Clear Whey

Doctor’s Best Clear Whey Protein Isolate is a water-soluble whey protein isolate powder that delivers the highest quality whey protein in a clear solution. It is produced by utilizing a proprietary filtration process that isolates native whey proteins in a highly concentrated form to provide maximum functionality and exceptional nutritive value, along with all the essential amino acids (EAAs) including the 3 BCAAs (branched-chain amino acids: Leucine, Isoleucine, and Valine) crucial for muscle growth.

While it is well-known that taking whey protein with BCAAs before any workout can help physical performances, Doctor’s Best Clear Whey Protein Isolate is the ideal whey protein isolate loaded with the complete spectrum of all three BCAAs that can be taken during your workout thanks to the exceptional clean taste it gives. By consuming BCAAs during training, it will help reducing the muscle breakdown, promoting endurance and refueling the lost energy and nutrition during the workout. As a result of its patented filtration process, Doctor’s Best Clear Whey Protein Isolate offers the utmost clean formula that is easily mixed and absorbed by the body and can be taken by competitive and recreational athletes. While ingestion of proteins is important for maintenance of a variety of body functions, whey protein has become a significant asset in medical field to improve bone, cardiovascular, and immunity health for the general population, from the young to the eldest.*1-5 In particular, in the search of maintaining a healthy active lifestyle, whey protein supplementation for older individuals has become an essential health nutritive value to fight against loss of muscle mass.*6-7

BENEFITS• Provides a very pure form of whey protein isolate with

more than 90% protein with minimal lactose (<1%) and virtually no fat*

• Provides a complete spectrum of all nine essential amino acids including the 3 BCAAs (Branched Chain Amino Acids) necessary for increasing muscle growth and lean body mass and decreasing muscle breakdown and soreness*

• Has been specially formulated to be taken during workout for optimal results (it can also be taken before and after)*

• Helps improve overall performance by increasing * These statements have not been evaluated by the Food and Drug Administration.

This product is not intended to diagnose, treat, cure or prevent any disease.

Cherry, 19.5 OZ (1.2 LB) 546gSupplemental Facts

strength and muscle recovery and reducing exercise-induced fatigue*

• Provides optimal protein intake for the ageing population*

• 100% Hydrolyzed Whey Protein*

INGREDIENTS

Protein Isolate

* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

EXTENDED BENEFITSProvides a very pure form of whey protein isolate with more than 90% protein with minimal lactose (<1%) and virtually no fat.Whey protein is a type of milk protein produced as a byproduct of cheese production and the term “whey” refers to the complex mixture that is separated from the liquid remaining after milk has been curdled and strained. Although, whey protein is well-known worldwide as a superfood, building muscle and improving exercise endurance, it has been used in various products such as in infant formulas, food supplements, beverages, and in different applications of medical field to meet a variety of health goals for people of all ages.*7-8 Historically, the intake of whey in the form of liquid can be dated back to around 2500 years ago when Hippocrates recommended some drinks to enhance the immune system, power, and the muscle growth rate of the body.*9

However, based on current research, not all whey proteins are equal. Indeed, whey is available in three major forms: Whey Protein Concentrates, Whey Protein Isolates, and Whey Protein Hydrolysates. When it comes to building muscle growth and improving overall physical endurance, whey protein isolates are the form of supplementation preferred. Whey isolate undergoes different processing than whey concentrate, which results in the isolate containing more protein with less carbs and fat per serving.2 Moreover, whey protein isolates are both easily digested and have excellent metabolic efficiency. They contain the highest percentage of protein source. Doctor’s Best Clear Whey Protein Isolate uses a unique process of filtration allowing to produce a whey isolate powder containing 90% or more protein by weight. In addition to having lower total carbohydrate content, whey isolate also has lower lactose content. This means also that it may be a better choice for those who are lactose intolerant. In addition, Doctor’s Best Clear Whey Protein Isolate contains all the essential amino acids including the three branched-chain amino acids known to stimulate protein synthesis and muscle growth, which is important in muscle building and muscle retention.*10

