Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud,...
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Transcript of Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud,...
![Page 1: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/1.jpg)
Comparative Metaganomics of the Deepwater Horizon Disaster
Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson
![Page 2: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/2.jpg)
Background
• Spill– April 20, 2010
• Collection– May 31, 2010– Depth = ???– Temperature = ???– Etc. = ???
• Capped, declared dead – Sept 19, 2010
![Page 3: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/3.jpg)
Initial Research Questions
• How is the Deepwater Horizon biome different from a pristine environment biome– Station Aloha
• How is the Deepwater Horizon biome different from an oxygen minimization zone?– Bioremediation of an oil spill (anthropogenic &
natural) consumes oxygen
![Page 4: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/4.jpg)
Gamma Proteobacteria are known to Degrade a wide range of organic matter
Cyanobacteria are expectedin the upper euphotic zone
Upper Euphotic (Aloha) OMZ DWH
![Page 5: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/5.jpg)
Upper eutrophic(Station Aloha)
OMZ Deepwater Horizon
![Page 6: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/6.jpg)
What we found
• Deepwater Horizon biome isn’t much like Station Aloha
• It also isn’t much like an OMZ
![Page 7: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/7.jpg)
What we found
• Deepwater Horizon biome isn’t much like Station Aloha
• It also isn’t much like an OMZ• Need better questions• If an oil spill isn’t like an OMZ, what is it like?
Whale fall???
![Page 8: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/8.jpg)
New Research Question
• How does the Deepwater Horizon environment differ from a whale fall?
![Page 9: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/9.jpg)
Whale & DWH both show high proteobacteria abundance
![Page 10: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/10.jpg)
![Page 11: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/11.jpg)
Gammaproteos, and Alcanivorax in particular, are known hydrocarbon degraders
Figure taken from Yakimov et al. Obligate oil-degrading marine bacteria. Current Opinion in Microbiology. 2007.
![Page 12: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/12.jpg)
Phylogeny
Alcanivoraxborkumensis (WF)
Colwelliapyschrerythraea (DWH)
![Page 13: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/13.jpg)
Metabolism of xenobiotics by cytochrome P450
Alkanes are only C source in Alcanovorax
![Page 14: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/14.jpg)
Figure from Schneikeret al.Nature Biotechnology. 2006. doi:10.1038/nbt1232
AlcanivoraxCytochrome P450 is involved in xenobiotic metabolism
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Pathways responsible for alkane degradation in Alcanovorax&Colwellia genomes
![Page 16: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/16.jpg)
Summary• DWH is not very similar to OMZ or oligotrophic open
ocean• DWH shares common features with whale fall– Abundance of proteobacteria, especially those with
potential for degrading hydrocarbons• IMG tools provided sufficient evidence to formulate
hypotheses that could be tested in subsequent lab experiments
• Praise: Easy to toggle between metagenomes and individual genomes of abundant organisms
• Wish: More descriptive metadata on DWH study
![Page 17: Comparative Metaganomics of the Deepwater Horizon Disaster Phil Heller, SlavaIvanenko, Huda Mahmoud, Zhen Quian, Judson.](https://reader036.fdocuments.in/reader036/viewer/2022062421/56649ced5503460f949ba988/html5/thumbnails/17.jpg)
Acknowledgements
• British Petroleum
• The Whale