Clinical Pathological Conference Dr. David Gonzales May 5, 2006.
-
Upload
ralf-lewis -
Category
Documents
-
view
213 -
download
0
Transcript of Clinical Pathological Conference Dr. David Gonzales May 5, 2006.
![Page 1: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/1.jpg)
Clinical Pathological Conference
Dr. David Gonzales
May 5, 2006
![Page 2: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/2.jpg)
CC: Abdominal pain x 2 months
PMH: 49 y/o AAM with h/o abdominal pain x 2 months.
Pain is epigastric, constant, severe, disturbs sleep, no radiation, worse with meals and associated with early satiety.
Associated with nausea, had emesis x 2 two months ago.
He reports black stools for 2 weeks but denies BRBPR.
He has had poor PO intake and reports losing > 20 lbs over 2 months.
He also c/o constipation.
No abdominal distention. No jaundice or acholic stools.
He denied fever or chills but did say he “felt hot” and had some sweats.
![Page 3: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/3.jpg)
He went to the ER at another facility 2 months ago and was given an unknown medication after X rays and sonogram of the abdomen were done; reportedly negative.
His pain worsened and he developed back pain radiating down both lower extremities. He returned to the ER a few weeks later and was given hydrocodone/APAP and a PPI and asked to f/u with a PCP.
His PCP continued the PPI but pain persisted. He then developed a new R flank pain, pain radiating down R LE worsened and he complained of R LE weakness.
He went back to the ER at the other hospital and after further workup was transferred to our hospital.
![Page 4: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/4.jpg)
PMHx: No PCP H/o MVA x 2 with R elbow #.
PSHx: none
Allergies: NKDA
Medications: Pantoprazole 40 mg po qd Hydrocodone/APAP as needed
Social History: Single. Lives with his parents. Will not disclose sexual orientation but has not been sexually active for > 1 year, no h/o STDs. Occasional tobacco-cigars for 3 years, occasional ETOH, denies recreational drug use.
Family History: Mother with DM, HTN; Father with HTN
ROS: Positive for fatigue, weakness, “feeling hot”, sweats and weight loss as above. Also reports hiccups and R LE weakness , otherwise negative.
![Page 5: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/5.jpg)
PHYSICAL EXAM
Gen: emaciated 49 y/o AA male
BP 138/92. HR 111, RR 18, Wt 68.5 kg, T-afebrile
HEENT: Normocephalic, atraumatic, PERRL, EOMI, OP clear, slightly dry.
Neck- Supple. No JVD, bruit, LAD, thyromegaly.
Lungs: CTAB
Cv: RRR. S1S2+, no murmur, gallop, rub. Tachycardia+
Abd: Periumbilical and RUQ fullness, Prominent veins above the umbilicus, NABS+. Liver edge palpable, firm, nontender-span 11 cms. Ill defined mass about 8x8 cm palpable in the periumbilical area, lower right border rounded, other borders poorly defined, firm, non tender. No splenomegaly. No shifting dullness.
Rectal- Normal sphincter tone. Brown, guaiac –ve stool
Neuro: CNS II-XII grossly intact, DTRs 2/4,, sensation intact, F-to-N intact. Strength 5/5 b/l UEs, RLE prox 3+/5, distal 4/5, LLE prox 4/5, distal 4+/5. Gait not evaluated
Skin: No rash.
Lymphatic: No significant LAD; Ext: Warm, perfused, no LEE.
.
![Page 6: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/6.jpg)
Labs 8.3 \ 8.9 / 540 / 27.1 \ N82%, L8%, M6%, E1%,Bands1%, Metamyelocytes 2% Fe <20 ug/dL, ferritin 316 ng/ml, transferrin 171mg/dL, % sat< 8. MCV was 78%, his stool occult blood was initially negative but turned positive
3 days later. RDW 14.9%
129 │98 │23 / 73 Ca 8.6 mg/dL 4.6 │24 │1.7 \
Tp 8.1 g/dL Alb 3.4 g/dL Glob 3.7g/dL AST 78 U/dL ALT 79 u/dL AlkP 686 u/dL Tbili 0.6 mg/dL SPEP-protein 6.8-high Alpha 1 globulin 0.32, low albumin
FENA< 1 UA-wnl
Lipase 1391 U/L, amylase 302 U/L, Uric acid 14.6 mg/dL
LDH 2952 U/L , CEA< 0.5 ng/ml, CA 19-9 – 130 U/ml
![Page 7: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/7.jpg)
Fecal H. pylori Antigen -ve; HIV status +ve; CD4-absolute 110, %- 29% The creatinine and sodium normalized with hydration The alkaline phosphatase dropped to the 190s later.
