Cryotome FE & FSE Operator Guide A78910100 Issue 4

7/26/2019 Cryotome FE & FSE Operator Guide A78910100 Issue 4

1/101

CryotomeFE & FSEOperator Guide - EnglishA78910100 Issue 4

WIP - Cryotome FSE - OP GUIDE - A78910100 - Iss4.indb 1 2/1/2011 10:46:53 AM


2/101

2004 Termo Fisher Scientific. All rights reserved.

Termo Shandon Limited is an ISO 9001 and ickI Accredited Company

Termo Fisher Scientific is the trading name of Termo Shandon Limited

Isceon is a trademark of Rhodia Chimie Corporation

All other trademarks are the property of Termo Fisher Scientific and its subsidiaries

Termo Fisher Scientific makes every endeavour to ensure that the information contained in its supportdocumentation is correct and clearly stated but does not accept responsibility for any errors or omissions.Te development of Termo products and services is continuous. Make sure that any published informationthat you use for reference is up to date and relates to the status of the product. If necessary, check with

Termo or your local Termo representative.

Tis manual may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted intoany electronic or machine readable form without prior written consent of Termo Fisher.

All information contained in this manual is proprietary and confidential, and the exclusive property ofTermo Fisher Scientific. Tis manual is protected by copyright and any reproduction is prohibited. Tismanual is for use only by the individuals to whom it has been made available by Termo Fisher Scientific.

Contact Addresses

Anatomical Pathology Anatomical Pathologyudor Rd, Manor Park, 4481 Campus Drive

Runcorn, Cheshire KalamazooWA7 1A, UK MI 49008, USA

el: + 44 (0)1928 534000 el: 1-800-522-7270

Fax: + 44 (0) 1928 534049 Fax: +1 269-372-2674

www.thermoscientific.com/pathology

Te Shandon Cryotome range meets the following CE Mark requirements:

In Vitro Diagnostic Directive 98/79/EC

Low Voltage Directive 73/23/EEC, as ammended by 93/68/EEC.

C US

Legal Information



3/101

A78910100 Shandon Cryotome Operator Guide 3

Contents

1 Welcome Introduction; Safety 5

2 Description Overview 9

3 Installation and Setting Up Procedure; Unpacking; Setting Up the Instrument;

Electrical requirements 11

4 ControlsDescription; Main control panel; connections 34

5 Operation Description, Safety Considerations; Precautions 60

6 Cleaning and MaintenanceIntroduction; Routine Cleaning and Maintenance

Instructions 67

7 Trouble Shooting Introduction; Instrument Function; Sectioning Suggestions 74

8 Specification and Accessories 81

9 Warranty Statement

Warranty Statement; Declaration of Conformity 86

Appendix A Transportation Instructions Instructions; Product Safety Declaration 88

Appendix B Approved Reagent List 94Appendix C Examples 95

Index 96



4/101

4 Shandon Cryotome Operator Guide A78910100

Te following symbols and conventions are used throughout thismanual and on the instrument.

HIS SYMBOL IS USED ON HE EQUIPMEN, ORIN A DOCUMEN, O WARN HA INSRUCIONSMUS BE FOLLOWED FOR SAFE AND CORRECOPERAION. IF HIS SYMBOL APPEARS ON HEINSRUMEN, ALWAYS REFER O HIS OPERAORGUIDE. s

HIS SYMBOL IS USED ON HE EQUIPMEN,OR IN A DOCUMEN, O WARN HA HEREMAY BE A BIOHAZARD ASSOCIAED WIH HE

INSRUMEN. ALWAYS AC WIH COMMONSENSE AND BE AWARE OF HE SAMPLES USED.AKE SUIABLE PRECAUIONS. s

HIS SYMBOL IS USED ON HE EQUIPMEN, ORIN A DOCUMEN, O WARN HA HERE AREPOENIAL PINCH POINS. IF HIS SYMBOLAPPEARS ON HE INSRUMEN, ALWAYS REFERO HE OPERAOR GUIDE. s

HIS SYMBOL IS USED ON HE EQUIPMEN,OR IN A DOCUMEN, O WARN HA HARMFULCHEMICALS ARE USED WIH HE INSRUMEN.REFER O HE MAERIAL SAFEY DAA SHEESFOR HE CHEMICALS USED. ALWAYS AC WIHCOMMON SENSE AND BE AWARE OF LOCALLABORAORY PROCEDURES. AKE SUIABLEPRECAUIONS. s

A warning is given in the document if there is a danger of personal

injury or damage to samples or equipment. s

Note Notes given more information about a job or instruction but donot form part of the instruction. s

!

WARNING

Symbols



5/101


Welcome

Chapter 1 Welcome

Welcome to Termo Fisher Scientific Shandon Cryotome FE andFSE Cryostats. Tey are designed for the routine sectioning of quick-frozen tissue specimens, for subsequent preparation and diagnosisby a pathologist. Te Shandon Cryotome series of cryostats areintended for use in pathology laboratories by appropriately trainedmedical laboratory technicians.

Designed and made with care, the instrument is safe to use, simpleto operate, and easy to maintain. Te Shandon Cryotome series ofcryostats conforms with IEC61010-1 and EN61326, the safety andEMC standards for laboratory instruments.

Tis Operator Guide gives instructions for the correct operationand use of the Shandon Cryotome FE and FSE. Instructions andinformation that applies only to a particular version of ShandonCryotome are marked [FE only] or [FSE only].

Termo products are designed for convenient and reliable operationand to accepted standards of safety. Te use of the ShandonCryotome does not entail any hazard if operated in accordancewith the instructions given in this manual. If the instrument isused in a manner not specified by Termo Fisher Scientific, thenthe protection provided by the instrument may be impaired. It isimportant for you to follow the following safety precautions:

Safety



6/101


Welcome

HE FOLLOWING PARAGRAPHS DEAILIMPORAN SAFEY INFORMAION. PLEASEREAD HIS SECION CAREFULLY. s

1. All users must read and understand the Operator Guide andonly operate the unit in accordance with the instructions. If theinstructions are not followed, then the protection provided bythe instrument may be impaired.

2. Do not modify the instrument - if unauthorised modificationsare carried out, the instrument may be made unsafe and thewarranty will be invalidated.

3. Potentially lethal voltages above 110V a.c. or 50V d.c. arepresent in the instrument.

4. Tis instrument has a protective earth system and must beproperly connected to a good earth (Ground) via the mainsinput supply,

5. Do not remove any panels or covers unless specifically instructedto do so.

6. It is important that normal standards of safety and goodlaboratory practises are employed. Always use of common senseand the best known practice when operating the instrument.

7. Sharp knives and blades are used in this instrument. Makesure you understand the correct methods for their installationand use.

8. Te centre of gravity position for the Shandon Cryotome FE orFSE is shown below:



7/101


Welcome

205mm353mm

566m

m

9. Te Shandon Cryotome weighs approximately 125 kilograms(275 lbs); get help to move it.

10. If the instrument has been used with materials that aretoxic or contaminated with pathogenic micro-organisms, followyour laboratory guidelines and the fumigation and cleaninginstructions given in Chapter 6 of this Operator Guide. TeProduct Safety Declaration (in Appendix A) must be completedif the instrument is to be returned to Termo or serviced by aTermo trained engineer.

11. Te instrument should be regularly cleaned as described inChapter 6 of this Operator Guide. Refer to Material Safety DataSheets when dealing with the reagents specified in Appendix B.

12. Do not use Shandon Cryotome for general refrigerationpurposes such as storage of specimens. Use a conventionalfreezer.

13. Use only Termo approved accessories or replacement partswith the Shandon Cryotome.



8/101


9/101


10/101


11/101


Installation and Setting Up

Te Shandon Cryotome is a precision instrument that must be

unpacked and installed with care.

Te maximum overall dimensions of the Shandon Cryotome FEand FSE cryostats are:

Width (with handle) 830 mm (33ins) Depth 745mm (29 ins) Height (working height) 1050mm (41 ins) (instrument height) 1205 mm (47 ins)

SHANDON CRYOOME FE AND FSEWEIGH APPROXIMAELY 125KG 275 LBS.ALWAYS GE HELP O SAFELY MOVE HEINSRUMEN WIHOU RISK OF INJURY. s

If the packaging has been damaged, check the condition of theinstrument. Contact your dealer if there is any damage.

