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Fig.1. Colony Characteristics on Agar Plates

Fig. 2. Surface Growth Patterns In Broth Medium

Fig. . Agar Slant Growth Patterns

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G'()P *#+

Cultural Characteri,ation of Bacteria

Agar Plate - refer to g. 1/1. Amount# = 0, slight = +, moderate = ++, abundant = +++2. Si,e# Pinpoint, small, moderate or large.. Surface#

a. concentric(ring-like pattern)0.rugose(wrinkled)c.contoured(smooth fold-like pattern)d.radiate(spoke-like pattern)e.smooth

+. Pigmentation# Color of colon. Form#

a. Circular ! "nbroken, peripheral edge0. Irregular ! #ndented, peripheral edgec. 'hi,oid $oot-like spreading growthd.Punctiform- %er minute, pin-pointe. Filamentous 3&hreadlike

4. Margin#&he appearance of the outer edge of the colon .a. curled- concentric e%en or irregular rings0. "ntire ! 'mooth 'harpl dened, e%enc. 5o0ate arked indentations, lobes with deeper indentations than

undulate

d. )ndulate ! regular wa%e-like pattern , wa% indentationse. Serrate &ooth-like appearancef. Filamentous &hreadlike, hair, spreading edge

6. "le7ation#&he degree to which the colon growth is raised on the agarsurface

a. Flat *le%ation not discernable0. 'aised 'lightl ele%atedc. Con7e8 ! ome-shaped ele%ationd. )m0onate ! $aised with ele%ated con%e central regione. Pul7inate- higher dome than con%ef. Su0surface(pour plates onl)- these are spindle-shaped colonies

&u0e Culture -Broth/ - refer to g. 2/&hese are e%aluated as to the distribution and appearance of the growth

1. )niform ne tur0idity nel dispersed growth throughout2. Flocculent ! lak aggregates dispersed throughout. Pellicle &hick pad-like growth on the surface+. Sediment Conc. of growth at the bottom of broth (ma be granular,

ak/ occulent. )

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&u0e Culture -Slant/&hese ha%e single straight line of inoculation on the surface.

1. Amount of growth# none= 0, slight = +, moderate = ++, abundant = +++

2. Pigmentation# color

. (9tical characteristics#a. o9a:ue(no light transmission)0. translucent(partial transmission)c. trans9arent(full transmission).

+. Form# (refer to g. )a. Filiform Continuous, threadlike growth with smooth edges.0. "chinulate Continuous, threadlike growth with irregular edges.c. Beaded 1oncouent to semicouent coloniesd. ";use &his, spreading growthe. Ar0orescent &ree-like growthf. 'hi,oid $ootlike growth

&u0e culture -Butt3Slant/1. Amount- none= 0, slight = +, moderate = ++, abundant = +++2. Form- (refer to ig..). Consistency+. Color. Growth along the Sta00ed 5ine- etend outside the line (motile), stas

in the line (unmotile)

&u0e Culture -Butt/&his solid medium ma be li2ueed b the en3matic action of gelatinase. &hisli2uefaction occurs in a %ariet of patterns4

1. Crateriform li2ueed surface is saucer-shaped.2. !a9iform 5ulbous-shaped li2uefaction. Infundi0uliform ! funnel-shaped+. Saccate *longated, tubular. Stratiform Complete li2uefaction of the upper half of the medium4. Motify6. Gas39roduction