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Quality Products. Lifetime Care.

CONTIFUGE® STRATOSCONTINUOUS FLOW CENTRIFUGE

CONTIFUGE® STRATOSCONTINUOUS FLOW CENTRIFUGE

The mobile separation expert

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Simply betterAssembling the rotor is veryeasy. The empty rotor can beused after just a few simplesteps. The rotor, turning at lowspeed, is then filled with apump.

Time is moneyCompared with discontinuouscentrifuges, continuous flowoperation with the Contifugestratos enables extremelyshort processing times. Timesavings of up to 80% arepossible.

What counts is mobilityThe Contifuge stratos is theonly mobile table top centri-fuge of its kind in the world.With the mobile centrifugestand, it can be effortlesslytransported from one locationto another at any time.

LIGHTNING FAST AND MOBILELIGHTNING FAST AND MOBILE

Kendro is one of the leading international manu-

facturers of high quality laboratory centrifuges. With the

Contifuge® stratos, Kendro has developed a unique and

innovative continuous flow system that enables rapid,

reliable separations of large volumes of suspension

with a low solid matter content. It is therefore ideal for

harvesting bio-products and the examination of water

samples.

The Contifuge stratos is offered as a practical table top

unit which, with the help of a trolley (available as an

accessory), can be used almost anywhere.

Why choose the Contifuge stratos?

Optimal controlThe Contifuge stratos controlsthe peristaltic pump. It intelli-gently ensures that the pumpswitches off during accelerationand braking phases and whenthe minimum speed falls below4000 rpm.

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Utmost reliabilityThe Contifuge stratos operatesextremely reliably when com-plex samples are processed.Even the introduction of air/lather does not affect thesystem’s operation.

Ease of cleaningOnce the centrifuge run iscompleted, the sediment canbe removed with ease.Cleaning and drying of therotor is equally straight-forward.

Lid closedTwo locks ensure that the lidcloses automatically whenpushed gently.

Fully autoclavableExpensive rotors made oftitanium or a special aluminiumalloy are fully autoclavable.This makes the Contifugestratos particularly suited tobio-science and medicalapplications.

Active run stabilityThe Contifuge stratos has aunique spindle drive, activedamping and a motor that isfully decoupled from the rotor.

This means that instrumentmovement and vibration duringoperation are not transferred tothe sample.

Rotor diversityThe Contifuge stratos can bequickly and easily convertedfor discontinuous operation.Eleven different rotors and avariety of adaptors are availa-ble for highspeed operation.The system therefore has themost flexible range of acces-sories available in the entiretable top centrifuge market.

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Die Einsatzmöglichkeiten der Contifuge stratos

THE NUMBER 1 IN LIFE SCIENCE

The Contifuge stratos wasdeveloped for the separationof low density particles fromaqueous suspensions with alow solid content – a sophisti-cated task. This mobile, timeand cost saving system isideally suited to a number ofdifferent applications.

Areas of application

THE NUMBER 1 IN LIFE SCIENCE

Sewage examinations■ Separation of sediments and

suspended matter

Limnology■ Extraction of sediments and

suspended matter from in-land water

Marine research■ Enrichment of plankton

Drinking water purification■ Extraction of suspended

matter as an alternative tofiltration

Brewery process engineering■ Separation of yeasts

Pharmaceutical industry■ Pelleting of proteins/protein

complexes, e.g. growthfactors

Dairy industry■ Preparation of starter

cultures for the dairy industry

Chemical industry■ Concentration of metal and

plastic particles, sedimen-tation of metal hydroxides

Biochemistry■ Harvest of plant and animal

cell cultures, precipitation ofprotein complexes

Cytology■ Enrichment of cells: bacteria,

algae, yeasts, mammaliancells

Medicine■ Harvest of human and ani-

mal cell cultures

Immunology■ Separation of immunisation

globulins/vaccine production,preparation of macrophages

Microbiology■ Harvest of bacteria cultures

and virus lysates

Gene technology■ Cultivation of recombination

bacteria, yeasts and mam-malian cells

Biotechnology■ Harvest of fermenter cultures

in large quantities

This centrifuge has proven itsworth during harvests offermenter cultures and exami-nations of many different watersamples.