More tests were ordered…..
![Page 8: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/8.jpg)
Radiology:
CXR: Small to moderate left pleural effusion with left basilar atelectasis.
CT Spine- Disc disease L5-S1-no fracture.
Other imaging was obtained…..
![Page 9: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/9.jpg)
CT Abdomen/Pelvis
Massive retroperitoneal adenopathy, Moderate R
hydronephrosis, Prominent gastric folds/thickened gastric
wall.
![Page 10: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/10.jpg)
A diagnostic procedure was performed….
![Page 11: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/11.jpg)
CPC
David Gonzales
Presbyterian Hospital of Dallas
May 5, 2006
![Page 12: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/12.jpg)
Case presentation
49 year old male with epigastric pain x 2 months Constant, severe
Early satiety
20 pound weight loss, sweats, “feeling hot”
![Page 13: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/13.jpg)
Management of dyspepsia Unintended weight loss Persistent vomiting Dysphagia/Odynophagia Anemia Hematemesis Palpable mass iron deficiency anemia Family history of upper
gastrointestinal cancer Previous gastric surgery Jaundice
![Page 14: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/14.jpg)
Case presentation
Sonogram (-)
No improvement with PPI
Pain extends to right flank and unilateral lower extremity weakness develops
Remainder of history not incredibly helpful
![Page 15: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/15.jpg)
Differential Diagnosis
Tumor Lymphoma Biliary or other sites in GI tract
Infection with atypical organism Gastritis/gastropathy
![Page 16: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/16.jpg)
Physical Exam
Mild tachycardia Emaciated Periumbilical/RUQ fullness with firm, 8cm
mass; liver palpable Right leg 3/5 prox, 4/5 distal; left leg 4/5;
sensation and reflexes normal
![Page 17: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/17.jpg)
Labs
Microcytic anemia Ferritin 316 (28-365) Iron<20, transferrin 171, 8% saturation Mild thrombocytosis also argues for iron
deficiency Mild hyponatremia and renal insufficiency
which corrected with hydration
![Page 18: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/18.jpg)
Labs
Alkaline Phosphatase about 5 x normal with normal bilirubin Mild transaminitis
Lipase and amylase elevated Ca 19-9 moderately elevated
LDH and uric acid very elevated
![Page 19: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/19.jpg)
![Page 20: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/20.jpg)
SPEP
A screening test for plasma cell disorders A clone secretes a
homogenous (M for monoclonal) protein
If M protein is found, it must be characterized by immunofixation
This patient had high alpha-1 globulin and low albumin
![Page 21: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/21.jpg)
And he’s got AIDS…
CD 4 = 110 Abdominal pain is common in HIV
Opportunistic infection Regular stuff (gastritis, cholecystitis, etc) Medication related Malignancy
![Page 22: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/22.jpg)
55 yo AA male with AIDS and
Abdominal pain with fever, weight loss, and sweats
Abdominal mass Iron-deficiency anemia Markers of high cell turnover Pancreaticobiliary abnormalities
![Page 23: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/23.jpg)
Imaging
CXR: small left pleural effusion with atelectasis
CT Abdomen/Pelvis Massive retroperitoneal adenopathy
Moderate R hydronephrosis Prominent gastric folds No info on liver, pancreas, or kidney: assume
normal
![Page 24: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/24.jpg)
Retroperitoneal Adenopathy
Retroperitoneal fibrosis Testicular Cancer Renal Cell Carcinoma Opportunistic infection Lymphoma
![Page 25: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/25.jpg)
Enlarged Gastric Folds
Menetrier’s disease Foveolar hyperplasia in
the body and fundus of the stomach
Symptoms include pain, asthenia, anorexia
Often have hypoalbuminemia secondary to protein-losing enteropathy
![Page 26: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/26.jpg)
Enlarged Gastric folds
Zollinger-Ellison (gastrinoma) High gastrin output causes acid hypersecretion
ulcers, primarily in the duodenum and distally Often associated with diarrhea (3/4 of pts) Weight loss only present in 17% MEN 1
