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♣ 1. Introductory Data:

Position: Prof. of Biochemistry/Molecular Biology, Faculty of Science,

Cairo University.

Date of Birth: 25/3/1965

Gender: Female

Nationality: Egyptian

Marital Status: Married.

Home Address: El Feda Compound - villa 36, 6th of October City, Egypt.

Cell phone:

E-mail: mervat_sayed@yahoo.com

mervat@sci.cu.edu.eg

♣ 2. Employment History:

☻ Employer: Chemistry Department, Biochemistry Unit, Faculty of Science,

Cairo University and Al-Ain University – UAE.

Position

From : To

1- Prof. of Biochemistry / Molecular biology June 2013- Present

2- Associate Prof. of Biochemistry/ Molecular

biology

(2007 – 2013)

Curriculum Vitae

Mervat Sayed Mohamed

2

3- Lecturer of Biochemistry/ Molecular

biology

(2001 - 2007)

4- Assistant Lecturer of Biochemistry- at Al-Ain

University- UAE.

( 1996-2001 )

4- Assistant Lecturer of Biochemistry/

Molecular biology

(1991 – 1996)

5- Instructor of Biochemistry (1986 – 1991)

♣ 3. Educational Qualification:

College/ University Degree Grade

Date

Faculty of Science /

Cairo University.

Ph.D in Biochemistry without 1995

Ph.D Thesis Title:

“Lactoferrin in neutrophil polymorphonuclear leucocytes in

leukaemias ; Biochemical & cytochemical study’’

Faculty of Science /

Cairo University.

M.Sc in biochemistry without 1991

M.Sc Thesis Title: “ Biochemical studies in bilharzial infected mice”

♣ 4. Research Experience:

☻ I have been supervising on many M.Sc and Ph.D theses (nearly 20) in the

fields of Biochemistry , Molecular biology and Biotechnology.

♣ 5. Teaching Experience:

Course No. of Times Level

Proteomic & functional

genomics . 4 Ph.D Biochemistry Students

Cancer Biology 4 M.Sc Students

3

Recent advances in

biotechnology ( industrial

biotechnology/enzyme

engineering )

4 Ph.D Biochemistry Students

DNA technology 10 M.Sc Biochemistry Students

Molecular biology

( gene cloning and

molecular tools)

20

Undergraduate For

1- Biotechnology students

2- Biochemistry students

Tissue Culture 6 M.Sc Biochemistry students

Carbohydrate Metabolism 10

Undergraduate For

1-Biology students

2-Biochemistry students

3- Biotechnology students

Lipid and protein

metabolism 10

Undergraduate For

1- Biology students

2- Biochemistry students

Body fluids 10 Undergraduate For

Biochemistry students

Structure of Biomolecules 8 Undergraduate For

Biochemistry students

Clinical Biochemistry 8

Undergraduate For

1- Biology students

2- Biochemistry students

Vitamins 2 Undergraduate For

Biochemistry students

Practical courses in

molecular biology and

biochemistry

27 years Undergraduates

♣ 6. Laboratory Skills and Techniques:

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siRNA synthesis & Maldi -tof mass spectral.

Molecular Biology: Gene Cloning expression and purification which

includes:

Isolation of Total RNA.

RT-PCR by using gene specific Primer.

Purification of PCR Product.

Gene cloning.

Vector transformation into Bacterial cells.

Induction by IPTG, followed by SDS-PAGE to confirm the

expression.

Detection of expressed protein by Western blot technique.

PCR: Primer Designing for PCR.

Protein purification: Purification of recombinant protein

Electrophoresis Techniques: Electrophoresis of DNA/RNA on Agarose

gel and electrophoresis of protein on SDS-PAGE.

Microbiological Techniques:

Competent cell preparation.

Transformation and preparation of culture media.

Biochemical Techniques:

Estimation of concentration of DNA, RNA, Protein and

enzymes.

Chromatography techniques, Paper, Column and HPLC.

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Immunological techniques: Immunocytochemistry and ELISA.

Microscopic technique: Confocal Microscopy.

Computer Skills:

Microsoft Office.

Primer designing.

Gene sequence analysis.

Retrieval of data from various databases.

Gene Scan and protein databases.

Phylogenetic tree.

Gel Documentation Program.

♣ 7. Management Experience:

Member of the editorial/advisory board of the Current World

Environment (CWE) journal published by Oriental Scientific Publishing

Company, Asia ISSN, 0973-4929.

