COMATOGAP -...
Transcript of COMATOGAP -...
CHROMATOGRAPHY
Think about foodCare about safety
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot DeutschlandFrankreichMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-Mail sales-frmn-netcom
SchweizMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-Mail sales-chmn-netcom
Deutschlandund InternationalTel +49 24 21 969-0Fax +49 24 21 969-199E-Mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008ZERTIFIZIERT
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-Mail sales-usmn-netcom
Vol 3Antibiotics
infomn-netcom wwwmn-netcom2 3
Focus on antibiotics The global production and trading of food and feed products makes monitoring and control difficult
Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life
MN shows examples for food analysis that will help you with your work and make our food safer
Vol 3 Antibiotics in food of animal origin
Healthy animals provide healthy food
Antibiotics or antimicrobials are primarily used to treat infections caused by bacteria and other microorganisms but also they show growth promoting effects
In order to have the possibility to prevent diseases a ju-dicious use of antimicrobials is necessary since bacteria can be changed and then antibiotics are no longer effec-tive in people who need them to treat infections Antibiot-ic resistance is now a major threat to public health[WHO ndash World Health Organisation ndash httpwwwwhointmediacentrenewsreleases2014amr-reporten]
There are different classes of antimicrobials egbullPenicillinsbullAminoglycosidesbullArsenicalsbullPolypeptidesbullGlycolipidsbullTetracyclinesbullMacrolidesbullLincosamidesbullβ-LactamsbullQuinolones fluoroquinolonebullStreptograminsbullSulfonamidesbulland others
[Wikipedia ndash httpenwikipediaorg wikiList_of_antibiotics]
Most of these examples are antibiotics that were first iso-lated from a natural source Nowadays they are mostly produced synthetically and new antibiotics are usually semisynthetic modifications
Most of the antibiotics used in food animals are the
same as those used in humans
Due to the importance of antibiotic resistance as a public health problem
many governments and professional societies have reviewed existing scien-tific evidence and developed recommen-dations to limit all types of antibiotic use including use in food animals [National Anti-
microbial Resistance Monitoring System (NARMS) ndash FDA httpwwwfdagovAnimalVeterinarySafety
HealthAntimicrobialResistanceNationalAntimi-crobialResistanceMonitoringSystemdefaulthtm
ndash CDC httpwwwcdcgovnarms ndash USDA httpwwwarsusdagovsaabearnarms]
infomn-netcom wwwmn-netcom2 3
Compounds of interestChemical structures of important antibiotics
QuinolonesCiprofloxacin (R = H)
Enrofloxacin (R = C2H5)COOH
F
NO
N
NR
Norfloxacin (R = H)
Pefloxacin (R = CH3)
H5C2
NO
NCOOH
F
N
RH
Miloxacin (R = OCH3)
Oxolinic acid (R = C2H5) COOH
R
O
N
OO
Nalidixic acid
COOH
H5C2
H3CO
NN
Sarafloxacin
NH ndash NCOOH
F
F
O
N
Flumequine
COOH
CH3
FO
N
Macrolides Erythromycin Spiramycin
O
CH3
CH3OCH3
O HO
H3C
ON(CH3)2
O HO
H3C
H3CH3CH5C2 O
O
CH3
CH3
CH3OHOHHO
O
OO
O
O
O
O O
OH
N
N
H3C
H3C
H3C
H3CO
CH3
O OH
OH
CH3
H3C
HOCH3
CH3
CH3
CH3
O
β-Lactams Ampicillin Amoxycillin Penicillin G
O
OO
N
S
NH NH2
H3CH3C
HOO
HO
OO
N
S
NH NH2
OH
H3CH3C
O
OO
N
S
NH
H3CH3C
HO
Miscellaneous Chloramphenicol Thiamphenicol Florfenicol
O2N HO O
HN
OH Cl
ClS HO O
HN
OH
H3C
Cl
Cl
O OS F O
HN
OH
H3C
Cl
Cl
O O
Trimethoprim Furazolidone Tiamulin
NO
O
ON
CH3
CH3
CH3
NH2
H2NO
ONN
O2N O
O
O
OH
HSN
O
H3C
H3C H3CH3C
CH3
CH3
H2C
Chemical structures of sulfonamides and tetracyclines see page 5
infomn-netcom wwwmn-netcom4 5
