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Family Lactobacillaceae

Shape: Rod

+ fa - - 4 - A AS AS A - - A - - - - - - - - of/f

+ fa - - 1 - A - A - - A - - - - - - - of/f

+ fa - - 4? - A - A A A - - - - - - - of/f

Family Micrococcaceae

Shape: Cocci >12 >28 >20

+ a/m - V 1 - AS - - - - - -/w - - - - g - V S +i + - o +++ + ++ a - - 1-2 - AS - - AS - - - - - - - g - - S +i +S - o +++ + ++ a V + R - AS - - - - A ws - - - G g - - + +i + - o +++ + ++ fa - + 4 - A A A A - AS A - +w + - b - + +i - - of/f - v -w

+ fa - - 4R - A V A - - AS V - V - - - - + +i - - v - -+ aa - - 6 - A A A - - - A - - - g - - - - - - of/f - - -+ fa - - 6 - A A A AS - - A - +w - - g - - + +i - - of/f v +/w +w

+ fa - - 1 - A - AS A - A - - V - - αw/- - - + +i +S - of/f w + +

Family Streptococcacaea

Shape: Cocci >12 >23 >24

+ fa - V 6 - A A A - - A A - - b - - - - - -? + ++ fa - - 4R - A A A A V A - - a/g - - - - - - -? + + ++ fa s/V - 4R - A A - A - A A - - g - - - - - -? w v v+ fa - - 4 - A AS AS A - - A - - g - - - - - - of/f

1. Lactobacillus pluturaum

2. L. delbrueckii

3. L. casei

1. Micrococcus luteus

2. Kocuria. rosea

3. K. varians

4. Staphylococcus aureus

5. Staphylococcus epidermidis αw6. Anaerococcus tetradius

7. Staphylococcus saprophiticus

8. Staphylococcus capitis

1. Streptococcus pyogenes

2. Streptococcus mutans

3. Enterococcus durans

5. Lactococcus lactis

M4

cannot confirm fr Bergey's

M5


O5

From bergey's discription of the spps

M6


M10

Bergeys syas this is A

N10

Bergey's says it is - the weak color may be from the pigment in the cells

V10

our strain is negative but most are positive

A11

old name Micococcus roseus

L11

Bergey's says it is V

AE11

from Bergey's

A12

old name Micococcus varians

D12

in a wet mount, there may be slight motility

E12


I12

Bergey's table says it is A, but his text says it is negative, I think our strain is negative

L12


M12

Bergeys

N12

Bergey's says it is -

F14

some strains are only R if they are lactose negative

A15

old name Peptostreptococcus tetradius

W15

this is because it is an anaerobe

AB15

this cannot be 'of' as it is an anaerobe

A16

variant spelling Staphylococcus saprophyticus

I16

Our strain is A, but Bergey's says it is V

K16

Bergey's says it is V, our strain is A but slow

M16

Bergeys syas this is A, my result was negative

N16

Bergeys says it is -

Z17

requires 2 weeks at room temp.

L22

fr/ Bergey's

A23

shape may have some short rods

L23

Bergey's says it is +, but ours is V as it grows poorly on starch plates

W23

Bergey's says 6.5% is V, we use 7.5% and its -

W24

I have had variable results, perhaps fr/ errors in making MSA. Troy's strain is negative

A25

are cocci but the cocci are stretched in the direction of the chain in streptococci

M25


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Family Enterobacteriaceae

Shape: Rod >20

1. Escherichia coli - fa + - 4RG - AG AG V AG - A A + - + V - - - - +s + - of/f v2. E. adecarboxylata - fa V - 5R - AG AG V A A A + - + - - - - +s + of/f v3. Enterobacter aerogenes - fa + - 5R - AG AG AG AG - A A - + - - B V - +S +s + of/f v4. E. cloacae - fa + - 4RG - AG AG AG AG V A A - + - - B - - +S +s + +S of/f v5. Klebsiella pneumoniae pneumoniae - fa - - 6 - AG AG AG AG A A - + - - B + - +s + of/f v6. Erwinia amylovora - fa + + 4R - A - A - - A - - + - B - +s - - of/f +7. Serratia marcescens - fa + + CR - A - A A - A - - + - - B - - - +iw/s +s of/f -w8. S. fonticola - fa + - 4 - AG AG - AG A - - - + - B - - +iw +s v11. Morganella morganii morganii - fa + - 2 - AG - - - - + - + - - - - +i + of/f +12. Salmonella choleraesius - fa + - 2 - AG - - AG - - - - + + - V - +s + of/f