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A CONVINCING COMPARISON

Faster as a result of continuity

A CONVINCING COMPARISON

Ideal for large volumesThe Contifuge stratos – a high-speed table top centrifuge withcontinuous flow operation –fulfils a particularly importanttask in the Biofuge family:it can process large volumes(e.g. 50 litres) from fermentercultures, for instance, quickerand more effectively than con-ventional centrifuges.

No transfer, no division into portionsIt therefore represents a con-venient alternative to conven-tional batch processing. Labo-rious transfers and division offermenter contents are nolonger necessary.

Enormous time savingsDiscontinuous centrifugation isvery labour intensive and timeconsuming where large volumes

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7 runs at 20 mins.7 x loading and unloading at 5 mins. = 2 hrs 55 mins. 1 run: 40 mins.

500 ml/min500 ml bottles

40 bottles/40 pellets 1 rotor/1 pellet

Discontinuous centrifugation

Continuous centrifugation

are involved. Depending on therotors’ capacity, repeated runsare necessary. The cell harvestis made up of a large number ofrelatively small pellets.

Time savings when processinga bacterial culture: ■ Using a floor standing centri-

fuge with a 6 x 500 ml rotor(batch processing), 20 l ofculture was harvested in 2hours and 55 minutes.

■ With a Contifuge stratos,only 40 minutes were neededto process the same amount.

Very practical – everything’s in the rotor■ Whereas 40 different pellets

are generated with batch pro-cessing, the entire bacterialculture is contained inside therotor when using theContifuge stratos.

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COMPACT SYSTEM FOR LARGE VOLUMESCOMPACT SYSTEM FOR LARGE VOLUMES

And it all works very easilyThe fixed rotor head with inletand outlet rises above thecentrifuge lid.

Using a peristaltic pump, thesuspension is fed into the rotorbody via a rotating joint. Thesample flows through thechamber, and sediment par-ticles gather against the rotorwalls, while clarified medium isremoved.

When the suspension iscompletely processed or therotor’s intake capacity isreached, the centrifuge isstopped. The rotor is thenopened, and the pelletharvested.

Intelligent centrifugation –Contifuge stratosThe Contifuge stratos sensiblycombines the benefits of amodern highspeed table topcentrifuge with the continuousflow principle.

This model is characterised byparticularly intelligent techno-logy. With its unique spindledrive, the electronic imbalancerecognition system and theEasycontrol 2 operating panel,the Contifuge stratos is one ofthe most powerful systemsaround.

High RCF values allow thesedimentation of large volumesin biotechnology and for wateranalysis.

sediment

inflow

rotating body

outlet

fixed rotor head

sealing liquid (glycerine)

Separation of particles by means of centrifugation in a Heraeus continuous flow rotor

organism E. coli

volume 20 l

optical density (578 nm) 1.2

rotor 75003049

speed 15,000 rpm

flow rate 320 – 650 ml/min

Test parameters

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8000

400 600

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flow rate [ml/min]

100

95

90

85

80

200

Centrifugation separation capacity

EXAMPLE OF POSSIBLE APPLICATION – CELL HARVESTINGEXAMPLE OF POSSIBLE APPLICATION – CELL HARVESTING

Macrophages from cattle lungs 7 – 20 760 4,100 n.d.Plankton 1 – 5 200 6,000 n.d.

Suspended matter fromRiver Spree water (heavy metal) 0.2 – 1 360 17,000 n.d.

Example applicationThe harvest of an Escherichiacoli culture with a straight,stick-like bacterium (1.1 – 1.5 x 2.6 – 6.0 µm) is describedbelow.

20 l of an E. coli culture in a LB medium were harvested at 15,000 rpm with a varying flowrate. The optical density wasdetermined from the residueand the percentage degree ofclarification was calculatedfrom it, i.e. the centrifugationseparation capacity was ascer-tained.