Check that the detail on the label of the packaging corresponds withyour order.

Move the crate near to where the instrument is to be sited. Cutthe retaining straps and turn the three locking handles to releasethe packing case. Remove the outer case and lower the ramp. Readthe label on the rear of the instrument and check that the powerrequirements conform with your order.

Retrieve the accessories pack from on top of the pallet in front ofthe instrument. wo people are required to unload the instrumentfrom the packing case. Fit the transport handles to the back of theinstrument (the handles located in the foam packing at the back ofthe instrument).

Note Te handles should be left attached to make sure sufficientclearance is left behind the instrument to allow for ventilation and accessto the mains connector. s

Chapter 3 Installation and Setting Up



12/101


13/101



beyond 45, let it stand for 24 hours minimum before switchingon to allow compressor oil to settle. s

Te Shandon Cryotome can be moved for short distances - less than2 metres (6ft) without tilting the instrument (for example to pullthe instrument away from the wall). o do this, use the spannerprovided to turn the two front feet clockwise until the two front feetof the instrument are clear of the floor.

Push the instrument. When in position, turn the front feetcounter clockwise to lower them to the floor and adjust to level the

instrument.

Note Te feet can be left in the raised position, but there may be a slightmovement when sectioning. s

HE SHANDON CRYOOME IS HEAVY. O AVOIDCOLLISIONS, ALWAYS MAKE SURE HE ROUE ISCLEAR WHEN MOVING HE INSRUMEN. s

WARNING



14/101



InstallationMove the instrument to its permanent location. Tis must be leveland provide a 300 mm (12") clearance to the sides and rear of theinstrument for ventilation.

Note: Te transport handles at the rear of the instrument can be leftin place. s

Note Performance of the refrigeration system may be affected at ambienttemperatures above 25C (77F). s

Note Siting of the instrument on an uneven surface can have anadverse effect on the cooling system and the drainage of waste fluids fromthe chamber. Make sure that the unit is level. s

SIE HE INSRUMEN IN A WELL VENILAED AREAAWAY FROM DOWNDRAUGHS. DO NO SIE HEINSRUMEN NEAR O SOURCES OF DOWNDRAUGHSSUCH AS WINDOWS OR HEAING / VENILAIONDUCS AS HE EFFICIENCY OF HE CHAMBER CAN BE

AFFECED. s

Use the spanner provided to turn the height adjustment knobs

counter-clockwise until the feet of the instrument are on the floorand the unit is stable and level.

300mm



15/101



Raise the front edge of the top cover, remove the transit packingfrom the top of the sliding windows, then lower the cover backinto position. Push open the sliding window to gain access to therefrigerated chamber.

Note Make sure the window is closed before the top cover is lowered intoposition so that the cable is not caught up. s



16/101



Electrical

requirements

Make sure that the voltage of the mains supply corresponds with thevoltage rating on the rating plate on the back of the instrument. Temains supply requirements are listed in Chapter 8.

Note Te ~ symbol on the rating plate indicates that the instrumentoperates on an alternating current supply (a.c.) s

Make sure that the I / O power switch at the rear of the instrumentis switched off (Oside of the switch pushed inward).

Instruments are supplied with power cords with moulded plugssuitable for many countries. If another plug is required, a technicallycompetent person should use the wiring convention shown in thefollowing table to replace the existing moulded plug with a suitablyrated, and, where appropriate, fused plug:

European cable US Cable Terminal

Brown Black Live (L or L2)

Blue White Neutral (N or L1)

Green / Yellow Green Earth - E, ground

Mains inlet socket On /off switch Rating plate



17/101



HE SHANDON CRYOOME MUS BEPROECIVELY EARHED. MAKE SURE HA HEINSRUMEN IS PLUGGED INO A PROPERLYEARHED GROUNDED MAINS SUPPLY. HESHANDON CRYOOME MUS BE PROECIVELYEARHED. MAKE SURE HA HE INSRUMENIS PLUGGED INO A PROPERLY EARHEDGROUNDED MAINS SUPPLY. s

I MUS BE POSSIBLE O INERRUP HE MAINSSUPPLY A SOURCE BY REMOVING HE PLUGFROM HE MAINS SUPPLY SOCKE. s

Select the appropriate mains lead from those supplied and plugit into the mains inlet socket in the rear panel of the instrument.Connect the mains supply cable to the local power supply outlet.

A RANSI BRACKE IS AACHED O INERNALSRUCURES O PROEC HE INSRUMEN

DURING RANSPOR. I IS ESSENIAL HA IIS REMOVED BEFORE AN AEMP IS MADE OOPERAE HE INSRUMEN. s

Open the window to access the chamber. Gradually loosen the twocap screws on the verticle part of the bracket (by no more than 2 fullturns). Loosen the cap screw on the base if the transit bracket.

Lift the Shandon Cryotome head out of the bracket to its topposition. Gently slide the bracket towards you along the knifeholder base and remove it.

Note Store the transit bracket for future use - it is needed when theShandon Cryotome has to be transported. s

!

Transit Bracket

!

!



18/101


19/101



Carefully locate the handwheel into the hole at the top of the righthand side. Te handwheel is supplied in the accessory pack.

Note If the specimen head is at the bottom of the stroke, the handwheelhandle should also be at the bottom. s

Make sure that the specimen head is at the top or bottom andturn the handwheel until the handle is at the top or the bottom tomatch. Make sure that the locating pin of the handwheel fits intothe key-way of the drive shaft.

Place the spring washer and the flat washer (in that order) onto thehandwheel bolt. Insert the bolt into the recess on the handwheel

and use the Allen key to tighten it (turn it clockwise). Fit thepush-in plug. Rotate the handwheel and check that movement issmooth and produces a corresponding movement of the specimenhead.

Te lever next to the handwheel handle is used to lock thehandwheel in position. Te handwheel can be locked in one ofthree positions - 12 o'clock, 3 o'clock and 6 o'clock, by pushingthe lever away from you.

Fitting theHandwheel

Handwheel bolt Push-in plug

Flat washer Spring washer



20/101



urn the handwheel until the handle is used to lock the handwheelin position then push the lever of the lock away from you. Checkthat the lock operates and that the handwheel cannot rotate. Checkthat the specimen head is at the top of its travel.

Move the lock lever towards you and check that the handwheelrotates smoothly and that the specimen head moves up and down.Check also that the lock operates at the 3 o'clock and 6 o'clockpositions.

Read and understand the following warnings:

PRACICE GOOD BIOLOGICAL SAFEYPROCEDURES WHENEVER HE SHANDONCRYOOME IS USED. s

MAKE SURE HA HE WASE BOLE IS FILLEDWIH SUFFICIEN 10% FORMALIN SOLUIONO COVER HE OULE PIPE. REFER O HEMAERIAL SAFEY DAA SHEE FOR FORMALIN

AND FORMALDEHYDE. s

DO NO USE HE SHANDON CRYOOME FORGENERAL LABORAORY REFRIGERAIONPURPOSES. s

Fumigate regularly to ensure decontamination of the refrigerationchamber and accessories. s

Use cut resistant gloves when handling knives and blades. s

Use the correct type of knife or blade appropriate for the knifeholder. s

Make sure that the knife guard is correctly installed. s

Preparing theinstrument for use

WARNING

WARNING

WARNING

WARNING



21/101



IF YOU KNOW HA A SPECIFIC VIRUS ORBACERIA IS LIKELY O BE PRESEN IN HESPECIMEN, MAKE SURE HA YOU ARE AWAREOF A SUIABLE DECONAMINAN BEFORE YOUINRODUCE HE SPECIMEN INO HEREFRIGERAED CHAMBER. s

ALWAYS MAKE SURE HA HE WINDOW ISCLOSED BEFORE ANY SECIONS ARE CU. s

O AVOID INJURY, DO NO MOVE HE SPECIMENHEAD WHEN YOUR HANDS ARE IN HE CHAMBER.s

Remove the waste bottle. Use approximately 200ml of a 10%solution of formalin (4% formaldehyde solution) to fill the wastebottle sufficient to cover the outlet tube.