15. Citrobacter freundii - fa + - 6R - AG AG AG AG - A A - - + V B - - - +s + of/f v16. Shigella dysenteriae - fa - - 2 - A - - A - - V - + - - - +s +17. Sh. flexneri - fa - - 2 - A - - A - A + - + - - - - +s +18. Proteus rettgeri - fa +S + R - AGS - AGS - - A A + - + + - - - - +i - - of/f +w

Family Mycobacteriaceae

Shape: Rods (positive f/ Acid Fast)

1. Mycobacterium smegmatis + a - - 2 f - - - - - - - - - ### +s + -2. M. bovis + a/m - - f - - - - - - - - - - - -(M smegmatis is a fast grower, dt in hours, grows at 45 C. M. bovis is a slow grower, dt ~1day, no colonies till day 7+, no growth at 45 C)

A33

new name: Leclercia adecarboxylata

D33

most 79% are motile

A34

Species name is in debate. Some call it as here, others as Klebsiella mobilis or Klebsiella aerogenes. It is more closely related to Klebsiella than Enterobacter.

E34

Bergey's agrees, but it is really slow & does not show in 2 wks.

H34

bergeys donfirms

O34

bergeys confirms

R34

bergeys confirms

S34

bergeys confirms

A35

can fix N2, can be in your gut, may cause opportunistic infections.

E35

It needs more than 2 wk to show S.

D36

bergys confirms

H36

bergeys donfirms

O36

bergys confirms

P36

from Bergey's

R36

bergeys confirms

S36

bergeys confirms

V36

bergeys & web confirmed

A37

common name is fire blight, it cause disease on fruit trees, optimum temp is 27-30 C

C37

confirmed w/ web

D37

Bergey's confirmed

E37

Bergey's confirmed

H37

Bergey's confirms

I37

bergeys confirms

J37

Bergey's confirms

K37

our strain is negative but most are A

L37

bergeys confirms

M37

Bergey's confirms

O37

bergeys confirms

P37

Bergey's says it is +, web says it -

R37

Bergey's confirms

S37

Bergey's just says it is +

V37

bergeys & web confirmed

Z37

Bergeys confirms it need growth factors

A38

our strain is clearly different fr/ Bergey's S. marcescens biogroup1

D38

Bergeys confirms

I38

bergeys confirms

O38

Troy's strain maybe Indole positive. The new strain has much less red pigment.

R38

bergeys confirms

S38

Bergey's confirms

Z38

slow and color may be light

Q39

bergey's confirms

R39

bergeys confirms

A40

subspecies morganii old name: Proteus morganii

E40

bergys confirms

O40

bergeys confirms

P40

bergeys confirms

R40

most are negative though a few are +, ours is -

AE40

bergey's

A41

new name: Salmonella enterica subspecies choleraesius (spps has many subspecies)

I41

bergeys confirms

O41

bergeys confirms

R41

Bergey's confirmed

S41

most are - by Bergey's

I42

most, our's is A*

O42

a few strains are positive, ours like most are negative

R42

most are positive

V42

after 45 seconds it appears to be positive so watch the time closely.

E43

bergeys confirms

H43

according to Bergey's it does not ferment glucose but does mannitol. I think that is impossible

I43

bergeys confirms

J43

bergeys confirms

P43

bergeys confirms

Q43

bergey's confirms

S43

bergeys confirms

E44

bergeys confirms

I44

bergeys confirms

J44

bergeys confirms

P44

bergeys confirms

Q44

bergey's confirms

S44

bergeys confirms

A45

alternate spelling Proteus retgeri, may have a new name Providencia rettgeri

S45

after >1 week, G

B50

Gram positive but difficult to stain

S50

after >1 week, G

B51


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Family Achromobacteriaceae >29Shape: Rod

