orientation towards biological applications.
PUBLICATIONS
1. Venkata V Suryanaryana , Lakshmi Rao , Murthy K Kanakavalli , Venkata V Padmalatha , Mamata Deenadayal , Lalji Singh. Role of CYP17 and CYP19 polymorphisms in idiopathic recurrent miscarriages among South Indian women. Reprod Biomed Online. 2007 Mar ;14 (3):341-7.
2. Venkata Suryanarayana, Lakshmi Rao, Murthy Kanakavalli, Venkata Padmalatha, Mamata Deenadayal, Lalji Singh . Recurrent early pregnancy loss and endothelial nitric oxide synthase gene polymorphisms. Arch Gynecol Obstet. 2005 Dec 16: 1-6.
3. K. Anil kumar, K. Lakshmi Rao, Suryanarayana V Vedula, M.K. Kanakavalli, Padmalatha V Vaddamani, Mamata Deenadayal, Nalini Gupta, B.N.Chakravarthy, Lalji Singh (2005). Screening of the galactose-1-phosphate uridyltransferase gene in Indian women with ovarian failure. Reproductive BioMedicine Online, Vol.11 (4), 444448.
4. Venkata Suryanarayana, Mamata Deenadayal, Lalji Singh (2004). Association of CYP1A1 gene polymorphism with recurrent pregnancy loss in the South Indian population, Human Reproduction, vol. 19 (11), 2648-2652.
5. K Arvind Babu, K Lakshmi Rao, M K Kanakavalli, V V Suryanarayana, A P Singh, M Deenadayal and Lalji Singh (2004). CYP1A1, GSTM1 and GSTT1 genetic polymorphism is associated with susceptibility to polycystic ovaries in South Indian women. Reproductive BioMedicine Online, Vol.9 (2), 194200 6. M.Purushothaman, R.K.I.Anderson, S.Narayana, V.K.Jayaraman. (2001) Industrial byproducts as cheaper medium components influencing the production of polyhydroxyalkanoates (PHA)- biodegradable plastics. Bioprocess and Biosystems Engineering. 24 (3), 131-136.
Achievements1.Qualified the National Eligibility Test (NET), held by the Council for Scientific and Industrial Research (CSIR), India in 2000.
2. Awarded Junior Research Fellowships and Senior Research Fellowships by CSIR, India
3.Selected for Jawaharlal Nehru University fellowship to carry out M.Tech at Anna University, Chennai in 1997.
4. Secured 5th rank in the entrance test for admission into M.Sc Biochemistry in the year 1995.
5.Won a best poster award for Detoxification gene polymorphisms- an etiological factor in idiopathic early pregnancy loss (REPL) at 9th ADNAT & 30th ISHG symposium on Molecular Medicine and Health at CCMB, Hyd, INDIA in the year 21-24 Feb 2005.
CONFERENCES AND SYMPOSIA ATTENDED
International Silver Jubilee Symposium (Nov2002) Current Excitement in Biology organized by CCMB, Hyderabad, INDIA
Participated in 7th ADNAT (Association for the Promotion of DNA Fingerprinting and other Technologies), a two day symposium on Proteomics & Micro array at CCMB, Hyderabad, INDIA.
Presented a poster entitled Human Reproductive disorders; Understanding the Genetic basis at C.C.M.B for the opening day in the year Nov 2003.
Participated & presented a poster in CME programme on Current Concepts & Research in Infertility Obstetrics & Gynaecology in Jan 2004 at Owaisi Hospital & Research centre, Hyderabad, INDIA & presented a poster entitled Chromosome defects & Human health.
Participated in 8th ADNAT, a two day symposium on Comparative and Functional Genomics at CCMB, Hyderabad, INDIA in the year 23-24 Feb 2004.
NIRRHISSRF (National Institute for Research in Reproductive Health Indian Society for the Study of Reproduction and Fertility) Symposium on Advances and Challenges in Reproductive Health Research in the Post Genomic Era, January 912, 2005, NIRRH, Mumbai, India
Participated in 9th ADNAT & 30th ISHG (Indian Society of Human Genetics), a four days symposium on Molecular Medicine and Health at CCMB, Hyderabad, INDIA in the year 21-24 Feb 2005.
Participated in 31st annual ISHG conference, a three day symposium held at Jawaharlal Nehru University, New Delhi, INDIA during 27Feb-1Mar 2006.
REFERENCES
Dr. Lalji Singh
Director,
Centre for Cellular and Molecular Biology,
Tarnaka, Habsiguda, Hyderabad, A.P.INDIA
Email: [email protected]: +91-40-27160789
Fax No: +91- 40-27160311
Dr.K.Thangaraj,
Scientist, W 410,
Centre for Cellular and Molecular Biology,
Tarnaka, Habsiguda, Hyderabad, A.P.INDIA
Email: [email protected]
Ph.No: +91-040-27192636
Fax: +91-40-27160591
Ms.K.Lakshmi Rao,
Scientist, E 409,
Centre for Cellular and Molecular Biology,
Tarnaka, Habsiguda, Hyderabad, A.P.INDIA
Email: [email protected]
Ph.No: +91-040-27192607
Fax : +91-40-27160591
I assure that the information that has been given above is true and correct to my knowledge.
V.V.Suryanarayana