Refit the waste bottle - make sure that the outlet tube fits throughthe hole in the lid and the end of the tube is below the level of theformalin.

REFER O MAERIAL SAFEY DAA SHEE MSDSFOR FORMALIN AND FORMALDEHYDE. s

KEEP SUFFICIEN FORMALIN SOLUION OCOVER HE END OF HE OULE UBE IN HEWASE BOLE A ALL IMES O PREVEN HERELEASE OF AEROSOLS OR CONAMINANSDURING USE. s



22/101



Setting up the Shandon Cryotome for routine operation involvesthe installation and adjustment of one or more of the following:

i Knife holderii Knife guardiii Disposable blade or solid knifeiv Cutting anglevii Anti-roll plate

Te disposable blade holder comprises three sections - a base, middleand top. Te blade holder assembly is fitted to a common supportplate attached to the base of the chamber.

Te base section allows movement towards and away from thespecimen holder. Use the black lever (position clamp) on the lefthand side to clamp the blade holder in position.

Routine Operation

Disposable BladeHolder

Position clamp

BLACK



23/101



Te middle section can be rotated on the base section to achieve theoptimum cutting angle. Use the red level (angle clamp) on the righthand side to clamp the blade holder at the correct cutting angle.

Te top section can be moved laterally along the middle sectionto use the entire length of the blade. Use the blue lever (bladeposition clamp) on the left hand side to clamp the top section inposition. wo different top stages are available for low and highprofile blades.

Pull the red angle clamp towards you. Slide the middle section ontothe base section of the knife holder, so that the base section plungerlocates in the middle section slot. Line up the two sections and pushthe red angle clamp to lock them in position.

Pull the blue blade position clamp towards you. Line up thedovetails on the top section and the middle section and slide thetwo parts together. When the top section is central, push the blueblade position clamp away from you to tighten it.

Note When fitting the top section to the middle section, lift the end ofthe top section slightly when sliding it along the middle section.s

Angle clamp

RED

Blade position clamp

BLUE

Assembling thedisposable blade holder



24/101



Slot Top section

Angle clamp - RED

Plunger

Base section

Position clamp - BLACK Blade position clamp - BLUE Middle section

e blade holder is now assembled.

Note Make sure that all the assembly slots, dovetails and plungers arefree from debris. If debris is present, the assembled blade holder may not

provide the required stability. s

Once assembled, the disposable blade holder can be fitted into theShandon Cryotome FE and FSE chamber.

ALWAYS LOCK HE HANDWHEEL O PREVENHE SPECIMEN HEAD MOVING WHEN WORKINGNEAR HE KNIFE HOLDER. s

Pull the black position clamp on the left hand side of the base sectionfully toward you.

Insert the plunger on the underside of the base section into thecircular hole at the end of the support plate slot (the support plate islocated in the base of the chamber).

Fitting the disposableblade holder



25/101



Slide the blade holder assembly along the support plate to the

required position and push away the black position clamp to lockit in place.

o adjust the angle of the knife holder, pull the red angle clamp fullytoward you. Adjust the angle of the knife holder until the '0' of theangle scales is approximately in line with the marker on the basesection, then push the red angle clamp away from you to lock it.Tis gives a cutting angle of 22.

Locating plunger

Support plate slot

Position clamp

BLACK

Adjusting the disposableblade holder

Angle clampRED

Scale



26/101



Note Te knife holder is adjusted to its precise operating angle after theblade or knife is installed. s

Note Te blade type used may affect the optimum blade angle. ForKai MX35 blades, start to section at an angle of 3on the scale andmake small adjustments to achieve optimum performance. s

Be aware of the dangers of handling microtome blades.

ALWAYS USE CU RESISAN GLOVES O AVOIDINJURY WHEN HANDLING DISPOSABLE BLADES.USE HE DISPENSER WHEN FIING A NEW

BLADE. s

Use the anti-roll plate handle to swing the anti-roll plate away fromthe blade clamp.

Pull the black blade clamp lever towards you to release the bladeclamp. Push the end of the blade to be installed part way out of thedispenser and feed it behind either end of the blade clamp.

Slide the blade to the right until it is locked symmetrically in thetop stage behind the blade clamp.

Note Te blade must be located symmetrically for correct operation ofthe anti-roll plate. s

Fitting a disposable blade

Blade fitted behind blade clamp

Anti-roll plate

handle

Anti-roll

plate

Blade clamp lever - BLACK



27/101


28/101


29/101



Te specimen heads are designed to accept a Shandon Cryocassettethat already has a frozen specimen mounted.

Te Shandon Cryotome FE can be fitted with either a fixed or a fineorientation head.

Te Shandon Cryotome FSE is only fitted with a fine orientationhead for use with independent specimen cooling.

Te frozen specimen on the Cryocassette is held in the specimenhead by a Quick Release Cryocassette Clamp. Te clamp lever at theleft hand side of the Specimen head operates a moveable jaw at thebottom of the head that holds the Cryocassette firmly in position.

o install a cryocassette in the Quick Release Clamp:

i Pull the left hand clamp lever forward

ii Fit the cryocassette under the two fixed jaws as shown

iii Push the clamp lever back.

Note Te cryocassette handle (supplied) may be fitted to thecryocassette to aid manipulation and fitting to the specimen head. s

Fixed head (FE) Fine orientation head (FE)

Fixed jaws Fixed jaws

Quick release cryocassette clamp lever:

Pull to release, push to clamp (shown in released position

Specimen Head



30/101


31/101



he following accessories can be used with the Shandon Cryotomeand FE and FSE.

Fit the narrow shelf (with side lip) to the right hand side of thechamber against the side wall if it is required for tool storage.

Fit the flat shelf next to the cryobar for storage of specimens atchamber temperature.

A range of cryocassette sizes and styles are available.

A removable / lockable handle can be used to handle the

cryocassettes.

Tese are clear strips with numbered rings designed to fit on thecryobar or shelves to locate and identify cryocassettes that are beingfrozen or stored before sectioning.

Te heat extractors fit in the slots beside the cryobar to makespecimen embedding and preparation easier. Tree different weightsare available.

If the cryobar is at a lower temperature than the chamber, it willattract frost. o keep the cryobar surface clear from frost and debris,place the cryobar cover on it whenever it is not being used to freezespecimens.

o adjust the foot rest to suit individual requirements, unscrew andremove the knobs that secure the footrest to the instrument.

DO NO SAND ON HE FOO RES. s

Height

positions

Foot rest knobs

Accessories

Shelves

Cryocassettes

Cryocassette handle

Cryocassette identifier

Heat extractors

Cryobar Cover

Foot rest



32/101



Move the foot rest to one of the other positions, and line up thefootrest with the appropriate hole. Insert the knob and tighten itslightly to allow you to attach the other side of the foot rest. ightenboth knobs.

Note Te footrest does not have to be fitted. Remove it if this is moreconvenient. s

A switch is provided for connection to a remote alarm circuit. Teswitch operates if:

i A power fail occurs

ii An error occurs from which the instrument cannotautomatically recover

iii Te instrument malfunctions


Remote alarm relay connections:

NO C NC Remote alarm fuse

Mains inlet socket On /off switch

Remote Alarm



33/101



Te switch is rated for operation at 3A 250Va.c. / 24Vd.c. anddisconnected in circuit with a named three pin socket at the rear ofthe Shandon Cryotome FE and FSE. Te remote alarm facility isnot a powered output.

A remote alarm connector is supplied, plugged into the remote alarmsocket on the rear of the instrument. Use this to make connectionsto a remote alarm sounder.

If the remote alarm has been activated, the middle (commonterminal - C) and right hand (NC) pins are connected as markedabove the connector. In normal operation, the middle (commonterminal - C) and left hand (NO) pins are connected.

Only a technically competent person may change the remote alarmfuse or connect an external circuit to the remote alarm socket. Teexternal circuit must comply with the requirements of IEC61010-1and IEC950.



34/101


Controls

Te Shandon Cryotome is controlled via a touch screen displaypanel. Te images are displayed as black on a white background. Ifan item is selected, this is inverted to white on a black background.

o select a button, touch the relevant image displayed on the screen.