Typical samples preparationSPE of veterinary drugs from fish
MN AAp MNo 305080JM Stevens et al GIT Spezial Separation 12009 16ndash17Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg SA 3 mL 500 mg REF 730077
Column conditioning 2 x 3 mL n-hexane drying by vacuum then 3 mL 05 acetic acid in ethyl acetate do not let run the column drySample aspiration 5ndash8 mLmin then 10ndash12 min dryingColumn washing 10 mL methanolElution 5 mL 10 triethanolamine in methanolCompounds investigated sulfamerazine sulfadimidine sulfadimethoxine trimethoprim sulfadiazine chlortetracycline oxytetracycline ampicillin amoxycillin benzylpenicillin ciprofloxacin enrofloxacin norfloxacin oxolinic acid pefloxacin flumequine sarafloxacin furazolidone erythromycin spiramycin gentamicin chloram-phenicol florfenicol thiamphenicol tiamulin nalidixic acid miloxacinRecovery rates 85 to100 for spiked samples with concen-trations from 0001 to 0100 mgmL
SamplefiltrationandstorageIt is recommended to filter the prepared sample through dis-posable filters CHROMAFILregXtraPVDF-4525 REF 729219
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871
116
x 3
2 m
mSPE of tetracyclines from musculature
MN AAp MNo 302030Courtesy of Mr Lippold Chemisches Landesuntersuchungs-amt (Chem Research Agency) Freiburg GermanySample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg Tetracycline 6 mL 500 mg REF 730315
Column conditioning 1 column volume methanol 1 column volume dist water then 1 column volume EDTA-succinate buffer (see description at wwwmn-netcomaAAs) do not let run the column drySample application Slowly force or aspirate the eluate of the sample pretreatment through the columnColumn washing 2 mL dist water (removal of Cu ions) 1 mL n-hexaneElution Elute with 75 mL methanol in a 25 mL tapered flask Add 1 mL of an ethylene glycol ndash methanol mixture (22 g ethylene glycol are filled up with methanol to 100 mL) and evaporate with a rotation evaporator (max 40 degC) to dryness Fill up the residue with 01 molL McIlvain-EDTA buffer (dissolve 525 g citric acid x H2O 445 g Na2HPO4 x H2O and 93 g Titriplex III in 25 L dist water adjust with NaOH to pH 4) to 400 mLCompounds investigated tetracycline oxytetracycline chlortetracycline 100ndash500 mgkgRecovery ratestetracycline chlortetracycline ca 50ndash70 oxytetracycline ca 60ndash80
CHROMABONDreg vacuum manifold for 12 columns REF 730150
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
AnalysisSubsequentanalysisHPLCSulfonamides
MN AAp MNo 125860 Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm
REF 76057830
0 2 4 6 8 min
1
23
4
5
6
7
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfapyridine4 Sulfamerazine5 Sulfadimidine6 Sulfathiazole7 Sulfadimethoxine
Sulfapyridine
HNN NH2
O
OS
Sulfathiazole
SHN
O
O
SN
NH2
Sulfachloropyridazine Sulfaquinoxaline
NN HN NH2
O
OS
ClHNN
N
NH2
O
OS
Sulfadiazine R1=R2=HSulfamerazine R1 = H R2 = CH3Sulfadimidine R1 = R2 = CH3 Sulfadimethoxine
NN
HN NH2
O
OS
R2
R1 NN
HN NH2
O
OS
OCH3
H3CO
TetracyclinesMN AAp MNo 127050
Column EC 502 NUCLEOSHELLreg RP 18plus 27 μm REF 76323220
0 1 2 3 4 min
1
2
3
4
5
OH O O OHO
OH
NH2
N(CH3)2R4 R1R3
OH
R2
Peaks R1 R2 R3 R4
1 Minocycline H H H NH22 Oxytetracycline OH OH CH3 H3 Tetracycline H OH CH3 H4 Chlortetracycline H OH CH3 Cl5 Doxycycline OH H CH3 H
Detailed conditions are available online at wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom6 7
Analysis Antibacterial agents
MN AAp MNo 124190Column EC 15046 NUCLEODURreg C18 HTec 3 μm
REF 76032546
8
7
65
4
3
2
1
9
10
11
0 1 2 3 4 5 6 7 8 min