1. Alcaligenes faecalis - a + - 2 - - - - - - - - - B a V + - +i - - -5. A. latus - a + + 2 + A - - V + +i - - of/f

2. Achromobacter denitrificans - a + - 2 - - - - - - - - - - - B + +i - - -3. Achromobacter xylosoxidans - a + - 2 A - - - + V - B + +i - - oS4. Ralstonia eutrophus - a + - 3R A A - - - A - - B + +i - - oS -6. Variovorax paradoxus - a V 3 A - - A - A - - B + +i + - oS +

Family Bacillaceae

Shape: Rod >12>281. Bacillus cereus + a/fa + + CR + A - A - + AS A - + w - G b v + +S +iw/s + +S v - -2. B. subtilis + a/fa V + CR + A - A A + A A - + -/w B a/g + + +i/s + - v - +3. Paenibacillus polymyxa + fa + - 5 + AG AG AG AG + A A - + -/w - g - - +i/s - - v v4. B. megaterium + a/fa V + 6 + A A A A V V ws - - - - B g v + + +s + + v + v

Family Corynebacteriaceae

Shape: Rod but pleomorphic

1. Corynebacterium xerosis + V - 1 - A - A - - A - - - g - +i - - o2. C. pseudodiphtheriticum + fa - 1 - - - - - - - - - - B g - + - - -

AD56

this is not an official cutoff value

A58

opportunistc urinary infections

H58

can be recorded as K, alkaline

I58


J58


K58


M58


W58

might see insignificant growth with time, does grow in slightly less salt according to Bergey's

A59

this species may have a new name and probably is not in Alcalignenes short rods approaching coccobacilli

H59

cannot confirm A in Bergeys, switching to blank

A60

old name Alcaligenes denitrificans

A61

old name Alcaligenes xylosoxidans

A62

also eutropha old name Alcaligenes eutrophus

A63

old name Alcaligenes paradoxus colonies are pigmented

E63

Bergey's has this as V in one table and as negative in a talbe on this spps

AC67

This inhibition zone is not an official value, as Bacitacin is not used w/ Bacillus. I made it up because of stark differences w/i this family

AD67

This inhibition zone is not an official value, as Penicillin is not used w/ Bacillus. I made it up because of stark differences w/i this family

A68

causes food poisoning, enterotoxins & other toxins produced

G68

position of the endospore can also be used to identify the species

J68

ours is A, Bergey's says it is V

M68

bergey's agrees

V68

bergey's says it is negative

AA68

need two weeks at room temp to be certain

C69

bergey's says this is aerobic

G69


I69

our strain is -, but Bergey's says it is V (11-89% +)

M69

bergey's

P69

bergeys confirms

V69


A70

old name Bacillus polymyxa fixes N2, lives w roots, makes polymyxin antibiotics

E70

our strain differs from most of bergeys

G70

position of the endospore can be used to identify the species, it swells the mother cell

P70

bergeys confirms

Z70

biotin is required

AA70

biotin is required

C71


D71

my strain is - in Sims, bergeys says its motile

G71


L71

bergeys says it is + our strain is -

M71

bergey's MY RESULTS WERE NEGATIVE

P71

is for most strains

V71


C76

Bergey's says 90+% are fa, but my testing show our strain is an a (an aerobe)

Z76

Bergey's says growth factors required

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Family Neisseriaceae

Shape: Varies >39

1. Neisseria sicca (coccus) - a - 0 - A - A - A - - - V + +i - o2. N. subflava (coccus) - a - - A - V V - - g + +i - o3. N. lactamicus (coccus) - a - - A A - - A - g + +i - o

- a - - 1 - - - - - - - - -/V g + +i - - - +(29)5. Acinetobacter calcoaceticus - a - - 1 - A - - - - B g - +i + - o >20

6. N. toyus (coccobacillus) - fa + - 4RG AG AG AG AG - A A - - - - B g - - +s + +S of/f 0 0 -w

Family Pseudomonadaceae

Shape: Rod >20

1. Pseudomonas aeruginosa - a + + 3R - - - - - - - - - - B b - + - +i + - -2. P. fluorescens biovar I - a +S S 1/2R - - - - - - - - - - B - S +i + - -

General Key: Litmus Milk Key: Key to Fermentation Broth:+ = positive result 0=No Change, little growth if any A=Acid- = negative result 1=No change, growth A*=Acid and gasS = positive but is slow 2=Alkaline AS= >24hr for slow acidi = positive and immediate 3=Alkaline with peptonization - = Negative or no change or alkalineW = weak results 4=Acid, clot formationV = variable results 5=Acid and gas Key to Other Tests:blank = test is not used, treat as V 6=Acid, no curd B=blue color or blue and growth (Citrate Test)f = false positve C=Curd (not acid) G=growth but no color change (Citrate Test)

R=Signifies reduction a=aerobeaa=anaerobefa=facultative anaerobem=microaerophilea,b,g = alpha, beta, gamma hemolysisof/f=oxidation and/or fermentation

4. Moraxella catarrhalis (cocci)

αw

A84

All the three Neisseriaceae species here frequently have diplococci though they have other growth patterns.