![Page 27: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/27.jpg)
Enlarged Gastric Folds
H. Pylori-associated gastritis
Anisakiasis
Adenocarcinoma
Lymphoma GI vs. HIV-related
![Page 28: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/28.jpg)
Gastrointestinal lymphoma
Defined as localized disease in the GI tract or presentation predominantly in the GI tract
Role of MALT Spread from adjacent nodes
Diffuse large B-cell lymphoma also seen
![Page 29: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/29.jpg)
![Page 30: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/30.jpg)
HIV and malignancy
Increased incidence similar to transplant recipients
Length and degree of immunosuppression likely play roles
Role of HIV and other viruses including EBV
![Page 31: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/31.jpg)
HIV and lymphoma
Risk increases directly as CD4 count drops 3 General types
Primary CNS lymphoma Primary effusion lymphoma Non-Hodgkin’s lymphoma
![Page 32: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/32.jpg)
HIV and systemic lymphoma
Tend to be aggressive High proliferation Spontaneous cell death
2 main histologic types Diffuse large B cell Burkitt’s or Burkitt’s-like
![Page 33: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/33.jpg)
Burkitt’s lymphoma
3 types Endemic (African) Non-endemic (American) Immunodeficient
Translocation of C-myc is important
![Page 34: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/34.jpg)
![Page 35: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/35.jpg)
![Page 36: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/36.jpg)
![Page 37: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/37.jpg)
To summarize
55 year old male with AIDS Abdominal pain from a large, fast-growing
retroperitoneal mass B symptoms (probably) Evidence of high cell turnover (uric acid and LDH LE motor weakness and R hydronephrosis
Suspect nerve root and ureteral compression from adenopathy
![Page 38: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/38.jpg)
Summary
Diagnostic test: lymph node biopsy EUS?
![Page 39: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/39.jpg)
Diagnosis
AIDS-associated NHL, favor Burkitt’s Rapid presentation High cell turnover Rare
![Page 40: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/40.jpg)
M.C.
Diffuse high grade B-cell lymphoma, Burkitt- like morphology
2/1/06 Retroperitoneal core biopsy2/1/06 Bone marrow biopsy2/2/06 Duodenal mass biopsy
Gastric mass biopsy2/2/06 CSF cytology
![Page 41: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/41.jpg)
M.C.
Pathology
Diffuse infiltrate B-cells (CD20+) Uniform intermediate size nuclei Fine chromatin, nucleoli present Basophilic cytoplasm with vacuoles Numerous mitoses, admixed histiocytes
![Page 42: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/42.jpg)
![Page 43: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/43.jpg)
![Page 44: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/44.jpg)
![Page 45: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/45.jpg)
![Page 46: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/46.jpg)
![Page 47: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/47.jpg)
M.C.
Immunophenotype
Positive: CD20, CD79, CD10, Bcl-6, CD38Negative: CD34, CD117, CD3, Tdt, Bcl-2,
CD138, CD5, CD23, CD56
Light chain restriction: KappaKi-67 proliferative index = high (> 90%)
![Page 48: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/48.jpg)
M.C.
Diagnosis Diffuse high grade B-cell lymphoma, Burkitt-
like morphology Definitive diagnosis pending FISH studies for
c-myc translocation c-myc FISH results
1st lab: negative 2nd lab: positive
![Page 49: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/49.jpg)
LSI IGH/MYC, CEP 8 LSI MYC break apart
FISH
![Page 50: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/50.jpg)
Classic Burkitt’s lymphoma
Morphology Diffuse, monomorphic cells Interspersed macrophages – “starry sky” Neoplastic nuclei are uniform, round
intermediate size Multiple (2-5) small distinct nucleoli – uniform Moderate amount basophophilic vacuolated
cytoplasm Frequent mitoses, apoptotic bodies
![Page 51: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/51.jpg)
