FINAL TECHNICAL REPORT

Construction of an Integrated Database on Biosafety and use of GMOs in India

Principal Investigator : Dr. T. R. Sharma

Coordinator : Prof. K.R. Koundal

Co- Investigators:Dr. K. C. BansalDr. N. K. SinghDr. Kishore Gaikwad

National Research Center on Plant Biotechnology

IARI, New Delhi-110012

www.nrcpb.org Email: trsharma@nrcpb.org

•Construction and Updation of the Database•Retrieval, storage and management of data on biosafety of GMOs•To make GM Crops database public through a dedicated Web site •To impart training to the Scientists and Technical Staff on Biosafety web resources

Objectives of the Project

Relational Diagram Showing Relationship Among Different Tables

WEB-SERVER

(SUN ULTRA10)

COMPUTE-SERVER

(SUN V240)

Enter query Authentication

Outputs

US

ER

INT

ER

FA

CE

Gene

Primary Database

(Transgenics)

Primary Database(GM_Pub)

Promoter

Crop Env_haz

Health_haz

Soil_micr

Develo-per

QUERY INTERFACE

Inputs

MID

DLE

WA

RE

MySQL

APPL-SERVERJSP ENGINE

HTML – DHTML -FLASH

JAVA - JDBCResults

Query

MySQLMySQL

Basic Flow Chart of the NRCPB Database

www.nrcpb.org GM Crop Database

www.nrcpb.orgClick here to

access GM crop database

Availability of Database on WWW

GM Crop Database Home Page

GM Crop Search Database Interface

GM Crop Search Database Interface

Transgenic Line Query Interface

Transgenic Line Query Interface

Result Page for the Query Submitted

Transgene-Crop-Promoter Query Interface

Result Page for the Query Submitted

Promoter Info Query Interface

Result Page for the Query Submitted

Developer Institution Query Interface

Result Page for the Query Submitted

CropNumber of transgenic line

Number of genes

Number of promoter Developer

Cotton 137* 4** 1*** 20

Rice 4^ 5^^ 2^^^ 1

* Brinjal - 1% 1*** 1

* Tomato - 1# 1*** 1

* Potato - 2& 2 && 1

* Chickpea - 1+ 1*** 1

* Corn - 3^^^^ - -

Current status of information stored in transgenics database

* 58 Bt cotton hybrids approved for commercial planting in India till date, rest are released for field trials in different zones.

** (cry1Ac,cry1Ab,cry2Ab,Vip3A), *** (CaMV 35S)^ (SGR1, SGR2, LLRICE06, LLRICE62), ^^ (Psy, crtI, lyc, Zea mays psy, Bar)^^^ (Ubi-1 promoter, GT1 promoter), ^^^^ (cry1F,cry1X, CP4EPSPS)# (Rep gene)& (Ama1, RGA2), && (CaMV 35S, GBSS)+ (Cry1Aa3), % (Cry1Ab)* Transgenic crops under development and field trials

GM Publication Query Interface

Result Page for the Query Submitted

Result Page for the Query Submitted

Result Page for the Query Submitted

Year

Biosafety & IPR

Issues

Food &

Feed

Risks & Benefit

s

Environment & Health

Concern

Bt Cotton RiceSoy bean Brinjal Tomato Corn Potato Canola

Soil Micro flora

Selectable

MarkerOthers

2006-07 2 - - - - - - 40 6 7 28 17 19 - - -2005 14 5 6 6 7 4 5 25 4 7 15 13 12 2 3 32004 3 10 4 6 10 2 2 26 0 7 1 13 10 8 1 42003 5 7 10 10 13 6 1 20 1 8 1 9 8 8 5 82002 3 6 8 3 4 6 3 13 0 2 0 8 12 4 1 52001 4 5 8 8 5 5 4 12 0 3 1 9 7 3 0 92000 7 5 3 4 8 6 1 14 0 2 1 7 5 3 1 91999 2 1 1 1 3 0 2 7 0 3 0 3 5 1 1 41998 1 0 0 0 3 0 2 5 0 5 2 2 7 0 0 21997 0 0 1 1 1 1 2 0 0 2 1 0 1 0 0 1Total 41 39 41 39 54 30 22 162 11 46 50 81 86 29 12 45

Total number of records = 788

Current status of information stored in GM-Publication Database- June 2007

1. Indian Agricultural Research Institute 2. National Research Centre on Plant Biotechnology

3. Mahatma Phule Krishi Vidyapeeth4. National Bureau of Plant Genetic Resources5. Tata Energy Research Institute, TERI6. NRC for Sorghum7. Jawaharlal Nehru Krishi Vishwa Vidyalaya8. Tamilnadu Agricultural University9. Central Food Technological Research Institute10. Israel Life Science Industry11. Vasantdada Sugar Institute, Pune12. Centre for DNA Fingerprinting and Diagnostics13. Kerala Agricultural University14. Cornell University15. Central Rice Research Institute16. Dr. Babasaheb Ambedkar Marathwada University

17. Dr.Balasaheb Sawant Konkan Krishi Vidyapeeth18. Rajasthan Agricultural University, Bikaner19. Dhaka University20. Reuters India21. Central Institute for Cotton Research

22. Sathyabama University23.Osmania University24. Central Research Institute For Jute & Allied

Fibres25. Bishop Heber College, Trichi, TAMILNADU26. Amity Institute of Biotechnology27. Birla Institute of Technology28. Ministry of Environment and Forests -29. University of Agricultural Sciences,