Use your fingertips (not fingernails or sharp instruments). Do notpress too hard.

DO NO PRESS IN MORE HAN ONE PLACE A A IME A FALSE KEY PRESS MAY OCCUR. s

Note Tere is no feel when the image is pressed, but there is anaudible beep when a valid button is pressed. If any other area of the

screen is pressed, there is no beep. s

When the display backlight is dim and the chamber light is off,the instrument is in standby mode. If the user touches the screensurface, opens the window or operates any other control, theShandon Cryotome will sense that a user is at the instrument andwill exit from standby mode. Te display backlight and chamberlight will then come on.

If the instrument is not used for 15 minutes, then it will revert tostandby mode.

Chapter 4 Controls

Not Selected Selected



35/101


36/101


Controls

Te Shandon Cryotome is controlled by pressing buttons on thetouch screen display panel. Tis section describes each screen andthe buttons that appear.

Te buttons are described below:

Tis displays the selected section thickness in mm. Normallywhen the Main Screen is entered, the MICRONS button will behighlighted.

Tis displays the actual temperature of the cryobar in C.

Screen Controls

Main Screen

Selector Buttons

Microns

Cryobar



37/101


Controls

Note When the cryobar is in boost mode, the CRYOBAR button willhave an asterix displayed and the text between the adjustment buttonswill display the amount of time remaining for the boost cycle (as shown

below). s

Tis displays the actual temperature of the chamber in C.

Tis displays the actual temperature of the specimen head in C

[FSE only].

Note When the Cryotome is in the Unload sequence, the SPECIMENbutton will have an asterix displayed and the text between the adjustmentbuttons will display the amount of time remaining for the cycle. s

Chamber

Specimen



38/101


Controls

Te OPIONS and UNLOCK buttons share the same screenlocation, but are displayed in different conditions.

When the instrument is unlocked, the OPIONS button is

displayed. Press and hold the OPIONSfor approximately1 second to access the Options Screen (page ).

When the instrument is locked, the UNLOCK button isdisplayed instead of the OPIONS button. Te action ofthe UNLOCKbutton depends on the security mode. (Seethe Security Setup Screen - page ).

Tese buttons adjust the value of the set point associated with thehighlighted selector button

Micron increments 1-20mm in 1mm steps

20-60mm in 5mm steps

emperature increments 1C steps

Minimum emperature -60C (settable temperature)

Workable temperatures:

(specified for an ambient temperature + 25C)

Cryochamber 0 to -35C

Options / Unlock

Adjuster Control Arrows

Up and Down



39/101


Controls

Te display located between the Up and Down arrows shows the setpoint associated with the highlighted selector button.

If a temperature setpoint of


40/101


Controls

Te ERROR button displays a short description of the current error.It flashes to highlight the error.

Press ERRORto display the Error Screen that describes the current

error.

Press EXI to clear the Error Screen and return to the MainScreen.

Options Screen



41/101


Controls

Te buttons on the Options Screen are described below:

Either the LOCKor the UNLOCK button is displayed in the samescreen location.

Te LOCK button is only displayed when the instrument isunlocked. Press LOCKto lock the window and activate the securitymode selected (pg ).

Te UNLOCK button is only displayed when the window islocked and the security mode is off. Press UNLOCKto unlock thewindow.

o carry out an immediate defrost cycle, press DEFROS to displaythe Defrost Screen from where the required defrost program can beselected ( page )

Press FUMIGAE to display the fumigation screen (page )

Note Use the UNLOAD SPECIMEN feature to warm the head toabove 0C to enable the cryocassette to be removed from the specimenhead, or the specimen from the cryocassette. At the end of the unload orwhen CANCEL UNLOAD is pressed, the specimen head will return toits set point. s

Either the UNLOAD SPECIMEN or the CANCEL UNLOADbutton is displayed in the same screen location.

Lock / Unlock

Defrost

Fumigate

Unload specimen

[FSE ONLY]



42/101


Controls

Press UNLOAD SPECIMEN (or press SPECIMEN on the mainscreen twice) to start a specimen unloaded sequence. Te MainScreen will be displayed and will show that unloading is in progress- the SPECIMEN button will have an asterix displayed and the text

between the adjustment buttons will show the countdown time of5 minutes:

o cancel the unload sequence and return to the Options Screen,either press CANCEL UNLOAD or press the SPECIMEN buttonon the Main Screen when it is selected.

Either the CRYOBAR BOOS or the CANCEL BOOS button isdisplayed in the same screen location.

o start a cryobar boost sequence either press CRYOBAR BOOS orpress the CRYOBAR button on the Main Screen when it is alreadyselected.

Te Main Screen will be displayed and will show that the boostsequence is active - the CRYOBAR button will have an asterixdisplayed and the text between the adjustment buttons will showthe amount of time remaining for the boost cycle:

Press CANCEL BOOS in the Options Screen to stop the boostscycle and return to the Main Screen.

Cryobar boostCancel boost



43/101


44/101


Controls

o enable the cryobar:

1. From the Main Screen, choose OPIONS, CRYOBARENABLE

Note If the cryobar was disabled by method 1 above, thecryobar will cool down to a default setpoint of -30C. s

Note If the cryobar was disabled by method 2 above,the cryobar will return to the last setpoint used.For example, if the cryobar was set at -40C before it wasdisabled, the cryobar will go to -40C again. s

2. From the Main Screen, press . Te set temperature

will then decrease from C,

Press SEUP MENU to display the Setup Screen (see below)

Press EXI to leave the Options Screen and return to the MainScreen (page 36)

Press the required function key to display the Setup Screen for thatfunction (pages 45 - 53)

Setup Menu

Exit

Setup Screen



45/101


Controls

Note If any change is made to the Setup screens then EXI is re-placed by SE or SELEC. s

Press EXI to leave the Setup Screen and return to the MainScreen (page 36).

The time and day display shows the days of the

week and the time (in either 12 hour or 24 hour

format).Te and buttons above and below each part of the imeand Day display will increase or decrease that value by 1 - forexample:

Button Change in time and day display

button above the day Wednesday, Thursday, Friday, Saturday...

button below the day Monday, Sunday, Saturday...

button above the hour Increments the hour display by 1 hour ( including changing the am/

Shandon pm setting if 12-hour format is selected) - 05, 06, 07

button below the hour Decrements the hour display by 1 hour - 03,02,01

button above the minute Increments the minute display by 1 minute- 13,14, 15

button above the minute Decrements the minute display by 1 minute - 11, 10, 09

Exit

Set Clock Screen

Time and day display



46/101


Controls

Either the 12HOUR or the 24HOUR button is displayed in thesame screen location.

Te24HOUR button is only displayed when the clock is in 12-hourmode. In 12-hour mode, the display will show am or ShandonShandon pm.

Press24HOUR to change the time display to 24 hour mode andvice versa.

Press SEto exit to the Setup Screen and save changes made to thetime and day.

Note Te day and time are always displayed at the top of the MainScreen. s

12 Hour

24 Hour

Set



47/101


48/101


Controls

Te buttons on the Security Setup Screen are used to select therelevant security mode. Te security mode determines how youaccess the instrument when it has been locked. Te highlightedbutton shows the current security mode.

Te security mode is activated when the LOCKkey in the optionsscreen is pressed (page )

Note Te EXI button will change to SELEC if you press any of thesecurity mode buttons. s

Te three different security modes are described below:

When the security mode is activated, the window will be locked andthe controls disabled. Te screen options remain active.

o release the controls, unlock the screen from the Options Screen.

Tis is the default option. It provides basic security to preventinadvertent button presses, for example when cleaning the screen.

When the hold key mode is activated, the window will be locked,and the screen options and controls disabled.

When the screen or a control is touched or moved, the OUCHAND HOLD O UNLOCKbutton is displayed.

Press and hold this button for approximately 3 seconds to unlockthe instrument.

Security ModeActivation

Security Setup Screen

Security Modes

Security Off

Hold key to unlock



49/101


Controls

Tis provides full security where an access code number is requiredto unlock the instrument.

When the access code mode is activated, the window will be locked,

and the screen options and controls disabled.