ResultThe degree of clarification,depending on the flow rate, is illustrated in the graph. An in-crease in the flow rate inexcess of 500 ml/min wasconnected to a considerabledecrease in the separation per-formance. Within 40 minutesthe entire 20 l culture washarvested with a 98% degreeof clarification.

Centrifugation parametersin generalA sample’s degree of separationdepends on the centrifugalforce and the flow rate. As with “traditional” centrifuges,acceleration is determined bythe rotor speed and increasesby the speed squared. Thecontinuous flow speed isadjusted via the peristalticpump’s power. A decrease inthe flow rate improves theseparation performance as aresult of the sample’s prolongedlength of stay in the rotor.

Application tipFluid culture media containinglarge volumes of yeast extractor other complex ingredientstend to produce large amountsof lather. In this case there wasa high production of lather dueto the bacteria’s formation ofgas. However, this did notimpair the separation perfor-mance in the Contifuge.

Other applications from the field of cell harvesting

organism particle size flow rate speed degree of commentsµm ml/min rpm clarification %

Acetobacter 1 – 2 500 15,000 99 difficult to woodii sediment

Methanosarcina barkeri 200 15,000 99 strictly anaerobic

Sulfolobus spec. 2 – 3 440 15,000 99 thermophile

Clostridium 500 15,000 to 99.7 anaerobicacetobutylicum OD578 nm = 3.6

Saccharomyces cerevisiae 3 – 5 500 8,000 98

Insect cells/ 16 200 6,000 n.d. very sensitive cells, Sf9 virus culture nearly complete

separation withhigh vitale

Hela-cells 10 650 9,500 n.d. 90 % vigorous

Animal cells undergone lysis (MKS and RNA virus residue) 400 10,000 95

Other applications

Increase in degree of clarification by decreasing the flow rate

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TECHNICAL DATA/ORDER NUMBERSTECHNICAL DATA/ORDER NUMBERS

Model Contifuge stratos

Drive induction drive without carbon brushesControl microprocessor controlled via Easycontrol IIMin./Max speed (rpm) 300/23,000 adjustable in 10 rpm incrementsMax. RCF x g 50,377 Max. capacity ml 400 ml sediment or 4 x 180 mlBraking/acceleration curves 9/9Programs 9 + 1 pre-cooling programNoise level at max. speed dB (A) <60Run time 0 – 9 hrs 59 mins., continuous operation, alternatively 0-99 hrsTemperature range °C –19 to +40Functions RCF pre-selection, Quick-runRotor recognition automaticImbalance recognition electronic, depending on speed and ideally suited to every rotorConstruction galvanised steel chassis with armoured rotor chamberSafety features lid lock and interlocks (two automatic locks)External dimensions (h/w/d) mm 395 x 640 x595Weight (without rotor) kg 134Power consumption W 1,400 (cooling system approx. 900)Standards manufactured and tested in accordance with EN 61 010-2-020,

EN 50 081-1, EN 50 082-1, no approval required in accordance withUVV VBG 7z

Model Equipment Order no.

Contifuge stratos 230 V, 50/60 Hz 75005283200/208 V, 50/60 Hz 75005285

Titanium continuous flow rotor for 400 ml sediment 75003049Aluminium continuous flow rotor for 400 ml sediment 75003054High performance insert polypropylene 75015339Trolley (h x w x d) 764 x 715 x 590 mm 76000083Peristaltic pump 230 V/50 Hz, max. pump volume 1820 ml x rpm 75003503

can be switched to 120 V/60 Hz, max. pump volume 1820 ml x rpm

Contifuge stratos Refrigerated Highspeed Table Top Centrifuge

Order no. 75003049 75003054

Recommended area of application separation of bacteria, cell harvesting of animal and debris, suspended matter human cells

Material titanium aluminium with composite material coating

Max. sediment volume ml 400 400with high performance insert 50 50Autoclavable indefinitely autoclavable indefinitely autoclavableChemical resistance to a great extent similar to PTFE

corrosion resistantMax. speed rpm 17,000 10,000Max./min. radius cm 7.75/5.00 7.75/5.00with high performance insert 7.75/7.25 7.75/7.25Max. RCF x g 25,040 8,665Acceleration/braking time s 80/60 36/35Optimal flow rate ml x rpm 200 to 600 200 to 600