Coordinator of biotechnology program (special program at Faculty of

Science, Cairo University, 2008 -2009). My main role was developing the

program protocol and following up its progress. The program ranked first

in the Quality Assurance and Accreditation Center in Egypt.

Coordinator of Ph.D program of Biochemistry, Cairo University, Faculty of

Science (2010 - present).

♣ 8. Scientific Consultation:

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Organization Name Type of Consultation Period of

Consultation

Cairo University Supervisor of Central Lab 2006-2009

Cairo University Development of biotechnology

curriculum

2008-2009

♣ 9. Training Experience:

Training attended Place Date

Oligonucleotide synthesis and its

usage as RNA antisense

Ulm University, Ulm, Germany 2004

A contributor in the workshop of

DNA technology

Cairo University 2005

Two months workshop in Protein

Crystallization and Drug Design

Cairo University 2006

Two months training in

bioinformatics

Sira, Egypt 2007

♣ 10. Conferences:

A- International:

Conference Name Topic Date and Place Type of participation

3rd

conference of

Synchrotron

Azurin as

Antitumor Protein

and its Effect on the

Cancer Cell Lines

2007, Jordan Project Poster

B- Local:

Conference Name Topic Date and Place Type of participation

5th

International

Conference of

Chemistry

Degradation of

methomyl by the

novel bacterial strain

Stenotrophomonas

maltophilia M1

2010, Egypt Speaker

4th

conference of 4th

conference of 2008, Egypt Poster

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Synchrotron Synchrotron

4th

international

conference of

Chemistry

Isolation and

characterization of

novel bacterial

strain from

Egyptian lakes

capable of

degrading the

widely used

pesticide, methomyl

2006, Egypt Speaker

53rd

conference of

Laboratory

Medicine

Prophylactic and

curative effect of

zingiber officinale

against

hyperlipaemia in

albino rats.

2005, Egypt

Poster

♣ 11. Projects:

Title of research Year Fund and Participation

“Screening for Novel Pesticides

Degrading Bacteria, Cloning, and

Molecular Characterisation of

Gene(s) Involved in Pesticides

degradation”

2007 Funded by Cairo University

♣ 12. International Journals (with high impact factors):

Author at:

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Clinica Chimica Acta.

Clinical Biochemistry.

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.

Australasian Plant Pathology.

Electronic Journal of Biotechnology.

African Journal of Biotechnology.

Indian Journal of Clinical Biochemistry.

• Applied Biochemistry Biotechnology.

Journal of Genetic Engineering and Biotechnology

Mermber at :

Middle East Molecular Biology Society

♣ 13. List of Publications:

1. Mohamed A. Ragheb, Mohamed A. Eldesouki, Mervat S. Mohamed : DNA

binding, photo-induced DNA cleavage and cytotoxicity studies of lomefloxacin

and its transition metal complexes (2015). Spectrochimica Acta Part A:

Molecular and Biomolecular Spectroscopy . 138 ; 585–595.

2. Magda F. Mohamed, Mervat S. Mohamed, Mohamed M. Fathib, Samia A.

Shoumanc and Ismail Abdelshafy Abdelhamid (2014). Chalcones Incorporated

Pyrazole Ring Inhibit Proliferation, Cell Cycle Progression,Angiogenesis and

Induce Apoptosis of MCF7 Cell Line. Anti-Cancer Agents in Medicinal

Chemistry. 14; 1282-1292.

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3- T. A. A. Moussa1, M. S. Mohamed and N. Samak . Production and

characterization of Di-Rhamnolipid produced by Pseudomonas aeruginosa

TMN ( 2014 ) . Brazilian Journal of Chemical Engineering. 31(4) ; 867 – 880.

4. Emad F Eskander, Ahmed A Abd-Rabou, Mervat S. Mohamed, Shaymaa

MM Yahya, Ashraf El Sherbini, Olfat G Shaker ( 2014 ) : The Potential Impact

of P53 and APO-1 Genetic Polymorphisms on Hepatitis C Genotype 4a

Susceptibility. Gene. 15; 550(1):40-5.

