Consultation Report on East Main Street Fire Department

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CONSULTATION REPORT for City of Meriden Fire Department 561 Broad Street Meriden, CT 06450 SITE VISITED Fire Station #5 1075 East Main Street Meriden, CT 06450 Submitted By: Savita Trivedi, MS, cm Occupational Hygienist State of Connecticut, Department of Labor Division of Occupational Safety and Health 38 Wolcott Hill Road Wethersfield, CT 06109 Phone: (860) 263-6928 Fax: (860) 263-6940

description

Cosultation report on OSHA problems at East Main Street Fire Department in Meriden.

Transcript of Consultation Report on East Main Street Fire Department

CONSULTATION REPORT

for

City of Meriden Fire Department 561 Broad Street

Meriden CT 06450

SITE VISITED

Fire Station 5 1075 East Main Street Meriden CT 06450

Submitted By

Savita Trivedi MS cm Occupational Hygienist

State of Connecticut Department of Labor Division of Occupational Safety and Health

38 Wolcott Hill Road Wethersfield CT 06109

Phone (860) 263-6928 Fax (860) 263-6940

TABLE OF CONTENTS

SUMMARy 1 PURPOSE 1 CONCLUSIONS 1 RECOMMENDATIONS 2 DISCUSSION 3

NOTICE OF OBLIGATION 4

Attachments A - Report of Hazards Found B - Employer Report of Action Taken 1 C - Safety and Health Program Management 1

Safety and Health Program Management with Employee Involvement 1 Management Leadership and Employee Involvement 2 Worksite Analysis 2 Hazard Prevention and Control 2 Training 2

D - Training Provided by Consultant 1 E - Monitoring Report 1

Visit Number 505263848 Report to the Employer

SUMMARY

PURPOSE

An initial specific industrial hygiene consultation visit was made in response to a request from Mr David Bowen Deputy Chief of the Meriden Fire Department for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station

An opening conference was held on February 3 2010 with Deputy Chief Bowen to discuss the scope of the survey and to reiterate the employers rights and responsibilities especially to correct imminent danger or serious hazards The opening conference was also attended by Chief James Trainor

Following the opening conference the consultant conducted a walk-through of the areas included in the consultation Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

CONCLUSIONS

Monitoring Data Monitoring of several substances commonly evaluated to assess indoor air quality was conducted The sampling strategy utilized included carbon dioxidemiddot carbon monoxide temperature and relative humidity None of the substances evaluated resulted in concentrations exceeding limits found in the State of Connecticut Department of Labor Division of Occupational Safety and Health (CONN-OSHA) regulations The results are detailed in Table I in the Monitoring Report section

Environmental sampling for fungi was alsoconduauid The results Of the sampling did not indicate a problem of fungal amplification in the second floor living quarters In the morning sampling quantities of fungal spores in the first and second floors of the facility were found to be mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found in the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low The results are detailed in Tables II m and IV in the Monitoring Report section

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chanarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellulose materials Stachybottys chanarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pUlmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybottys chanarum and particular health symptoms The results of the swab samples are detailed in Table V in the Monitoring Report section

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Hazards Identified During Consultation During this consultation two hazards related to CONN-OSHA regulations were identified The hazards identified were classified as Serious These hazards were discussed with Deputy Chief Bowen and are detailed in the Report of Hazards Found section Please return a response (B - Employer Report of Action Taken) by June 21 2010 indicating corrective actions that have been taken to protect employees and correct the hazardous conditions

RECOMMENDATIONS

bull Management needs to address the condition causing water incursion in the basement Water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well It is recommended that the source(s) of water incursion be identified and repaired immediately

Once the problem(s) of water incursion are corrected thoroughly inspect water-affected areas for water damage andor fungal growth This includes readily observable structures as well as hidden surfaces behind walls and areas underneath furnishings Porous organic materials that are or may be contaminated should be removed and discarded They are potential reservoirs for microorganisms Disinfection is rarely effective for porous materials that have been subject to water damage Non-porous surfaces may be vacuumed using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) filter andor damp wiped with water and a mild detergent and allowed to dry

bull Source sampling indicated the presence of active fungal growth on walls in the utility area supply room 2 and fire room all located in the basement Sheetrock in these noted areas as well as other areas affected by fungal growth should be removed and discarded The surrounding areas should be evaluated for water damage andor visible fungal growth Based on the amount of material affected it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas Steps should be taken to carefully contain and remove moldy building materials in a manner that will prevent further contamination For information on remediation procedures refer to the remediation guidelines outlined in the OSHA publication entitled A Brief Guide to Mold in the Workplace the Environmental Protection Agency (EPA) guideline entitled Mold Remediation in Schools and Commercial Buildings andor the New York City Department of Health and Mental Hygiene Bureau of Environmental and Occupational Disease Epidemiology publication entitled Guidelines on Assessment and Remediation of Fungi in Indoor Environments

bull Preventive maintenance is vital to controlling bioaerosols in the facility The build-up of dirt and moisture should be prevented by routine cleaning inspection and maintenance activities Immediate attention should be given to problems of moisture incursion in the building including any roof or pipe leaks spills or flood The relative humidity in the building should be maintained below 60 to prevent conditions conducive for microbial growth Good ventilation practices can also help in preventing the build-up of biological particles

bull Some stained ceiling tiles were found on the second floor of the facility Remove and discard any water damaged ceiling tiles They are potential reservoirs for microorganisms Ensure that the underlying cause of water incursion in these areas have been corrected

An effective preventive maintenance program should be maintained for the facilitys ventilation system Operations typically included in a preventive maintenance plan are as follows

changing air fllters on air handling units at regular intervals

Visit Number 505263848 20f4 Summary

conducting routine inspection amp cleaning of air distribution ducts as necessary

replacing damaged insulation

vacuuming amp dusting of ventilation registers regularly to remove surface dust

checking the proper functioning amp conditioning of fans dampers belts baffles ductwork and system balances

Also ensure that outdoor air dampers are open and set to provide an adequate amount of outdoor air to the facility

DISCUSSION - Facility Walk-through

An initial specific industrial hygiene consultation visit was made in response to a request from the deputy chief for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station The fire station consisted of a two-story brick building The facility was reportedly built in 1968

This consultant was informed that there has been an on-going problem of water incursion in the basement level of the facility due to a drainage problem Water reportedly backs into the basement through a drain in the west side stairwell It was believed that the underground drainage pipes may have collapsed causing a water back-up when the water table rises Several areas of the basement have been affected by water incursion resulting in damage to building materials and fungal growth on sheetrock cardboard boxes etc Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

Upon arrival a walkthrough of the facility was conducted The firehouse consisted of a basement first or ground floor and a second floor Access to the basement was from outside of the firehouse The basement was originally designed to serve as an emergency operating center with a conference room several offices supply rooms a storage room kitchen cafeteria shower facilities an emergency generator room furnace room and a utility area Flooring in the basement consisted of concrete and vinyl-type floor tiles The walls were constructed of concrete and sheetrock It was reported that the basement was no longer used for meetings or other activities and that it was primarily used as a storage area Entry into the basement was also required to access the boiler room and emergency generator room both located near the west side stairwell

The first or ground floor of the facility consisted of an apparatus bay and a bathroom The second floor consisted of an office a dormitory a kitchen two sitting rooms - Cinema 1 and Cinema 2 and bathrooms Flooring in the living quarters on the second floor consisted of vinyl-type floor tiles with some area carpets The walls were constructed of concrete block and the ceilings were composed of ceiling tiles Two mechanical rooms were located on the second floor one in the dormitory and one inmiddot the northwest corridor The air handling units for the second floor were located in the mechanical rooms along with an exhaust duct which ran from the basement to the rooftop

On the day of the visit water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area west side corridor and activity room Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well as on some cardboard boxes Also some stained ceiling tiles were found on the second floor of the firehouse The stains were reportedly a result of a problem of condensation on pipes which has since been corrected

At the closing conference the Hazard Descriptions and Recommendations and Monitoring Data sections of this report were discussed with the deputy chief The abatement date for the alleged violations was discussed with management

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1

NOTICE OF OBLIGATION

As you know we are required to notify the State of Connecticut Department of Labor Division of Occupational Safety and Health (enforcement branch of CONN-OSHA) if serious hazards are not corrected within the agreed-upon time Extensions may be granted if you encounter difficulties completing correction within these time frames but we must receive your request for an extension in writing before the correction due date

The following information is required when an extension is requested

1 The item number(s) for which an extension is desired 2 Identify all steps taken to achieve compliance during the agreed upon abatement

period including the date(s) of these steps 3 The additional abatement time necessary in order to achieve compliance 4 The reasons why an extension is necessary (unavailability of material equipment

help etc) 5 Identify all available interim or temporary measures being taken to safeguard

employees from the hazards 6 Any additional infonnation you believe may be-helpful to this office in considering

your request for an extension

(A Request for Extension fonn has been enclosed for your convenience)

Although we are not required to notify CONN-OSHA enforcement branch if other-than-serious hazards are not corrected these hazards could result in injury to your employees Moreover your company would be subject to citations for them in the event of a CONN-OSHA enforcement inspection

Please infonn this office of the corrective steps you have taken and of their dates together with adequate supporting documentation eg drawings or photographs of corrected conditions purchasework orders related to abatement actions air sampling results etc

It is imperative that the appropriate corrective actions are taken to eliminate employee exposure to safety andor health hazards and that this office receive the above required information

Please mail or FAX attachment B - Employer Report of Action Taken completed to show corrective actions taken along with the requested documentation Our FAX number is (860) 263-6940

In the event of a CONN-OSHA enforcement inspection it is important to remember that the Compliance Officer is not legally bound by the consultants advice or by theiconsultants failure to point out a specific hazard You may but are not required to furnish a copy of this report to the Compliance Officer who may use it to determine your good faith efforts toward safety and health and reduceany proposed penalties You are however required to furnish any employee exposure data from this report as required by 31~372-101- 19101020

Savita Trivedi MS crn Occupational Hygienist

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A - Report of Hazards Found

SAFETY AND HEALTH HAZARDS FOUND

Each hazard identified during this consultation is categorized and described and recommendations are given for its correction Hazards are in order of standard number within categories not necessarily in order of importance

Hazards could be in any of the following categories

IMMINENT DANGERS are hazards that can reasonably be expected to cause death or serious physical harm immediately or before this written report is received Any such hazards would have been corrected immediately and no correction dates or space for correction method would appear in A Report of Hazards Found