Rotors and accessories

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Easy – the rotor assemblyFor every particular rotorthere is an adaptor whichhouses the automatic rotorrecognition system. Thisavoids any possibility ofconfusion and guaranteesutmost safety as a result ofappropriate speed restric-tions. Work with the Conti-fuge stratos is similarlystraightforward, as withtraditional centrifuges.

Great – the high performance insertThis polypropylene insertimproves the rotor’ssedimentation capability,particularly for smallparticles and those with alow density, whilst reducingthe sedimentation capacity(50 ml). It has been deve-loped for the separation ofsuspensions with a low solidmaterial content. A typicalarea of application is theseparation of fine matter insuspensions for waterexaminations.

The heart – the rotorContinuous flow rotorsdeveloped by Kendro aredesigned to ensure gentlesedimentation of cells with-out lysis. The titanium rotorhas proven to be ideallysuited to fermenter harves-ting of bacterial cultures. It ischemically inert and isdistingnished by its highmaximum speed. Analuminium rotor is offered asan economical alternative. It is worth emphasising thatfor some applications – e.g.the harvest of human or ani-mal cells – lower speeds aresufficient, and these can beachieved with the aluminiumrotor. As with the titaniumrotor, it is fully autoclavable(20 mins., 121 ºC). It has ahigh quality special coating,the chemical resistance ofwhich is similar to that ofTeflon.

Smart – the integrated pumpTo maintain defined andreproducible flow rates, theuse of a peristaltic pumpwith a pump range of 100 to 900 ml x min-1 is recom-mended. Kendro offers aperistaltic pump which iscontrolled via the centrifuge. A minimum speed of 4000rpm is required to fill andoperate the rotor. Thecentrifuge is equipped withadditional electronics and asocket for the pump controlwhich stops the pump as

Mobile – the trolleyA movable centrifuge table – on which the pump, samplecontainers and a magneticstirrer can be placed –makes the entire systemmobile. It can be transportedto any location.

The trolley constitutes anopen, castor-mounted ca-sing in which sample bottlesand a magnetic stirringsystem can be installed. Twolocks on the front castorsimprove stability during ope-ration. The trolley’s reinforcedmetal construction is verysturdy and virtually free fromvibration.

■ The appropriate adaptoris screwed onto the driveshaft.

■ The rotor is simply put ontop of the adaptor.

■ The centrifuge lid is closed.■ Tubing is fitted to the inlet

and outlet points.

soon as the speed fallsbelow this value. The pumpis also automatically switchedoff during acceleration andbraking. When a constantspeed is achieved, it resumesoperation automatically.

titanium rotor

aluminium rotor

Many people talk about service – Kendro fills this idea with life. You will notice this, for example, in the quality of our advice – which is individually geared towards your wishes,ideas and requirements. Our convincing solutions are exactly tailored to your specific needs.

And we will continue to be there for you after your purchase, as a partner who quickly andcompetently assists in all matters and with any problems. For instance, within the bounds ofa maintenance agreement with a 24-hour service – even at weekends and during bankholidays. Or with other service related matters, e.g. calibration, reference measurement,application advice, repairs or the exchange of spare parts – our commitment helps youquickly and avoids red tape.

The Kendro Service Team: a good feeling to know that we are at your side.

WE ARE ONLY SATISFIED IF YOU AREWE ARE ONLY SATISFIED IF YOU ARE

The fax for all who want to know more … +49/6172/21 166

Would you like to receive information on other centrifuges? If so, please tick theproducts that are of interest or give us a call. We will provide you with detaileddocumentation as quickly as possible.

■■ Microlitre CentrifugesBiofuge® pico/fresco, Biofuge® vito, Biofuge® primo, Biofuge® primo R

■■ Highspeed CentrifugesBiofuge® stratos

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