Classic Burkitt’s lymphoma
Immunophenotype
B-cell – CD20, CD19, CD22 Surface monoclonal Ig Positive: CD10, CD43 Negative: TdT, CD34, Bcl-2, CD138
![Page 52: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/52.jpg)
Burkitt Lymphoma (BL)
Rare in non-immune depressed < 1% of NHL
HIV (+) – 1000 fold incidence of BL
![Page 53: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/53.jpg)
Lymphoma in HIV/AIDS
NHL in 4-10% AIDS patients Relative risk for NHL 60-200 fold 10% of all NHL in USA NHL affects all AIDS groups equally HL relative risk 10 fold in AIDS
![Page 54: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/54.jpg)
Lymphoma in HIV/AIDS HIV virus is not directly lymphomagenic Deficient immune surveillance/cellular
immunity HIV associated infections
Activation/proliferation B-cells B-cell genetic instability: mutations, deletions,
translocation of tumor suppressor genes/oncogenes
Clonal expansion of genetically altered B-cells B-cell NHL
![Page 55: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/55.jpg)
Lymphoma in HIV/AIDS
Clinical CD4/CD8 < 0.5 CD4 lymphocytes < 100 mm3
Extra nodal location (60%) GI, CNS, liver, bone marrow Advanced stage at presentation Clinically aggressive Short survival
![Page 56: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/56.jpg)
Lymphoma in HIV/AIDS
Pathology Aggressive histologic subtypes Diffuse growth pattern High proliferation rate
Mitoses Ki-67 immunostain
Frequent necrosis Cell debris/macrophages
![Page 57: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/57.jpg)
Lymphoma in HIV/AIDSPathology
Diffuse large B-cell lymphoma (70%) Immunoblastic Pleomorphic (Burkiitt’s like/atypical Burkitt’s) (Centroblastic)
Burkitt’s lymphoma (30%) Classic Burkitt’s like/atypical Burkitt’s
![Page 58: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/58.jpg)
Lymphoma in HIV/AIDSPathology
Rare subtypes (< 1%) Primary effusion lymphoma
Oral cavity plasmablastic lymphoma
![Page 59: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/59.jpg)
Lymphoma AIDS/HIV
BL Large cell lymphoma
Age Younger Older
CD4 level Higher Lower (< 100 mm3)
Location Nodal Extra-nodal
AIDS
SyndromeOften (-)
Usually present - advanced
![Page 60: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/60.jpg)
NHL - AIDS
EBV Infection
40-50% Burkitt’s lymphoma 70% Diffuse large B cell lymphoma
![Page 61: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/61.jpg)
Burkitt’s Lymphoma
3 clinical variants Endemic Sporadic Immunodeficiency associated
Common antecedents Immunodeficiency Antigenic stimulation
Genetic translocation/activation of MYC gene at chromosome 8q26
![Page 62: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/62.jpg)
Burkitt LymphomaMolecular Genetics
Translocation c-myc gene →activation Increased c-myc protein
Transcription factor for many genes Increased cell proliferation
![Page 63: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/63.jpg)
Burkitt LymphomaMolecular Genetics
Balanced translocation of c-myc oncogene on chromosome 8q24 into Ig heavy chain gene 14q32 Kappa light chain gene 2q11 Lambda light chain gene 22q11
![Page 64: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/64.jpg)
Burkitt’s lymphoma
Morphology
3 morphologic variants Classic Plasmacytoid Burkitt Atypical Burkitt/Burkitt-like
All BL variants High proliferation rate/growth fraction MYC translocation
![Page 65: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/65.jpg)
Burkitt’s lymphoma
Morphology BL variants (non-classic)
Variable nucleoli Variable nuclear size, shape, chromatin
texture Cytoplasm +/- plasma cytoid
Histologic diagnosis of variants low reproducibility
![Page 66: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/66.jpg)
Burkitt/Burkitt-like LymphomaMorphology
Cases in non-AIDS/HIV children “Sporadic” Burkitt in USA Classic Burkitt morphology, uniform
immunophenotype Simple, non-complex c-myc translocation Prognosis excellent
![Page 67: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/67.jpg)
Burkitt/Burkitt-like LymphomaMorphology
Cases in immunocompetent adults are (2/3) Diffuse large B-cell lymphoma
c-myc negativePrognosis similar to DLBCL
(2/3) True atypical Burkitt/Burkitt-like lymphoma
c-myc positiveComplex c-myc translocationsExtremely poor prognosis
Classic Burkitt very rare > 20-25 y.o.
