Cultivation of viruses
description
Transcript of Cultivation of viruses
![Page 1: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/1.jpg)
![Page 2: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/2.jpg)
![Page 3: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/3.jpg)
CultivationCultivation
To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.
![Page 4: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/4.jpg)
Cultivation
Bacteria
Plant
AnimalInsect
![Page 5: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/5.jpg)
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
![Page 6: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/6.jpg)
Chick embryo technique Chick embryo technique
![Page 7: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/7.jpg)
![Page 8: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/8.jpg)
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
![Page 9: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/9.jpg)
Cell cultureCell culture
1. Removal.2. Homogenization.3. Washing.4. Trypsinization.5. Washing.6. Growing.7. Inoculation.8. Incubation.
![Page 10: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/10.jpg)
![Page 11: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/11.jpg)
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
![Page 12: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/12.jpg)
IntracerebrallyIntracerebrally
IntraperitoneallyIntraperitoneally
IntramuscularlyIntramuscularly
IntravenouslyIntravenously
IntranasallyIntranasally
![Page 13: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/13.jpg)
Types of cell culture
Primary
Diploid
Suspension Permanent
![Page 14: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/14.jpg)
Primary cell culturePrimary cell culture
A culture derived directly from a tissueA culture derived directly from a tissue
![Page 15: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/15.jpg)
Diploid cell cultureDiploid cell culture
Subsequent cultures derived from primary cell culture.Subsequent cultures derived from primary cell culture.
![Page 16: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/16.jpg)
Suspension cell cultureSuspension cell culture
A culture in it the dispersed cells are not allowed to settle down, but A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.they are kept floating by continuous shaking of the flasks.
![Page 17: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/17.jpg)
Permanent cell linePermanent cell line
A culture derived from single separated cell i.e. diploid culture it can be A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely. propagated indefinitely.
![Page 18: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/18.jpg)
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
![Page 19: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/19.jpg)
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
![Page 20: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/20.jpg)
InoculationInoculation
IncubationIncubation
InoculationInoculation
IncubationIncubation
![Page 21: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/21.jpg)
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
![Page 22: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/22.jpg)
![Page 23: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/23.jpg)
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
![Page 24: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/24.jpg)
IncubationIncubation
![Page 25: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/25.jpg)
Cultivation of bacterial Cultivation of bacterial virusesviruses
1.1. On broth mediaOn broth media 2. On solid medium2. On solid medium
![Page 26: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/26.jpg)
Bacteria + solid agarBacteria + solid agar
Virus + semisolid agarVirus + semisolid agar
![Page 27: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/27.jpg)
Cultivation of Insect virusesCultivation of Insect viruses
![Page 28: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/28.jpg)
![Page 29: Cultivation of viruses](https://reader035.fdocuments.in/reader035/viewer/2022081503/56815d21550346895dcb1d79/html5/thumbnails/29.jpg)
Criteria of purityCriteria of purity
Chemical purityChemical purity
Physical purityPhysical purity
Biological purityBiological purity