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CV NAME

Alvaro Fernando Acosta-Serrano

PERSONAL DATA Nationality: Venezuelan (UK permanent resident) Date of birth: 30/10/66 Marital status: Single (living with partner); one daughter

CURRENT POSITION

Wellcome Trust Research Fellow (independent investigator) Wellcome Centre for Molecular Parasitology, University of Glasgow, Scotland, UK

OFFICE ADDRESS

Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, Scotland, UK. Tel: 44-141-3305946; Fax: 44-141-330-5422 Emails:afas1u@udcf.gla.ac.uk / afacosta_serrano@hotmail.com

EDUCATION

2000 Johns Hopkins University School of Medicine, Baltimore, U.S.A. Post-doctoral fellow with Prof. Paul T. Englund.

1996 Federal University of São Paulo, São Paulo, Brazil D.Sc. in Molecular Parasitology with Prof. Sergio Schenkman.

1990 "Andrés Bello" Catholic University, Venezuela BE. Major: Biological Sciences.

AWARDS

2003-2007 Wellcome Trust Career Development Fellowship (GB£ 490,000). 2000-2003 Wellcome Trust International Travelling Research Fellowship (GB£ 120,000). 1996-1997 CONICIT (National Research Council of Venezuela) Postdoctoral fellowship (US$

30,000). 1991-1996 CONICIT Graduate studies fellowship (US$ ~100,000). 1994 WHO/TDR/World Bank Fellowship Training Grant in Tropical Diseases (US$

~10,000). 1991 United Nations University Biotechnology fellowship. (US$ ~5,000). 1989 Venezuelan Institute for Scientific Investigations (IVIC) undergraduate research

fellowship.

RESEARCH INTEREST Biosynthesis, structure and function of parasite surface glycoconjugates. General Glycobiology.

RESEARCH EXPERIENCE 2000-2003 University of Dundee, Scotland, UK.

Wellcome Trust Int'l Travelling Fellow with Prof. Michael A. Ferguson, FRS. 1996-2000 Johns Hopkins University School of Medicine, Baltimore, U.S.A.

Post-doctoral fellow with Prof. Paul T. Englund. 1992-1996 Federal University of São Paulo, Brazil.

Graduate student with Prof. Sergio Schenkman.

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1994 University of Dundee, Scotland, UK. WHO visiting student with Prof. Michael A. Ferguson, FRS.

1989-1991 Central University of Venezuela, Caracas, Venezuela. Research Assistant with Prof. José Luis Avila.

1988-89 IVIC, Caracas, Venezuela. Undergraduate student with Dr. Fabian Michelangeli

REFERENCES

Prof. Paul T. Englund. Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205, USA. Tel: +1-410-9553790. E-mail: penglund@jhmi.edu Prof. Michael A.J. Ferguson, FRS. Division of Biological Chemistry and Molecular Microbiology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5HE, Scotland, UK. Tel: +44-1382-344219. E-mail: m.a.j.ferguson@dundee.ac.uk Prof. James D. Bangs. Department of Medical Microbiology and Immunology, University of Wisconsin, 1300 University Avenue, Madison, WI 53706-1532, U.S.A. E-mail: jdbangs@wisc.edu

CURRENT COLLABORATORS Prof. Taroh Kinoshita, Osaka University, Japan. Subject: investigations on the role of trans-sialidases and sialic acids in the trypanosome-tsetse fly interaction. Prof. Michael AJ Ferguson, FRS, Dundee University, Scotland. Subject: structural characterisation of novel T. brucei surface glycoconjugates; functional characterisation of trypanosome GPI mutants. Prof. Peter Butikofer, Bern University, Switzerland. Subject: structural analysis of major GPI surface glycoconjugates from T. congolense insect forms. Dr. Keith Matthews, Edinburgh University, Scotland. Subject: biochemical and functional characterisation of T. brucei ESAG9 proteins.

