CTCF P68 DNA Merge - Genes &...
11
CTCF P68 DNA Merge Yao_Supplemental Fig1.
Transcript of CTCF P68 DNA Merge - Genes &...
CTCF P68
DNA Merge
Yao_Supplemental Fig1.
CTCF
0
0.2
0.4
0.6
0.8
1
1.2
CtrlshRNA
CTCFshRNA
p68shRNA
p68
0
0.2
0.4
0.6
0.8
1
1.2
CtrlshRNA
CTCFshRNA
p68shRNA
A
C
Rela
tive
RNA
expr
essi
onR
elat
ive
RN
A ex
pres
sion
0
0.20.4
0.60.8
11.2
shRNACTCF
shRNAp68
shRNA
Rela
tive
RNA
expr
essi
on
Ctrl
0
0.2
0.4
0.6
0.8
1
1.2
CtrlshRNA
CTCFshRNA
p68shRNA
Rel
ativ
e R
NA
exp
ress
ion
Mouse
Mouse
0
0.25
0.5
0.75
1
1.2
CtrlsiRNA
SRAsiRNA
E
Rela
tive
RNA
expr
essi
on
SRA
CTCF
p68
Human
Human
Human
D
B
Yao_Supplemental Fig2.
0
0.2
0.4
0.6
0.8
1
1.2
1.4
CtrlshRNA
CTCFshRNA
p68shRNA
CtrlshRNA
CTCFshRNA
p68shRNA
Rel
ativ
e p6
8 ex
pres
sion
p68
pIHLIE pIHLME
CTCF
0
0.2
0.4
0.6
0.8
1
1.2
1.4
CtrlshRNA
CTCFshRNA
p68shRNA
CtrlshRNA
CTCFshRNA
p68shRNA
Rel
ativ
e C
TCF
expr
essi
on
pIHLIE pIHLME
CtrlsiRNA
SRAsiRNA
CtrlsiRNA
SRAsiRNA
0
0.25
0.5
0.75
1
1.25
1.5
pIHLIE pIHLME
Rel
ativ
e S
RA
expr
essi
on SRA
C
B
A
Yao_Supplemental Fig3.
CTCF
00.2
0.40.60.8
1
1.21.4
CtrlshRNA
CTCFshRNA
CtrlshRNA
CTCFshRNA
Rel
ativ
e C
TCF
expr
essi
on
p68
0
0.20.4
0.60.8
11.2
1.4
CtrlshRNA
p68shRNA
CtrlshRNA
p68shRNA
Rel
ativ
e p6
8 ex
pres
sion
CtrlsiRNA
SRAsiRNA
CtrlsiRNA
SRAsiRNA
pNI pNI-core
0
0.25
0.5
0.75
1
1.25
1.5
Rel
ativ
e S
RA
expr
essi
on
pNI pNI-core
pNI pNI-core
SRA
A
C
B
Yao_Supplemental Fig4.
0
0.25
0.5
0.75
1
1.25
1.5
CtrlsiRNA
p72siRNA
CtrlsiRNA
p72siRNA
Rel
ativ
e N
eo e
xpre
ssio
n
pNI pNI-core
Yao_Supplemental Fig5.
0
0.02
0.04
0.06
0.08
0.1
0.12
chr1:
38,22
8,447
chr2:
89,40
7,391
chr6:
33,74
8,701
chr8:
27,52
8,032
chr9:
135,2
08,84
1
chr10
:113,8
87,22
8
chr11
:6,66
0,882
chr12
:54,79
7,993
chr14
:104,5
13,67
1
Per
cent
age
of in
put
IgG p68
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
2
Per
cent
age
of in
put
Rabbit serum CTCF
A
B
Yao_Supplemental Fig6.
-actina
-p68a
Ctrl
shR
NA
p68
shR
NA
-CTCFa
0
0.02
0.04
0.06
0.08
0.1
0.12
Rabbit serum CTCF
Ctrl shRNAp68 shRNA
0
0.005
0.01
0.015
0.02
0.025
0.03
IgG p68
Ctrl shRNAp68 shRNA
Per
cent
age
of in
put
Per
cent
age
of in
put
A
B
C
Yao_Supplemental Fig7.
0
0.4
0.8
1.2
1.6
2
Ctrl shRNA p68 shRNA CTCF shRNA
Relative mRNA expression
Yao_Supplemental Fig8.
SRA
Rad21
0
0.5
1
1.5
2
Ctrl shRNA p68 shRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n
SA2
0
0.5
1
1.5
2
Ctrl shRNA p68 shRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n
SMC1
0
0.5
1
1.5
2
Ctrl shRNA p68 shRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n SMC3
0
0.5
1
1.5
2
Ctrl shRNA p68 shRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n
Rad21
0
0.5
1
1.5
2
Ctrl siRNA SRA siRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n
SMC1
0
0.5
1
1.5
2
Ctrl siRNA SRA siRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n
SMC3
0
0.5
1
1.5
2
Ctrl siRNA SRA siRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n
SA2
0
0.5
1
1.5
2
Ctrl siRNA SRA siRNA
Rel
ativ
e m
RN
A e
xpre
ssio
n
A
B
(a) (b)
(c) (d)
(a) (b)
(c) (d)
Yao_Supplemental Fig9.
-SA2
-actinCt
rl sh
RNA
SA2
shRN
A-1
SA2
shRN
A-4
SA2
shRN
A-3
SA2
shRN
A-2
SA2
shRN
A-5
1 2 3 4 5 6
α
α
A
B
p68
0
0.2
0.4
0.6
0.8
1
1.2
1.4
Ctrl shRNA SA2shRNA-3
SA2shRNA-5
Rel
ativ
e m
RN
A e
xpre
ssio
n
Yao_Supplemental Fig10.
0
0.2
0.4
0.6
0.8
1
1.2
1 2 3 4 5 6
Rel
ativ
e cr
ossl
ink
effic
ienc
y CK shRNA
CTCF shRNA
p68 shRNA
ICR
H19 P endodermal enhancer
EE
Igf2 P1 P2
MAR3 DMR1
P4P3
1 2 3 6 anchor4 5
A
B
Yao_Supplemental Fig11.