5. Emad F. Eskander, Ahmed A. Abd-Rabou, Shaymaa M. M. Yahya, Ashraf El

Sherbini, Mervat S. Mohamed , Olfat G. Shaker ( 2014 ) : The Impact of

Pegylated-Interferon-α plus Ribavirin on Prolactinemia and Testosteronemia

among Hepatitis C-Genotype-4a Patients , Open Journal of Biochemistry: 2 (1)

: 1-8.

6. Emad F. Eskander , Ahmed A. Abd-Rabou , , Shaymaa M. M. Yahya ,

Mervat S. Mohamed ,Olfat G. Shaker ( 2014 ): P53 Codon 72 Single Base

Substitution in Viral Hepatitis C and Hepatocarcinoma Incidences . Indian

Journal of Clinical Biochemistry: 29 (1): 3-7

7- Mohamed A. 1 Eldesouki, Nevin E. Sharaf, Alia A. Abdel Shakour,

Mervat S. Mohamed, Amal S. Hussein and Ibrahim W. Hasani ( 2013): Study

of the Effect of Occupational Exposure to Volatile Organic Compounds

(VOC’s) on Male Reproductive Hormones. World Journal of Medical Sciences

8 (1): 06-12.

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8- Emad F. Eskander, Ahmed A. Abd-Rabou, Shaymaa M. M. Yahya, Ashraf

El Sherbini, Mervat S. Mohamed, Olfat G. Shaker ( 2013 ): Correlation and

Multiple Regression Analyses of Pituitary Growth Hormone and Hepatic

Activities in Hepatitis C Infection and Interferon Response. Indian Journal of

Clinical Biochemistry . 28(4): 348-357.

9. Magda F. Mohamed , Mervat S. Mohamed , Samia A. Shouman , Mohamed

M. Fathi &Ismail Abdelshafy Abdelhamid ( 2012 ): Synthesis and Biological

Evaluation of a Novel Series of Chalcones Incorporated Pyrazole Moiety as

Anticancer and Antimicrobial Agents. Appl Biochem Biotechnol , 168(5 )

: 1153-1162 .

10. Emad F. Eskander a, Ahmed A. Abd-Rabou , Shaymaa M.M. Yahya , Olfat

G. Shaker , Mervat S. Mohamed ( 2012 ): Does interferon and ribavirin

combination therapy ameliorate growth hormone deficiency in HCV genotype-

4 infected patients? Clinical Biochemistry ,45: 3–6

11. Nadia I. Zakhary, Mervat S. Mohamed, Ola Khorshid ,Remon S. Azer ,

Naglaa Zayed: (2012) . Role of glypican-3 in the early diagnosis of

hepatocellular carcinoma among Egyptian patients , Journal of Genetic

Engineering and Biotechnology ,10: 73–79.

12. Soliman A. M. , Amal A. Ahmed1 , Mervat S. Mohamed , Demiana H.

Hanna ; B. N. Barsoum (2012): Gazar virus Y, a new member of the celery

mosaic virus group of potyviruses, isolated from carrots in EGYPT:

Australasian Plant Pathology, 41 (5) : 529-534.

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13. Emad F. Eskander, Mervat S. Mohamed, Ahmed A. Abd-Rabou, Shaymaa

M. M. Yahyaa, Olfat G. Shaker (2012): Does HCV Patients Who Have BCL2

43Ala Genotype and Normal GH1 Levels Can Achieve Response to IFN Based

Therapy? Indian Journal of Clinical Biochemistry, 27(4) : 344-350.

14. Mervat S. Mohamed, Azza A. Shoukry, and Ayat G. Ali (2012) : Synthesis and

structural characterization of ternary Cu (II) complexes of glycine with 2,2′-

bipyridine and 2,2′-dipyridylamine. The DNA-binding studies and biological

activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 86:

562- 570 .

15.Azza A. Shoukry & Mervat S. Mohamed ( 2012 ) : DNA-binding,

Spectroscopic and antimicrobial studies of Palladium (II) complexes containing

2, 2΄-bipyridine and 1-phenylpiperazine : Spectrochimica Acta Part A: Molecular

and Biomolecular Spectroscopy , 96 : 586–593

16. Olfat G. Shaker , Emad F. Eskander , Shaymaa M.M. Yahya, Mervat S.

Mohamed and Ahmed A. Abd-Rabou (2011): Genetic variation in BCL-2 and

response to interferon in hepatitis C virus type 4 patients : Clinica Chimica Acta