SERIOUS HAZARDS can cause an accident or health hazard exposure resulting in death or serious physical harm Each such hazard has been assigned a mutually agreed-upon date by which correction is to be completed

OTHER-TI-IAN-SERIOUS HAZARDS lack the potential for causing serious physical harm but could have a direct impact on employee safety and health We encourage you to correct these hazards and notify us of the action taken

REGULATORY HAZARDS reflect violations of CONN-OSHA posting requirements recordkeeping requirements and reporting requirements as found in 29 CFR 1903 and 1904 and adopted by the State of Connecticut No abatement dates have been set but we request notification of their correction

Visit Nwnber 5Q5263848 A-lof2 Report of Hazards Found

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

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Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

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D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

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E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

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ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

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In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

TABLE OF CONTENTS

SUMMARy 1 PURPOSE 1 CONCLUSIONS 1 RECOMMENDATIONS 2 DISCUSSION 3

NOTICE OF OBLIGATION 4

Attachments A - Report of Hazards Found B - Employer Report of Action Taken 1 C - Safety and Health Program Management 1

Safety and Health Program Management with Employee Involvement 1 Management Leadership and Employee Involvement 2 Worksite Analysis 2 Hazard Prevention and Control 2 Training 2

D - Training Provided by Consultant 1 E - Monitoring Report 1

Visit Number 505263848 Report to the Employer

SUMMARY

PURPOSE

An initial specific industrial hygiene consultation visit was made in response to a request from Mr David Bowen Deputy Chief of the Meriden Fire Department for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station

An opening conference was held on February 3 2010 with Deputy Chief Bowen to discuss the scope of the survey and to reiterate the employers rights and responsibilities especially to correct imminent danger or serious hazards The opening conference was also attended by Chief James Trainor

Following the opening conference the consultant conducted a walk-through of the areas included in the consultation Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

CONCLUSIONS

Monitoring Data Monitoring of several substances commonly evaluated to assess indoor air quality was conducted The sampling strategy utilized included carbon dioxidemiddot carbon monoxide temperature and relative humidity None of the substances evaluated resulted in concentrations exceeding limits found in the State of Connecticut Department of Labor Division of Occupational Safety and Health (CONN-OSHA) regulations The results are detailed in Table I in the Monitoring Report section

Environmental sampling for fungi was alsoconduauid The results Of the sampling did not indicate a problem of fungal amplification in the second floor living quarters In the morning sampling quantities of fungal spores in the first and second floors of the facility were found to be mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found in the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low The results are detailed in Tables II m and IV in the Monitoring Report section

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chanarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellulose materials Stachybottys chanarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pUlmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybottys chanarum and particular health symptoms The results of the swab samples are detailed in Table V in the Monitoring Report section

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Hazards Identified During Consultation During this consultation two hazards related to CONN-OSHA regulations were identified The hazards identified were classified as Serious These hazards were discussed with Deputy Chief Bowen and are detailed in the Report of Hazards Found section Please return a response (B - Employer Report of Action Taken) by June 21 2010 indicating corrective actions that have been taken to protect employees and correct the hazardous conditions

RECOMMENDATIONS

bull Management needs to address the condition causing water incursion in the basement Water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well It is recommended that the source(s) of water incursion be identified and repaired immediately

Once the problem(s) of water incursion are corrected thoroughly inspect water-affected areas for water damage andor fungal growth This includes readily observable structures as well as hidden surfaces behind walls and areas underneath furnishings Porous organic materials that are or may be contaminated should be removed and discarded They are potential reservoirs for microorganisms Disinfection is rarely effective for porous materials that have been subject to water damage Non-porous surfaces may be vacuumed using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) filter andor damp wiped with water and a mild detergent and allowed to dry

bull Source sampling indicated the presence of active fungal growth on walls in the utility area supply room 2 and fire room all located in the basement Sheetrock in these noted areas as well as other areas affected by fungal growth should be removed and discarded The surrounding areas should be evaluated for water damage andor visible fungal growth Based on the amount of material affected it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas Steps should be taken to carefully contain and remove moldy building materials in a manner that will prevent further contamination For information on remediation procedures refer to the remediation guidelines outlined in the OSHA publication entitled A Brief Guide to Mold in the Workplace the Environmental Protection Agency (EPA) guideline entitled Mold Remediation in Schools and Commercial Buildings andor the New York City Department of Health and Mental Hygiene Bureau of Environmental and Occupational Disease Epidemiology publication entitled Guidelines on Assessment and Remediation of Fungi in Indoor Environments

bull Preventive maintenance is vital to controlling bioaerosols in the facility The build-up of dirt and moisture should be prevented by routine cleaning inspection and maintenance activities Immediate attention should be given to problems of moisture incursion in the building including any roof or pipe leaks spills or flood The relative humidity in the building should be maintained below 60 to prevent conditions conducive for microbial growth Good ventilation practices can also help in preventing the build-up of biological particles

bull Some stained ceiling tiles were found on the second floor of the facility Remove and discard any water damaged ceiling tiles They are potential reservoirs for microorganisms Ensure that the underlying cause of water incursion in these areas have been corrected

An effective preventive maintenance program should be maintained for the facilitys ventilation system Operations typically included in a preventive maintenance plan are as follows

changing air fllters on air handling units at regular intervals

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conducting routine inspection amp cleaning of air distribution ducts as necessary

replacing damaged insulation

vacuuming amp dusting of ventilation registers regularly to remove surface dust

checking the proper functioning amp conditioning of fans dampers belts baffles ductwork and system balances

Also ensure that outdoor air dampers are open and set to provide an adequate amount of outdoor air to the facility

DISCUSSION - Facility Walk-through

An initial specific industrial hygiene consultation visit was made in response to a request from the deputy chief for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station The fire station consisted of a two-story brick building The facility was reportedly built in 1968

This consultant was informed that there has been an on-going problem of water incursion in the basement level of the facility due to a drainage problem Water reportedly backs into the basement through a drain in the west side stairwell It was believed that the underground drainage pipes may have collapsed causing a water back-up when the water table rises Several areas of the basement have been affected by water incursion resulting in damage to building materials and fungal growth on sheetrock cardboard boxes etc Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

Upon arrival a walkthrough of the facility was conducted The firehouse consisted of a basement first or ground floor and a second floor Access to the basement was from outside of the firehouse The basement was originally designed to serve as an emergency operating center with a conference room several offices supply rooms a storage room kitchen cafeteria shower facilities an emergency generator room furnace room and a utility area Flooring in the basement consisted of concrete and vinyl-type floor tiles The walls were constructed of concrete and sheetrock It was reported that the basement was no longer used for meetings or other activities and that it was primarily used as a storage area Entry into the basement was also required to access the boiler room and emergency generator room both located near the west side stairwell

The first or ground floor of the facility consisted of an apparatus bay and a bathroom The second floor consisted of an office a dormitory a kitchen two sitting rooms - Cinema 1 and Cinema 2 and bathrooms Flooring in the living quarters on the second floor consisted of vinyl-type floor tiles with some area carpets The walls were constructed of concrete block and the ceilings were composed of ceiling tiles Two mechanical rooms were located on the second floor one in the dormitory and one inmiddot the northwest corridor The air handling units for the second floor were located in the mechanical rooms along with an exhaust duct which ran from the basement to the rooftop

On the day of the visit water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area west side corridor and activity room Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well as on some cardboard boxes Also some stained ceiling tiles were found on the second floor of the firehouse The stains were reportedly a result of a problem of condensation on pipes which has since been corrected

At the closing conference the Hazard Descriptions and Recommendations and Monitoring Data sections of this report were discussed with the deputy chief The abatement date for the alleged violations was discussed with management

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1

NOTICE OF OBLIGATION

As you know we are required to notify the State of Connecticut Department of Labor Division of Occupational Safety and Health (enforcement branch of CONN-OSHA) if serious hazards are not corrected within the agreed-upon time Extensions may be granted if you encounter difficulties completing correction within these time frames but we must receive your request for an extension in writing before the correction due date

The following information is required when an extension is requested

1 The item number(s) for which an extension is desired 2 Identify all steps taken to achieve compliance during the agreed upon abatement

period including the date(s) of these steps 3 The additional abatement time necessary in order to achieve compliance 4 The reasons why an extension is necessary (unavailability of material equipment

help etc) 5 Identify all available interim or temporary measures being taken to safeguard

employees from the hazards 6 Any additional infonnation you believe may be-helpful to this office in considering

your request for an extension

(A Request for Extension fonn has been enclosed for your convenience)

Although we are not required to notify CONN-OSHA enforcement branch if other-than-serious hazards are not corrected these hazards could result in injury to your employees Moreover your company would be subject to citations for them in the event of a CONN-OSHA enforcement inspection

Please infonn this office of the corrective steps you have taken and of their dates together with adequate supporting documentation eg drawings or photographs of corrected conditions purchasework orders related to abatement actions air sampling results etc

It is imperative that the appropriate corrective actions are taken to eliminate employee exposure to safety andor health hazards and that this office receive the above required information

Please mail or FAX attachment B - Employer Report of Action Taken completed to show corrective actions taken along with the requested documentation Our FAX number is (860) 263-6940

In the event of a CONN-OSHA enforcement inspection it is important to remember that the Compliance Officer is not legally bound by the consultants advice or by theiconsultants failure to point out a specific hazard You may but are not required to furnish a copy of this report to the Compliance Officer who may use it to determine your good faith efforts toward safety and health and reduceany proposed penalties You are however required to furnish any employee exposure data from this report as required by 31~372-101- 19101020

Savita Trivedi MS crn Occupational Hygienist

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A - Report of Hazards Found

SAFETY AND HEALTH HAZARDS FOUND

Each hazard identified during this consultation is categorized and described and recommendations are given for its correction Hazards are in order of standard number within categories not necessarily in order of importance

Hazards could be in any of the following categories

IMMINENT DANGERS are hazards that can reasonably be expected to cause death or serious physical harm immediately or before this written report is received Any such hazards would have been corrected immediately and no correction dates or space for correction method would appear in A Report of Hazards Found

SERIOUS HAZARDS can cause an accident or health hazard exposure resulting in death or serious physical harm Each such hazard has been assigned a mutually agreed-upon date by which correction is to be completed

OTHER-TI-IAN-SERIOUS HAZARDS lack the potential for causing serious physical harm but could have a direct impact on employee safety and health We encourage you to correct these hazards and notify us of the action taken

