Clinical Pharmacology, F. Hoffman-La Roche · 2018-06-07 · Clinical Pharmacology, F. Hoffman-La...
Transcript of Clinical Pharmacology, F. Hoffman-La Roche · 2018-06-07 · Clinical Pharmacology, F. Hoffman-La...
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Application of a mechanistic, systems model of lipoprotein
metabolism and kinetics (LMK) to target selection and
biomarker identification in the reverse cholesterol transport
(RCT) pathway
James Lu & Norman MazerClinical Pharmacology, F. Hoffman-La Roche
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FROM EPIDEMIOLOGY TO
TARGETS
Relationship of cardio-vascular risk to cholesterol levels
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HDL-C and cardio-vascular risk
• Association of cholesterol levels with coronary heart disease
– Total-C = non-HDL-C + HDL-C
The Emergent Risk Factors Collaboration, JAMA ‘09
“Good” cholesterol“Bad” cholesterolunadjusted
adjusted for
other risk
factors
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Reverse Cholesterol Transport: linking HDL-C to
cardio-vascular risk
• Reverse cholesterol transport (RCT): cholesterol removal from peripheral
tissues (e.g., macrophages) back to the liver, mediated by HDL particles
HDL-C = RCT input rate
clearance of HDL−C
GI tract
&
Liver
Peripheral
tissues
Plaque
Forward Cholesterol Transport:
Non-HDL particles
Reverse Cholesterol Transport:
HDL particles
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Can the promise of HDL-C raising therapies be
fulfilled?
• Unexpected failures of HDL-C raising therapies
– CETP inhibitors (Torcetrapib, Dalcetrapib)
– Niacin (HPS2-THRIVE)
• Targeting HDL-C levels: mis-understanding of the connection between
HDL-C and CV risk?
– HDL functionality rather than quantity: e.g., RCT rate
• What conclusions should be drawn for other targets in the pathway?
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MODEL DEVELOPMENT &
CALIBRATION
Pathway representation & quantification
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Schematic diagram: targets in pathway
-HDL
C
C
ABCA1CE
C
CE
C
Fusion
Nascent Disc Nascent Sphere
LDL-CE VLDL-CE
HDL Remodeling Flux
ApoA-ISynthesis
SRB1
CETP
Lipid-poor ApoA-I
Target 1
Target 2
Target 3
Target 4
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Model calibration
• Bayesian methodology: maximum a posteriori (MAP)
– Prior estimates (𝑘𝑝𝑟𝑖𝑜𝑟) + calibration data (𝑑) → posterior values (𝑘𝑀𝐴𝑃)
– Nonlinear least squares minimization:
min𝑘
𝑘 − 𝑘𝑝𝑟𝑖𝑜𝑟𝑇𝐶𝑘
−1 𝑘 − 𝑘𝑝𝑟𝑖𝑜𝑟 + (𝐺 𝑘 − 𝑑)𝑇 𝐶𝑑−1 (𝐺 𝑘 − 𝑑) → 𝑘𝑀𝐴𝑃
Where, 𝑘𝑝𝑟𝑖𝑜𝑟: prior parameter estimate;
𝐶𝑘 and 𝐶𝑑: covariances for parameters and data (with adjustments);
𝐺 𝑘 : model simulation in reproducing data 𝑑.
• Prior estimates of parameters
– Number of literature references: 11
– Informative priors: 14/16 parameters
• Calibration data
– Number of literature references: 8
– Number of data values: 15
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Posterior parameter values and uncertainty
• Fold changes in posterior values and estimates of confidence intervals
using Fisher Information Matrix
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Correlation analysis using a virtual population
• Relationships between biomarkers can be studied within a virtual population
• Model offers an explanation for the association between HDL-C and CV risk
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TARGET MODULATIONAssessment of HDL-C raising targets
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Model validation on targets of interest
• ApoA-I and ABCA1 are important targets in RCT pathway
– Validate model by simulating heterozygous & homozygous mutations
Set ApoA-I synthesis rate
& ABCA1 activity to 50%
and 0% of normal
ApoA-ISynthesis
Lipid-poor ApoA-I C
ABCA1 C
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Comparison of Target Modulations
• Predicted HDL-C & RCT changes: CETP inhibition vs ABCA1 up-regulation
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Model predictions on lipoprotein biomarkers
• The changes in RCT rate and biomarkers depend on the MoA
CETP inhibitors: Schwartz et al, NEJM ‘12; Clark et al, ATVB ’04; Cannon et al, NEJM ‘10
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OPTIMIZE DOSAGE REGIMEN Contextualize drug mechanism within disease biology
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Bolus infusion of ApoA-I
Simulation of ApoA-I infusion therapy
• Optimize formulation & dose schedule: maximize cholesterol removal
ApoA-ISynthesis
Lipid-poor ApoA-I C
ABCA1 C
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BIOMARKER IDENTIFICATIONModel-based approach for
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The right biomarker for ABCA1 activity
• In-vivo, whole-body ABCA1 activity is difficult to assess experimentally
• Infer the most effective lipoprotein-based surrogate for ABCA1 activity
Poor indicator of ABCA1 activity Robust marker of ABCA1 activity
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Conclusions
• Mechanistic modeling:
– Integrates state-of-art biology with prior experimental & observational
data
• Leverages the explosion in biological data
– Contextualizes drug mechanisms within the disease biology
• Quantifies effect of drugs on disease progression
– Broad potential impacts within drug discovery & development
• Assess targets, biomarkers, dosage; reconfirm MoA, …
• LMK model:
– Quantifies linkage between HDL-C and RCT
– Provides explanation for failure of CETP inhibition
– Identifies better targets for impacting cardio-vascular risk
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Acknowledgements
• Project consultants: Katrin Huebner, Eliot Brinton and M. Nazeem Nanjee
• Roche Clinical Pharmacology: Valerie Cosson, Nicolas Frey, Ronald Gieschke,
Cheikh Diack, Candice Jamois, Eliezer Shochat, Franziska Schaedeli Stark,
Annabelle Lemenuel, Dean Bottino, Joy Hsu, Vishak Subramoney, Christophe Le
Gallo, Daniel Serafin, Vincent Buchheit, Yumi Fukushima, Dietmar Schwab,
Bernhard Mangold, Michael Derks, Conni Weber, Bruno Reignier, Jean-Eric Charoin
and Richard Peck
• Roche Cardio-Vascular & Metabolism DTA: Matthew Wright, Eric Niesor,
Cyrille Maugeais, Hans-Joachim Schoenfeld, Gregor Dernick, Philippe Ferber,
Everson Nogoceke, Thomas Schindler and Laurent Essioux
• Genentech: Kapil Gadkar, Saroja Ramanujan, Srikumar Sahasranaman, John
Davis
• Roche Postdoc Fellowship Committee: Klaus Mueller
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Doing now what patients need next