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfathiazole4 Sulfamerazine5 Sulfapyridine6 Sulfaquinoxaline7 Sulfadimethoxine8 Sulfadimidine9 Furazolidone10 Nalidixic acid11 Oxolinic acid
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077
Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg
30 730315
Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom
SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom
Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
local distributor
KATE
N20
0119
Foc
us F
lyer
Foo
d Vo
l 3
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Bob
o s
hock
ndash fo
tolia
com
infomn-netcom wwwmn-netcom2 3
Focus on antibiotics The global production and trading of food and feed products makes monitoring and control difficult
Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life
MN shows examples for food analysis that will help you with your work and make our food safer
Vol 3 Antibiotics in food of animal origin
Healthy animals provide healthy food
Antibiotics or antimicrobials are primarily used to treat infections caused by bacteria and other microorganisms but also they show growth promoting effects
In order to have the possibility to prevent diseases a ju-dicious use of antimicrobials is necessary since bacteria can be changed and then antibiotics are no longer effec-tive in people who need them to treat infections Antibiot-ic resistance is now a major threat to public health[WHO ndash World Health Organisation ndash httpwwwwhointmediacentrenewsreleases2014amr-reporten]
There are different classes of antimicrobials egbullPenicillinsbullAminoglycosidesbullArsenicalsbullPolypeptidesbullGlycolipidsbullTetracyclinesbullMacrolidesbullLincosamidesbullβ-LactamsbullQuinolones fluoroquinolonebullStreptograminsbullSulfonamidesbulland others
[Wikipedia ndash httpenwikipediaorg wikiList_of_antibiotics]
Most of these examples are antibiotics that were first iso-lated from a natural source Nowadays they are mostly produced synthetically and new antibiotics are usually semisynthetic modifications
Most of the antibiotics used in food animals are the
same as those used in humans
Due to the importance of antibiotic resistance as a public health problem
many governments and professional societies have reviewed existing scien-tific evidence and developed recommen-dations to limit all types of antibiotic use including use in food animals [National Anti-
microbial Resistance Monitoring System (NARMS) ndash FDA httpwwwfdagovAnimalVeterinarySafety
HealthAntimicrobialResistanceNationalAntimi-crobialResistanceMonitoringSystemdefaulthtm
ndash CDC httpwwwcdcgovnarms ndash USDA httpwwwarsusdagovsaabearnarms]
infomn-netcom wwwmn-netcom2 3
Compounds of interestChemical structures of important antibiotics
QuinolonesCiprofloxacin (R = H)
Enrofloxacin (R = C2H5)COOH
F
NO
N
NR
Norfloxacin (R = H)
Pefloxacin (R = CH3)
H5C2
NO
NCOOH
F
N
RH
Miloxacin (R = OCH3)
Oxolinic acid (R = C2H5) COOH
R
O
N
OO
Nalidixic acid
COOH
H5C2
H3CO
NN
Sarafloxacin
NH ndash NCOOH
F
F
O
N
Flumequine
COOH
CH3
FO
N
Macrolides Erythromycin Spiramycin
O
CH3
CH3OCH3
O HO
H3C
ON(CH3)2
O HO
H3C
H3CH3CH5C2 O
O
CH3
CH3
CH3OHOHHO
O
OO
O
O
O
O O
OH
N
N
H3C
H3C
H3C
H3CO
CH3
O OH
OH
CH3
H3C
HOCH3
CH3
CH3
CH3
O
β-Lactams Ampicillin Amoxycillin Penicillin G
O
OO
N
S
NH NH2
H3CH3C
HOO
HO
OO
N
S
NH NH2
OH
H3CH3C
O
OO
N
S
NH
H3CH3C
HO
Miscellaneous Chloramphenicol Thiamphenicol Florfenicol
O2N HO O
HN
OH Cl
ClS HO O
HN
OH
H3C
Cl
Cl
O OS F O
HN
OH
H3C
Cl
Cl
O O
Trimethoprim Furazolidone Tiamulin
NO
O
ON
CH3
CH3
CH3
NH2
H2NO
ONN
O2N O
O
O
OH
HSN
O
H3C
H3C H3CH3C
CH3
CH3
H2C
Chemical structures of sulfonamides and tetracyclines see page 5
infomn-netcom wwwmn-netcom4 5
Typical samples preparationSPE of veterinary drugs from fish
