Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945
description
Transcript of Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945
![Page 1: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/1.jpg)
Course: Biol 401Instructor: Dr. Alison Crowe
426A Hitchcock [email protected]
Office Hours: Wed 4:30-5:30 PMHCK 426Aor by arrangement
Teaching Assistant: Qing Feng [email protected] Office Hours: TBA
NOTE: CHANGE OF ROOM FOR AB TO LOW 216
![Page 2: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/2.jpg)
Required Text: Lodish et al., 2013, 7th edition, Molecular Cell Biology
Required Course Manual: Professional Copy’n Print4200 Univ. Way NE
Course website: http://mesh.biology.washington.edu/biol401-spr13/index.html
You will need the following info to accessthe readings folder on the course website:
Login: biol401 Password: cell
![Page 3: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/3.jpg)
Schedule in course manual: Topics, Assignments, Due datesImportant dates:
Midterm: April 30 10:30-11:20 AM MGH 231Final: June 10:30AM-12:20 PM MGH 231
Discussion sections are mandatory
Readings: To be completed each week BEFORE lectureand discussion sections
This week: How we study cells & protein trafficking
Overview of cell biology approaches: Chp 1.2 p. 10-15 (Fig. 1-13, 1-14)Gene and protein tagging: Chp 5 pp. 203-205 (Fig. 5-34)Organelles of the eukaryotic cell: Chp. 9 pp. 424-427 (Fig. 9-32) Overview protein trafficking: Chp. 13 p. 577-579 (Fig. 13-1)
![Page 4: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/4.jpg)
Individual PointsDiagnostic Test 3W.A. #1 - nuclear transport I 15* W.A. #2 – nuclear transport II 10W.A. #3 – critical analysis 10W.A. #4 – figure analysis 5MCQ answers 5Practice Midterm 5 Midterm Exam 60Final Exam 90 pts
Subtotal 203 pts (70%)
POINTS AVAILABLE IN CLASS
* Due in Lecture Next Week
![Page 5: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/5.jpg)
Group PointsReading Quiz #1 (group) 5Reading Quiz #2 (group) 5Nuclear Transport Experiment 5Group presentation of figures 10 W.A. # 5 – Critical Analysis 20 pts
Subtotal 45 pts (16%)
Group Participation ScoreGroup Assessment #1 20Group Assessment #2 20 pts
Subtotal 40 pts (14%)
Total 288 pts*Access your scores on course web page: “scores” link on left-hand menu
![Page 6: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/6.jpg)
Learning Goals for Course:• Evaluate the relative merit of using a particular molecular technique to address a specific research question • Interpret cellular and molecular data (e.g. gels, graphs)• Predict outcomes of future experiments based on existing data• Develop new hypotheses and design experiments to test those hypotheses• Draw a model for a molecular process based on existing data• Recognize assumptions inherent in a given molecular model• Evaluate the merits of a scientific study•Communicate scientific ideas and/or interpretations articulately, both in writing and orally.
![Page 7: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/7.jpg)
Expertise
Imag
inat
ion
John Bransford
Routineexperts
Rudderlesslearners
Adaptiveexpertssynthesis
evaluation
application
analysis
knowledge comprehension
How People Learn
![Page 8: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/8.jpg)
Course Outline•Protein Trafficking
•Nuclear transport•Nuclear environment
•Nuclear pore structure•Spatial and dynamic organization of nucleus
•Eukaryotic transcription regulation• Epigenetic regulation• Cell memory• Cell specialization
• Embryonic Stem Cells• Epigenetic modifications during differentiation• Maintenance of pluripotency
![Page 9: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/9.jpg)
Review organelles in readings: know primary function of cell organelles
Fig. 1-11, 1-12. Lodish et al. 2013
What are the advantages and challenges of eukaryoticand prokaryotic cells?
![Page 10: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/10.jpg)
To understand how genes are regulated, need to understand the nuclear environment: structure, spatial organization
Know these Terms:Nuclear envelope (NE)Nuclear pore complex (NPC)Nuclear laminaNucleolusChromatin (heterochromatin and euchromatin)
![Page 11: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/11.jpg)
Protein Sorting Animation
Overview of Protein Trafficking
Signal sequences on proteins target them to locations within the cell
![Page 12: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/12.jpg)
Protein Trafficking Workshop
You have the following tools:A cell into which you can introduce (tranfect) DNAA DNA plasmid into which you can clone any gene you likeA fluorescent-labeled antibody to any protein you want to detectA piece of double-stranded DNA encoding a potential mitochondrial signal sequence
![Page 13: Course: Biol 401 Instructor: Dr. Alison Crowe 426A Hitchcock Hall 616.6945](https://reader036.fdocuments.in/reader036/viewer/2022070416/568150bb550346895dbed825/html5/thumbnails/13.jpg)
Signal Sequences Have Been Identified Which Target Proteins to Locations Within the Cell