Has been specially formulated to be taken during workout for optimal results (it can also be taken before and after).It is well-known that whey protein supplements are beneficial to athletes and bodybuilders in promoting muscle growth and improving overall physical endurance and are overall the best protein sources to take when compared to other types of protein supplements (casein and soy).*11 Traditionally, dietary protein supplements are ingested before or after physical exercise. But based on recent new studies, the potential to modulate protein synthesis by taking whey protein supplementation during exercise (or intra-workout) may also be beneficial to the body. Doing so, this could increase muscle protein synthesis rates, stimulate net muscle protein augmentation, and minimize protein degradation, thereby lead to greater gains in fat-free mass and overall enhance physical endurance.*12-13 Doctor’s Best with Clear Whey IsolateTM has a unique formulation that provides a product clear in solution, clean in taste, and without the texture of common whey protein allowing athletes and any individuals looking to increase their protein intake to ingest whey protein isolates during workout for optimal results.*

Provides a complete spectrum of all nine essential amino acids including the 3 BCAAs

(Branched Chain Amino Acids) necessary for increasing muscle growth and lean body mass and decreasing muscle breakdown and soreness.*

Helps improve overall performance by increasing strength and muscle recovery and reducing exercise-induced fatigue.*Numerous studies in animals and humans have shown that whey proteins can have tremendous nutraceutical potential for athletes.*7,14 They contain amino acids to support body

Peach Mango, 18.9 OZ (1.17 LB) 529.2gSupplemental Facts

* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

composition and all the essential amino acids that the body cannot synthetize to enhance athletic performance.* In particular, with its unique proprietary filtration process, Clear Whey Protein Isolate is able to isolate native whey proteins in a highly concentrated form to provide maximum functionality, delivering consequently the purest proteins including all the EAAs and the three BCAAs (leucine, isoleucine, and valine) vital for muscle growth, strength and recovery, and fat loss.*15-18

Other studies have also linked a positive relationship between BCAAs intake and the reduction of muscle damage associated during endurance exercise.*19 With respect to lean body mass, Cermak et al. performed a meta-analysis that examined the impact of protein supplementation on changes in strength and fat-free mass. Data from 22 separate published studies that included 680 research participants were included in the analysis. These authors concluded that protein supplementation demonstrated a positive effect of fat-free mass and lower-body strength in both younger and older participants.*20 Similarly, in 2018, Morton et al. performed a meta-review and meta-regression involving 49 studies and 1863 participants and found that protein supplementation can contribution to increases in fat-free mass.*21

Recent studies and scientific reviews have highlighted that BCAAs, particularly Leucine, has critical biochemical actions involved in stimulating protein synthesis, inhibiting protein degradation, augmenting the activity of protein and availability of specific eukaryotic cell initiation factors.22-26 In particular, leucine is the main BCAA that modulates the function of proteins in gene translation via activating of rapamycin complex-1 (mTORC1), the mTOR signaling pathway known to be involved in cellular growth, muscle protein synthesis and muscle repair.27-31

While BCAAs are key to increase stimulation of mTORC1 activity, the ingestion of BCAAs alone, without the concurrent ingestion of other EAAs, intact protein or other macronutrients, does not maximally stimulate muscle protein synthesis following exercise despite the stimulation of mTORC1 translation initiation pathways.*18 Doctor’s Best Clear Whey Protein Isolate will deliver all of these essential nutraceutical organic compounds for the best health and physical outcomes.*

Provides optimal protein intake for the ageing population*Maintaining an independent healthy lifestyle is one of the top priorities for the elderly population. Unfortunately, one of the major drawbacks of growing older is the loss of skeletal muscle mass, strength and function, and the gain of body fat that progressively occur due to body composition shifts. Research suggests that one of the potential mechanisms contributing to the loss of skeletal muscle mass is reduced muscle protein synthesis, which was shown to decline by 3.5% per decade.32

Scientific data suggest that increasing dietary protein intake may counterbalance muscle loss in older individuals due to the increased availability of amino acids, which stimulate muscle protein synthesis.*33-36

In addition, based on recent metabolic and epidemiological studies, there is sufficient evidence that protein intake higher than the current dietary recommendations (0.8 g/kg/day) is beneficial for most older adults. Higher protein intakes are associated with increased muscle protein synthesis, which is correlated with increased muscle mass and function. This, in turn, is linked to improved physical function.*37 Other studies have been conducted in the older population to show the positive effect of taking whey protein supplements on reducing

exercise-induced strength loss caused by muscular fatigue from working out.*38-40