X86

catalase immediate is unconfirmed, Bergeys does not say

A88

also called Neisseria lactamica

N88

UNCONFIRMED

A89

change in shape was from Bergey's description

AD89

sensitive to penicillin, > 29 mm. Chart does not this family, so I am not sure where the sensitivity starts.

A90

bacillus but old cells are cocci

V90

bergey's

AE90

antibiotic sensitivity differs from other members of this group, has to be >20 mm to be sensitive

A91

when grown in liquid, small cocobacillus, in Simmons Citrate is short rods

E91

It is slow but needs more than 2 wk to show S.

K95

bergeys confirms

D96

bergeys confirms

E96

bergeys confirms

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Family Lactobacillaceae

Shape: Rod

+ 4 - fa - -

+ 1 fa -

+ 4? fa -

Family Micrococcaceae

Shape: Cocci

+ V 1 +s a/m -

+ - 1-2 - a + -

+ + R +s a +

+ + 4 fa + w

+ 4R +s fa +w - -

+ 6 aa - -

+ 6 - fa - - +

+ +? 1 - fa V -

Family Streptococcacaea

Shape: Cocci

+ 6 fa - - -

+ 4R - fa -

+ 4R - fa - + -

+ fa V

+ 4 - fa - -

1. Lactobacillus pluturaum

2. L. delbrueckii

3. L. casei

1. Micrococcus luteus

2. Kocuria rosea

3. K. varians

4. Staphylococcus aureus

5. Staphylococcus epidermidis

6. Anaerococcus tetradius

7. Staphylococcus saprophiticus

8. Staphylococcus capitis

1. Streptococcus pyogenes

2. Streptococcus mutans

3. Enterococcus durans

4. Streptococcus salivarius

1. Lactococcus lactis

A4

is two species but they have the same test results

C10

tween 80 instead of corn oil

F10

turned white after 2 wks but turns blue when oxygen is readded such as by jostling the tube.

A11

old name Micococcus roseus

C11


A12

old name Micococcus varians

C12


H12

Bergey's table says it is negative, but his text says it is usually positive

E14

some strains are only R if they are lactose negative

J14

Bergey's says this is +, I do not know + to the fast or slow reaction. I did not see this when I did it.

A15

old name Peptostreptococcus tetradius

A16

variant spelling Staphylococcus saprophyticus

F16

after 2 wks may look a lighter color of blue

A26

is two species but they have the same test results

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Family Enterobacteriaceae

Shape: Rod

1. Escherichia coli - - + 4R* - fa + A -

2. E. adecarboxylata - - + 5R fa + - V3. Enterobacter aerogenes - - + 5R fa + A -4. E. cloacae - - V 4R* + fa + A V5. Klebsiella pneumoniae pneumoniae - - + 6 fa + A +6. Erwinia amylovora - - 4R fa - V -7. Serratia marcescens - + V CR + fa + A -8. S. fonticola - - V 4 fa + A -11. Morganella morganii morganii - - + 2 fa + - +12. Salmonella choleraesius - - + 2 fa + A -14. S. typhi - - + 1 fa + -15. Citrobacter freundii - - + 6R - fa + A V16. Shigella dysenteriae - - V 2 fa + V -17. Sh. flexneri - - V 2 fa + V -18. Proteus rettgeri - + + R - fa + - +

Family Mycobacteriaceae

Shape: Rods (positive f/ Acid Fast)

1. Mycobacterium smegmatis + 2 a +

2. M. bovis + - a/m - +(M smegmatis is a fast grower, colonies b7 4-7 days at 35 C and grows at 45 C. M. bovis is a slow grower, no colonies by day 7, no growth at 45 C)

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A32

new name: Leclercia adecarboxylata

D32

most strains

J32

from Bergey's 48% are +, 52% are -

A33

Species name is in debate. Some call it as here, others as Klebsiella mobilis or Klebsiella aerogenes. It is more closely related to Klebsiella than Enterobacter.

