Consumer Products Study

Disinfectants, Mouthwash Antibacterial Hand Soap, Antibiotics, and Spices

By THE WESTON SCHOLARS!!!!! (and gifted and talented students.)

  

MATERIALS Tape

Wax pencil Petri dish

Cotton swabs Forceps

Nutrient broth Sterile disk

Inoculating wire loop Sterile water

Bunsen burner Nutrient agar plate

All consumer products

Procedure

1. Suspend 23g of nutrient agar powder in 1L purified H2O.

2. Mix and heat solution until powder is dissolved and solution becomes an translucent yellow.

3. Autoclave at 121ºC for 15 minutes.

4. Fill as many petri dishes as possible (half full) with nutrient agar and let them solidify.

Preparing Nutrient Agar Plates

Procedure (cont’d)

5. Dip cotton swab in sterile H2O and take samples from mouth, skin, and environment.

6. Rub the swab on nutrient agar plate.

7. Cover nutrient agar plates and label them.

8. Invert nutrient agar plates and place them into the incubator at 37ºC.

Procedure (cont’d)9. For discontinuous streaking, sterilize wire

loop by flaming until the wire turns bright orange.

10.Cool loop by lightly touching the wire to the nutrient agar.

11.Using the wire loop, take bacteria from the nutrient agar plate and streak 4 times on a new agar plate and flame the loop in between.

12.Cover, label, and tape plates shut. 13.Place, inverted, in the incubator at 37°C.

Discontinuous Streaking

 

Procedure (cont’d)

14. For the continuous streaking, use the same procedure as the discontinuous streaking, except you just streak back and forth until you cover the whole nutrient agar plate.

Continuous Streaking

 

Procedure (cont’d)Re-suspend bacteria

1. Pour 10ml of nutrient broth into the continuously streaked plates.

2. Swab bacteria until all bacteria is suspended in liquid3. Swab bacteria into new plates.4. Dip sterile disk in disinfectants, antibiotics, spices,

mouthwash, or antibacterial hand soap and place in center of plates. Tap gently to secure.

5. Label plates and place them inverted in the incubator. 6. Let plates sit in the incubator over night.7. Measure the zone of inhibition (in millimeters) and make

your conclusions.

Zone of Inhibition

Disinfectants• Ajax• Algacide• America’s Choice Pine Cleaner• Ammonia• 10% Bleach• Clorox• Fantastik-Clear• Fantastik-Purple• Scrubbin’ Bubbles• Soft Scrub• Spic and Span• Tilex• Touch of Glass• Wavicide• White Vinegar• Windex• Xtra Pine• 409

Disinfectants

ResultsDisinfectants Zone of Inhibition

Ajax 25mm

Algacide 19mm

America’s Choice 9mm

Clear Ammonia 0mm

Clorox 30mm

Clear Fantastik 11mm

Purple Fantastik 14mm

Scrubbin’ Bubbles 10mm

Soft Scrub 9mm

Spic and Span 12mm

Tilex 9mm

Touch of Glass 1mm

Wavicide 5mm

White Vinegar 15mm

Windex 1mm

Xtra Pine 10mm

409 23mm

10% Bleach 0mm

Results

Disinfectants Zones of I nhibition

25

19

9

0

30

11

14

109

12

9

1

5

15

1

10

23

00

5

10

15

20

25

30

35

Disinfectants

Zo

ne o

f In

hib

itio

n (

mm

)

Ajax

Algacide

Americas Choice

Clear Ammonia

Clorox

Fantastik-Clear

Fantastik-Purple

Scrubbin Bubbles

Soft Scrub

Spic and Span

Tilex

Touch of Glass

Wavicide

White Vinegar

Windex

Xtra Pine

Four o' Nine

Ten Percent Bleach

Best Disinfectants

#1

#2

#3

Mouthwashes

• Biotene• Cepacol• CVS Brand• Diluted Hydrogen Peroxide• Listerine• Listerine Tartar Control• Plax• Rite Aid Brand• Salt Water • Scope• ShopRite Brand• Tom’s Natural Mouthwash

