CULTIVATION OF ULVA IN AQUACULTURE EFFLUENT A preliminary investigation
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Transcript of CULTIVATION OF ULVA IN AQUACULTURE EFFLUENT A preliminary investigation
D. V. Robertson-Andersson; A. Govender; T. Probyn; C. Halling; M. Troell; J. Bolton & R. Anderson
CULTIVATION OF ULVA IN AQUACULTURE EFFLUENT
A preliminary investigation
Recirculation Council Meeting 2004
INTRODUCTION
• Previous studies in Israel here have shown that Ulva lactuca is capable of removing significant amounts of nutrients (60 – 85%) from aquaculture effluents
• Modelling these systems is built on vertical integration (i.e. not closed systems)
• Models of such systems use whole farm approaches
Benefits of a closed system
• Increase in temperature above ambient seawater equates to an increase in growth rates
• Decrease in pumping costs
• Additional safety in times of red tide and oil spills
Why cultivate seaweeds on abalone farms?
• Mixed diet gives better growth rates
• MSY of kelp bed reached in 2002
•Potential over-harvesting
•Decrease in epiphyte densities
• Limited suitable coastal areas
• ‘HAB’s’
• Recirculation
• Integration will improve water quality
AIMS 2000 - 2003
1) Feasibility of growing commercially useful amounts of Gracilaria and Ulva
2) Influence of different growth media (seawater, abalone and turbot effluent water and fertilized seawater) on growth rates and yields.
3) Seasonal changes in tissue N and P content
4) Relationships between stocking density and yield
5) Uptake rates of various nutrients at differing stocking densities
AIMS 2004 - 2006
1) Investigate and model the physico-chemical dynamics of a farm section
2) Influence of different diet on abalone growth health and excretion rates in a closed system compared to a flow through system
3) Seasonal changes in the above
4) System environmental quality
PROJECT SITES
Abalone mariculture farms
• Danger Point (I & J Mariculture Farm) + 140 km east of CT
• Jacobs Baai (Jacobs Baai Sea Products) + 120 km N of CT
0
1
2
3
4
5
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8
sea shaded sea fert
shaded fert abalone shaded abalone
SG
R %
day
-1
J J A S O N D J F M A M J J A S O
2
RESULTS OF 2000 - 2003
SGR of Ulva from June ‘01 to October ‘02 in sea, fertilized & abalone effluent water. (2) where water exchanges increased at I & J
RESULTS
RGR of Ulva from June ‘01 to October ‘02 in sea, Turbot & abalone effluent water at JSP.
0
2
4
6
8
10
12
14
28-Jul 16-Sep 5-Nov 25-Dec 13-Feb 4-Apr 24-May
13-Jul 1-Sep 21-Oct 10-Dec
SG
R (
%.d
)
Small sea Medium sea
RESULTSMyrionema strangulans (MS)
• Brown spots
• Numbering between 5 and 10 on the holdfast section of Ulva thalli
• The spots are regular discs, 1 – 3 mm in diameter
• First record for South Africa
• Identified by Dr. Herre Stegenga
• Negative correlation between infection density and SGR
BAD INFECTION
DEAD
RESULTSMyrionema strangulans (MS)
HEALTHY
INFECTED
RESULTS
MS infestation of Ulva thalli
y = -0.4557x + 3.2565
R2 = 0.2605
0
2
4
6
8
0 1 2 3 4 5 6
B-B scale of infection
SGR
(% D
ay)
RESULTS
Are important because:
• Low water exchange rate leads to:
• Carbon and nutrient limitation
• Poor condition of thalli (bleached & broken)
• Low Nitrogen content
• Low SGR
therefore low yield
Measures
• Carbon limitation in seaweeds can be shown by an increase in pH values
FLOW RATES (4, 12 & 20)
RESULTSSHADING
Important because
• Shading helps to reduce epiphytes
• Helps in decreasing MS infestations
• Helps to increase tissue N and P
How?
• Tanks should be shaded from September to January
• Shade cloth must be 20 % not 50 %
RESULTS
Important because:
• Increases protein from wild harvest (3.7- 24 % wild to 49.8 % cultured)
• Abalone receive more protein
• Faster growth rates
How do you increase tissue nutrients?
