8/10/2019 Clinical Study: Validation of Non-Invasive Raman Spectroscopy as an Indicator of Total Antioxidant Nutritional Status in Humans

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Clinical Study: Validation of Non-Invasive Raman Spectroscopy as an Indicator of

Total Antioxidant Nutritional Status in Humans

Octoer !""#

Measurement of blood antioxidants is widely accepted as the gold standard method to determine

antioxidant nutritional status in human research studies. This method typically involves invasive bloodsampling, extraction and analysis of carotenoids, vitamin E and/or vitamin C by high performance liquid

chromatography !"#C$. %lood antioxidant analysis is not practical for use outside the clinic since it is

invasive, time&consuming, expensive and the blood levels are affected by multiple factors, such as

antioxidant consumption from recent meals.

'ecent research strongly supports the concept of an (antioxidant networ)* in the body in which different

antioxidant species wor) synergistically to preserve, support and regenerate one another in combating

oxidative stress in the body.

To test this theory, "harmanex+sponsored a study in collaboration with three maor - universities to test

s)in carotenoid levels as an indicator of the overall health of the antioxidant networ) in over 00 healthy

subects. pecifically, the researchers investigated correlations between s)in carotenoids1measured non&invasively by 'aman spectroscopy "harmanex %io"hotonic canner$1and blood serum antioxidants

vitamins C, E, and carotenoids by !"#C$, as well as urinary isoprostanes, which are widely regarded as

the best measure of oxidative stress in the body.

The results of this study confirmed prior research showing strong correlations between s)in and blood

carotenoids. Most importantly, however, the present study showed highly significant correlations between

s)in carotenoids %io"hotonic canner$ and other blood antioxidants. 2n addition, a highly significant

inverse correlation between s)in carotenoids and oxidative stress urinary isoprostanes$ was observed.Together these results confirm that the %io"hotonic canner is the best non&invasive indicator of overall

antioxidant status in the body, as well as a good indicator of overall oxidative stress.

2n addition, the study demonstrated that s)in carotenoids as measured by the canner, are a more accurateindicator of average fruit and vegetable consumption than blood carotenoids. "reviously, blood carotenoids

had been viewed as the gold standard indicator of fruit and vegetable inta)e.

This study will be submitted for publication at a maor scientific meeting as well as in a peer&reviewedournal. pecific results and further information will be released in these publications.