BANGALORE30. Gauhati University31. University of Agricultural Sciences Dharwad32. Kerala university33. Directorate of Rice Research34. Junagadh Agricultural University35. Punjab State Council for Science & Technology36. Sher-e-Kashmir University of Agricultural

Sciences37. IARI Regional Station, Pusa, Bihar38. Central Soil Salinity Research Institute39. National Research Centre for Rapeseed

Mustard40. Central Institute of Freshwater Aquaculture41. Apeejay institute of Management42. Delhi University

List of Important Users of DB

Total Number of Hits in the Database = 2450

1. Crop Design2. Monsanto3. Tranzpac4. Emerging Solutions

5. JK-Agrigenetics Ltd6. GrasslanD Technology7. MAHYCO

Feedback Query Form

User`s Feedback

National Training Workshopson

Biosafety Issues and Web Resources in GMOs

Dr. R. K. KatiyarCourse Director &DirectorNational Research Centre on Plant Biotechnology, IARI, New Delhi –110012Email:rkkatiyar@hotmail.com

Dr. K. R. KoundalCourse Co-Director &Joint Director ResearchIndian Agricultural Research Institute, New Delhi -110012Email:jd_research@iari.res.in

Dr. T. R. SharmaCourse CoordinatorNational Research Centre on Plant Biotechnology, IARI, New Delhi –110012

Objective 4

First Training : April 23-25, 2007Second Training : May 14-16, 2007Third Training : June 7-9, 2007

Numbers and Period of Trainings

Participants

Total No. of Participants = 45ICAR Institutes Represented = 18SAUs represented = 13

Resource personsICARDBTDUCSIRSAUsICRISATMOEFBCIL

About the Participants and Resource Persons

Major Areas Covered in the training

•Biosafety issues Related to GMOs •GM crops development and their post release management •GMO detection in food feed and seeds•Cartagena protocol & safe trans boundary movements of GMOs•Environmental and health concerns related to GMOs •Regulatory issues and regulatory agencies•Availability and use of Web Resources on GMOs etc.•Practical on Web based Resource Utilization

Training Manuals Developed

A Technical Bulletin

Some Events of the 1st Training Workshop

Some Events of the 2nd Training Workshop

Some Events of the 3rd Training Workshop

Participants Feed Back about the Training

Outcome of the Project

A Database: A web based Comprehensive database on Biosafety Issues related to GMOs

Publications1. Sharma, T.R. K Srivastava, S.Singh and K.R. Koundal. 2006. Development of an Integrated Database on Biosafety and use of GMOS in India. Proc. Int Conf Implications of the Cartagena Protocol on Biosafety, Org by: Ministry Environment and Forests, Govt. of India. New Delhi .169-186.2. Koundal K.R. and T.R. Sharma. 2006. Biosafety concerns associated with GMOs. Proc. Int Conf Implications of the Cartagena Protocol on Biosafety, Org by: Ministry Environment and Forests, Govt. of India. New Delhi .83-893. Sharma,T.R., K. Srivastava, S. Singh, A. Kumar, K. Gaikwad, K.C. Bansal, N.K. Singh and K.R. Koundal.2007. Web based Information Resources on GMOs. In CRTAGENA Potocols on Biosafety:Decisions to Diagnostics.(Eds Ramdhawa et l.2007)NBPGR,New Delhi 179p.

Technical Bulletin

Sharma TR . 2007. GM Crops Database: An Interactive Web Resource. Pp. 16.

Training Manuals1.Sharma TR, KR Koundal, RK Katiyar 2007. 1st National Training Workshop on Biosafety Issue and Web Resources. April 23-25.pp682.Sharma TR, KR Koundal, RK Katiyar 2007. 2nd National Training Workshop on Biosafety Issue and Web Resources. May 14-16.pp76.3.Sharma TR, KR Koundal, RK Katiyar 2007. 3rd National Training Workshop on Biosafety Issue and Web Resources. June 7-9. pp80

Infrastructure Developed under the Project

•SUN FIRE V240 SERVER SYSTEM : One number•Storage Data Workstations : Two numbers.

Name of the personnel Period Present position

Research Associates

Ms Keerti Srivastava

Mr. Ajay Kumar Mehato

28-3-2005 to 30-9-06

1-10-2006 to 30-6-2007

Joined in IT Company

Tomato Genome Seq

ProjectSenior Research Fellows

Ms Sangeeta Singh

Ms.Charu Singh

Mr. N. Reddy

15-4-2005 to 30-6-2007

2-3-2007 to 30-62007

20-3.2007 to 8-6-2007

Pigeonpea Genome Project

ICAR Network Project on

Transgenic Crops

Joined in Ph D

Developed Trained Human Resource in Bioinformatics

Proposed work for the 2nd Phase

Updating the Database.

To develop a Network of Centres for Making Database in Regional Languages (Hindi, Punjabi, Tamil, Telgu and Kanada).

Developing a simple version of the database for the farming community and common public.

Acknowledgements

Financial AssistanceMinistry of Env. & Forest, GOIGEF- World Bank

Prof. K.R. KoundalJoint Director Research, IARI

Dr. PA KumarProject Director, NRCPB

KeertiAjaySangeetaCharu