When then screen or a control is touched or moved, the EnterUnlock Code Screen is displayed (page 52). Te current access codemust be entered before the screen or controls can be used.

Te section describes how to select each security mode. o accessthe Security Mode Screen, from the Main Screen, select OPIONS,SEUP andSECURIY SEUP.

Te current security mode will be highlighted.

o set the security off mode:

If the instrument is already in the security off mode (ie the

SECURIY OFF button is highlighted), the selection can beconfirmed by pressing the SECURIY OFF button. Press SELECto return to the Setup Screen (page 44).

If the instrument is in the hold key mode (ie the HOLD KEY OUNLOCKbutton is highlighted), the selection can be confirmed bypressing the SECURIY OFFbutton. Press SELECto return tothe Setup Screen (page 44).

If the instrument is in the access code mode (ie theACCESS CODEbutton is highlighted), choose the security off mode by pressing

SECURIY OFFbutton. Press SELECto confirm this selection.Te Enter Current Code Screen will be displayed (page ) Inputthe current access code and press ENER to return to the SetupScreen (page 44).

Note Te access code will be cleared. s

Access Code

Security ModeSelection

Security Off



50/101


51/101


Controls

CODEbutton. Press SELEC to confirm the selection. Te EnterNew Code Screen will be displayed (page 52).

Input the new access code and press ENER. Te Re-enter New

Code Screen will be displayed (page 52). Input the new access codeand press ENERto return to the Setup Screen (page 44 ).

Tis selection applies to a series of four screens that all look similar.Te screen names are displayed at the top of the screen (in this caseEnter New Code). Te four screen are:

Enter New CodeRe-enter New CodeEnter Unlock CodeEnter Current Code

Te buttons on all the Access Code Screens are described below:

Press the relevant number button to input the requires Access Code.Te numbers will be displayed as * in the code display at the topright of the screen.

Press ENER to confirm the 4 digit code that was entered.

PressCANCEL to reset all the entered digits as dashes and to exitback to the Security Setup Screen.

Te Access Code Screens are described below:

Access code screens

Number Buttons

Enter

Cancel



52/101


Controls

When this screen is displayed, enter a new access code. Press ENERto submit the code.

he screen will now change to the Re-Enter New Code Screen.

Tis screen checks that you have entered the code correctly. ype inthe same code that you entered in the Enter New Code Screen andpress ENER.

If this code agrees with the first code entered then the code numberis valid and should be used whenever requested.

Tis screen is displayed when the instrument has been locked anda control is moved or touched. Enter the current valid access codefollowed by ENER to unlock the instrument.

Tis screen is displayed when the access code security mode is selected,and a button for another option is pressed. Enter the current validaccess code and press ENERto access different screens.

Note o clear the Access Code, change to another security mode(pages 48-51 ). s

Use this screen to set the start item for the daily automatic defrostcycle (which only defrosts the cooling fins in the back of the chamber).Te buttons on the Defrost Setup Screen are described below:

Note If the clock is set to 24-hour mode, then the defrost time willalso be in 24 hour mode; if the clock is set to 12 hour mode, then thedefrost time will also be in 12 hour mode. s

Enter New Code

Re-enter New Code

Enter Unlock Code

Enter Current Code

Defrost setup screen



53/101


Controls

Te main part of the Defrost Setup Screen is taken up with theINCREMEN and DECREMEN buttons and theime Display.

Te time Display shows the defrost start time in hours andminutes.

Te and buttons above and below each part of the imeDisplay will increase or decrease that value by 1 - for example:

Button Change in time and day display

button above the hour Increments the hour display by 1 hourNote When the hour display reachesmaximum, the hour display will revert to 0.

button below the hour Decrements the hour display by 1 hour.

button above the minute Increments the minute display by 1 minuteNote When the minutes display reaches 60,the hour display will NO increment.

button above the minute Decrements the minute display by 1 minute.

Press SEto confirm the defrost time and return to the Setup Screen(page 44)

Note During the defrost cycle, the chamber temperature will rise.However the cryobar will not be affected if it is enabled. s

Set

Time Display

Increment Button

Decrement Button



54/101


55/101


56/101


57/101


58/101


Controls

I IS IMPORAN HA ALL HE KNIFEHOLDER SECIONS ARE SEPARAED WHENCLEANING. MAKE SURE HA ALL SURFACES ARECOMPLEELY DRY BEFORE REASSEMBLING ANDREFIING INO HE CHAMBER. s

Press EXIto enable the instrument to return to normal operationwith the previously set temperatures.



59/101


60/101


Operation

Operation

When the instrument is not in use, lock the Handwheel and keep theinstrument switched on, with the sliding window closed and locked,and the refrigerated chamber at the normal operating temperature.

When the lock option is selected, the following things occur:

the touch screen control panel is disabled (dependant on thesecurity mode)

the window is locked

the chamber light is turned off

the display is dimmed

SURFACES INSIDE HE CHAMBER MAY BE EIHERVERY HO OR VERY COLD AND MAY CAUSEINJURY IF OUCHED WIH BARE HANDS. s

BE AWARE HA ISSUE SAMPLES MAY POSE ABIOHAZARD. AKE SUIABLE PRECAUIONS. s

BE AWARE OF POENIAL BIOHAZARDS ANDFORMALIN CONAINED IN HE WASE BOLE.DISPOSE OF CORRECLY IN ACCORDANCE WIHLOCAL PROCEDURES. s

BE AWARE HA FORMALDEHYDE SOLUION IS

USED DURING FUMIGAION. ALWAYS REFER OMAERIAL SAFEY DAA SHEE MSDS.s

O AVOID INJURY, LOCK HE HANDWHEEL ANDDO NO MOVE HE SPECIMEN HEAD WHENYOUR HANDS ARE IN HE CHAMBER.s

Chapter 5

!



61/101


62/101


Operation

HE CRYOBAR IS EXREMELY COLD AND CANCAUSE FROSBIE. DO NO OUCH I WIHBARE HANDS. s

(FSE version only) Use the table in Appendix C as a guide to selectthe temperature for the specimen to be cut according to the tissuetype.

(FSE version only) o set the specimen head temperature, pressSPECIMENto select it and then use or to set the requiredtemperature.

(FSE version and FSE versions) Press CHAMBER and use orto set the chamber temperature to the required temperature

(normally between -20C and -25C, but should be at least as low asthe specimen temperature guidelines listed in Appendix C).

Make sure you have a cooled blade ready for use.

Tere are several methods of embedding a specimen for work ona Shandon Cryotome. Te following instructions describe onesuggested method.

ake a clean Cryocassette that has been held a room temperature.Apply a layer of specimen embedding medium, such as Cryomatrixor Cryochrome, to the grooved side of the Cryocassette and placethe Cryocassette on the cryobar.

When the embedding medium starts to freeze, place the specimendirectly from the cutting - up board onto the embedding mediumand press down lightly to eliminate any entrapped air. Add extramountant to support the specimen if required.

o give a flat and parallel surface, and quicker freezing, lower thecooled heat sink onto the specimen. Allow the specimen andmountant to freeze until both are opaque and firm.

!



63/101


Operation

O AVOID HE POSSIBILIY OF CROSSCONAMINAION:

1. MAKE SURE HA HE CRYOBAR AND HEAEXRACOR ARE COLD ENOUGH

2. DO NO USE OO MUCH PRESSURE WHENAPPLYING HE HEA EXRACOR

3. MAKE SURE HE CRYOCASSEES AND HEASINKS ARE CLEAN BEFORE USE. s

Note Do not use excessive mountant or get it onto the sides or back ofthe Cryocassette. s

Note CRYOBAR BOOS continues for 25 minutes after cryobarboost is initially enabled. Tis time can be adjusted in 5 minute stepsby pressing or .

Tere are two ways to switch off the cryobar boost during this period:

1 Press until the time displayed between the Adjuster ControlArrows is 15 seconds. Te instrument will then count down until theboost ends.

2 Select OPIONS followed by CANCEL BOOS.

An audible warning will be sounded when the boost cycle is about toend. s

urn the handle of the handwheel to the 12 oclock position, andapply the break.