• Faster flow rates (12 – 20 volume exchanges)
• Grown in turbot/fish or abalone effluent
• Add Fertilizer and Kelpak®
Tissue Nitrogen
RESULTSTissue Nitrogen vs. Thallus Colour
Tissue nitrogen vs. thallus colour
RESULTSTissue Nitrogen vs. Tissue Phosphorus
Tissue N vs. Tissue P
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10
20
30
40
50
Time
Rat
io o
f N:P
w ild stock turbot abalone
Small sea Medium sea
A S O N D J F M A M J J A
PROJECT DESIGN AT I & J 2004
• 2 Ulva tanks (5 X 1 X 0.63 m) were integrated to one abalone tank (7 X 2 X 1 m)
• Run under normal farm operating conditions
• Control are 3 flow through abalone tanks
• Seaweeds initial stocking density 2.5 kg.m-2
• Harvested every two weeks
• + 13 200 abalone (10 – 15g), total biomass 165 kg
• 20 % fresh seawater input (800 L.h-1)
• 80 % recirculated through seaweeds (3 200 L.h-1)
Longitudinal profile of tanks
Transverse profile of tanks
5 m
1 m
Longitudinal profile of tanks
Transverse profile of tanks
Seaweed tank Seaweed tank
PumpPump
Gravity feed Gravity feed
Abalone tanksAbalone tanks
Seaweed tank Seaweed tank
80 % recirculation 80 % recirculation
20 % Sea water 20 % Sea water
Over flow Over flow
RESULTS
In this systemIn this system:
• Ulva can take up 90 % of ammonium during the day in abalone effluent at 12 volume exchanges per day
• DO values don’t go below 9 mg.l-1 at night
• Using stocking density of 3 kg.wwt.m-2 gives best uptake rates in the existing system
• Toxic ammonia never reaches levels harmful to abalone at above stocking density
Seaweed uptake
RESULTS
• Ammonium levels must remain below 0.15 uM N.L-1
• DO values between 2 and 12 mg.L-1, preferred 4 - 9
• Temperature range 9 – 22 °C, 16 – 18 °C preferred
• pH 6.5 – 8.4, 7.6 - 8.2 preferred
Abalone critical points
Balancing the equations
Using linear mass balanced equations
Multiple regression of factors
Problems putting theory and reality together
RESULTSDissolved oxygen
Dissolved oxygen in a recirculation system
5.0
7.0
9.0
11.0
13.0
incoming sea seaw eed3
15 16 20 00 04 08 12 16 20 00 04 08 12 16
RESULTSDissolved oxygen
Dissolved oxygen in a recirculation system
5.0
7.0
9.0
11.0
13.0
incoming sea Recirc seaw eed3
15 16 20 00 04 08 12 16 20 00 04 08 12 16
RESULTS
pH in a recirculation system
pH
7.0
7.5
8.0
8.5
9.0
incoming sea seaw eed3
15 16 20 00 04 08 12 16 20 00 04 08 12 16
RESULTS
pH in a recirculation system
pH
7.0
7.5
8.0
8.5
9.0
incoming sea Recirc seaw eed3
15 16 20 00 04 08 12 16 20 00 04 08 12 16
RESULTS
Temperature in a recirculation system
Temperature
7.0
12.0
17.0
22.0
27.0
incoming sea seaw eed3
15 16 20 00 04 08 12 16 20 00 04 08 12 16
RESULTS
Temperature in a recirculation system
Temperature
7.0
12.0
17.0
22.0
27.0
incoming sea Recirc seaw eed3
15 16 20 00 04 08 12 16 20 00 04 08 12 16
RESULTS
ammonium levels in recirculated tanks range between
0.003 - 0.005 uM N
ammonium levels in abalone flow through tanks
= 0.01 uM N
Ammonium levels
Major Challenges
For farm management of such a system:
• Diel relationship between oxygen and pH in the seaweed systems.
• As the seaweeds start to respire at night, abalone experience an increase in oxygen demand, due to night time feeding activity and excretion in the abalone systems, which has implications for the management of ammonia.
• Seaweed uptake decreases at night when abalone excretion reaches a maximum
• Where is the missing oxygen?
The future
• Run this study over a 18 months to obtain seasonal
changes
• Effect of diet on growth and health
• Health of abalone in an integrated closed system vs.
normal farm flow through conditions
THANK YOU
ACKNOWLEDGEMENTS
I would like to extend special thanks to the following people and organizations without whose help this
project would be impossible:
I & J Mariculture farm particularly N. Loubser, H. Otto and L. Ansara
JSP Mariculture farm particularly K. Ruck
N R F
Swedish and South African Collaborative Program
Kelp Products
JSP