REGULATORY HAZARDS reflect violations of CONN-OSHA posting requirements recordkeeping requirements and reporting requirements as found in 29 CFR 1903 and 1904 and adopted by the State of Connecticut No abatement dates have been set but we request notification of their correction

Visit Nwnber 5Q5263848 A-lof2 Report of Hazards Found

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

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D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

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ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

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There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

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AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

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Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

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Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

SUMMARY

PURPOSE

An initial specific industrial hygiene consultation visit was made in response to a request from Mr David Bowen Deputy Chief of the Meriden Fire Department for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station

An opening conference was held on February 3 2010 with Deputy Chief Bowen to discuss the scope of the survey and to reiterate the employers rights and responsibilities especially to correct imminent danger or serious hazards The opening conference was also attended by Chief James Trainor

Following the opening conference the consultant conducted a walk-through of the areas included in the consultation Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

CONCLUSIONS

Monitoring Data Monitoring of several substances commonly evaluated to assess indoor air quality was conducted The sampling strategy utilized included carbon dioxidemiddot carbon monoxide temperature and relative humidity None of the substances evaluated resulted in concentrations exceeding limits found in the State of Connecticut Department of Labor Division of Occupational Safety and Health (CONN-OSHA) regulations The results are detailed in Table I in the Monitoring Report section

Environmental sampling for fungi was alsoconduauid The results Of the sampling did not indicate a problem of fungal amplification in the second floor living quarters In the morning sampling quantities of fungal spores in the first and second floors of the facility were found to be mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found in the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low The results are detailed in Tables II m and IV in the Monitoring Report section

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chanarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellulose materials Stachybottys chanarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pUlmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybottys chanarum and particular health symptoms The results of the swab samples are detailed in Table V in the Monitoring Report section

Visit Number 505263848 lof4 Summary

Hazards Identified During Consultation During this consultation two hazards related to CONN-OSHA regulations were identified The hazards identified were classified as Serious These hazards were discussed with Deputy Chief Bowen and are detailed in the Report of Hazards Found section Please return a response (B - Employer Report of Action Taken) by June 21 2010 indicating corrective actions that have been taken to protect employees and correct the hazardous conditions

RECOMMENDATIONS

bull Management needs to address the condition causing water incursion in the basement Water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well It is recommended that the source(s) of water incursion be identified and repaired immediately

Once the problem(s) of water incursion are corrected thoroughly inspect water-affected areas for water damage andor fungal growth This includes readily observable structures as well as hidden surfaces behind walls and areas underneath furnishings Porous organic materials that are or may be contaminated should be removed and discarded They are potential reservoirs for microorganisms Disinfection is rarely effective for porous materials that have been subject to water damage Non-porous surfaces may be vacuumed using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) filter andor damp wiped with water and a mild detergent and allowed to dry

bull Source sampling indicated the presence of active fungal growth on walls in the utility area supply room 2 and fire room all located in the basement Sheetrock in these noted areas as well as other areas affected by fungal growth should be removed and discarded The surrounding areas should be evaluated for water damage andor visible fungal growth Based on the amount of material affected it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas Steps should be taken to carefully contain and remove moldy building materials in a manner that will prevent further contamination For information on remediation procedures refer to the remediation guidelines outlined in the OSHA publication entitled A Brief Guide to Mold in the Workplace the Environmental Protection Agency (EPA) guideline entitled Mold Remediation in Schools and Commercial Buildings andor the New York City Department of Health and Mental Hygiene Bureau of Environmental and Occupational Disease Epidemiology publication entitled Guidelines on Assessment and Remediation of Fungi in Indoor Environments

bull Preventive maintenance is vital to controlling bioaerosols in the facility The build-up of dirt and moisture should be prevented by routine cleaning inspection and maintenance activities Immediate attention should be given to problems of moisture incursion in the building including any roof or pipe leaks spills or flood The relative humidity in the building should be maintained below 60 to prevent conditions conducive for microbial growth Good ventilation practices can also help in preventing the build-up of biological particles

bull Some stained ceiling tiles were found on the second floor of the facility Remove and discard any water damaged ceiling tiles They are potential reservoirs for microorganisms Ensure that the underlying cause of water incursion in these areas have been corrected

An effective preventive maintenance program should be maintained for the facilitys ventilation system Operations typically included in a preventive maintenance plan are as follows

changing air fllters on air handling units at regular intervals

Visit Number 505263848 20f4 Summary

conducting routine inspection amp cleaning of air distribution ducts as necessary

replacing damaged insulation

vacuuming amp dusting of ventilation registers regularly to remove surface dust

checking the proper functioning amp conditioning of fans dampers belts baffles ductwork and system balances

Also ensure that outdoor air dampers are open and set to provide an adequate amount of outdoor air to the facility

DISCUSSION - Facility Walk-through

An initial specific industrial hygiene consultation visit was made in response to a request from the deputy chief for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station The fire station consisted of a two-story brick building The facility was reportedly built in 1968

This consultant was informed that there has been an on-going problem of water incursion in the basement level of the facility due to a drainage problem Water reportedly backs into the basement through a drain in the west side stairwell It was believed that the underground drainage pipes may have collapsed causing a water back-up when the water table rises Several areas of the basement have been affected by water incursion resulting in damage to building materials and fungal growth on sheetrock cardboard boxes etc Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

Upon arrival a walkthrough of the facility was conducted The firehouse consisted of a basement first or ground floor and a second floor Access to the basement was from outside of the firehouse The basement was originally designed to serve as an emergency operating center with a conference room several offices supply rooms a storage room kitchen cafeteria shower facilities an emergency generator room furnace room and a utility area Flooring in the basement consisted of concrete and vinyl-type floor tiles The walls were constructed of concrete and sheetrock It was reported that the basement was no longer used for meetings or other activities and that it was primarily used as a storage area Entry into the basement was also required to access the boiler room and emergency generator room both located near the west side stairwell

The first or ground floor of the facility consisted of an apparatus bay and a bathroom The second floor consisted of an office a dormitory a kitchen two sitting rooms - Cinema 1 and Cinema 2 and bathrooms Flooring in the living quarters on the second floor consisted of vinyl-type floor tiles with some area carpets The walls were constructed of concrete block and the ceilings were composed of ceiling tiles Two mechanical rooms were located on the second floor one in the dormitory and one inmiddot the northwest corridor The air handling units for the second floor were located in the mechanical rooms along with an exhaust duct which ran from the basement to the rooftop

On the day of the visit water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area west side corridor and activity room Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well as on some cardboard boxes Also some stained ceiling tiles were found on the second floor of the firehouse The stains were reportedly a result of a problem of condensation on pipes which has since been corrected

At the closing conference the Hazard Descriptions and Recommendations and Monitoring Data sections of this report were discussed with the deputy chief The abatement date for the alleged violations was discussed with management

Visit Number 505263848 3 of 4 Summary

1

NOTICE OF OBLIGATION

As you know we are required to notify the State of Connecticut Department of Labor Division of Occupational Safety and Health (enforcement branch of CONN-OSHA) if serious hazards are not corrected within the agreed-upon time Extensions may be granted if you encounter difficulties completing correction within these time frames but we must receive your request for an extension in writing before the correction due date

The following information is required when an extension is requested

1 The item number(s) for which an extension is desired 2 Identify all steps taken to achieve compliance during the agreed upon abatement

period including the date(s) of these steps 3 The additional abatement time necessary in order to achieve compliance 4 The reasons why an extension is necessary (unavailability of material equipment

help etc) 5 Identify all available interim or temporary measures being taken to safeguard

employees from the hazards 6 Any additional infonnation you believe may be-helpful to this office in considering

your request for an extension

(A Request for Extension fonn has been enclosed for your convenience)

Although we are not required to notify CONN-OSHA enforcement branch if other-than-serious hazards are not corrected these hazards could result in injury to your employees Moreover your company would be subject to citations for them in the event of a CONN-OSHA enforcement inspection

Please infonn this office of the corrective steps you have taken and of their dates together with adequate supporting documentation eg drawings or photographs of corrected conditions purchasework orders related to abatement actions air sampling results etc

It is imperative that the appropriate corrective actions are taken to eliminate employee exposure to safety andor health hazards and that this office receive the above required information

Please mail or FAX attachment B - Employer Report of Action Taken completed to show corrective actions taken along with the requested documentation Our FAX number is (860) 263-6940

In the event of a CONN-OSHA enforcement inspection it is important to remember that the Compliance Officer is not legally bound by the consultants advice or by theiconsultants failure to point out a specific hazard You may but are not required to furnish a copy of this report to the Compliance Officer who may use it to determine your good faith efforts toward safety and health and reduceany proposed penalties You are however required to furnish any employee exposure data from this report as required by 31~372-101- 19101020

Savita Trivedi MS crn Occupational Hygienist

Visit Number 505263848 4of4 SllII1II1afy

A - Report of Hazards Found

SAFETY AND HEALTH HAZARDS FOUND

Each hazard identified during this consultation is categorized and described and recommendations are given for its correction Hazards are in order of standard number within categories not necessarily in order of importance

Hazards could be in any of the following categories

IMMINENT DANGERS are hazards that can reasonably be expected to cause death or serious physical harm immediately or before this written report is received Any such hazards would have been corrected immediately and no correction dates or space for correction method would appear in A Report of Hazards Found

SERIOUS HAZARDS can cause an accident or health hazard exposure resulting in death or serious physical harm Each such hazard has been assigned a mutually agreed-upon date by which correction is to be completed

OTHER-TI-IAN-SERIOUS HAZARDS lack the potential for causing serious physical harm but could have a direct impact on employee safety and health We encourage you to correct these hazards and notify us of the action taken

REGULATORY HAZARDS reflect violations of CONN-OSHA posting requirements recordkeeping requirements and reporting requirements as found in 29 CFR 1903 and 1904 and adopted by the State of Connecticut No abatement dates have been set but we request notification of their correction

Visit Nwnber 5Q5263848 A-lof2 Report of Hazards Found

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Hazards Identified During Consultation During this consultation two hazards related to CONN-OSHA regulations were identified The hazards identified were classified as Serious These hazards were discussed with Deputy Chief Bowen and are detailed in the Report of Hazards Found section Please return a response (B - Employer Report of Action Taken) by June 21 2010 indicating corrective actions that have been taken to protect employees and correct the hazardous conditions

RECOMMENDATIONS

bull Management needs to address the condition causing water incursion in the basement Water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well It is recommended that the source(s) of water incursion be identified and repaired immediately