PAST COLLABORATORS Prof. George AM Cross, FRS. Rockefeller University, USA. Subject: biochemical characterisation of trypanosome mutants obtained by transposition mutagenesis. Prof. Isabel Roditi, Bern University, Switzerland. Subject: expression, structural characterisation and processing of the major surface proteins expressed by T. brucei tsetse fly forms. Dr. James Morris, Clemson University, USA. Subject: isolation and biochemical characterisation of trypanosome glycosylation mutants obtained by a trypanosome RNAi library. Dr. Mark Field, Cambridge University, UK. Subject: biochemical characterisation of T. brucei mutants defective in the endocytic pathway.

TEACHING EXPERIENCE

2005 Invited Lecturer in the course "Glycobiology of Parasites". Department of Cell Biology, Faculty of Science, Universidad de los Andes, Merida, Venezuela.

2002 Tutor in the Cellular and Molecular Biology course for undergraduate students at University of Dundee, Scotland.

1998 Invited Lecturer in the course "Biology of Parasitism". Johns Hopkins School of Hygiene and Public Health, Baltimore, USA.

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1998 Tutor in the course "Molecules and Cells (Biochemistry of Metabolism section)". Johns Hopkins University School of Medicine, Baltimore, USA..

SUPERVISED STUDENTS AND POSTDOCS

2007- Barbara Marchetti. Post-doctoral Research Assistant. 2007- Igor Cestari. Visiting student from Instituto Osvaldo Cruz, Rio de Janeiro, Brazil. 2004-2007 Sylvia Lee. PhD student, Glasgow University (supervisor). 2006- Yuk-Chien Liu. PhD student, Glasgow University (supervisor).. 2006 Priscila Pena. Visiting student from "Los Andes" University, Venezuela. 2001-2003 Jim McRae. PhD student, Dundee University (co-supervisor) 1999 Alfred Ntambi. Undergraduate student, Johns Hopkins University (co-supervisor).

OTHERS: Ad hoc reviewer for EMBO Journal, Eukaryotic Cell, European Journal of Biochemistry, Experimental Parasitology, Glycobiology, Infection and Immunity, Microbes and Infection, Molecular and Biochemical Parasitology, Molecular and Cellular Biology, Parasitology, and Trends in Parasitology. Ad hoc reviewer for The Wellcome Trust. Co-editor of the book 'Trypanosomes — after the genome.' (Barry JD, Mottram J, McCulloch R and Acosta-Serrano A. eds.). Horizon Press. (2007).

PUBLICATION RECORD (listed as Acosta or Acosta-Serrano) Research papers: 1. Avila, J. L., Rojas, M. and Acosta, A. (1991). ‘Glycoinositol Phospholipids from American Leishmania and Trypanosoma spp.: Partial Characterization of the Glycan Cores and the Humoral Immune Response to Them’. J. Clin. Microbiol. 29: 2305 - 2312.

2. Ruiz, MC., Abad, MJ., Gonzalez, B., Acosta, A. and Michelangeli, F. (1993). ‘Comparison of the Control of Acid and Pepsinogen Secretion by Oxyntopeptic Cell of Amphibian’. Acta Cient. Venezolana 44: 89-94.

3. Ruiz, MC., Acosta, A., Abad, MJ. and Michelangeli, F. (1993). ‘Nonparallel Secretion of Pepsinogen and Acid by Gastric Oxyntopeptic Cell of the Toad (Bufo marinus)’. Am. J. Physiol. 265: G934-G941.

4. Acosta, A., Schenkman, RP. and Schenkman, S. (1994). ‘Sialic Acid acceptors of Different Stages of Trypanosoma cruzi are mucin-like glycoproteins linked to the parasite by GPI anchors’. Brazilian J. Med. Biol. Res. 27: 439-442.

5. Amino, R., Acosta Serrano, A., Morita, OM., P. Chioccola, VL. and Schenkman, S. (1995). ‘A Sialidase Activity in the Midgut of the Insect Triatoma infestans is Responsible for the Low Levels of Sialic Acid Present in Trypanosoma cruzi Growing in the Insect Vector’. Glycobiology 5 (6): 625-631.