MN AAp MNo 305080JM Stevens et al GIT Spezial Separation 12009 16ndash17Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg SA 3 mL 500 mg REF 730077
Column conditioning 2 x 3 mL n-hexane drying by vacuum then 3 mL 05 acetic acid in ethyl acetate do not let run the column drySample aspiration 5ndash8 mLmin then 10ndash12 min dryingColumn washing 10 mL methanolElution 5 mL 10 triethanolamine in methanolCompounds investigated sulfamerazine sulfadimidine sulfadimethoxine trimethoprim sulfadiazine chlortetracycline oxytetracycline ampicillin amoxycillin benzylpenicillin ciprofloxacin enrofloxacin norfloxacin oxolinic acid pefloxacin flumequine sarafloxacin furazolidone erythromycin spiramycin gentamicin chloram-phenicol florfenicol thiamphenicol tiamulin nalidixic acid miloxacinRecovery rates 85 to100 for spiked samples with concen-trations from 0001 to 0100 mgmL
SamplefiltrationandstorageIt is recommended to filter the prepared sample through dis-posable filters CHROMAFILregXtraPVDF-4525 REF 729219
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871
116
x 3
2 m
mSPE of tetracyclines from musculature
MN AAp MNo 302030Courtesy of Mr Lippold Chemisches Landesuntersuchungs-amt (Chem Research Agency) Freiburg GermanySample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg Tetracycline 6 mL 500 mg REF 730315
Column conditioning 1 column volume methanol 1 column volume dist water then 1 column volume EDTA-succinate buffer (see description at wwwmn-netcomaAAs) do not let run the column drySample application Slowly force or aspirate the eluate of the sample pretreatment through the columnColumn washing 2 mL dist water (removal of Cu ions) 1 mL n-hexaneElution Elute with 75 mL methanol in a 25 mL tapered flask Add 1 mL of an ethylene glycol ndash methanol mixture (22 g ethylene glycol are filled up with methanol to 100 mL) and evaporate with a rotation evaporator (max 40 degC) to dryness Fill up the residue with 01 molL McIlvain-EDTA buffer (dissolve 525 g citric acid x H2O 445 g Na2HPO4 x H2O and 93 g Titriplex III in 25 L dist water adjust with NaOH to pH 4) to 400 mLCompounds investigated tetracycline oxytetracycline chlortetracycline 100ndash500 mgkgRecovery ratestetracycline chlortetracycline ca 50ndash70 oxytetracycline ca 60ndash80
CHROMABONDreg vacuum manifold for 12 columns REF 730150
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
AnalysisSubsequentanalysisHPLCSulfonamides
MN AAp MNo 125860 Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm
REF 76057830
0 2 4 6 8 min
1
23
4
5
6
7
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfapyridine4 Sulfamerazine5 Sulfadimidine6 Sulfathiazole7 Sulfadimethoxine
Sulfapyridine
HNN NH2
O
OS
Sulfathiazole
SHN
O
O
SN
NH2
Sulfachloropyridazine Sulfaquinoxaline
NN HN NH2
O
OS
ClHNN
N
NH2
O
OS
Sulfadiazine R1=R2=HSulfamerazine R1 = H R2 = CH3Sulfadimidine R1 = R2 = CH3 Sulfadimethoxine
NN
HN NH2
O
OS
R2
R1 NN
HN NH2
O
OS
OCH3
H3CO
TetracyclinesMN AAp MNo 127050
Column EC 502 NUCLEOSHELLreg RP 18plus 27 μm REF 76323220
0 1 2 3 4 min
1
2
3
4
5
OH O O OHO
OH
NH2
N(CH3)2R4 R1R3
OH
R2
Peaks R1 R2 R3 R4
1 Minocycline H H H NH22 Oxytetracycline OH OH CH3 H3 Tetracycline H OH CH3 H4 Chlortetracycline H OH CH3 Cl5 Doxycycline OH H CH3 H
Detailed conditions are available online at wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom6 7
Analysis Antibacterial agents
MN AAp MNo 124190Column EC 15046 NUCLEODURreg C18 HTec 3 μm
REF 76032546
8
7
65
4
3
2
1
9
10
11
0 1 2 3 4 5 6 7 8 min
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfathiazole4 Sulfamerazine5 Sulfapyridine6 Sulfaquinoxaline7 Sulfadimethoxine8 Sulfadimidine9 Furazolidone10 Nalidixic acid11 Oxolinic acid
