Protein Ark Ltd, The Innovation Centre, 217 Portobello, Sheffield S1 4DP, UK.

For a quotation or further details contact us on:

Telephone:+44 (0) 33 33 44 20 25

Email: info@proteinark.com

or visit our website: www.proteinark.com

About Us

Protein Ark was founded in 2014 and brings together 15 years experience in contract manufacturers and custom services in the UK. Our PhD qualified scientists will provide our customers with the highest quality customised products, services and support.

Facilities

Sheffield: Head office manufacturing, production, testing and technical support

Expertise

Peptide Synthesis Antibody development Molecular biology Protein expression Protein purification Large scale production Analytical technologies

Our guarantee

High quality service Regular updates Competitively priced Scheduled delivery times Troubleshooting and

problem solving Dedicated management

team

Custom Services

Peptide Synthesis

Polyclonal Antibodies

Monoclonal Antibodies

Cloning

Recombinant Protein Services

Bulk Production Services

Antibody production

Protein Ark provides a high quality custom peptide synthesis service with expertise in modified and difficult peptides. Peptide Service: Sequence: 2 to 100 amino acids Purity: Crude to >98% Quantity: Milligrams to 100 grammes Quality: Full mass spec and HPLC analysis Time scale: 2-3 weeks Guarantee: No peptide no fee

Modified and difficult peptides Hydrophobic peptides Non-standard amino acids:

Phosphorylated, D-isomers, methylated, non-natural

Spacers and linkers: PEG, AVA, GABA, AEA, Gly, ANP

Labels: Biotin, FITC, 6-FAM ...and more Cyclic peptides: Disulphide or amide

terminal/side chains N & C terminal Peptide antigens for antibody production Conjugations: KLH, BSA and OVA All our peptides are competitively priced with fast turnaround times and excellent customer support. See our ONLINE ENQUIRY FORM for further details.

Peptide synthesis

Protein Ark provides a fully customised immunisation and antibody production service to meet your needs. Monoclonal Antibody Antigen: Peptide or protein (from

sequence) Peptide: Design, synthesis, conjugation Species: Balb/c Mice Immunisation schedule: 45 day Hybridoma fusion, cloning and cell

banking Screening: ELISA, end-point assay Production: Cell culture Purification: Protein G, affinity

chromatography Deliverable: 2-5 mg purified antibody

and Hybridoma cell line Time scale: 5-6 months Scalability: Gramme quantities

Polyclonal Antibody Antigen: Peptide or protein Peptide: Design, synthesis, conjugation Species: Rabbit, Rat, Guinea Pig Immunisation schedule: 69 day Purification: Protein G, affinity

chromatography Deliverable: 2-10 mg purified antibody Time scale: 3-4 months Guarantee: No antibody no fee

Additional antibody services Phospho-antibody production Testing: WB, IHC, IP Protein free cell line adaptation Fragmentation: Fab and Fab’ generation Produced by experts to ensure the final product is of the highest quality. See our ONLINE ENQUIRY FORM for further details.

Cloning

Protein production

With dedicated teams and extensive experience our UK based facility provides a wide range of high quality services. Expression Service Platform: Bacterial, insect (baculovirus

and stable), mammalian (transient and stable), hybridoma

Screening: strains, clones, media Trial expression: < 0.5 litres Small scale expression: < 10 litres Large scale expression: 50-250 litres Analysis: SDS-PAGE, Western blot

Purification service Un-tagged Tag removal: TEV, enterokinase, thrombin Affinity: polyHis, GST, Biotin, Protein A/G Ion-exchange and size-exclusion

chromatography

Additional services Method development Labelling: Selenomethionine,

selenocysteine, N15

Validated ELISA assay development Endotoxin removal from protein sample Fragmentation: Fab and Fab’ generation Cell line banking and storage

Time scale: 1 litre expression and purification Bacteria: 2-3 weeks Insect cells (baculovirus): 4-5 weeks Insect cells (stable): 6-7 weeks Mammalian: 6-7 weeks

All our production services are performed to ISO 9001 standards to ensure the final product is of the highest quality. See our ONLINE ENQUIRY FORM for further details.

Protein Ark’s dedicated molecular biology team provides a broad range of cloning services and proprietary vectors for successful recombinant protein expression. Cloning Service: Codon optimization and design service Gene synthesis (using GeneWiz) Standard and non-standard restriction

enzyme cleavage Clone into any vector of your choice:

including all standard or licence free vectors

Tags: polyHis, GST, FLAG, V5, Myc, FMDV Time scale: 2-3 weeks Quality assured and sequence verified

Licence free proprietary vectors Based upon same core plasmid

backbone Available for bacterial, insect, and

mammalian expression Standard or custom designed Dual expression versions Modular design Choice of 156 individual components

including: promoters, reporters, antibiotic resistance, origins, tags, signal peptides and more

Save time and money with our fast turnaround sequence verified cloning service. See our ONLINE ENQUIRY FORM for further details.