6. Acosta Serrano, A., Schenkman, S., Yoshida, N., Mehlert, A., Richardson, MJ. and Ferguson, MAJ. (1995). ‘The Lipid Structure of the Glycosylphosphatidylinositol-Anchored Mucin-like Sialic Acid Acceptors of Trypanosoma cruzi changes during parasite differentiation from Epimastigotes to Infective Metacyclic Trypomastigote Forms’. J. Biol. Chem. 270: 27244-27253.

7. Yoshida, N., Dorta, M., Ferreira, A., Oshiro, ME., Mortara, R., Acosta-Serrano, A. and Favoreto Jr., S. (1997). ‘Removal of Sialic Acid from Mucin-like surface molecules of

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Trypanosoma cruzi Metacyclic Trypomastigotes Enhances Parasite-Host Cell Interaction’. Mol. Biochem. Parasitol. 84: 57-67

8. Hwa, K-Y., Acosta-Serrano, A., Khoo, K-H., Pearson, TW. and Englund. PT. (1999). ‘Protein Glycosylation Mutants of Procyclic Trypanosoma brucei: Defects in the Asparagine-Glycosylation Pathway’. Glycobiology 9: 181-190.

9. Acosta-Serrano, A., Cole, RN., Mehlert, A., Lee, MG-S., Ferguson, MAJ. and Englund, PT. (1999). ‘The Procyclin Repertoire of Trypanosoma brucei: Identification and Structural Characterization of the Glu-Pro-Rich Polypeptides’. J. Biol. Chem. 274: 29763-29771.

10. Pereira-Chioccola, VL., Acosta-Serrano, A., de Almeida I., Souto-Padron, T., Rodrigues, MM., Ferguson, MAJ., Travassos, LR and Schenkman, S. (2000). ‘Mucin-Like Molecules Form a Sialylated Coat that Protects Trypanosoma cruzi Trypomastigotes Against Complement Independent Killing Induced by Human Anti-α-Galactosyl Antibodies’. J. Cell. Sci. 113: 1299-1307.

11. Morita, YS., Acosta-Serrano, A., Buxbaum LA. and Englund PT. (2000). ‘Glycosyl Phosphatidylinositol Myristoylation in African Trypanosomes: New Intermediates in the Pathway for Fatty Acid Remodeling’. J. Biol. Chem. 275: 14147--14154.

12. Acosta-Serrano, A., Cole, RN. and Englund, PT. (2000). ‘Killing of Trypanosoma brucei by Concanavalin A: The Structural Basis of Resistance in Glycosylation Mutants’. J. Mol. Biol. 304: 633-644.

13. Leal, S., Acosta-Serrano, A., Morita, YS., Englund, PT., Böhme, U., and Cross GAM. (2001). ‘Virulence of Trypanosoma brucei 427 is not affected by the absence of glycosylphosphatidylinositol phospholipase C’. Mol. Biochem. Parasitol. 114: 245-247.

14. Acosta-Serrano, A., Vassella, E., Liniger, M., Kunz, RC., Brun, R., Roditi, I., and Englund, PT. (2001). ‘The Surface Coat of Procyclic Trypanosoma brucei: Programmed Expression and Proteolytic Cleavage in the Tsetse Fly’. Proc. Natl. Acad. Sci. U.S.A. 98: 1513-1518.

15. Vassella, E., Acosta-Serrano, A., Studer E., Lee S.-H., Englund, PT., and Roditi, I. (2001). ‘Multiple procyclin isoforms are differentially expressed during the development of insect forms of Trypanosoma brucei’. J. Mol. Biol. 312: 597-607.

16. Bütikofer, P., Jelk, J., Malherbe, T., Vassella, E., Acosta-Serrano, A., Kunz Renggli, C., Brun R., and Roditi. I. (2003) ‘Phosphorylation of GPEET procyclin is not necessary for survival of Trypanosoma brucei procyclic forms in culture and in the tsetse fly’. Mol. Biochem. Parasitol 126: 287-291

17. Liniger, M. Acosta-Serrano, A. Van Den Abbeele, J. Kunz Renggli, C., Brun,R., Englund, P.T., and Roditi, I. (2003). ‘Cleavage of trypanosome surface glycoproteins by alkaline trypsin-like enzyme(s) in the midgut of Glossina morsitans’. Int. J. Parasitol. 33: 1319-1328

18. Acosta-Serrano, A., O’Rear, J., Quellhorst, G., Lee, SH., Hwa, KY, Krag, SS., and Englund, PT. (2004). ‘A Trypanosoma brucei Glycosylation Mutant: Defects in the N-linked Oligosaccharide Biosynthetic Pathway’. Eukaryotic Cell 3: 255-263.