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077
Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg
30 730315
Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom
SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom
Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
local distributor
KATE
N20
0119
Foc
us F
lyer
Foo
d Vo
l 3
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Bob
o s
hock
ndash fo
tolia
com
infomn-netcom wwwmn-netcom2 3
Compounds of interestChemical structures of important antibiotics
QuinolonesCiprofloxacin (R = H)
Enrofloxacin (R = C2H5)COOH
F
NO
N
NR
Norfloxacin (R = H)
Pefloxacin (R = CH3)
H5C2
NO
NCOOH
F
N
RH
Miloxacin (R = OCH3)
Oxolinic acid (R = C2H5) COOH
R
O
N
OO
Nalidixic acid
COOH
H5C2
H3CO
NN
Sarafloxacin
NH ndash NCOOH
F
F
O
N
Flumequine
COOH
CH3
FO
N
Macrolides Erythromycin Spiramycin
O
CH3
CH3OCH3
O HO
H3C
ON(CH3)2
O HO
H3C
H3CH3CH5C2 O
O
CH3
CH3
CH3OHOHHO
O
OO
O
O
O
O O
OH
N
N
H3C
H3C
H3C
H3CO
CH3
O OH
OH
CH3
H3C
HOCH3
CH3
CH3
CH3
O
β-Lactams Ampicillin Amoxycillin Penicillin G
O
OO
N
S
NH NH2
H3CH3C
HOO
HO
OO
N
S
NH NH2
OH
H3CH3C
O
OO
N
S
NH
H3CH3C
HO
Miscellaneous Chloramphenicol Thiamphenicol Florfenicol
O2N HO O
HN
OH Cl
ClS HO O
HN
OH
H3C
Cl
Cl
O OS F O
HN
OH
H3C
Cl
Cl
O O
Trimethoprim Furazolidone Tiamulin
NO
O
ON
CH3
CH3
CH3
NH2
H2NO
ONN
O2N O
O
O
OH
HSN
O
H3C
H3C H3CH3C
CH3
CH3
H2C
Chemical structures of sulfonamides and tetracyclines see page 5
infomn-netcom wwwmn-netcom4 5
Typical samples preparationSPE of veterinary drugs from fish
MN AAp MNo 305080JM Stevens et al GIT Spezial Separation 12009 16ndash17Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg SA 3 mL 500 mg REF 730077
Column conditioning 2 x 3 mL n-hexane drying by vacuum then 3 mL 05 acetic acid in ethyl acetate do not let run the column drySample aspiration 5ndash8 mLmin then 10ndash12 min dryingColumn washing 10 mL methanolElution 5 mL 10 triethanolamine in methanolCompounds investigated sulfamerazine sulfadimidine sulfadimethoxine trimethoprim sulfadiazine chlortetracycline oxytetracycline ampicillin amoxycillin benzylpenicillin ciprofloxacin enrofloxacin norfloxacin oxolinic acid pefloxacin flumequine sarafloxacin furazolidone erythromycin spiramycin gentamicin chloram-phenicol florfenicol thiamphenicol tiamulin nalidixic acid miloxacinRecovery rates 85 to100 for spiked samples with concen-trations from 0001 to 0100 mgmL
SamplefiltrationandstorageIt is recommended to filter the prepared sample through dis-posable filters CHROMAFILregXtraPVDF-4525 REF 729219
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871
116
x 3
2 m
mSPE of tetracyclines from musculature
MN AAp MNo 302030Courtesy of Mr Lippold Chemisches Landesuntersuchungs-amt (Chem Research Agency) Freiburg GermanySample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg Tetracycline 6 mL 500 mg REF 730315
Column conditioning 1 column volume methanol 1 column volume dist water then 1 column volume EDTA-succinate buffer (see description at wwwmn-netcomaAAs) do not let run the column drySample application Slowly force or aspirate the eluate of the sample pretreatment through the columnColumn washing 2 mL dist water (removal of Cu ions) 1 mL n-hexaneElution Elute with 75 mL methanol in a 25 mL tapered flask Add 1 mL of an ethylene glycol ndash methanol mixture (22 g ethylene glycol are filled up with methanol to 100 mL) and evaporate with a rotation evaporator (max 40 degC) to dryness Fill up the residue with 01 molL McIlvain-EDTA buffer (dissolve 525 g citric acid x H2O 445 g Na2HPO4 x H2O and 93 g Titriplex III in 25 L dist water adjust with NaOH to pH 4) to 400 mLCompounds investigated tetracycline oxytetracycline chlortetracycline 100ndash500 mgkgRecovery ratestetracycline chlortetracycline ca 50ndash70 oxytetracycline ca 60ndash80