CLINICAL STUDIESA study investigated the ability of whey-protein and leucine supplementation in enhancing physical, body composition, and cognitive performance. Thirty participants completed a modified Air Force fitness test, a computer-based cognition test, and a dual-energy X-ray-absorptiometry scan for body composition before and after supplementing their daily diet for 8 weeks with either 19.7 g of whey protein and 6.2 g leucine (WPL) or a calorie-equivalent placebo (P). Bench-press performance increased significantly from Week 1 to Week 8 in the WPL group, whereas the increase in the P group was not significant. Push-up performance increased significantly for WPL group, and P

Fruit Punch, 18.9 OZ (1.17 LB) 529.2gSupplemental Facts

group showed a nonsignificant increase. Total mass, fat-free mass, and lean body mass all increased significantly in the WPL group but showed no change in the P group. No differences were observed within or between groups for crunches, chin-ups, 3-mile-run time, or cognition. The authors conclude that supplementing with whey protein and leucine may provide an advantage to people whose performance benefits from increased upper body strength and/or lean body mass.*15

A pilot double-blind cross-over designed study aimed to test whether BCAA supplementation enhances exercise capacity and lipid oxidation in glycogen-depleted subjects. Volunteers were randomly assigned to either the BCAAs (300 mg/kg/day) or the placebo (maltodextrin) for 3 days. On the second day, subjects were submitted to an exercise-induced glycogen depletion protocol. They then performed an exhaustive exercise test on the third day, after which time to exhaustion, respiratory exchange ratio (RER), plasma glucose, and other parameters were determined. Results showed BCAAs supplementation promoted a greater resistance to fatigue when compared to the placebo (+17.2%). Moreover, subjects supplemented with BCAAs showed reduced RER and higher plasma glucose levels during the exhaustive exercise test. In conclusion, the authors suggested that BCAAs supplementation may increase resistance to fatigue and enhances lipid oxidation during exercise in glycogen-depleted subjects.*16

A study investigated the effects of daily oral L-leucine ingestion on strength, bone mineral-free lean tissue mass (LTM) and fat mass (FM) of living humans during a 12-week resistance-training program. Twenty-six initially untrained men were randomly assigned to ingest either 4 g/day of L-leucine or a corresponding amount of lactose (placebo group). All participants trained under supervision twice per week following a prescribed resistance training program using eight standard exercise machines. Testing took place at baseline and at the end of the supplementation period. Results showed the leucine group demonstrated significantly higher gains in total 5-repetition max strength (sum of 5-RM in eight exercises). The percentage total 5-RM strength gains were 40.8% and 31.0% for the leucine and placebo groups respectively. The authors concluded that 4 g/day of L-leucine supplementation may be used as a nutritional supplement to enhance strength performance during a 12-week resistance training program of initially untrained male participants.*24

A study examined the effects of BCAAs supplementation on markers of muscle damage induced via a sport specific bout of damaging exercise (100 consecutive drop-jumps) in twelve trained volunteers. They were randomly assigned to a supplement or placebo group. Creatine kinase (CK), maximal voluntary contraction (MVC), muscle soreness (DOMS), and other markers of muscle damage were measured. All variables were measured immediately before the damaging exercise and at 24, 48, 72 and 96 h post-exercise. Results showed significant time effect for all variables. There were significant group effects showing a reduction in CK efflux and muscle soreness in the BCAA group compared to the placebo. Furthermore, the recovery of MVC was greater in the BCAA group. The authors concluded that BCAAs administered before and following damaging resistance exercise reduces indices of muscle damage and accelerates recovery in resistance-trained males. They concluded that BCAAs provided greater bioavailability of substrate to improve protein synthesis and thereby the extent of secondary muscle damage associated with strenuous resistance exercise.*41

A study investigated the response of myofibrillar-muscle protein synthesis to ingestion BCAAs only (i.e., without concurrent ingestion of other EAA, intact protein, or other macronutrients) following resistance exercise in ten young participants. These young athletes completed two trials, ingesting either 5.6 g BCAAs or a placebo drink immediately after resistance exercise. Myofibrillar-muscle protein synthesis was measured during exercise recovery with a primed, constant infusion of a marker and muscle biopsies pre and 4 h-post drink ingestion were collected. Blood samples were collected at time-points before and after drink ingestion. Based on the data obtained, myofibrillar-muscle protein synthesis was 22% higher in BCAAs group than placebo group. They authors

* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Green Apple, 18.8 OZ (1.16 LB) 525gSupplemental Facts

concluded that ingesting BCAAs alone can increase post-exercise stimulation of myofibrillar-muscle protein synthesis through the mTORC1 signaling.18

A study investigated the effect of protein co-ingestion with carbohydrate on muscle protein synthesis during resistance-type exercise. Ten healthy males were studied in the evening after they consumed a standardized diet throughout the day. Subjects participated in two experiments in which they ingested either carbohydrate (CHO) or carbohydrate with protein (CHO-PRO) during a 2-h resistance exercise session. Subjects received a bolus of test drink before and every 15 min during exercise. Blood and muscle biopsies were collected to assess whole body and muscle protein synthesis rates during exercise. Protein co-ingestion lowered whole body protein breakdown rates by 8.4 3.6% compared with the ingestion of carbohydrate only, and augmented protein oxidation and synthesis rates by 77 17 and 33 3%, respectively. Consequently, whole body net protein balance was negative in CHO group, whereas a positive net balance was achieved after the CHO-PRO treatment. The authors concluded that even protein co-ingestion stimulates whole body and muscle protein synthesis rates during resistance-type exercise.*12

A clinical evaluated the effect of a 12-week leucine-rich amino acid supplementation in combination with moderate training among forty-eight healthy subjects (aged 55-75 years old) who exercised for 30 minutes three times per week and received either a leucine-rich amino acid supplementation or a placebo. Before and after supplementation, volunteers performed an exhaustive eccentric exercise protocol. Many factors and markers (i.e. Maximal concentric strength, muscle soreness, creatine kinase),were assessed before exercise and after 3, 24, 48 and 72 hours. Results showed the supplementing with leucine resulted in reduced loss of strength at 0 and 3 hours after downhill walking compared with the placebo. The principle findings show that leucine-rich amino acid supplementation can counteract the negative effects of eccentric exercise. The treatment resulted in a reduction of exercise-induced strength loss.*39

The VIVE-2 Study, a Randomized, Double-Blind, Placebo-Controlled Trial, was conducted to examine the impact of Nutritional Supplementation with Physical Activity on Muscle Composition in Mobility-Limited among one hundred and forty- nine Older Adults (age: 78.5 (5.4) years). All subjects participated in a physical activity program. Subjects were randomized to consume a daily nutritional supplement (150 kcal, 20 g whey protein, 800 IU vitamin D, 119 mL beverage) or placebo. In a pre-specified secondary analysis, total-body composition (dual energy X-ray absorptiometry), thigh composition (computed tomography), and muscle strength, power, and quality were assessed before and after the 6-month intervention. After the intervention period, the treatment and placebo groups demonstrated improvements in muscle strength, body composition, and thigh composition. Nutritional supplementation lead to further losses of intermuscular fat and increased normal muscle density compared to the placebo. The authors concluded that nutritional supplementation provided additional benefits to mobility-limited older adults undergoing exercise training.*40

* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

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20. Cermak NM et al. Protein supplementation augments the adaptive response of skeletal muscle to resistance-type exercise training: a meta-analysis. Am J Clin Nutr. 2012; 96(6):1454–64.

21. Morton RW et al. A systematic review, meta-analysis and meta-regression of the effect of protein supplementation on resistance training-induced gains in muscle mass and strength in healthy adults. Br J Sports Med. 2018;52(6):376–84.

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39. Reule CA et.al. Reduced muscular fatigue after a 12-week leucine-rich amino acid supplementation combined with moderate training in elderly: a randomised, placebo-controlled, double-blind trial. BMJ Open Sport Exerc Med. 2017;2:e000156.doi:10.1136/bmjsem-2016-000156

40. Englund DA et al. Nutritional supplementation with physical activity improves muscle composition in mobility-limited older adults, the VIVE2 study: A randomized, double-blind, placebo-controlled trial. J Gerontol A Biol Sci Med Sci. 2018;73:95–101.

41. Howatson G , Hoad M , Goodall S , et al. Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomised, double-blind, placebo-controlled study. J Int Soc Sports Nutr 2012;9:20.