19. Nagamune, K., Acosta-Serrano, A., Uemura, H., Brun, R., Maeda, Y., Ferguson, MAJ., and Kinoshita, T. (2004). 'Surface sialic acids taken from the host allow trypanosome to survive in tsetse fly vectors'. J. Exp. Med 199: 1445-1450.

20. Leal, S., Acosta-Serrano, A., Morris, J, and Cross, GAM. (2004) 'Insertional mutagenesis of Trypanosoma. brucei identifies glycosylation mutants resistant to concanavalin A killing'. J. Biol. Chem. 279: 28979-28988.

21. MacRae, JI., Acosta-Serrano, A., Morrice, N., and Ferguson, MAJ. (2005). 'Structural Characterization of NETNES, a higly glycosylated glycoconjugate in Trypanosoma cruzi epimastigotes' J. Biol. Chem. 280: 12201-12211.

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22. Roper, RJ., MacRae, JI., Guther, L., Prescott, AR., Hallyburton, I., Acosta-Serrano, A. and Ferguson, MAJ. (2005). ‘The suppression of galactose metabolism in procyclic form Trypanosoma brucei causes cessation of cell growth and alters procyclin glycoprotein structure and copy numbers’ J. Biol. Chem. 280: 19728-19736.

23. Hall, BS., Arun P., Goulding D., Acosta-Serrano, A., and Field, MC. (2005). 'Trypanosoma brucei: TbRAB4 regulates membrane recycling and expression of surface proteins in procyclic forms'. Exp. Parasitol. 111: 160-171.

24. Utz S., Roditi I., Kunz Renggli, C., Almeida, IC., Acosta-Serrano, A.,and Butikofer P. (2006). 'Trypanosoma congolense procyclin: a novel heavily glycosylated, repetitive surface protein expressed by procyclic forms'. Eukaryotic Cell 5:1430-1440.

25. Guther, MLS., Lee, SY., Tetley, L., Acosta-Serrano, A., and Ferguson, MAJ. (2006). 'GPI-anchored proteins and free GPI glycolipids of procyclic form Trypanosoma brucei are non-essential for growth, are required for colonisation of the tsetse fly and are not the only components of the surface coat'. Mol. Biol. Cell 17:5265-5274.

Review articles: 1. Briones, MRS., Egima, MC., Acosta, A. and Schenkman, S. (1994). ‘Trans-sialidase and Sialic Acid Acceptors from Insect to Mammalian Stages of Trypanosoma cruzi’. Exp. Parasitol. 79: 211-214. 2. Gazzinelli, RT., Camargo, MM., Almeida, IC., Morita, YS., Giraldo, M., Acosta-Serrano, A., Hieny, S., Englund, PT., Ferguson, MAJ., Travassos, LR and Sher, A. (1997). ‘Identification and Characterization of Protozoan Products That Trigger the Synthesis of IL-12 by Inflammatory Macrophages’. Chem. Immunol. 68: 136-152.

3. Acosta-Serrano A., de Almeida I., Freitas Jr., LH., Yoshida N. and Schenkman, S. (2001). ‘The Mucin-like Glycoprotein Super-Family of Trypanosoma cruzi: Structure and Biological Roles’. Mol. Biochem. Parasitol. 114: 143-150.

Book chapters: 1. Morita, YS., Acosta-Serrano, A., and Englund PT. (2000). ‘The Biosynthesis of GPI Anchors’. In: Oligosaccharides in Chemistry and Biology – A Comprehensive Handbook (eds: Ernst, P. Sinaÿ, and G. Hart). Wiley-VCH, Weinheim, Germany, pg. 417-433.

