Collision induced dissociative chemical cross-linking reagent for protein structure characterization
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Transcript of Collision induced dissociative chemical cross-linking reagent for protein structure characterization
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Collision-induced Dissociative Chemical Cross-linking Reagent for Protein Structure Characterization
Yiming ChenNov. 28, 2012
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J. Mass. Spectrom 2010, 45, 178-189
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Outline
• Introduction
• Chemistry of cross-linker
• Examples
• Conclusion
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Outline
• Introduction
• Abstract
• Edman Degradation
• Instrumentation
• Chemistry of cross-linker
• Examples
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Abstract
• Design an innovative cross-linker based on Edman degradation
• Form indicative mass shifted fragment ions and constant neutral losses
• Unambiguous identification of cross-linked peptide
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Edman Degradation
N C S H2NO
HN Peptide
R
H+
HN
S
NH O
HN Peptide
R
H+
N NH
SNHO
R
HPeptide
N NH
SO
R
N NHO
S
R
heat
Peptide-NH2
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Edman Degradation
• Phenylisothiocyanate is reacted with an uncharged terminal amino group
• Terminal amino acid is cleaved as a thiazolinone derivative
• Thiazolinone is treated with acid to form the more stable phenylthiohydantoin (PTH) that can be chracterized
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Edman Degradation
• Sequence peptides with 60 or less amino acid residues
• Sensitivity: 10-100 pico-moles
• Has been automated
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Instrumentation
• Linear MALDI-TOF
• nano-HPLC-MALDI-TOF/TOF-MS (offline)
• nano-HPLC/nano-ESI-LTQ-Orbitrap-MS
• Offline nano-ESI-LTQ-Orbitrap-MS
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Outline
• Introduction
• Chemistry of cross-linker
• Synthesis
• Mechanism of Reaction
• Example
• Conclusion
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SynthesisO
OHH2NSOCl2, MeOH O
OH2N
O
ONCS
CS2/CH2Cl2
HN
O
OHO
O
OO NOH
DCC
HN
O
OO
O N
O
O
+NH
O
OH
HN
O
O
O N
O
OH + H2NO
O
DCCHN
O
O
O N
O
NH O
O
Pd/H2O
N
O
NH O
OH2N +O
ONCSO
O HN
S
HN
O
N
O
NH O
O
LiOH/THF/H2OHO
O HN
S
HN
O
N
O
NH O
OHN O
OO
O
HN
S
HN
O
N
O
NH O
O N
O
O
NHS-BuTuGPG-NHS
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Cross-Linker
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Mechanism (1)
Inter-peptide cross-link
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Mechanism (2)
Intra-peptide cross-link
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Mechanism (3)
dead-end link
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Example: substance P
• Sequence: Arg Pro Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 (RPKPQQFFGLM-NH2)
• Associated with inflammatory processes and pain
• Molar Mass: 1347.63 g/mol
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Example: substance P
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Example: substance P
• Neutral loss of 184 m/z (doubly charged 843 m/z to 751 m/z) indicates cross-linking
• Two intrapeptidal cross-linking isomers can be distinguished by y10 fragment
• Dead-end link has characteristic neutral losses: 172 m/z or 202 m/z
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Example: LHRH
• Sequence: pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
• No intrapeptidal cross-link, only dead-end link
• Characteristic neutral losses: 172 m/z or 202 m/z
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Example: lysozyme
• Lysozyme is part of the innate immune system, 14.3 kDa
• Up to 6 cross-linkers attached
• Digested by trypsin and resulting peptides are analyzed by nano-HPLC/MALDI-TOF/TOF-MS and nano-HPLC/nano-ESI-LTQ-Orbitrap-MS
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Example: lysozyme
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Example: lysozyme
Residue 6-14
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Example: lysozyme
Residue 115-125
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Example: lysozyme
• For residue peptide 115-125, m/z 836 is correspondents to intrapeptidal cross-linking
• Results suggests Thr-118 is also involved beside Lys-116
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Outline
• Introduction
• Chemistry of cross-linker
• Examples
• Conclusion
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Conclusion
• Easy to synthesize
• Clear characterization
• Investigate tertiary or quaternery structures
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Thanks!