Once the problem(s) of water incursion are corrected thoroughly inspect water-affected areas for water damage andor fungal growth This includes readily observable structures as well as hidden surfaces behind walls and areas underneath furnishings Porous organic materials that are or may be contaminated should be removed and discarded They are potential reservoirs for microorganisms Disinfection is rarely effective for porous materials that have been subject to water damage Non-porous surfaces may be vacuumed using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) filter andor damp wiped with water and a mild detergent and allowed to dry

bull Source sampling indicated the presence of active fungal growth on walls in the utility area supply room 2 and fire room all located in the basement Sheetrock in these noted areas as well as other areas affected by fungal growth should be removed and discarded The surrounding areas should be evaluated for water damage andor visible fungal growth Based on the amount of material affected it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas Steps should be taken to carefully contain and remove moldy building materials in a manner that will prevent further contamination For information on remediation procedures refer to the remediation guidelines outlined in the OSHA publication entitled A Brief Guide to Mold in the Workplace the Environmental Protection Agency (EPA) guideline entitled Mold Remediation in Schools and Commercial Buildings andor the New York City Department of Health and Mental Hygiene Bureau of Environmental and Occupational Disease Epidemiology publication entitled Guidelines on Assessment and Remediation of Fungi in Indoor Environments

bull Preventive maintenance is vital to controlling bioaerosols in the facility The build-up of dirt and moisture should be prevented by routine cleaning inspection and maintenance activities Immediate attention should be given to problems of moisture incursion in the building including any roof or pipe leaks spills or flood The relative humidity in the building should be maintained below 60 to prevent conditions conducive for microbial growth Good ventilation practices can also help in preventing the build-up of biological particles

bull Some stained ceiling tiles were found on the second floor of the facility Remove and discard any water damaged ceiling tiles They are potential reservoirs for microorganisms Ensure that the underlying cause of water incursion in these areas have been corrected

An effective preventive maintenance program should be maintained for the facilitys ventilation system Operations typically included in a preventive maintenance plan are as follows

changing air fllters on air handling units at regular intervals

Visit Number 505263848 20f4 Summary

conducting routine inspection amp cleaning of air distribution ducts as necessary

replacing damaged insulation

vacuuming amp dusting of ventilation registers regularly to remove surface dust

checking the proper functioning amp conditioning of fans dampers belts baffles ductwork and system balances

Also ensure that outdoor air dampers are open and set to provide an adequate amount of outdoor air to the facility

DISCUSSION - Facility Walk-through

An initial specific industrial hygiene consultation visit was made in response to a request from the deputy chief for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station The fire station consisted of a two-story brick building The facility was reportedly built in 1968

This consultant was informed that there has been an on-going problem of water incursion in the basement level of the facility due to a drainage problem Water reportedly backs into the basement through a drain in the west side stairwell It was believed that the underground drainage pipes may have collapsed causing a water back-up when the water table rises Several areas of the basement have been affected by water incursion resulting in damage to building materials and fungal growth on sheetrock cardboard boxes etc Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

Upon arrival a walkthrough of the facility was conducted The firehouse consisted of a basement first or ground floor and a second floor Access to the basement was from outside of the firehouse The basement was originally designed to serve as an emergency operating center with a conference room several offices supply rooms a storage room kitchen cafeteria shower facilities an emergency generator room furnace room and a utility area Flooring in the basement consisted of concrete and vinyl-type floor tiles The walls were constructed of concrete and sheetrock It was reported that the basement was no longer used for meetings or other activities and that it was primarily used as a storage area Entry into the basement was also required to access the boiler room and emergency generator room both located near the west side stairwell

The first or ground floor of the facility consisted of an apparatus bay and a bathroom The second floor consisted of an office a dormitory a kitchen two sitting rooms - Cinema 1 and Cinema 2 and bathrooms Flooring in the living quarters on the second floor consisted of vinyl-type floor tiles with some area carpets The walls were constructed of concrete block and the ceilings were composed of ceiling tiles Two mechanical rooms were located on the second floor one in the dormitory and one inmiddot the northwest corridor The air handling units for the second floor were located in the mechanical rooms along with an exhaust duct which ran from the basement to the rooftop

On the day of the visit water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area west side corridor and activity room Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well as on some cardboard boxes Also some stained ceiling tiles were found on the second floor of the firehouse The stains were reportedly a result of a problem of condensation on pipes which has since been corrected

At the closing conference the Hazard Descriptions and Recommendations and Monitoring Data sections of this report were discussed with the deputy chief The abatement date for the alleged violations was discussed with management

Visit Number 505263848 3 of 4 Summary

1

NOTICE OF OBLIGATION

As you know we are required to notify the State of Connecticut Department of Labor Division of Occupational Safety and Health (enforcement branch of CONN-OSHA) if serious hazards are not corrected within the agreed-upon time Extensions may be granted if you encounter difficulties completing correction within these time frames but we must receive your request for an extension in writing before the correction due date

The following information is required when an extension is requested

1 The item number(s) for which an extension is desired 2 Identify all steps taken to achieve compliance during the agreed upon abatement

period including the date(s) of these steps 3 The additional abatement time necessary in order to achieve compliance 4 The reasons why an extension is necessary (unavailability of material equipment

help etc) 5 Identify all available interim or temporary measures being taken to safeguard

employees from the hazards 6 Any additional infonnation you believe may be-helpful to this office in considering

your request for an extension

(A Request for Extension fonn has been enclosed for your convenience)

Although we are not required to notify CONN-OSHA enforcement branch if other-than-serious hazards are not corrected these hazards could result in injury to your employees Moreover your company would be subject to citations for them in the event of a CONN-OSHA enforcement inspection

Please infonn this office of the corrective steps you have taken and of their dates together with adequate supporting documentation eg drawings or photographs of corrected conditions purchasework orders related to abatement actions air sampling results etc

It is imperative that the appropriate corrective actions are taken to eliminate employee exposure to safety andor health hazards and that this office receive the above required information

Please mail or FAX attachment B - Employer Report of Action Taken completed to show corrective actions taken along with the requested documentation Our FAX number is (860) 263-6940

In the event of a CONN-OSHA enforcement inspection it is important to remember that the Compliance Officer is not legally bound by the consultants advice or by theiconsultants failure to point out a specific hazard You may but are not required to furnish a copy of this report to the Compliance Officer who may use it to determine your good faith efforts toward safety and health and reduceany proposed penalties You are however required to furnish any employee exposure data from this report as required by 31~372-101- 19101020

Savita Trivedi MS crn Occupational Hygienist

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A - Report of Hazards Found

SAFETY AND HEALTH HAZARDS FOUND

Each hazard identified during this consultation is categorized and described and recommendations are given for its correction Hazards are in order of standard number within categories not necessarily in order of importance

Hazards could be in any of the following categories

IMMINENT DANGERS are hazards that can reasonably be expected to cause death or serious physical harm immediately or before this written report is received Any such hazards would have been corrected immediately and no correction dates or space for correction method would appear in A Report of Hazards Found

SERIOUS HAZARDS can cause an accident or health hazard exposure resulting in death or serious physical harm Each such hazard has been assigned a mutually agreed-upon date by which correction is to be completed

OTHER-TI-IAN-SERIOUS HAZARDS lack the potential for causing serious physical harm but could have a direct impact on employee safety and health We encourage you to correct these hazards and notify us of the action taken

REGULATORY HAZARDS reflect violations of CONN-OSHA posting requirements recordkeeping requirements and reporting requirements as found in 29 CFR 1903 and 1904 and adopted by the State of Connecticut No abatement dates have been set but we request notification of their correction

Visit Nwnber 5Q5263848 A-lof2 Report of Hazards Found

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

conducting routine inspection amp cleaning of air distribution ducts as necessary

replacing damaged insulation

vacuuming amp dusting of ventilation registers regularly to remove surface dust

checking the proper functioning amp conditioning of fans dampers belts baffles ductwork and system balances

Also ensure that outdoor air dampers are open and set to provide an adequate amount of outdoor air to the facility

DISCUSSION - Facility Walk-through

An initial specific industrial hygiene consultation visit was made in response to a request from the deputy chief for an evaluation of employee exposure to the possible presence of airborne fungal contaminants in the fire station The fire station consisted of a two-story brick building The facility was reportedly built in 1968

This consultant was informed that there has been an on-going problem of water incursion in the basement level of the facility due to a drainage problem Water reportedly backs into the basement through a drain in the west side stairwell It was believed that the underground drainage pipes may have collapsed causing a water back-up when the water table rises Several areas of the basement have been affected by water incursion resulting in damage to building materials and fungal growth on sheetrock cardboard boxes etc Employees had expressed concerns regarding potential migration of fungal contaminants from the basement level of the facility to the living quarters on the second floor

Upon arrival a walkthrough of the facility was conducted The firehouse consisted of a basement first or ground floor and a second floor Access to the basement was from outside of the firehouse The basement was originally designed to serve as an emergency operating center with a conference room several offices supply rooms a storage room kitchen cafeteria shower facilities an emergency generator room furnace room and a utility area Flooring in the basement consisted of concrete and vinyl-type floor tiles The walls were constructed of concrete and sheetrock It was reported that the basement was no longer used for meetings or other activities and that it was primarily used as a storage area Entry into the basement was also required to access the boiler room and emergency generator room both located near the west side stairwell

The first or ground floor of the facility consisted of an apparatus bay and a bathroom The second floor consisted of an office a dormitory a kitchen two sitting rooms - Cinema 1 and Cinema 2 and bathrooms Flooring in the living quarters on the second floor consisted of vinyl-type floor tiles with some area carpets The walls were constructed of concrete block and the ceilings were composed of ceiling tiles Two mechanical rooms were located on the second floor one in the dormitory and one inmiddot the northwest corridor The air handling units for the second floor were located in the mechanical rooms along with an exhaust duct which ran from the basement to the rooftop

On the day of the visit water was found on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area west side corridor and activity room Signs of water damage and visible fungal growth were observed on walls in several areas of the basement as well as on some cardboard boxes Also some stained ceiling tiles were found on the second floor of the firehouse The stains were reportedly a result of a problem of condensation on pipes which has since been corrected

At the closing conference the Hazard Descriptions and Recommendations and Monitoring Data sections of this report were discussed with the deputy chief The abatement date for the alleged violations was discussed with management

Visit Number 505263848 3 of 4 Summary

1

NOTICE OF OBLIGATION

As you know we are required to notify the State of Connecticut Department of Labor Division of Occupational Safety and Health (enforcement branch of CONN-OSHA) if serious hazards are not corrected within the agreed-upon time Extensions may be granted if you encounter difficulties completing correction within these time frames but we must receive your request for an extension in writing before the correction due date