2. Morris, JC., Morris MT., Lee SY., Toole, WP., Seifert, CM and Acosta-Serrano, A.. (2007). 'Reverse and forward genetics as practical approaches for post-genome studies'. in 'Trypanosomes — after the genome.' (Barry, JD., Mottram, J., McCulloch R., and Acosta-Serrano, A., eds.). Horizon Press, UK, pg. 49-70.

3. Acosta-Serrano, A., Hutchinson, C., Nakayasu ES., Almeida, IC. and Carrington, M. (2007). 'Comparison and evolution of the surface architecture of trypanosomatid parasites'. in 'Trypanosomes — after the genome.' (Barry, JD., Mottram, J., McCulloch R., and Acosta-Serrano, A., eds.). Horizon Press, UK, pg. 319-337.

In preparation: - Acosta-Serrano, A., Nagamune, K., MacRae, J., Richardson, J., Kinoshita, T. and Ferguson, MAJ. ‘Structural characterization of a new family of N-Acetyl-lactosamine-rich glycoinositolphospholipids of procyclic form Trypanosoma brucei'.

- Acosta-Serrano, A., Nagamune, Kinoshita, T. and Ferguson, MAJ. ‘Structural characterization of sialylated glycoinositolphospholipids from Trypanosoma brucei GPI10 null cells'.

- Liu, Y.-C. and Acosta-Serrano, A. 'Evidence for the presence of two parallel pathways of GPI synthesis in Trypanosoma brucei insect forms.'

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- Lee SY., Guther, MLS., Barry, JD., Ferguson, MAJ, and Acosta-Serrano, A. 'Mariner transposition generates procyclic form Trypanosoma brucei mutants defective in the first step of glycosylphosphatidylinositol synthesis and unable to survive in tsetse fly vectors'.

- Liu, Y.-C., Hermoso, T., Mottram, J, and Acosta-Serrano, A. 'Complete absence of the glycosylphosphatidylinositol biosynthetic pathway in the human parasite, Trichomonas vaginalis.'

- Seifert, CM., Grubbs A., Morris MT., Acosta-Serrano, A., and Morris, JC. 'A Trypanosoma brucei AMP-dependent kinase links nutrient sensing and surface molecule expression'.

CONGRESS COMMUNICATIONS

Over forty published communications in both Molecular Parasitology and Glycobiology international meetings.

SELECTED ORAL PRESENTATIONS AND INVITED SEMINARS (since 2001)

2007 Department of Biological Sciences, University of Texas at El Paso 2007 24th Swiss Trypanosomatid Meeting, Leysin, Switzerland (opening conference). 2006 Instituto de Biologia Molecular do Parana, Curitiba, Brazil. 2006 School of Biological Sciences, University of Edinburgh, UK. 2006 XXXIII International Congress on Chagas' Disease. Caxambú, Brazil. 2006 School of Pharmacy, Univ. of São Paulo at Riberão Preto, Brazil. 2006 Department of Biochemistry and Molecular Biology, IBLS, Glasgow University. 2004 Fundação Osvaldo Cruz, Rio de Janeiro, Brazil. 2004 Institute of Biomedical Sciences, University of São Paulo, Brazil. 2004 Sialobiology 2004, Saint Andrews, Scotland. 2003 Molecular Parasitology Meeting XIV, Woods Hole, MA. U.S.A.. 2002 The Wellcome Centre for Molecular Parasitology, Glasgow, Scotland. 2001 Department of Biophysics, Max Planck Institute, Gottingen, Germany. 2001 Department of Microbiology, Marburg-Philips University, Marburg, Germany. 2001 XXVIII International Congress on Chagas' Disease. Caxambú, Brazil. 2001 Wellcome Trust, 2001 International Fellow’s Meeting, London, UK. 2001 Molecular Parasitology Meeting XI, Woods Hole, MA. U.S.A.

February 28, 20061. Briones, MRS., Egima, MC., Acosta, A. and Schenkman, S. (