Fit the cryocassette handle (supplied) to the cryocassette and lock itin place by sliding the locking tab towards the cryocassette. ransferthe Cryocassette, complete with frozen specimen, from the cryobarto the specimen head and secure it in position with the specimenclamp (see page 29). Te handle can now be removed, if required,

!



64/101


65/101


Operation

Use the brush to discard any unwanted sections - sweep them awayto the sides or rear. DO NO BRUSH AGAINS HE OP OFHE BLADE.

Pick up the required selections on a slide.

Continue to turn the handwheel to cut more sections. Te specimenautomatically advances by the section thickness before the start ofeach cutting stroke and is automatically retracted by 40 micronsduring the return stroke.

When the last section has been removed, fit the knife guard andremove the cryocassette from the clamp using the cryocassette

handle.

FI HE KNIFE GUARD OR REMOVE HE BLADE WHENNO CUING SECIONS. s

(FSE only) o help remove the cassette and/or specimen from thecassette, either press SPECIMEN twice or go to the Options Screenand select UNLOAD SPECIMEN. Te specimen head will warmupto +2c to allow the cassette and/or specimen to be removed. It

will then return to its set temperature. Press SPECIMEN again tostop UNLOAD(or CANCEL UNLOADfrom the Options Screen)(page 40).

Te specimen set temperature will be replaced byUNLDduring thisperiod. Te display will flash and an audible warning given whenthe temperature reaches +2C.

If the specimen is to be stored frozen, protect the cut surface withShandon Cryotome, wrap the specimen in metal foil before putting

it into a freezer. Do not store it in the refrigerated chamber of theShandon Cryotome.

If the specimen is to be fixed, allow it to thaw before placing itin a suitable fixative; otherwise discard it in accordance with localregulations and procedures. Brush away any debris that has collectedaround the cassette clamp or head.

Removing

Specimens



66/101


67/101


Cleaning and Maintenance

Chapter 6 Cleaning and Maintenance

It is important that decontamination and cleaning of the instrumentbecomes an automatic routine - especially if the source of material isunknown. It is recommended that a log is kept which lists:

i Material Cutii Degree of Riskiii Decontamination Performediv Name of Userv Department.

Cleaning is a part of good laboratory housekeeping practice. Externalcleaning requirements are straightforward. Te refrigerated chambershould only be washed and cleaned if required after the chamber isthoroughly decontaminated by fumigation. Te instrument shouldbe inspected for obvious damage whenever it is cleaned. If anydamage is found, contact your Termo supplier.

Routine maintenance by the operator does not require the removalof any panels or fixtures. Precision maintenance and adjustmentshould only be performed by Termo trained service personnel and

it is recommended that a Maintenance Contract is obtained fromour Service Department.

IF HAZARDOUS MAERIAL IS SPLI ON, ORINSIDE, HE INSRUMEN, HE USER SHOULDCARRY OU HE APPROPRIAEDECONAMINAION (see World Health OrganisationLaboratory Biosafety Manual.) s

CLEANING OR DECONAMINAION MEHODS,OHER HAN HOSE RECOMMENDED IN HEOPERAOR GUIDE, SHOULD BE CHECKEDWIH A HERMO AGEN O ENSURE HA HEPROPOSED MEHOD WILL NO DAMAGE HEEQUIPMEN. s



68/101


69/101



Carefully load the heating trough in the refrigerated chamberwith 3ml of formaldehyde solution. Close the window and pressOPIONS.

o start the fumigation, press FUMIGAEin the Options Screen.Te Fumigation Screen will display instructions. Press SAR tobegin the fumigation cycle.

If the window is not closed, the screen will show an error and willprompt you to close it.

Do not use any ammonia based reagent to neutraliseformaldehyde. s

Note For safety, the fumigation cycle cannot take place if the windowis not closed and locked. s

Carefully remove the shelves, debris tray, covers, knife holder andany debris.

Wash the shelves, debris tray and covers in running hot waterinitially to remove the debris. Wash, dry thoroughly and replace in

the Shandon Cryotome.

Follow the instructions below to clean the knife holder.

If the chamber is cold, wash it with alcohol to remove debris, andmake sure the outlet tube in the bottom of the chamber is free fromdebris.

Do not use scouring powder, acidic solutions or harsh detergentsor you will degrade the brushed finish. s

Press EXI and the chamber will return to the previously settemperature.

WARNING

End of fumigation

WARNING



70/101


71/101



Te Cryobar Defrost cycle will defrost the cryobar only and will lastfor approximately 5 minutes.

HE CRYOBAR MAY REACH EMPERAURESOF MORE HAN +50C DURING HE DEFROSCYCLE.

1. REMOVE HE SPECIMENS FROM HE CRYOBARBEFORE DEFROSING

2. DO NO OUCH HE CRYOBAR DURINGDEFROSING. s

o completely defrost the instrument, open the window and switchoff both compressors (by raising the set points to +1C or switchingoff the instrument).

Te waste bottle collects water from the cooling fins. It also collectsdebris when the refrigerated chamber is washed down so the contentsmust be disposed of in an appropriate manner.

MAKE SURE ALL SURFACES AND PARS AREHOROUGHLY DRY BEFORE HE COMPRESSORS

ARE RESARED. s

ALWAYS KEEP A LEAS 200 mlOF 10% FORMALINSOLUION 4% FORMALDEHYDE SOLUION ORSIMILAR DECONAMINAN IN HE WASEBOLE. MAKE SURE HA HE END OF HEDRAIN UBE IS KEP SUBMERGED. s



72/101



No maintenance is required to be carried out by the user.

If the fluorescent tube fails, either follow the instructions below or

call the Termo Service Department.

SWICH OFF HE INSRUMEN A HE MAINSAND REMOVE HE PLUG FROM HE WALLSOCKE BEFORE PROCEEDING WIH ANYMAINENANCE.s

Lift up the top cover.

DO NO OUCH ANY WIRES, CONNECORS ORCOVERS INSIDE HE INSRUMEN. s

Grip the fluorescent tube firmly, rotate it be a quarter turn and pullit towards you to slide it from the end connectors.

Hold the replacement tube firmly at the ends and align the connectorpins with the slots at each end of the fitting.

Carefully push the tube into place and rotate it by a quarter turn.

Te mains input supply feeds the Shandon Cryotome via a tripdevice that interrupts the electrical supply to the instrument if aninternal fault occurs. Te trip replaces the function of a fuse.

When the electrical protection device trips, the mains switch goes toits O position (OFF) and the instrument becomes inoperative.

If the protection circuit operates, this will normally indicate acompressor problem. Check that there is adequate space aroundthe outside of the unit for cooling air and that the inlet grill on theright hand side is free of obstructions and dust. Allow 1 hour for thecompressor to cool before attempting to reset the trip circuit.

Press and release the I side of the mains ON/OFF switch to switchthe instrument on again. If the protection circuit keeps tripping

Maintenance

Removing the lamp tube

Fitting the lamp tube

Electrical Protection



73/101



the switch, contact your Termo Service representative.

ELECRICAL PROECION IS INCLUDED O

PROEC HE OPERAOR AND HE INSRUMEN.DO NO AEMP O OVERRIDE HEPROECION RIP. s

Portable Appliance esting (PA) should only be carried out bya competent person. It should only be carried out annually toavoid damaging the equipment. s

Flash testing should only be carried out when absolutely

necessary as damage to sensitive electronic devices may occur. Itmust only be carried out by a competent person. s

Internal fuses are used to protect the electronics within theinstrument. Te fuses should only be changed by a qualifiedservice engineer. s

WARNING

WARNING

WARNING



74/101


Troubleshooting

Troubleshooting

Correct service and maintenance is essential for the long termserviceability of precision engineered products such as the ShandonCryotome. We strongly recommend that a Termo Service Contractis used to ensure future reliability, and consistency of performance.Shandon Cryotome has self test routines built in so that error codesshow in the display if malfunctions occur. You will be advisedwhether you need to contact your Termo Engineer for service.

able 1 lists basic instrument function problems and solutions.

able 2 lists other problems mainly concerned with sectioning andtheir causes that are not shown in the displays.

able 3 lists alarm signals, their causes and the remedial action tobe taken.