The following information is required when an extension is requested

1 The item number(s) for which an extension is desired 2 Identify all steps taken to achieve compliance during the agreed upon abatement

period including the date(s) of these steps 3 The additional abatement time necessary in order to achieve compliance 4 The reasons why an extension is necessary (unavailability of material equipment

help etc) 5 Identify all available interim or temporary measures being taken to safeguard

employees from the hazards 6 Any additional infonnation you believe may be-helpful to this office in considering

your request for an extension

(A Request for Extension fonn has been enclosed for your convenience)

Although we are not required to notify CONN-OSHA enforcement branch if other-than-serious hazards are not corrected these hazards could result in injury to your employees Moreover your company would be subject to citations for them in the event of a CONN-OSHA enforcement inspection

Please infonn this office of the corrective steps you have taken and of their dates together with adequate supporting documentation eg drawings or photographs of corrected conditions purchasework orders related to abatement actions air sampling results etc

It is imperative that the appropriate corrective actions are taken to eliminate employee exposure to safety andor health hazards and that this office receive the above required information

Please mail or FAX attachment B - Employer Report of Action Taken completed to show corrective actions taken along with the requested documentation Our FAX number is (860) 263-6940

In the event of a CONN-OSHA enforcement inspection it is important to remember that the Compliance Officer is not legally bound by the consultants advice or by theiconsultants failure to point out a specific hazard You may but are not required to furnish a copy of this report to the Compliance Officer who may use it to determine your good faith efforts toward safety and health and reduceany proposed penalties You are however required to furnish any employee exposure data from this report as required by 31~372-101- 19101020

Savita Trivedi MS crn Occupational Hygienist

Visit Number 505263848 4of4 SllII1II1afy

A - Report of Hazards Found

SAFETY AND HEALTH HAZARDS FOUND

Each hazard identified during this consultation is categorized and described and recommendations are given for its correction Hazards are in order of standard number within categories not necessarily in order of importance

Hazards could be in any of the following categories

IMMINENT DANGERS are hazards that can reasonably be expected to cause death or serious physical harm immediately or before this written report is received Any such hazards would have been corrected immediately and no correction dates or space for correction method would appear in A Report of Hazards Found

SERIOUS HAZARDS can cause an accident or health hazard exposure resulting in death or serious physical harm Each such hazard has been assigned a mutually agreed-upon date by which correction is to be completed

OTHER-TI-IAN-SERIOUS HAZARDS lack the potential for causing serious physical harm but could have a direct impact on employee safety and health We encourage you to correct these hazards and notify us of the action taken

REGULATORY HAZARDS reflect violations of CONN-OSHA posting requirements recordkeeping requirements and reporting requirements as found in 29 CFR 1903 and 1904 and adopted by the State of Connecticut No abatement dates have been set but we request notification of their correction

Visit Nwnber 5Q5263848 A-lof2 Report of Hazards Found

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

NOTICE OF OBLIGATION

As you know we are required to notify the State of Connecticut Department of Labor Division of Occupational Safety and Health (enforcement branch of CONN-OSHA) if serious hazards are not corrected within the agreed-upon time Extensions may be granted if you encounter difficulties completing correction within these time frames but we must receive your request for an extension in writing before the correction due date

The following information is required when an extension is requested

1 The item number(s) for which an extension is desired 2 Identify all steps taken to achieve compliance during the agreed upon abatement

period including the date(s) of these steps 3 The additional abatement time necessary in order to achieve compliance 4 The reasons why an extension is necessary (unavailability of material equipment

help etc) 5 Identify all available interim or temporary measures being taken to safeguard

employees from the hazards 6 Any additional infonnation you believe may be-helpful to this office in considering

your request for an extension

(A Request for Extension fonn has been enclosed for your convenience)

Although we are not required to notify CONN-OSHA enforcement branch if other-than-serious hazards are not corrected these hazards could result in injury to your employees Moreover your company would be subject to citations for them in the event of a CONN-OSHA enforcement inspection

Please infonn this office of the corrective steps you have taken and of their dates together with adequate supporting documentation eg drawings or photographs of corrected conditions purchasework orders related to abatement actions air sampling results etc

It is imperative that the appropriate corrective actions are taken to eliminate employee exposure to safety andor health hazards and that this office receive the above required information

Please mail or FAX attachment B - Employer Report of Action Taken completed to show corrective actions taken along with the requested documentation Our FAX number is (860) 263-6940

In the event of a CONN-OSHA enforcement inspection it is important to remember that the Compliance Officer is not legally bound by the consultants advice or by theiconsultants failure to point out a specific hazard You may but are not required to furnish a copy of this report to the Compliance Officer who may use it to determine your good faith efforts toward safety and health and reduceany proposed penalties You are however required to furnish any employee exposure data from this report as required by 31~372-101- 19101020

Savita Trivedi MS crn Occupational Hygienist

Visit Number 505263848 4of4 SllII1II1afy

A - Report of Hazards Found

SAFETY AND HEALTH HAZARDS FOUND

Each hazard identified during this consultation is categorized and described and recommendations are given for its correction Hazards are in order of standard number within categories not necessarily in order of importance

Hazards could be in any of the following categories

IMMINENT DANGERS are hazards that can reasonably be expected to cause death or serious physical harm immediately or before this written report is received Any such hazards would have been corrected immediately and no correction dates or space for correction method would appear in A Report of Hazards Found

SERIOUS HAZARDS can cause an accident or health hazard exposure resulting in death or serious physical harm Each such hazard has been assigned a mutually agreed-upon date by which correction is to be completed

OTHER-TI-IAN-SERIOUS HAZARDS lack the potential for causing serious physical harm but could have a direct impact on employee safety and health We encourage you to correct these hazards and notify us of the action taken

REGULATORY HAZARDS reflect violations of CONN-OSHA posting requirements recordkeeping requirements and reporting requirements as found in 29 CFR 1903 and 1904 and adopted by the State of Connecticut No abatement dates have been set but we request notification of their correction

Visit Nwnber 5Q5263848 A-lof2 Report of Hazards Found

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

A - Report of Hazards Found

SAFETY AND HEALTH HAZARDS FOUND

Each hazard identified during this consultation is categorized and described and recommendations are given for its correction Hazards are in order of standard number within categories not necessarily in order of importance

Hazards could be in any of the following categories

IMMINENT DANGERS are hazards that can reasonably be expected to cause death or serious physical harm immediately or before this written report is received Any such hazards would have been corrected immediately and no correction dates or space for correction method would appear in A Report of Hazards Found

SERIOUS HAZARDS can cause an accident or health hazard exposure resulting in death or serious physical harm Each such hazard has been assigned a mutually agreed-upon date by which correction is to be completed

OTHER-TI-IAN-SERIOUS HAZARDS lack the potential for causing serious physical harm but could have a direct impact on employee safety and health We encourage you to correct these hazards and notify us of the action taken

REGULATORY HAZARDS reflect violations of CONN-OSHA posting requirements recordkeeping requirements and reporting requirements as found in 29 CFR 1903 and 1904 and adopted by the State of Connecticut No abatement dates have been set but we request notification of their correction

Visit Nwnber 5Q5263848 A-lof2 Report of Hazards Found

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Jjelnl~m~b~ 1 Illl$limce I A H~id8J~ Serious

SJand~(middot 191022(a)(2) CorrectioulluemiddotDate June 21 2010

Condition The floor of a workroom was not maintained so far as possible in a dry condition Water was fOWld on flooring in several areas of the basement including but not limited to the west side stairwell landing emergency generator room boiler room utility area activity room and west side corridor

Location Basement

Potential Effects Sprains strains contusions and fractures from falls illness from bacteria or fungi electrical shock from electrical tools or equipment

Standard 191022(a)(2)

Recommended Action The flooring must be maintained in a clean and so far as possible a dry condition Establish procedures to maintain adequate drainage

Condition The facility was not kept clean to the extent that the nature of the work allowed Visible fungal growth was found on walls in several areas of the basement Swab samples confirmed the presence of active fungal growth on walls in the utility area supply room and fire room

Location Basement

Potential Effects Degradation of building materials from fungi has the potential to create adverse health effects including but not limited to respiratory irritation and allergic-type reactions

Standard 1910141(a)(3)(i)

Reconunended Action Sheetrock with fungal growth should be removed and discarded Any other porous organic material that may be contaminated (Le cardboard boxes papers etc) should also be removed and discarded Nonshyporous surfaces that have or may have been affected by microbial growth should be cleaned using a vacuum cleaner equipped with a high efficiency particulate air (HEPA) fIlter and scrubbing with water and a surfactant Based on the amount of material affected by fungal growth it is recommended that microbial remediation be conducted by properly trained personnel using appropriate personal protective equipment and proper containment to prevent the release of fungi into surrounding areas The condition(s) causing water incursion and fungal growth in the basement must be corrected or the problem my recur

Visit Number 505263848 A - 2 of2 Report of Hazards Found

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

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AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

B - Employer Report of Action Taken

From City of Meriden Consultant Savita Trivedi Fire Department 1075 East Main Street Meriden CT 06450

Visit Number 505263848 Date of Survey February 3 2010

~tem Number 1 Hazar~tType 1SeriousStandard I191022(a)(2)

Corr~tionJ)u~pateIJune 21 2010 DateCorreoted1

Actio~ Taken tD Prevent Rtcuttence

Item NllJIlber 2 Hazard Type SeriousStiuldatd 19l0l4l(a)(3)(i)

lp~tante A ConelaquotionD1lePate June 21 2010 Dat~Corrected

Visit Number 505263848 B-1 of 1 Employer Report of Action Taken

C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

Visit Number 505263848 E 1 of 15 Monitoring Repon

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

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AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

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Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

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In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

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Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

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C - Safety and Health Program Management