J33

bergeys confirms

A34

can fix N2, can be in your gut, may cause opportunistic infections.

D34

70 % of strains do not

J35

bergeys confirms

A36

common name is fire blight, it cause disease on fruit trees, optimum temp is 27-30 C

G36

confirmed w/ web

H36

bergeys confirms

J36

bergeys confirms

A37

our strain is clearly different fr/ Bergey's S. marcescens biogroup1

D37

75% are +

J38

Bergey's says that only 13% are positive

A39

subspecies morganii old name: Proteus morganii

J39

bergeys confirms

A40

new name: Salmonella enterica subspecies choleraesius (spps has many subspecies)

D43

75% +

D44

30% +

A45

alternate spelling Proteus retgeri, may have a new name Providencia rettgeri

C45

most but not all strains

D45

most but not all strains

B49


B50


C50

tween 80

Family Achromobacteriaceae

Shape: Rod

1. Alcaligenes faecalis - - - 2 - a - -

5. A. latus - + V 2 a +2. Achromobacter denitrificans - - V 2 a + -3. Achromobacter xylosoxidans - - - 2 a + -4. Ralstonia eutrophus - + - 3R a + -6. Variovorax paradoxus - + A 3 a V +?

Family Bacillaceae

Shape: Rod

1. Bacillus cereus + - CR + a/fa V V

2. B. subtilis + V CR + a/fa + -3. Paenibacillus polymyxa + + 5 fa + -4. B. megaterium + + 6 + a/fa V -

Family Corynebacteriaceae

Shape: Rod but pleomorphic

1. Corynebacterium xerosis + - 1 V V -

2. C. pseudodiphtheriticum + - 1 fa + +

Gra

m S

tain

Lip

ase

Tar

trat

e

Litm

us M

ilk R

eact

ion

Met

hylb

lue

Mil

k

Oxy

gen

Req

uire

men

ts

Nit

rate

Red

ucti

on

Sorb

itol

Ure

a H

ydro

lysi

s

Family Neisseriaceae

Shape: Varies

1. Neisseria sicca (coccus) - + 0 a -

2. N. subflava (coccus) - V a -3. N. lactamicus (coccus) - - a -

A57

opportunistc urinary infections

C57

tween 80 and not corn oil

A58

this species may have a new name and probably is not in Alcalignenes

C58


A59

old name Alcaligenes denitrificans

C59


D59

most strains +

A60

old name Alcaligenes xylosoxidans

C60


A61

also eutropha old name Alcaligenes eutrophus

C61


A62

old name Alcaligenes paradoxus colonies are pigmented

C62


J62

not sure if data is fr/ the strong or the weak Urea test

A67

causes food poisoning, enterotoxins & other toxins produced

H67

bergey's says that 11-89% are +

G68


A69

old name Bacillus polymyxa fixes N2, lives w roots, makes polymyxin antibiotics

G70


H70

bergey's says that 11-89% are +

G75

Bergey's says 90+% are fa, but my testing show our strain is an a (an aerobe)

C82

tributyrin used as the lipase instead of corn oil

A83

All the three Neisseriaceae species here frequently have diplococci though they have other growth patterns.

H85

change made fr/ Bergey's

C86

about 50% +

A87

also called Neisseria lactamica

- - 1 a + - -5. Acinetobacter calcoaceticus - - 1 a -6. N. toyus (coccus) - 4RG + fa + -

Family Pseudomonadaceae

Shape: Rod

1. Pseudomonas aeruginosa - + 3R a + - -

2. P. fluorescens biovar I - + 1/2R a + -

General Key:+ = positive result

- = negative result

S = positive but is slowi = positive and immediateW = weak results

V = variable results

blank = test is not used, treat as V

f = false positve

4. Moraxella catarrhalis (cocci)

A88

change in shape was from Bergey's description

C88

of tween 80 and not corn oil

A89

bacillus but old cells are cocci

H89

change made fr/ Bergey's

J94

Atlas p155

H95

bergey's confirms

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