If an error indication continues or recurs, remove the samplesfrom the refrigerated chamber and store them in a suitablerefrigerated container and contact your Termo ServiceRepresentative.

Chapter 7



75/101


Troubleshooting

Table 1 - Instrument Function

Symptom Cause Remedy

The Shandon Cryotome does

not respond when the mainspower is switched on

The instrument is still

carrying out initial tests

No mains supply

The circuit breaker has tripped

The mains fuses have blown

Wait approximately 30 seconds

(an hour glass is displayed) for theinitial tests to finish

Connect the power lead switch on

the mains power and instrument

main power switch (see pages......)

Reset the circuit breaker by switch-

ing the instrument off and on again

(see page...)

Replace the mains fuse

Replace the instrument fuses(Note only a technicallycompetent person should

replace fuses)

The Shandon Cryotome is

powered up, but does not

respond correctly

The Shandon Cryotome may have

started incorrectly

Switch off the instrument. Wait for

30 seconds. Switch on the main

power switch

The knife holder or any part

of the knife holder cannot be

clamped or unclamped

Ice and / or debris between the

parts of the knife holder

Full defrost

Remove debris



76/101


Troubleshooting

Table 2 - Problems when sectioning

SymptomCause

Remedy

Specimen cracks as it is frozen Too rapid freezing

Specimen too thick

Reduce cryobar temperature

Cut specimen into thinner block

Specimen falls off cryocassette Specimen too thick

Cryocassette too cold before

mountant added

Specimen unsupported

Cut specimen into thinner block

Reduce time before adding mountant.

9Shoudl be at room temperature)

Give the specimen more support

medium (e.g. Shandon Cryomatrix)

Specimen advances but doesnot cut

Knife loose

Cryocassette loose

Specimen not firmly stuck to the

Cryocassette

Knife angle incorrect

Make sure that the knife is clampedcorrectly

Make sure that the cryocassette is

fitted and clamped correctly

Re-mount the specimen on the Cryo-

cassette using adequate mountant

Increase knife angle

Sections roll up Anti-roll plate too low

Specimen too cold

Raise the height of the anti-roll plate

Raise the specimen temperature

Warm the specimen slightly with a

gloved thumb

Sections thaw when cut Cutting equipment not cold enough Allow more time for the knife and the

anti-roll plate to cool

Cont.../



77/101


78/101


Troubleshooting

Table 2 - Problems when sectioning (cont)

Symptom Cause Remedy

Sections thick and thin Blunt knife or blade

Knife angle too shallow

Specimen not secure

Knife holder is loose

Specimen temperature incorrect

Sharpen knife or use new blade

Widen the knife angle

Re-freeze and secure specimen

Make sure the knife holder is secure on

the base

Raise or lower the temperature

Sections stick to the anti-roll

plate

Dirt or contaminant on the anti-

roll plate

Anti-roll plate edge is damaged

Cutting equipment not cool

enough

Use the brush to clean the anti-roll plate

Change to new edge on anti-roll plate

Allow more time for the knife and the

anti-roll plate to cool

Sections are split vertically The knife is dirty or blunt

The knife/blade, anti-roll plate or

clamp plate are damaged

Remove the knife/clean and sharpen it.

WEAR CUT RESISTANT GLOVES.

Examine the knife/blade, anti-roll plate

and clamp plate and replace if necessary

Sections curl after lifting

from anti-roll plate

Anti-roll plate too warm

Blade blunt

Static electricity in chamber

Lower chamber temperature

Change blade



79/101


Troubleshooting

Table 3 - Error messagesHelp Screens are displayed for each error message

No Cause Remedy

1 Microtome faultA problem has occurred when the software has tried

to initialise the microtome

Make sure that the specimen head is not ob-structed. Press EXIT to reset the microtome.

Remove the transit bracket

Turn the instrument off and retry

If neither of these solutions are successful, call

Thermo Service

2 Transit bracket has been fitted success-fully

Turn off instrument and repack it to move it safely.

If the transit bracket has not been fitted, c lear the

obstruction in front of the head and fully retract the

specimen head.

3 Chamber temperature sensor is faultyThe chamber will be driven at maximum cooling

effect

Call Thermo Service

4 Cryobar Temperature Sensor is Faulty

The cryobar will be driven at maximum cooling

effect

Call Thermo Service

5 Specimen temperature sensor is faulty

The specimen head control has been disabledWait for the specimen temperature to stabalise in

the chamber ambient before sectioning

6 Window error lock Make sure the window is fully closed and retry

7 Fumigation could not start

The window could not be lockedMake sure the window is fully closed and restart

fumigation

8 Fumigation Error

There was a fault during fumigation

THERE MAY BE FORMALDEHYDE

FUMES IN THE CHAMBERs

Call Thermo Service

Cont.../

!



80/101


Troubleshooting

Table 3 - Error messages ( Cont...)

No Cause Remedy

9 System error

A critical fault has occurred which may have resultedin one or more systems being disabled

Call Thermo Service

10 Specimen head ahas overheated

The specimen head control has been disabledWait for 5 minutes for the head to cool down and

then reselect the desired specimen head tempera-

ture

11 Read the text carefully. Error 11 is either:

Cryobar refrigeration system fault

or

Chamber refrigeration system fault

Remove all specimens from the cryobar and chamber

and call Thermo Service

Remove all specimens from the chamber and call

Thermo Service

12 Specimen head temperature control

faultWait for 5 minutes and then reselect the desired

specimen head temperature

13 Voltage Selection Error The instrument needs to be reset for the local mains

voltage. Call Thermo Service.

14 Fumigation heater error

The fumigation heater is on permanently

THE FUMIGATION HEATER IS

VERY HOT - DO NOT TOUCH. s

Call Thermo Service.

!



81/101


Specifications and Accessories

Specification andAccessories

Specification

Physical

Width 830mm (max) (33ins) (including handwheel)

Depth 745mm (29ins)

Height 1050 mm (41 ins) (working height)

1205mm (275 lbs) (instrument height)

Weight 125kg (275 lbs)

Electrical

Supply Voltages 230Va.c. (~) 50Hz 1800VA

115Va.c. (~) 60Hz 1800VA

(Voltage is set at the factory)

Maximum supply voltage fluctuations not to exceed 10% of

nominal voltage

Power demand Notes: characteristics 1. I (max) = Peak Current 2. Peak Current is for 22 secs max with stalled rotor. 3. Voltage drop affects compressor start capability. 4. Supply impedance affects compressor start-up capability

NominalInput

Vac

NominalVoltage@I max

Vac

MaximumVoltage@no load

Vac

Imax - MaximumCurrent whencompressor starts

A

MaximumSupplyImpedance

V/A

230V 50Hz 198 253 12 1.0

115V 60Hz 103 126 38 0.25

Chapter 8



82/101


Specification and Accessories

Fuses should only be replaced by technically competentpersonnelMain plug fuse 13A 250V (where applicable)Main fuses (x 2) F5A 250V (Part number P11171)

Main fuses must only be replaced by service personnelRemote alarm fuse F5A 250V (Part number P11171)(x1)

I Power On

O Power Off

Indoor use only

+5C to +40C

+5C to +35C

NOE - refrigeration performance may be degraded at ambienttemperatures above +25C s

-25C to +55C (+70C for short exposure)

80% max. for temperatures


83/101



230V; 50Hz A78900001

115V; 60Hz A78900002

230V; 50Hz A78900003

115V; 60Hz A78900004

See also page

Rotary rocking microtome mounted outside refrigerated chamber.