The following are the basic elements of an effective employee safety and health program

bull MANAGEMENT LEADERSHIP AND EMPLOYEE INVOLVEMENT assigns safety and health responsibility and authority to supervisors and employees and hold them accountable It includes policy formulation program review and encouragement of employee involvement

bull WORKSITE ANALYSIS identifies current and potential hazards It includes a thorough baseline survey to review work processes and individual potential hazards management of change (to deal with facilities equipment and the physical economic and regulatory environment) job hazard analysis (written safe operating procedures for major tasks) a self-inspection program using checklists to detennine whether facilities and equipment are hazardous and pairing inspectors to facilitate employee training and participation and to increase the possibility that new observers will fmd overlooked conditions a system for reporting hazards accident and incident investigation and analysis of injuries and illnesses

bull HAZARD PREVENTION AND CONTROL Prevention consists of regular maintenance and housekeeping emergency planning and preparation first aid ready access to emergency care when required medical surveillance and at the employers option preventive healthcare (eg group health insurance smoking cessation and wellness programs) Control includes guards enclosures locks protective equipment safe work procedures (the result of job hazard analysis) and administrative placement of personnel so as to minimize hazards

bull TRAINING of all personnel from managers through supervisors to employees about the hazards they may be exposed to and their identification prevention and control Managers and supervisors also need training in program management (eg enforcing rules conducting drills) Training can demonstrate management leadership and facilitate employee involvement

In assessing program effectiveness a consultant looks first at written materials (eg statement of purpose goals and objectives emergency plan) for clarity completeness and currency then for evidence that the written materials have been effectively implemented

Safety and Health Program Management with Employee Involvement

A safe and healthful workplace depends on effective management to involve line workers supervisors and managers in ensuring that hazards are identified and that effective physical and administrative protections are established and maintained

The following observations page may help you avoid the recurrence of the hazards and other fmdings noted during the survey and prevent the occurrence of other hazards

Visit Number 505263848 cmiddot lof2 Safety and Healtb Program Management

Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

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D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

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E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

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into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

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ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

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There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

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AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

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Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

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Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

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Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

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In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

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Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

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Management Leadersbip and Employee Involvement

Employer and employee interviews suggested that management is committed to employee safety and health Lines of communication for safety and health concerns have been established and authority and responsibility for such concerns are defined Management does appear to set an example for safe and healthful behavior

Worksite Analysis

Problems with hazard identification could be alleviated by training employees conducting routine inspections and periodic surveys of the work area Sources of further assistance include insurance companies employer associations and the State of Connecticut Department of Labor-Occupational Safety and Health Division Consultation Program

Hazard Prevention and Control

Work remains to be completed in this category The employer must address the items as referenced in the Report of Hazards Found Section of this report Procedures for correcting newly identified hazards and a system for health and safety rule enforcement and discipline should be established

Training

Employee training should be conducted routinely to insure employees remain aware of health and safety priorities and consistently utilize the best practices for specific tasks The Meriden Fire Department is encouraged to use training materials that are tailored to the needs of its employees

Visit Number 505263848 c -2of2 Safety and Health Program Management

D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

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into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

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TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

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ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

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There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

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Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

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Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

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In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

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Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

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D - Training Provided by Consultant

The consultant provided infonnal training for management regarding indoor air quality The CONN-OSHA Air Contaminants Standard 19101000 the guideline developed by the American Society of Heating Refrigerating AirshyConditioning Engineers Inc (ASHRAE) entitled Ventilation for Acceptable Indoor Air Quality the guideline produced by the National Inlttitute for Occupational Safety and Health (NIOSH) entitled Guidance for Indoor Air Quality Investigations and the OSHA publication entitled Preventing Mold-Related Problems in the Indoor Workplace were discussed The guidelines discuss methods used to evaluate general ventilation in the workplace detail the recommended quantity of fresh air which should be supplied to various facilities identify potential air contaminants which could be found in indoor environments and discuss the assessment of fungal problems in a nonshyindustrial indoor environment

Visit Number 505263848 D 1 of 1 Training by Consultant

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

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into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

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TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

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ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

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There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

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AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

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Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

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In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

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Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

E - Monitoring Report

On February 3 2010 carbon dioxide readings were taken throughout the facility to evaluate whether adequate quantities of fresh outdoor air were being introduced into the building A TSI Q-Trak IAQ Monitor Model 8551 was used to evaluate temperature relative humidity carbon monoxide (CO) and carbon dioxide (COJ levels in the facility The COiCO Analyzer was calibrated before and after use with 1 000 parts per million (ppm) CO2 and 50 ppm CO span gases respectively The results of the sampling are tabulated in Table I

Table LCarbon Dioxide Carbon Monoxide Temperature amp Relative Humidity Air Sampling Results Approximately 3 Feet Above Floor Level

Location of Reading Time

Carbon Dioxide (ppm)

Carbon MonOltide

(ppm) Temperature

(ltgtF)

Relative Humidity

()

Outdoor Ambient 250 pm 462 0 347 613

Apparatus Bay Watch Desk First Floor

1055 am 516 01 630 307

120 pm 500 0 637 294

Dormitory Second Floor

1105 am 553 02 693 252

150 pm middot472 0 698 238

Sitting Room - Cinema 1 Second Floor

1125 am 564 02 709 235

200pm 547 0 707 234

Kitchen Second Floor

1130 am 865 03 712 248

205 pm 640 02 707 239

Sitting Room - Cinema 2 Second Floor

1140 am 683 03 712 248

210pm 576 01 709 232

Office Second Floor

1145 am 548 03 705 240

220pm 480 01 702 235

Basement - Northeast Corner 1155 am 456 0 514 349

Basement - Southwest Corridor 1200 pm 444 0 545 449

(1) = Parts Per Million Pans of Air

The carbon monoxide and carbon dioxide results indicated in Table I are below the respective permissible exposure limits of 35 ppm and 10000 ppm as 8-hour time weighted averages established in CONN-OSHA Standard 19101000 Table Z-1

The following rationale explains why carbon dioxide gas concentrations were monitored to help evaluate indoor air quality It is excerpted from Guidelines for Indoor Air Ouality published by the National Institute for Occupational Safety and Health (NIOSH)

Carbon dioxide (CO is a normal constituent of exhaled breath and if monitored can be used as a screening technique to evaluate whether adequate quantities of fresh outdoor air are being introduced

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into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

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TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

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ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

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There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

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Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

into a building or work area The outdoor ambient concentration of CO2 is nonnally 250-350 ppm Usually the CO2 level is higher inside than outside even in buildings with few complaints about indoor air quality However if indoor CO2 concentrations are more than 1000 ppm (3 to 4 times the outside level) there is probably a problem of inadequate ventilation and complaints such as headaches fatigue and eye and throat irritation are frequently found to be prevalent The CO2 concentration itself is not responsible for the complaints However a high concentration of CO2 may indicate that other contaminants in the building may also be increased and could be responsible for occupant complaints

On the day of the consultation visit carbon dioxide levels in the facility were found to be below 1000 ppm the level below which the comfort criteria is likely to be satisfied

CONN-OSHA does not have standards that regulate temperature or humidity in a work area however the OSHA Technical Manual provides engineering and administrative guidance to prevent or alleviate indoor air quality problems Recommendations for air treatment include humidity control in the range of 20-60 and temperature control in the range of 68-76 degrees Fahrenheit Another source of guidance is the American Society of Heating Refrigerating and Air Conditioning Engineers (ASHRAE) The ASHRAE Standard entitled Thennal Environmental Conditions for Human Occupancy recommends that indoor temperature ranges be based on a number of factors including but not limited to clothing insulation and activity levels In workplaces where activity levels and clothing worn are similar to most office environments and the outdoor environment is cool ASHRAE recommends a temperature range of approximately 68 to 78 degrees Fahrenheit Under similar conditions when the outdoor environment is warm ASHRAE recommends a temperature range of approximately 75 to 82 degrees Fahrenheit These temperature ranges are based on relative humidity levels within recommended ranges

Temperature measurements taken in the living quarters on the second floor of the facility were found to be within the general recommended ranges noted above The relative humidity was below 30 the level below which employees may feel discomfort from dryness However due to the associated risks of microbiological growth humidification was not recommended

On the day of the visit temperatures measurements on the second floor of the firehouse ranged from 693 - 712 degrees Fahrenheit and the relative humidity ranged from 232 - 252

Visit Number 505263848 E 20flS Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

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Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Air sampling was perfonned on February 3 2010 to evaluate the levels of fungi present in the facility Samples were collected on standard petri dishes using a Graseby-Andersen Model 10-709 N-6-1 AFCM single stage viable impactor sampler factory calibrated to an approximate flow rate of 283 liters of air per minute Samples were collected for three-minute time periods on Malt Extract Agar Air samples were collected from inside and outside of the facility Two sets of samples were collected from the first and second floors of the facility one in the morning and one in the afternoon In addition three samples were collected mid-day from the basement The samples were sent to the Wisconsin Occupational Health Laboratory for analysis

Analysis was performed by enumeration and classification of incubated colonies Biological materials which are unable to produce spores are counted and included as non-sporulating fungi The results of analyses are expressed in tenns of colony forming units per cubic meter of air (CFUM3

) The results of the morning mid-day and afternoon samples are listed in Tables II III and IV respectively

Additionally four swab samples were taken from surfaces suspected to have fungal growth The samples were submitted to the Wisconsin Occupational Health Laboratory for fungal analysis The samples were analyzed by standard mycology procedures Wipes are washed with sterile water dilutions are made with sterile water and aliquots are cultured on an agar Fungi are identified by microscopic and macroscopic examination The results of the swab samples are listed in Table V

Visit Number 505263848 E - 3 of 15 MOnitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

TahlenFwgalSpeciitiODandQruintitation Momingo$amples

Total Colony FUngal ID amp Concentration Formiilg Units per mColtmy FomJfug Uiuts perCllbic Cubic MeteiltotAk MererofmiddotAir

(CFPI~3)S~ljngJQclatiQn (OFQramp13)MediUirllI$~

Malt Extract Agar 12 12 Basidiomycete Outdoor Ambient

24 Cladosporium species Watch Desk Area 12 Penicillium species

Apparatus Bay Malt Extract Agar 72 12 Oidiodendron species 12 Stachybotrys chartarum 12 Non-sporulatiru fungi

47 Rhodotorula species 24 Penicillium species

Area Between Rear Door 12 Non-sporulating fungi and Gear Rack

Malt Extract Agar 131 12 Cladosporium species

Apparatus Bay 12 Yeast 12 Epicoccum nigrum

12 ASDendllus versicolor

Dormitory - Outside Mechanical Room Malt Extract Agar 47 47 Non-sporulating fungi

Second Floor

12 Cladosporium species Outside Mechanical Room

Northwest Corridor 36 12 Oidiodenrlron species

Second Floor Malt Extract Agar

12 Miscellaneous unidentified

24 Non-sporulating fungi 24 Rhodotorula species

Sitting Room (Cinema 2) Malt Extract Agar 12 Cladosporium species Second Floor

84 12 aerobic actinomycetes

12 Yeast

Visit Number 505263848 E - 4 of 15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Ta17leU[FungllSpeciatiOJland Quanutation Late MdmIng-MidDay Samples

