Control

Ad LacZ

WT islets treated O/N in low and high glucose. Real-time RT-PCR for Xbp-1 splicing

SAMPLE Xbp-1 T Xpb-1 S TubulinLO-1 21.82 22.05 21.15

21.74 22.04 21.18LO-2 22.78 23.16 21.34

22.59 23.66 21.41HI-1 21.5 21.66 20.28

21.57 21.69 20.71HI-2 21.23 20.4 20.24

21.27 20.61 20.35

S-TLO-1 0.23

0.3 Baseline = 17cyclesLO-2 0.38

1.07HI-1 0.16

0.12HI-2 -0.83

-0.66

Ave S-T Ave S-TLO-1 0.265 LO 0.495LO-2 0.725 HI -0.3025HI-1 0.14HI-2 -0.745