CHROMABONDreg vacuum manifold for 12 columns REF 730150
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
AnalysisSubsequentanalysisHPLCSulfonamides
MN AAp MNo 125860 Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm
REF 76057830
0 2 4 6 8 min
1
23
4
5
6
7
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfapyridine4 Sulfamerazine5 Sulfadimidine6 Sulfathiazole7 Sulfadimethoxine
Sulfapyridine
HNN NH2
O
OS
Sulfathiazole
SHN
O
O
SN
NH2
Sulfachloropyridazine Sulfaquinoxaline
NN HN NH2
O
OS
ClHNN
N
NH2
O
OS
Sulfadiazine R1=R2=HSulfamerazine R1 = H R2 = CH3Sulfadimidine R1 = R2 = CH3 Sulfadimethoxine
NN
HN NH2
O
OS
R2
R1 NN
HN NH2
O
OS
OCH3
H3CO
TetracyclinesMN AAp MNo 127050
Column EC 502 NUCLEOSHELLreg RP 18plus 27 μm REF 76323220
0 1 2 3 4 min
1
2
3
4
5
OH O O OHO
OH
NH2
N(CH3)2R4 R1R3
OH
R2
Peaks R1 R2 R3 R4
1 Minocycline H H H NH22 Oxytetracycline OH OH CH3 H3 Tetracycline H OH CH3 H4 Chlortetracycline H OH CH3 Cl5 Doxycycline OH H CH3 H
Detailed conditions are available online at wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom6 7
Analysis Antibacterial agents
MN AAp MNo 124190Column EC 15046 NUCLEODURreg C18 HTec 3 μm
REF 76032546
8
7
65
4
3
2
1
9
10
11
0 1 2 3 4 5 6 7 8 min
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfathiazole4 Sulfamerazine5 Sulfapyridine6 Sulfaquinoxaline7 Sulfadimethoxine8 Sulfadimidine9 Furazolidone10 Nalidixic acid11 Oxolinic acid
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077
Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg
30 730315
Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom
SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom
Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
local distributor
KATE
N20
0119
Foc
us F
lyer
Foo
d Vo
l 3
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Bob
o s
hock
ndash fo
tolia
com
infomn-netcom wwwmn-netcom4 5
Typical samples preparationSPE of veterinary drugs from fish
MN AAp MNo 305080JM Stevens et al GIT Spezial Separation 12009 16ndash17Sample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg SA 3 mL 500 mg REF 730077
Column conditioning 2 x 3 mL n-hexane drying by vacuum then 3 mL 05 acetic acid in ethyl acetate do not let run the column drySample aspiration 5ndash8 mLmin then 10ndash12 min dryingColumn washing 10 mL methanolElution 5 mL 10 triethanolamine in methanolCompounds investigated sulfamerazine sulfadimidine sulfadimethoxine trimethoprim sulfadiazine chlortetracycline oxytetracycline ampicillin amoxycillin benzylpenicillin ciprofloxacin enrofloxacin norfloxacin oxolinic acid pefloxacin flumequine sarafloxacin furazolidone erythromycin spiramycin gentamicin chloram-phenicol florfenicol thiamphenicol tiamulin nalidixic acid miloxacinRecovery rates 85 to100 for spiked samples with concen-trations from 0001 to 0100 mgmL
SamplefiltrationandstorageIt is recommended to filter the prepared sample through dis-posable filters CHROMAFILregXtraPVDF-4525 REF 729219
Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871
116
x 3
2 m
mSPE of tetracyclines from musculature
MN AAp MNo 302030Courtesy of Mr Lippold Chemisches Landesuntersuchungs-amt (Chem Research Agency) Freiburg GermanySample pretreatment see wwwmn-netcomaAAs
c Column type CHROMABONDreg Tetracycline 6 mL 500 mg REF 730315
