Crystal Structure of the Ribosome at 5.5 Å Resolution
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Crystal Structure of the Crystal Structure of the Ribosome at 5.5 Ribosome at 5.5 Å Å ResolutionResolution
Marat M. Yusupov,1* Gulnara Zh. Yusupova,1* Albion Baucom,1 Kate Lieberman,1 Thomas N. Earnest,2 J. H. D. Cate,3 Harry F. Noller1
1 Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA. 2 Berkeley Center for Structural Biology, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. 3 Whitehead Institute, Cambridge, MA 01242, USA.
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Describe the three-dimensional structure of the 70S ribosome of tDescribe the three-dimensional structure of the 70S ribosome of the completed he completed Thermus thermophilusThermus thermophilus containing mRNA and tRNA containing mRNA and tRNA bound to the A and E site at 5.5 bound to the A and E site at 5.5 ÅÅ resulution and to the A site at 7 resulution and to the A site at 7 ÅÅ resolution resolution..
Overrall structure of the 70s ribosome.Overrall structure of the 70s ribosome.• TheThe densityof tRNA densityof tRNA, bound to the P site of the 70S ribosome, at 5.5 , bound to the P site of the 70S ribosome, at 5.5 ÅÅ
resolution. resolution.
• Views of theViews of the stucture stucture of the of the T.T.thermophilusthermophilus 70S ribosome.70S ribosome.
• Secondary and tertiary structure of 16S, 23S,and 5S RNA.Secondary and tertiary structure of 16S, 23S,and 5S RNA.
Differences between the conformations of 70Sribosomes and isolatDifferences between the conformations of 70Sribosomes and isolated subunits.ed subunits.
StucturesStuctures of the of the intersubunit intersubunit bridges. bridges. tRNAtRNA-ribosome interactions.-ribosome interactions. Implications for the mechanism of translation.Implications for the mechanism of translation.
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Electron density of tRNAMetf bound to the P site of t
he 70S ribosome, at 5.5 Å resolution
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Subunits of prokaryotic ribosomes(70S)
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70S
核糖体的结构
A:30sA:30s
核糖体背面观
B:右面观(30S
亚基左,50S
右)
C
:从50S亚基背面观
D
:左面观
E
:上面观(50S上)
F
:50S
界面观(interface
,)
G
:300S
界面观(A旋转180
度)
[
灰色:23SRNA
;淡绿色:5SRNA
;兰绿色:16SRNA
;紫
红色:50S
蛋白质。A:金黄色;P
:桔黄色;E
:红色
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Secondary and tertiary structures of 16S, 23S, and 5S rRNAs (A:16SRNA; B:23S, 5SRNA; C:Three-dimentional fold of 16s RNA; D: C:Three-
dimentional fold of 23S and 5s RNA.)
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Conformational differences between rRNAs in 70S ribosomes and 30S and 50S subunits
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Intersubunit bridges
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Intersu
bu
nit b
ridges
(contin
ue
d)
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tRN
A-rib
osome in
teractions
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Two views of the A-tRNA anticodon stem loop bound
to its codon in the 30S subunit A site
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(A) Interaction of E-tRNA with the ribosome. (B) Secondary structures of 16S and 23S rRNA, showing mole
cular contacts with A-tRNA (gold), P-tRNA (orange), and E-tRNA (red)
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