~

SQll1pling LocentaJiQ~

Room in Northeast Corner Basement

Corridor Outside Generator amp Boiler Rooms

Basement

Southwest Corridor - Near Womens Dormitory

Basement

M~cijtitll J1sed

Malt Extract Agar

Malt Extract Agar

Malt Extract Agar

Total Colony FormingUnitsper

bullCUbkMetermiddotofAlr (CVUlMl)

554

291

421

FungalID ampC(jnc~ntrati()n

in Colqriy Forming UnitsperClibic Meter ofAir

(CFUM3)

400 Cladosporium species 35 Aspergillus sydowii

35 Non-sporulating fungi 24 Penicillium species

12 Puciola spinosa 12 Yeast

12 Aspergillus versicolor 12 Aureobasidium puUularu

12 Oidiodendron species

160 Cladosporium species 47 Non-sporulating fungi

24 Penicillium species 24 Basidiomycete

12 Oidiodendron species 12 Yeast

12 Aspersdllus versicolor

220 Malbranchea species 130 Non-sporulating fungi 71 Cladosporium species

Visit Number 505263848 E - 5 of 15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

ra~le IV FungalSpeciatiQnltllld QuiUltitation A(ternoonSamples

San1J~lipg LQcatiQXl MediUrnJil~d

Total Colony FdrmingUnitsper Cubic Meter ofAir

(CfUM1)

Fungal II) amp Concentration in Colony Fornrlng iJnits per Oubm

Meter ofAir (I ~CFUJrvt)

Outdoor Ambient MaIt Extract Agar 964

580 Penicillium species 200 Cladosporium species

160 Aspergillus sydowii 12 Basidiomycete

12 Botrytis species

Watch Desk Area Apparatus Bay

MaIt Extract Agar 24 12 Penicillium species 12 Stachybotrys chartarum

Area Between Rear Door and Gear Rack Apparams Bay

MaIt Extract Agar 60 24 Penicillium species

12 Cladosporium species 12 Alternaria species

12 Aerobic actinomvcetes

Dormitory Outside MechanicaI Room

Second Floor MaIt Extract Agar

36 24 Cladosporium species 12 Non-sporulating fungi

Northwest Corridor Outside Mechanical Room

Second Floor MaIt Extract Agar

lt 12 No fungi detected

Sitting Room (Cinema 2) Second Floor

MaIt Extract Agar 47 35 Non-sporulating fungi

12 Yeast

E - 6 of 15 Monitoring Report Visit Number 505263848

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

middot1 lt

Table V~Wipe sample AtuUysis for Fungi

Fungali~PampConcentlregon SanlpliDgLQcatiQl in CplQITY FoimiJlgJlnitampperSQWleInC)L(CFllTlirt~5

90000 Cladosporium species Black dust-like material on wall 22000 Miscellaneous unidentified

Utility Area across from Generator Room 2000 Aspergillus sydowii Basement 100 Acremonium species

Total 114100

630000 Stachybotrys chartarum Black dust-like material on wall 40000 Penicillium species

Supply Room 2 2000 Uloc1adium species Basement

Total 672000

540000 Stachybotrys chartarum Black dust-like material on wall 200000 Aspergillus versicolor

Fire Room 1200 Cladosporium species Basement

Total 741200

Black material on concrete wall Mechanical Room off of Dormitory No Fungi Detected lt 100

Second Floor

Blank Wipe No Fungi Detected lt100

Stachybouys species seen on direct microscopic examination not isolated in culture

Visit Number 505263848 E 7 of 15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

Visit Number 505263848 E - 8 of 15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

There are no established pennissible exposure limits (PELs) for microbial levels in bulk or air samples under the State of Connecticut Department of Labor Occupational Safety and Health Division (CONN-OSHA) regulations There are also no established threshold limit values for microbials established by the American Conference of Governmental Industrial Hygienists as recommended guidelines Some quantitative values have been proposed However there are many variables which affect microbial concentrations in indoor air Spores of fungi are almost always present however the types and quantities of fungi vary with the time of day weather season and geographical location Typically the quantities of fungal spores will be greater outdoors than indoors Also similar types of fungi are found indoors as are found outdoors It should be kept in mind that air sampling for fungi in itself may not be used to predict potential adverse health responses in an indoor environment It is usually much easier to carry out remedial actions than to prove that a microbial agent may be responsible for a building-related illness

Current reference sources indicate that indoor sample fungal genera and levels should be equal to or less than outdoor air fungal levels Different genera or increased levels of fungi present indoors as compared to outdoors may indicate fungal growth indoors It should be noted that fungi are ubiquitous in the environment Low quantities of fungi may not be significant especially in bulk and wipe samples

On the day of the visit in the morning sampling quantities of fungal spores in the first and second floors of the facility were mostly of the same order of magnitude as the background level found in the ambient outdoor air and were lower than quantities found in the basement In the afternoon sampling quantities of fungal spores in the indoor locations sampled were lower than the background level found in the ambient outdoor air The predominant fungi found outdoors Penicillium and Cladosporium were amongst the fungi found indoors Certain genera were found on the first and second floors of the facility which were not detected in the outdoor samples However the concentrations of these genera were low These included Oidiodentiron Stachybotrys chartarum Rhodotorula Epicoccum nigrum Aspergillus versicolor Alternaria Yeast and aerobic actinomycetes

In addition swab samples were taken from four surfaces suspected to have fungal growth Fungal growth was not detected in the sample collected from the wall of the second floor mechanical room Active fungal growth was found on samples collected from walls in the utility area supply room 2 and fire room located in the basement It should be noted that the predominant species found on two of the swab samples was Stachybotrys chartarum The growth of Stachybotrys chartarum generally occurs on wet building materials that contain high cellulose content and low nitrogen content This fungi is usually recovered from chronically wetted cellUlose materials Stachyborrys chartarum is known to produce a mycotoxin under certain conditions In recent years exposure to Stachybotrys chartarum has been linked to acute idiopathic pulmonary hemorrhage among infants The Centers for Disease Control (CDC) however has published a clarification stating that at present there is no test that proves an association between Stachybotrys chartarum and particular health symptoms

Management should periodically inspect the building for signs ofmoisrure leaks or spills Respond promptly to any problems noted by correcting causes of any moisture incursion removing and replacing any water damaged porous materials and by cleaning non-porous surfaces with detergent and water

Refer to the Fungal Glossary on pages 9 - 15 of this section for further infonnation regarding the fungi listed in Tables II V

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Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Fungal Glossary

NOTE Molds have the potential to be irritants and allergens In high concentrations or for s~eptible populations they can produce symptoms of watery eyes runny noses scratchy throats headaches and malaise

Absidia- This genera is cosmopolitan and ubiquitous in nature as common environmental contaminants They are found in plant debris and soil as well as being isolated from foods and indoor air environments They often cause food spoilage This genera currently has 21 species

Acremonium - It is a fungus that is ubiquitous in nature and is associated with decaying plant materials and foods as well as soils It is commonly associated with cellulose-based building materials suffering from chronic wet conditions

Acrodontium sahrumeum - This is a hyphomycete common in soil and air considered a mitosporic fungi with ascomycetous or basidiomycetous sexual stages It is considered as an environmental contaminant

Actinomycetes - Aerobic actinomycetes are ubiquitous bacteria found in soil plant surfaces and decaying vegetation This bacteria may be associated with hypersensitivity pneumonitis

AHernaria - This is a very common allergen It is often found in outdoor samples It can be isolated from many kinds of plants foodstuffs soils textiles decaying wood compost and weed pulp It can often be found in carpets window frames and on horizontal surfaces in building interiors Alternaria is found to release spores in dry air and certain species have been identified as causes of allergenic rhinitis and asthma

Aphauocladimp Species of this fungi are common but comprise a small proportion of the fungal biota The species of this mold AJgthanocladiurn allnw1 has been isolated from soil and plant matter

Arthrinium - This is a cosmopolitan fIlamentous fungi isolated from plant debris and soil Currently there have not been any reported infections from this genus

Ascomyoota- This is a taxonomic division containing yeasts and sac fungi representing the largest division of fungi containing 50000 species which accounts for about 75 of all known fungi All species within this division produce ascospores

Aspergillus - There are more than one hundred species of this mold including candidus ilaYus fumizatus ~laucus nidulans ~ and Q~hraceus sydowii terreus JJSlUS and versicQlor It may act as an opportunistic pathogen capable of infecting lungs ears and eyes Certain species have been associated with alveolitis Many types of these molds commonly grow in indoor environments

Asper~illus candidus - is found in warm soils grain and in the secondary decay of vegetation Asper2i1lus ~ - produces aflatoxin a potent mycotoxin and human carcinogen It may cause aspergillosis in susceptible individuals Aspergillus funrigatus - found on water-damaged materials and organic rich substrates in buildings Asper~illus glaucus found in decaying food products fruit juices grains nuts milled rice and various foodstuffs

Visit Number 505263848 E - 9 of 15 Monitoring Repon

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

AsperilJus nidulans - commonly found in spices and other foods It occurs on deteriorated cellulose materials Can cause aspergillosis It may produce toxins including the potent carcinogen and mutagen sterimatocystin a precursor to aflatoxin Asperg-illus ~ - isolated from house dust soil plant litter dried fruits seeds and different kinds of untreated textiles (jute hemp) Asperg-ilJus opoundbraceus - sometimes encountered in building enviromnents It produces Ochratoxin A which is primarily a nephrotoxic compound Asperg-illus restrktus - are distributed widely and found in soil and plant material Asperillus sydowji - is reported to be only occasionally pathogenic Asper~illus terreus - is found in warm soil an din grains straw cotton and decomposing vegetation It produces mivinolin a metabolite which is an inhibitor of cholesterol biosynthesis It has been used in the treatment of a large number of patients with hypertension Asperiil1us lSIDS - is normally present in low numbers in the soil in tropical to subtropical areas It is relatively uncommon in other habitats Aspergillus versicolor- is an indicator organism of moisture problems in houses with a characteristic musty odor It is frequently isolated from water damaged building materials or materials subjected to long-term humidification It is also associated with mucosal irritation such as eye nose and throat irritation

Aureobasidium - The species of this mold Aureobasidium pullulans is a worldwide common soil isolate It colonizes on the surfaces of leaves in the fall It requires elevated humidity or moisture