![Page 68: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/68.jpg)
Diffuse Large B-cell Lymphoma
Morphologic subtypes Centroblastic Immunoblastic Pleomorphic/anaplastic T-cell/histiocyte
Morphologic subtypes not reproducible Marginal prognostic significance
![Page 69: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/69.jpg)
Diffuse Large B-cell LymphomaPrognosis
postulated cell of origin Germinal center Post germinal center/activated
2 major subtypes by gene expression Germinal center immunophenotype
Bcl-6/CD-10 immunostain (+) Better prognosis/intermediate grade
Activated/post-germinal center immunophenotype Express activation markers MUM1, CD-138 Worse prognosis/high grade
![Page 70: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/70.jpg)
NHL-AIDS/HIVHAART
Preliminary Data Incidence NHL declined Longer history of AIDS diagnosis Less frequent 1° CNS lymphoma Histology shift to intermediate grade NHL Fewer high grade large B-cell lymphomas
Large B-cell lymphoma (activated) Improved survival
Diffuse large B-cell lymphoma
![Page 71: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/71.jpg)
NHL-AIDSPost-HAART
Burkitt’s lymphoma no improvement in prognosis
Unresolved issues: Prognostic significance of BL variants
Simple vs complex c-myc variants Improved survival of DLCL
Less frequent activated subtypes Relative increase in germinal center subtype
Most appropriate therapy for BL
![Page 72: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/72.jpg)
Burkitt-like/Atypical Burkitt’s Lymphoma Diffuse, high mitotic rate Nuclei ≥ size of macrophage nuclei Amphophilic/plasmacytoid cytoplasm Variable
nuclear size nuclear shape Nucleoli Imunophenotype
FISH confirmation of c-myc translocation or complex c-myc signal
Extremely aggressive clinical course** HIV/AIDS* Sporadic adults
![Page 73: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/73.jpg)
Burkitt-like/Atypical Burkitt’s Lymphoma
Morphology
FISH negative for c-myc translocation/complex pattern
Not Burkitt or/atypical Burkitt lymphoma Diffuse B-cell lymphoma NOS Prognosis similar to diffuse large B-cell
lymphoma
![Page 74: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/74.jpg)
Burkitt Lymphoma Endemic
100% EBV Morphology classic C-myc simple, non-complex translocation
Sporadic < 30% EBV Morphology: classic, atypical C-myc translocation: simple, complex
Immunodeficiency 40-50% EBV Morphology: classic, atypical C-myc translocation: simple, complex
![Page 75: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/75.jpg)
Burkitt Lymphoma
USA children – homogenous entity Classic morphology Classic immunophenotype Classic genotype – single c-myc translocation Classic clinical course Good prognosis with modern treatment
![Page 76: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/76.jpg)
![Page 77: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/77.jpg)
![Page 78: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/78.jpg)
![Page 79: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/79.jpg)
![Page 80: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/80.jpg)
![Page 81: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/81.jpg)
Mature B-cell Compartments
Pre-germinal center (virgin) B-cells No somatic mutation IgV, Bcl-6
Germinal center B-cells Somatic hypermutation Bcl-6 +/or somatic hypermutation IgV
Post-germinal center B-cells Somatic hypermutation Bcl-6 +/or IgV Expression of activation markers MUM-1, CD-138
![Page 82: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/82.jpg)
Burkitt-like/Atypical Burkitt Lymphoma Adult, median 68 (20-90) Extra nodal ~ 50% Variable:
Immunophenotype Genotype
1/3 c-myc translocation Single translocation Complex
Prognosis related to c-myc translocation (+) c-myc – very poor prognosis (< 1 yr) (-) c-myc similar to DLBL
![Page 83: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/83.jpg)
High grade B-cell LymphomaBurkitt-like/Atypical Burkitt Many but not all morphologic features of
classic BL Shared features:
High mitotic rate Dispersed macrophages (starry sky)
Distinguishing features Greater/variable nuclear size/shape
Not reproducible entity No standardized/reproducible diagnostic
criteria
![Page 84: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/84.jpg)
![Page 85: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/85.jpg)
High grade B-cell LymphomaBurkitt-like/Atypical Burkitt
Most cases in adults are ??? Diffuse large B-cell lymphoma Atypical Burkitt’s/Burkitt’s like Diffuse high grade B-cell lymphoma
![Page 86: Clinical Pathological Conference Dr. David Gonzales May 5, 2006.](https://reader036.fdocuments.in/reader036/viewer/2022070411/56649f4e5503460f94c6eddf/html5/thumbnails/86.jpg)
Burkitt’s LymphomaEBV Infection
Endemic 100% EBV Morphology – classic
Sporadic 30% EBV Morphology – classic, atypical
Immunodeficiency 40-50% EBV Morphology – classic, atypical