CRISPRSetting New Frontiers

Cho Han, ChiangNational Taiwan University2016/5/15

Outline

1. Introduction to CRISPR and gene editing

2. Development of CRISPR: medicine, agriculture, environment

3. Pros and Cons of CRISPR

4. The future

What is CRISPR?

Gene editing

Guide RNA

Cas9 protein

Components of CRISPR

CRISPR has Many applications

CRISPR in medicine

HIV/AIDS

Other medical applications

Muscular Dystrophy

Cancer

And Many more..

CRISPR in agriculture and environment

 Reduce hunger and poverty

From: United Nations Environment Programme

Improve nutrition, health, and rural livelihoods

Facilitate social and environmental sustainability development

More nutritious More resistant to climate and pests

Arabidopsis thalianaTobacco plant

CRISPR in agriculture and environment

Prospects are good, but…be cautious

Perspectives

Many applications, limitless potential

“With great power comes great responsibility”

References:1. Development and Applications of CRISPR-Cas9 for Genome Engineeringhttp://www.cell.com/cell/abstract/S0092-8674(14)00604-72. The CRISPR/Cas9 system for targeted genome editinghttp://www.clontech.com/US/Products/Genome_Editing/CRISPR_Cas9/Resources/About_CRISPR_Cas93. CRISPR IS COMING TO AGRICULTURE — WITH BIG IMPLICATIONS FOR FOOD, FARMERS, CONSUMERS AND NATUREhttp://ensia.com/voices/crispr-is-coming-to-agriculture-with-big-implications-for-food-farmers-consumers-and-nature/4. Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoidshttp://www.nature.com/nm/journal/v21/n3/full/nm.3802.html5. Agriculture: A new breed of editshttp://www.nature.com/nature/journal/v528/n7580_supp/full/528S15a.html6. CRISPR the disrupterhttp://www.nature.com/news/crispr-the-disruptor-1.17673

Thank you for listening. Questions?