Column conditioning 1 column volume methanol 1 column volume dist water then 1 column volume EDTA-succinate buffer (see description at wwwmn-netcomaAAs) do not let run the column drySample application Slowly force or aspirate the eluate of the sample pretreatment through the columnColumn washing 2 mL dist water (removal of Cu ions) 1 mL n-hexaneElution Elute with 75 mL methanol in a 25 mL tapered flask Add 1 mL of an ethylene glycol ndash methanol mixture (22 g ethylene glycol are filled up with methanol to 100 mL) and evaporate with a rotation evaporator (max 40 degC) to dryness Fill up the residue with 01 molL McIlvain-EDTA buffer (dissolve 525 g citric acid x H2O 445 g Na2HPO4 x H2O and 93 g Titriplex III in 25 L dist water adjust with NaOH to pH 4) to 400 mLCompounds investigated tetracycline oxytetracycline chlortetracycline 100ndash500 mgkgRecovery ratestetracycline chlortetracycline ca 50ndash70 oxytetracycline ca 60ndash80
CHROMABONDreg vacuum manifold for 12 columns REF 730150
For other applications see wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom4 5
AnalysisSubsequentanalysisHPLCSulfonamides
MN AAp MNo 125860 Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm
REF 76057830
0 2 4 6 8 min
1
23
4
5
6
7
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfapyridine4 Sulfamerazine5 Sulfadimidine6 Sulfathiazole7 Sulfadimethoxine
Sulfapyridine
HNN NH2
O
OS
Sulfathiazole
SHN
O
O
SN
NH2
Sulfachloropyridazine Sulfaquinoxaline
NN HN NH2
O
OS
ClHNN
N
NH2
O
OS
Sulfadiazine R1=R2=HSulfamerazine R1 = H R2 = CH3Sulfadimidine R1 = R2 = CH3 Sulfadimethoxine
NN
HN NH2
O
OS
R2
R1 NN
HN NH2
O
OS
OCH3
H3CO
TetracyclinesMN AAp MNo 127050
Column EC 502 NUCLEOSHELLreg RP 18plus 27 μm REF 76323220
0 1 2 3 4 min
1
2
3
4
5
OH O O OHO
OH
NH2
N(CH3)2R4 R1R3
OH
R2
Peaks R1 R2 R3 R4
1 Minocycline H H H NH22 Oxytetracycline OH OH CH3 H3 Tetracycline H OH CH3 H4 Chlortetracycline H OH CH3 Cl5 Doxycycline OH H CH3 H
Detailed conditions are available online at wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom6 7
Analysis Antibacterial agents
MN AAp MNo 124190Column EC 15046 NUCLEODURreg C18 HTec 3 μm
REF 76032546
8
7
65
4
3
2
1
9
10
11
0 1 2 3 4 5 6 7 8 min
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfathiazole4 Sulfamerazine5 Sulfapyridine6 Sulfaquinoxaline7 Sulfadimethoxine8 Sulfadimidine9 Furazolidone10 Nalidixic acid11 Oxolinic acid
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077
Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg
30 730315
Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom
SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom
Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
local distributor
KATE
N20
0119
Foc
us F
lyer
Foo
d Vo
l 3
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Bob
o s
hock
ndash fo
tolia
com
infomn-netcom wwwmn-netcom4 5
AnalysisSubsequentanalysisHPLCSulfonamides
MN AAp MNo 125860 Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm
REF 76057830
0 2 4 6 8 min
1
23
4
5
6
7
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfapyridine4 Sulfamerazine5 Sulfadimidine6 Sulfathiazole7 Sulfadimethoxine
Sulfapyridine
HNN NH2
O
OS
Sulfathiazole
SHN
O
O
SN
NH2
Sulfachloropyridazine Sulfaquinoxaline
NN HN NH2
O
OS
ClHNN
N
NH2
O
OS
Sulfadiazine R1=R2=HSulfamerazine R1 = H R2 = CH3Sulfadimidine R1 = R2 = CH3 Sulfadimethoxine
NN
HN NH2
O
OS
R2
R1 NN
HN NH2
O
OS
OCH3
H3CO
TetracyclinesMN AAp MNo 127050
Column EC 502 NUCLEOSHELLreg RP 18plus 27 μm REF 76323220
0 1 2 3 4 min
1
2
3
4
5
OH O O OHO
OH
NH2
N(CH3)2R4 R1R3
OH
R2
Peaks R1 R2 R3 R4
1 Minocycline H H H NH22 Oxytetracycline OH OH CH3 H3 Tetracycline H OH CH3 H4 Chlortetracycline H OH CH3 Cl5 Doxycycline OH H CH3 H
Detailed conditions are available online at wwwmn-netcomaAAs
infomn-netcom wwwmn-netcom6 7