25mm

>1mm / second

1 to 20m in increments of 1m

20 to 60m in increments of 5m

57mm

40m on upward stroke

at an ambient temperature of +25C

0 to -35C

-55C (minimum) (adjustable)

0 to -40C (performance restricted to10C from chamber temp)

Part Numbers

Shandon Cryotome FSE

Shandon Crytome FE

Centre of Gravity

221 mm

508mm

317 mm

WINDOWBOOST

Microtome

General

Total specimen advance

Advance Speed

Section thickness range

Cutting Stroke

Retraction

Cryochamber

Cryochamber temperature

Cryobar temperature

Specimen head temperature

(FSE)



84/101


Specification and Accessories

Instrument Accessories Quantity Part Number

Knife / blade holders

Disposable blade holder with anti-roll plate - high profile 1 A78910033

Disposable blade holder with anti-roll plate - low profile 1 A78910032

Solid knife holder with anti-roll plate 1 A78910047

Orientation heads: X/Y orientation head [FE only] 1 A78910016

Cryobar Accessories:

Cryobar cassette identification label pk of 4 A78910054

Cryobar cover 1 A78910016

Cryocassettes:

40mm diameter crosshatched (multicoloured) pk of 6 A78910031

40mm diameter annular pk of 4 A78910055

25mm diameter annular pk of 4 77210150

25mm diameter crosshatched pk of 4 A78910056

9.5mm diameter angular pk of 4 77210153

28 X 40mm crosshatched pk of 4 A78910057

Handle pk of 4 A78910058

Debris Tray 1 A78910039

Heat Extractors: standard 1 A78910014

half weight 1 A78910036

double weight 1 A78910037

Shelves: Tool 1 A78930121

Narrow 1 A78930166

Wide 1 A78930063

Linistat Stainer: 220-240V, 50Hz 1 B1000201

110-120V, 60Hz 1 B1000200

REFER O APPENDIX B FOR A LIS OF APPROVED REAGENS IF A SAINING

SYSEM SUCH AS HE SHANDON LINISA IS USED ON OP OF HE INSRUMEN.

See catalogue for details of solid knives, disposable blades, CryomatrixM

and otherCryostat accessories.

Accessories



85/101



Spares

Instrument Spares Quantity Part Number

Allen key (handwheel transit fixings) 1 P13546

Anti-roll plate - glass (4 usable edges) 1 A78930200

Anti-roll plate - perspex (4 usable edges) 1 TBA

Brush (sections) 1 P12940

Brush (debris) 1 P12257

Fluorescent tube 1 AP14917

Mains leads:

UK 1 P13291

EU 1 P13290

US 1 P13292

Operator Guide (available in different languages) 1 A78910100

Spanner (foot adjustment) 1 A78930178



86/101


87/101


Declaration of ConformityThis Declaration of Conformity is only valid when the instrument is used in accordance with the Operator Guide A78910100

Manufacturers Name:Manufacturers Address:

Product Description:

Product Designation:

Part numbers:

Orientation Heads:

Cryocassettes:

Knife Holders:

Year of Marking (CE):

Thermo Shandon Limited (Trading as Thermo Fisher Scientific)

Chadwick Road, Astmoor, Runcorn,

Cheshire, WA7 1PR

ENGLAND

Cryostat

Shandon CryotomeFEShandon CryotomeFSE

A78900001, A78900002, A78900003, A78900004including accessories supplied as standard and the following accessories:

A78910016, A78910022

77230540, 77230546, A78910031, A78910055, A78910056, A78910057

A78910083

A78910032, A78910033

2005

This product conforms with the essential requirements of the following directives:

In Vitro Diagnostics Directive

Low Voltage Directive

98/79/EC

2006/95/EC

This product complies with the following International Standards:

EMC:

Safety:

Issued by:

EN 61326:1998

EN 61000-3-2:2000

EN 61000-3-3:1995 Inc A1:2001

EN 61010-2-101:2002EN 61010-1:2001

CAN/CSA C22.2 No. 61010.1-04

UL Std No. 61010-1 (2nd

Edition)

K. Waldron

Quality Manager

Thermo Fisher ScientificAnatomical Pathology, Diagnostics Division

Date: 1stJune 2007

Optional accessories considered subject to the In Vitro D iagnostics Directive (IVDD) are specifically identified on this Declaration o f Conformity. Furthersupplies of standard accessories are treated as spares. Convenience aids offered as accessories are not subject to the IVDD.



88/101


Transportation InstructionsIf you ever need to transport the Shandon Cryotome, follow thesepackaging instructions.

SHANDON CRYOOME FE AND FSE WEIGHAPPROXIMAELY 125kg (275lbs).ALWAYS GE HELPO SAFELY MOVE HE INSRUMEN WIHOURISK OF INJURY. s

Follow the instructions in Chapter 6 and follow good laborartorypractice to clean and decontaminate the instrument. Complete andsign the Product Safety Declaration (at the end of this chapter) toconfirm that the instrument is fully decontaminated.

Make sure that all knives, blades and holders are removed from theinstrument. Remove the shelves, heat extractors and tools from thechamber.

Use the Advance Control Panel to position the Specimen Head in

the fully retracted posiiton. Move the specimen head to the top ofits travel.

Fit the transit bracket to the knife holder support plate and slide itto the back of thechamber.

Lower the specimen head into the bracket.

Place the face plate at the rear of the specimen head into the transitbracket clamp and tighten the cap screw on the base of the transitbracket knob. Gradually tighten the other two cap screws.

MAKE SURE HE CAP SCREWS ARE IGH BEFOREMOVING ON O HE NEX SAGE. s

Appendix A

Repacking



89/101


Use the control panel to move the specimen head forward until itjams. Te screen will display an Error 2 warning screen. Switch offthe instrument. Do not acknowledge the error.

Switch off the instrument and remove mains cables from the rear ofthe elctronics box.

Empty the waste bottle and replace it in the instrument.

BE AWARE OF POENIAL BIOHAZARDS ANDFORMALIN CONAINED IN HE WASE BOLE.DISPOSE OF CORRECLY IN ACCORDANCE WIHLOCAL PROCEDURES. s

Remove the handwheel bolt and remove the handwheel.

Lift the top cover and secure the window in the closed position usingsticky tape. urn the instrument lowering knobs counter-clockwiseto lower the rollers to the ground.

Specimen head face plate

Transit bracket cap screws Transit bracket



90/101


SHANDON CRYOOME FE AND FSE WEIGHAPPROXIMAELY 125kg (275lbs).ALWAYS GE HELPO SAFELY MOVE HE INSRUMEN WIHOURISK OF INJURY. s

Use two people for the following steps:

1. Place the packaging pallet in a convenient place, so that thereis enough room for the ramp to lie on the floor in front of the palletand that there is enough room for a person to get to the back of thepallet.

2. Push the instrument backwards up the ramp and make sure

that the instrumnet sits inside the foam packing on the pallet. Tefront of the instrument should face forward.

3. Place the accessories box in front of the instrument underneaththe footrest.

Repacking



91/101


4. Raise the ramp and place the foam packing piece on top of theinstrument, making sure that the top of the ramp fits into the slotin the foam.

5. Wrap the cardboard outer around the instrument so that the joinis at the front next to the raised ramp. Te large flap (with the threeholes on the edge) must be under the small flap (with the threeplastic fixings).

6. Push the plastic fixings so that they match up with the holes inthe large flap, and teist the inner part of the plastic fixing so that thetwo cardboard flaps are joined together.



92/101


7. Put the cardboard lid on top of the packed instrument and finallybind the complete package so that the bingings run over the lid andthrough the pallet.



93/101


A78910100 Issue2110 Shandon Cryotome Operator Guide

PRODUCT SAFETY DECLARATION

Part 1 DECONTAMINATION CERTIFICATE

Any instrument, or part of any instrument, must be clean before being returned or before any on-

site service, and where necessary accompanied by a completed Decontamination Certificate. If the

instrument, or any part of it, is found to be in an unclean condition upon receipt or before a service,

or Thermo Fisher Scientific consider it to be a hazard, the instrument or part will be returnedunrepaired at the expense of the customer or service will be refused.

In the case of returns, it is important that the certificate is forwarded by post or fax, and a copy

attached to the exterior of the container. Containers will not be opened until the company is in

possession of the required certificate. This form MUST be completed by the customer and NOT a

Thermo or distributor employee.

If the instrument or any part of it is to be returned to Thermo and has been exposed to, or been in

contact with potential pathogenic or radioactive material, it is essential that it is decontaminated.

For decontamination, reference should be made, where appropriate, to the European Directive

2000/54/EC. To avoid any misunderstanding, we request that all in

Cryotome FE & FSE Operator Guide A78910100 Issue 4

Documents

Transcript of Cryotome FE & FSE Operator Guide A78910100 Issue 4