, 412 : 593–598

17. Mervat S. Mohamed , Walid Abu El-Soud and Magda F. Mohamed

(2011): Cloning and expression of the recombinant NP24I protein from tomato

fruit and study of its antimicrobial activity : African Journal of Biotechnology,

10(65), 14276-14285

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18. Mervat S. Mohamed , Salwa F. Sabet , Mohamed M. Moustafa and

Mohamed S. Salama (2011) : Correlation between a single nucleotide

polymorphism (G/T at nt –88) in the Mx1 gene promoter and the response to

interferon therapy for hepatitis C virus in Egyptian patients : African Journal of

Biotechnology, 10(62): 13376-13382

19. Mervat S. Mohamed , Said A. Fattah and Howayada M. Mostafa (2010 ):

Azurin as Antitumor Protein and its Effect on the Cancer Cell Lines : Current

Research Journal of Biological Sciences.

20. Mervat S. Mohamed ( 2009) : Degradation of Methomyl by the Novel

Bacterial Strain Stenotrophomonas maltophilia M1 . Electronic Journal of

Biotechnology, 12 (4) .

21. Goda S. K., Mohamed M.S. and Rashedi F.B. (2007): Isolation and

structure determination of two naturally inhibitors for salivary alpha-amylase, a

possible role as hypoglycemic drugs. Arab J. Lab. Med., 33 (1): 83-92.

22. Mohammad A.M., Mohamed M.S., Attaby F.A. and Abo-Taleb H. (2007):

N-acetyl transferase 2 gene polymorphism in bladder cancer. Arab J. Lab.

Med., 33 (1) : 93-101.

23. Mervat S. Mohamed ; Ahmed S, Abd-Allah; Nagwa S, Abd-Allah,

Manal El-Deeb; Soad Sultan and Adel S, Mohamed. (2007): Role of Serum

Interleukin -6 and Osteocalcin in Prediction of Bone Loss in Postmenopausal

Women. Med.J.Cairo univ.,75(1) ,Suppl.March:117-121.

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24. Mervat S.Mohamed (2006): Isolation and characterization of novel

bacterial strain from Egyptian lakes capable of degrading the widely used

pesticide, methomyl. Biosciences, Biotechnology Research Asia , 3(1a): 17-22.

25. Said,M.M.; El Sayed, A.S. Mervat S. Mohamed and Maria , F.Elmhdwi.

(2006): Biochemical evaluation of Ginkgo biloba extract (EGB) in Albino

Rats. 53 rd

Sceintific Congress, International Meeting, Cairo, Egypt (accepted

for publication)

26. El-Kannishy S.M. , Mohamed M.E. , Abo Hashem E.M and Riad B.Y.

(2005): The state of plasma nitric oxide metabolites (N02- and N0 3

- ) and

endothelin-1 in patients with chronic renal failure and/or ischemic heart

disease. Arab J. Lab. Med. , 31 (1): 49-62.

27. Said, M. M.; El Sayed, A. S. ; Mohamed, M.E. and Maria, F.Elmhdwi.

(2005): Prophylactic and curative effect of zingiber officinale against

hyperlipaemia in albino rats. J.Drug Res. Egypt, 26 (1):28-37.

28. El-Kannishy S.M. , Mohamed M.E. , Abo Hashem E.M and Riad B.Y.

(2005): The state of the major circulating apolipoproteins in patients with

chronic renal failure and/or ischemic heart disease. Arab J. Lab. Med., 31 (1):

31-47.

29. Mervat S. Mohamed, A.E.Zakaria, B.Y.Riad and N.S.El-Gendy. (2005):

Isolation and characterization of potent biodesulfurizing microorganisms from

the Egyptian environment. Egypt.J.Biomed.Sci., 19 :305-315.

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30- El-Kannishy S.M. , Mohamed M.E. , Abo Hashem E.M and Riad B.Y.

(2005). Hyperhomocysteinemia in chronic renal failure is an evolving risk

factor for ischemic heart disease. Med.J.Cairo Univ. ,73 (1):163-170.

♣ 14. Manuscripts in preparation:

Mervat S. Mohamed: Production and Structural characterization of Di-

rhamnolipid produced by Pseudomonas aeruginosa TMN strain isolated from

Compost pile waste, Egypt

♣ 15. Language skills:

Arabic: Mother Tongue

English: Excellent in reading & writing. Very good in speaking

English: Excellent in speaking scientific language .

French: Fair

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