Results

Mouthwashes Zone of InhibitionBiotene 2mm

Cepacol 12mm

CVS Brand 3mm

Hydrogen Peroxide 18mm

Listerine 2mm

Listerine Tartar Control 2mm

Plax 10mm

Rite Aid Brand 3mm

Salt Water 0mm

Scope 3mm

ShopRite Brand 3mm

Tom’s Natural Mouthwash 0mm

Results

Mouthwash's Zones of Inhibition

2

12

3

18

2 2

10

3

0

3 3

00

2

4

6

8

10

12

14

16

18

20

1

Mouthwashes

Zon

e o

f In

hib

tion

(m

m)

BIOTENE

CEPACOL

CVS

DILUTED HYDROGENPEROXIDELISTERINE

LISTERINE TARTARCONTROLPLAX

RITE AID

SALT WATER

SCOPE

SHOPRITE

TOMS NATURALMOUTHWASH

Best Mouthwashes

#1

#2

#3

Hand Soaps

• Bath & Body Works

• Clean n Smooth

• Ajax

• Palmolive

• Soft Soap

• April Body & Shower

Results

Type of Soap Zone of InhibitionClean & Smooth 4mm

Bodyworks 1mm

Softsoap 17mm

Palmolive 15mm

April Bath & Shower 3mm

AJAX 18mm

Results

Soaps

-1.002.003.004.005.006.007.008.009.00

10.0011.0012.0013.0014.0015.0016.0017.0018.0019.00

Name of Soap

Zo

ne o

f In

hib

itio

n

Best Hand Soaps

#1

#2

#3

Antibiotics on the skin bacteria

• Tetracycline • Rifampin • Chloramphenicol • Kanamycin • Streptomycin• Triple Sulfonides• Polymyxin B• Erythomycin• Cephalothin

Results

Name of Antibiotics Zone of Inhibition Sig. of Zone of Inhibition

Tetracycline 21mm Sensitive

Cephalothin 25mm Sensitive

Rifampin 28mm Sensitive

Chloramphenicol 23mm Sensitive

Kanamycin 18mm Sensitive

Streptomycin 12mm Intermediate

Triple Sulfonides 0mm Resistant

Polymyxin B 10mm Intermediate

Erythomycin 15mm Resistant

Results

Antibiotics

0.005.00

10.0015.0020.0025.0030.00

Name of Antibiotics

Zone

of I

nhib

ition

Best Antibiotics

#1 Rifampin

#2 Caphalothin

#3 Chloramphenicol

Spices• Cinnamon• Paprika• Chili Powder• Onion Powder• Cream of Tartar• Coriander• Mustard Powder• All Spice• Alum• Cumin• Nutmeg• Cajun Seasoning• Curry• Ground Ginger• Garam Marsala• Garlic

ResultsCinnamon 5mm

Paprika 0mm

Chili Powder 3mm

Onion Powder 2mm

Cream of Tartar 9mm

Coriander 0mm

Mustard Powder 4mm

Allspice 6mm

Alum 10mm

Cumin 4mm

Nutmeg 4mm

Cajun Seasoning 2mm

Curry 3mm

Ground Ginger 2mm

Garam Masala 4mm

Garlic 10mm

Spices Zone of Inhibition

ResultsSpices as Disinfectants

5

0

3

2

9

0

4

6

10

4 4

2

3

2

4

10

0

2

4

6

8

10

12

Spices

Zo

ne

of

Inh

ibit

ion

(M

M)

Cinnamon

Paprika

Chili Powder

Onion Powder

Cream of Tartar

Coriander

Mustard Powder

All Spice

Alum

Cumin

Nutmeg

Cajun Seasoning

Curry

Ground Ginger

Garam Marsala

Garlic

Best Spices

#1

#2

#3

Dr. Lee H. LeeDr. Lee H. LeePat PlatnerPat Platner

Shi-Fang HsuShi-Fang Hsu

Tina ChuTina Chu

Alex PeroffAlex Peroff

Ms. EnglishMs. English

Ms. PortasMs. Portas

Mr. & Mrs. WestonMr. & Mrs. Weston

AND AND

Thank you!