Analysis Antibacterial agents
MN AAp MNo 124190Column EC 15046 NUCLEODURreg C18 HTec 3 μm
REF 76032546
8
7
65
4
3
2
1
9
10
11
0 1 2 3 4 5 6 7 8 min
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfathiazole4 Sulfamerazine5 Sulfapyridine6 Sulfaquinoxaline7 Sulfadimethoxine8 Sulfadimidine9 Furazolidone10 Nalidixic acid11 Oxolinic acid
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077
Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg
30 730315
Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom
SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom
Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
local distributor
KATE
N20
0119
Foc
us F
lyer
Foo
d Vo
l 3
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Bob
o s
hock
ndash fo
tolia
com
infomn-netcom wwwmn-netcom6 7
Analysis Antibacterial agents
MN AAp MNo 124190Column EC 15046 NUCLEODURreg C18 HTec 3 μm
REF 76032546
8
7
65
4
3
2
1
9
10
11
0 1 2 3 4 5 6 7 8 min
Peaks1 Sulfadiazine2 Sulfachloropyridazine3 Sulfathiazole4 Sulfamerazine5 Sulfapyridine6 Sulfaquinoxaline7 Sulfadimethoxine8 Sulfadimidine9 Furazolidone10 Nalidixic acid11 Oxolinic acid
Detailed conditions are available online at wwwmn-netcomaAAs
MN suggests using guard columns to protect your main column and significantly increase column life
The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116 fittings
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077
Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg
30 730315
Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom
SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom
Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
local distributor
KATE
N20
0119
Foc
us F
lyer
Foo
d Vo
l 3
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Bob
o s
hock
ndash fo
tolia
com
infomn-netcom wwwmn-netcom6 7
Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg SA 3 mL 500 mg 50 730077
Especially recommended for tetracyclines CHROMABONDreg Tetracycline 6 mL 500 mg
30 730315
Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284
Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red
100 7022871
HPLC1Column EC 1503 NUCLEODURreg Phenyl-Hexyl 3 μm 1 76057830Guard column EC 43 NUCLEODURreg Phenyl-Hexyl 3 μm 3 76198630Guard column holder Column Protection System 1 718966HPLC2Column EC 503 NUCLEOSHELLreg RP 18plus 27 μm 1 76323230Guard column EC 43 NUCLEOSHELLreg RP 18plus 27 μm 3 76323830Guard column holder Column Protection System 1 718966HPLC3Column EC 15046 NUCLEODURreg C18 HTec 3 μm 1 76032546Guard column EC 43 NUCLEODURreg C18 HTec 3 μm 3 76192630Guard column holder Column Protection System 1 718966
Visit us at wwwmn-netcomchroma to get more helpful information
Selection tools for
Vials and caps
Syringe filters
Troubleshooting guidesGC and HPLC
Detailed product information
hellipand much more
wwwmn-netcom
wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom
SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom
Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom
MACHEREY-NAGEL
EN ISO 9001 2008CERTIFIED
USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom
Food safety made easy with products from
MACHEREY-NAGEL
Vol 8
Additives
Vol 6
Mycotoxins
Vol 2
Vitamins
Vol 4
Acrylamide
Vol 1
Melamine
Vol 5
Sugars Sweeteners
Vol 3
AntibioticsVol 7
Pesticides
local distributor
KATE
N20
0119
Foc
us F
lyer
Foo
d Vo
l 3
en1
10
031
5 PD
Pr
inte
d in
Ger
man
yIm
age
cred
its copy
Tija
na o
lga
dem
schi
shin
a P
ierre
bril
lot
Pete
r Her
mes
Fur
ian
ar
enaf
otou
k fo
vito
nic
eday
rr1
45 a
irbor
ne77
Bob
o s
hock
ndash fo
tolia
com
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