Basidiomycetes - This term is used taxonomically to represent a division of fungi more commonly known as club fungi jelly fungi rusts smuts bracket fungi mushrooms and puffballs accounting for about 25000 species Research has demonstrated that many basidiomycetes are potential allergens

Beauveria - It occurs naturally in some soils and on some plants and its growth is favored by warm moist weather conditions Insects are commonly the host of this fungus

Bipolaris - It is widespread found in grasses soil and plant materiaL

Botrytis - This mold is found on plant materials

Chaetornium- Is an ascomycete which is adept at digesting cellulose paper cotton and cellulose-made materials Under moist conditions this genera can rapidly decompose these materials It is a common fungus found in soils dung decaying organic matter seeds and other cellulose containing material It is common on many indoor surfaces and is prevalent within carpets cardboard paper and sheetrock It is not a common human pathogen

Clnysonilia - This genus is a mold which lacks a known sexual state and has been organized into the Fungi Imperfecti Neurospora sitQphila is a teleomorph of this species

Cluysosporium - This is a keratinophilic fllamentous fungus commonly isolated from soil plant material dung and birds It lives on remains of hairs and feathers in soil A common contaminant it is occasionally isolated from human infections

Visit Number 505263848 E-lOof15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Cladosporium - This is the most commonly encountered mold in both indoor and outdoor air This common allergen is ubiquitous and is most frequently encountered in close proximity to beech trees soil paints window frames and low drainage environments Certain species of Cladosporium have been identified as causes of allergic rhinitis and asthma

Coelomycetes - A group of fungi found outdoors as a plant pathogen and in the soil

CllIlIIinJbamella - This genus currently contains seven species only one is known as a human or animal pathogen This is a filamentous fungi found in plant and soil materials It is known as a common contaminant and is also known as an opportunistic fungus which may cause infections in immunocompromised hosts

Cl1rvularia - It is a mold which is reported to be allergenic

Cylindrocarpon - It occurs in soil as a saprophyte or weak pathogen associated with roots of many herbaceous and woody plants

Drecbslera - This is an older taxonomic classification for fungi belonging to the Fungi Imperfecti and is compared to Bipolaris and Exserohilum genera Pyrenophora is a teleomorph of this genus

EWOodontium - This is a corrunon fungi There are approximately 6 species in this genus It is commonly isolated from paper textiles and painted walls

Epicoccum bull The species of this mold known as ni~ is a common allergen found in plant materials soil moldy paper and pulp It occasionally grows on water-damaged drywall or wood products No mycotoxin production bas been reported willi this species

Exophiala - It can be found on painted walls particularly in humid places such as showers Some substances that seem to be entirely wliikely to support fungal growth may be found to be well colonized by these organisms ExqphiaJa spedes are found jn syrupy solutions ofpoJyvinyl alcohol

Fusarium - A common soil fungus this genus is fOWld on a wide range of plants Some species may produce toxins It is a potential pathogen

Geomyces - This is a mold which lacks a sexual state and is organized into the Fungi Imperfecti Chrysosporium and Sporotrichum are obsolete synonyms used for this mold

Geotricbium ~andidum bull This is a cosmopolitan fungi found in soil water cereal fruit bread milk products paper and textiles It is the causative agent in geotrichosis of the pulmonary bronchial oral gastrointestinal and cutaneous tissues

Gliocladium - It is commonly found organism and is considered a mold allergen

Helminthosporium - This fungus is known as a mold of grasses It is compared structurally with Bipolaris and Drechslera It is not known as a human pathogen

HonnographieDa - This organism can be found in soil leaves compost and air It is common in the environment

Visit Number 505263848 E - 11 of 15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Hyalodendron - Mostly fmUld on wood This genus is similar to Cladosporium species except for a lack of pigmentation

Hyphae - This is a long threadlike or filamentous structure of fungi which can either be coenocytic (tubular) or septate having filaments divided by cellular cross walls

Malbranchea - There is widespread distribution of this mold in soil and decaying vegetation This species has not been found to be associated with any illnesses

Memnoniella - Closely related to Stachybotrys Worldwide distribution in plant and soil Reported to produce several mycotoxins It can be recovered from chronically wet cellulose containing materials

Mucor - This genus contains several species It is a filamentous fungi found in soil plants decaying fruits and vegetables It is also one ofthe most frequent molds found in house dust They are ubiquitous in nature and are a common laboratory contaminant Mucor is also known as one of the causative agents in zygomycosis Of muconnycosls

Mycotypha - Species of this fungus comprise a very small proportion of the fungal biota Natural habitat is soil

Myxomycetes- This taxonomic division contains the slime molds Recently this group was taken out of the Kingdom ofFungi and reorganized into the Kingdom of Protista Slime molds can be unicellular colonial and multicellular

Neurospora - This organism is known as an ascomycetous mold

Niamprospora - It is reported to be allergenic It is widely distributed in soil decaying plants and seeds

Non-sporulating fungi - This tenn refers to fungi which do not produce spores nor exhibit a sexual stage during culture on agar plates

Oidiodendron - Widespread occurring in humus layers offorest soils decaying wood and bark The species cerealis was recovered from a patient diagnosed with neurodennatitis nuchae

Paecilomyces - This is a toxigenic fungus and a very common thennotolerant fungus from soil air polluted water pulp paper and various plant materials It is commonly found in the soil and dust but less frequently in air

Penicillium - There are over two hundred species of this organism It is found on concrete wall surfaces It has been recovered from carpets and from drywall surfaces that have been subject to flooding Some species may be toxigenic

Periconia - This mold is widely distributed in soils and can be found on grasses and grains

Pestalotiopsis- This is a mold without a known sexual stage and is classified in the Fungi Imperfecti

Visit Number 505263848 E - 120flS Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Pbaeoannellomyces - Resides mainly in the soil in tropical and subtropical environments Agent oftinea nigra palmaris producing dark lesions on the palm ofone hand or both and sometimes the so les of the feet which clinically resemble those of malignant melanoma and from which it must be differentiated Occasionally isolated from saltwater fish

Pboma - A common indoor allergen Found on various plant parts and in soil It can grow extensively on painted walls particularly in humid places

Pitbomyces - This mold is found in plant materials and soil It has also been associated with decaying wood

Polythrincium - Are found as conidiospores in dense clusters on host leaves and is considered parasitic on leaves

Pseudogymnoascus - This is the teleomorph (sexual stage) of Geomyces Geomyces used to be called Chrysosporium species

Puciola spinosa - This is an environmental mold which is not commonly found

Rbinocladiella - This genus name has been replaced by Sporothrix These fungi are found worldwide and have been isolated from soil living and decomposing plants woods and peat moss One species SporQthrix schenckii is an occasional cause ofhuman infections

Rhizopus ~ This mold commonly occurs in soil and plant material

Rhodotorgla - Species of this mold are reddish yeasts which are typically found in moist environments such as carpeting cooling coils and drain pans This yeast has been reported to be allergenic

Scedosporium - This is a filamentous fungus which occasionally causes infections in humans Immunocompromised individuals or healthy individuals may post traumatically become infected

Scopulariopsis - Commonly recovered from building dust and drywall surfaces suffering from chronic wetness It is also recovered from various food products It is a potential pathogenic fungus and may cause infection of the skin and even more common infection of the nails

Spegazzinia - This species belongs to the Fungi Imperfecti as it has no observable sexual stage Currently there is no information about health effects or toxicity

Sporobolomyces - These species are yeasts which are mainly associated with wet and damp conditions They are reported to be allergenic

Sporotricbum - It is reported to be allergenic It is commonly found on decaying plant matter wet and rotting wood and in landscaping mulch

Stacbybotrys - There are several strains of this fungus In particular StachybQtrys chartarum (also known as atra or altemans) growth requires wet building materials that contain high cellulose content and low nitrogen content The chartarum species is a toxigenic mold that is usually but not always recovered from chronically wetted cellulose materials

Visit Number 505263848 E - 13 of 15 Monitoring Repon

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

In recent years Stachybottys chartarwn has been linked to health problems in occupants of contaminated buildings The New York City Department of Health was a sponsor of the article entitled Guidelines On Assessment and Remediation ofStachybotrys atJ in Indoor Environments which was published in 1994 The article describes recommended abatement guidelines for the removal of Stachybotrys aim from indoor environments

The Centers for Disease Control (CDC) however released information about this mold on March 9 2000 in a document entitled Questions and Answers on Stachybotrys chartarum and other molds n The CDC indicated that liTo date a possible association between acute idiopathic pulmonary hemorrhage among infants and StachybotIys chartarum (Stachybotrys m) has not been proved Further studies are needed to determine what causes acute idiopathic hemorrhage and At present there is no test that proves an association between Stachybotrys chartarum (Stachybotrysmm) and particular health symptoms

Stemphylium - This fungus is ubiquitous in nature It is found in soil wood and decaying vegetation

Streptomyces - This genus has been associated with odorous microbial volatile organic compounds

Syncephalastrum - This is a mold which is commonly isolated in soil It can cause respiratory infections

Tetraploa - This is a mold which lacks a sexual state and is classified among the Fungi Imperfecti

Torula - This is a mold which lacks a known sexual state and is classified among the Fungi Imperfecti It has a dark walled structure (demitiaceous)

Torulomyces la2ena - This species is a common soil fungus It grows under wet conditions and may produce pungent moldy odors

Trichoderma - This is a common soil fungi These species grow under wet conditions and may produce pungent moldy odors

Tritirachium- This is fungi which is widespread and found in decaying vegetation and in the soil It is a known insect pathogen and reported to be allergenic

UlocJadium - This fungus is a high water activity organism It is recovered from smfaces of plants and papers It is not known to be toxigenic

UstilagQ -It is a yeast that inhabits the soil and plant materials Its association with human disease is unclear

Yirticillium M It is commonly found in soil and decaying plant matter and is known to cause plant diseases

Wallemia - This fungus prefers conditions of low moisture and is found in house dust soil and dry foodstuffs

Yeast - These are high water activity microorganisms which can be found in rooms damaged by water Yeasts occur in indoor air from damp indoor sources such as carpets and HVAC units In settled dusts yeasts are exceedingly common The yeast Cmdida is very common in outdoor air especially after rainfall

Visit Number 505263848 E - 140flS Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report

Note This Fungal Glossary was developed with the assistance from the Wisconsin Occupational Health Laboratory

Visit Number 505263848 E-150f15 Monitoring Report